scholarly journals Synthesis, spectral characterization and antimicrobial activity of some M(II) complexes with Ciprofloxacin

2019 ◽  
Vol 10 (2) ◽  
Author(s):  
Emir Horozić ◽  
Amira Cipurković ◽  
Zahida Ademović ◽  
Demir Bjelošević ◽  
Amila Zukić ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Salmonella Enteritidis ◽  
Gram Negative Bacteria ◽  
Bacterial Strains ◽  
Antimicrobial Screening ◽  
Fluoroquinolone Antibiotics ◽  
Wide Range ◽  
The Difference ◽  
Oxygen Donors ◽  
And Staphylococcus Aureus

Ciprofloxacin, CFL is a drug that belongs to the second generation of fluoroquinolone antibiotics with a wide range of effects on Gram-positive and Gram-negative bacteria. The aim of this work was to investigate the interaction of CFL as ligand with divalent biological cations (Mn2+, Ni2+ and Co2+) in approximate physiological conditions. Synthesized complexes were characterized using FTIR and stereo-microscopy. Antimicrobial screening was performed on bacterial strains of Escherichia coli, Salmonella Enteritidis, Enterococcus faecalis and Staphylococcus aureus. The results of FTIR spectroscopy showed that the M(II) complexes with CFL were formed through the oxygen donors of the carboxyl and carbonyl group of the ligand. Stereo-microscopic characterization revealed the difference in color and size of crystals of the ligand and metal complexes. Antimicrobial screening has shown that CFL and complexes have almost similar antimicrobial activity against investigated bacterial strains.

scholarly journals Antimicrobial activity of American cranberry extract in the antimicrobial protection of minced pork meat

2018 ◽  
Vol 74 (3) ◽  
pp. 209-2014
Author(s):  
Agata Stobnicka ◽  
Małgorzata Gniewosz
Keyword(s):  
Antimicrobial Activity ◽  
Sensory Evaluation ◽  
Salmonella Enteritidis ◽  
Gram Negative Bacteria ◽  
Pork Meat ◽  
Bacterial Strains ◽  
Gram Positive ◽  
Gram Negative ◽  
American Cranberry ◽  
Meat Samples

The aim of the study was to determine the content of bioactive ingredients in the American cranberry extracts (Vaccinium macrocarpon), the antimicrobial activity of these extracts and the efficiency of the selected extract in minced pork meat together with its sensory evaluation. Three extracts were made: aqueous (w-FACE), ethanol (e-FACE) and aqueous-ethanol (we-FACE). The content of bioactive components was tested by HPLC. Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) were determined against ten Gram-negative bacteria strains and six Gram-positive bacterial strains by serial macrodilution method. Minced pork meat with or without addition of 2.5% w-FACE was inoculated with four pathogenic strains (Escherichia coli, Salmonella Enteritidis, Listeria monocytogenes and Staphylococcus aureus) and stored at 4°C for 6 days. In 0, 2, 4 and 6 day, the number of bacteria was determined by plate method. Sensory evaluation of meat samples with and without in-FACE included general appearance and smell. All extracts contained organic acids (p-coumarin, benzoic, chlorogenic and coffee) and flavone (quercetin, miristin, epicatechin and isorhamnetin). E-FACE also contains ursolic acid. The MIC/MBC values of the extracts were in the range of 1.56-6.25 (3.13-25.0) mg/ml against Gram-positive bacteria and 6.25-12.5 (6.25-25.0) mg/ml against Gram-negative bacteria. Statistically significant (p ≤ 0.05) inhibition of growth of all pathogens in minced pork meat containing in-FACE was found. The 2.5% w-FACE had no significant effect (p ≤ 0.05) on the initial sensory characteristics of minced pork meat and after 4 and 6 days of storage was significantly higher than that of FACE-free meat. The results of the study suggest the possibility of using an aqueous extract of cranberry fruit as a natural preservative of minced pork meat..

Antimicrobial Activity of Iron-Halide Complexes Against Gram-Positive and Gram-Negative Bacteria

2020 ◽  
Author(s):  
Nusrat Abedin ◽  
Abdullah Hamed A Alshehri ◽  
Ali M A Almughrbi ◽  
Olivia Moore ◽  
Sheikh Alyza ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Antimicrobial Resistance ◽  
Extracellular Dna ◽  
Antibiofilm Activity ◽  
Gram Negative Bacteria ◽  
Bacterial Strains ◽  
Gram Positive ◽  
Gram Negative ◽  
Antimicrobial Substances ◽  
Mammalian Cell Lines

Antimicrobial resistance (AMR) has become one of the more serious threats to the global health. The emergence of bacteria resistant to antimicrobial substances decreases the potencies of current antibiotics. Consequently, there is an urgent and growing need for the developing of new classes of antibiotics. Three prepared novel iron complexes have a broad-spectrum antimicrobial activity with minimum bactericidal concentration (MBC) values ranging from 3.5 to 10 mM and 3.5 to 40 mM against Gram-positive and Gram-negative bacteria with antimicrobial resistance phenotype, respectively. Time-kill studies and quantification of the extracellular DNA confirmed the bacteriolytic mode of action of the iron-halide compounds. Additionally, the novel complexes showed significant antibiofilm activity against the tested pathogenic bacterial strains at concentrations lower than the MBC. The cytotoxic effect of the complexes on different mammalian cell lines show sub-cytotoxic values at concentrations lower than the minimum bactericidal concentrations.

scholarly journals Comparative Study on the Antimicrobial Efficacy of Methanolic Extract of Root, Callus and Fruit Extracts of Myxopyrum smilacifolium Blume

2014 ◽  
Vol 2 (4) ◽  
pp. 521-524
Author(s):  
RP Praveen ◽  
Ashalantha Nair
Keyword(s):  
Antimicrobial Activity ◽  
Methanolic Extract ◽  
Fungal Strain ◽  
Antimicrobial Efficacy ◽  
Bacterial Strains ◽  
E Coli ◽  
Root Extracts ◽  
Fruit Extracts ◽  
Root Callus ◽  
And Staphylococcus Aureus

The aim of the present study was to compare the antimicrobial efficacy of methanolic extract of root, callus and fruit of Myxopyrum smilacifolium Blume. Antimicrobial activity was tested using agar well diffusion with four bacterial strains viz: Escherechia coli, Enterococcus faecalis, Bacillus subtilis and Staphylococcus aureus of which E. coli alone was gram negative. The fungal strain employed was Candida albicans. Root extracts shown to be effective only against B. subtilis. Fruit extracts showed the maximum antimicrobial activity against all the microbial species considered for the current study except against S. aureus. Highlight of the present study was the antimicrobial activity of callus extracts. DOI: http://dx.doi.org/10.3126/ijasbt.v2i4.11362  Int J Appl Sci Biotechnol, Vol. 2(4): 521-524 

scholarly journals Ultrasonic Irradiation: Synthesis, Characterization, and Preliminary Antimicrobial Activity of Novel Series of 4,6-Disubstituted-1,3,5-triazine Containing Hydrazone Derivatives

2016 ◽  
Vol 2016 ◽  
pp. 1-9 ◽  
Author(s):  
Hessa H. Al-Rasheed ◽  
Monirah Al Alshaikh ◽  
Jamal M. Khaled ◽  
Naiyf S. Alharbi ◽  
Ayman El-Faham
Keyword(s):  
Antimicrobial Activity ◽  
Ultrasonic Irradiation ◽  
New Products ◽  
Conventional Heating ◽  
Pyridine Derivative ◽  
Gram Negative Bacteria ◽  
Bacterial Strains ◽  
Gram Positive ◽  
Gram Negative ◽  
Antimicrobial And Antifungal Activities

Novel series of 4,6-disubstituted-1,3,5-triazines containing hydrazone derivatives were synthesized employing ultrasonic irradiation and conventional heating. The ultrasonication gave the target products in higher yields and purity in shorter reaction time compared with the conventional method. IR, NMR (H 1 and C 13), elemental analysis, and LC-MS confirmed the structures of the new products. The antimicrobial and antifungal activities were evaluated for all the prepared compounds against some selected Gram-positive and Gram-negative bacterial strains. The results showed that only two compounds 7i (pyridine derivative) and 7k (4-chlorobenzaldehyde derivative) displayed biological activity against some Gram-positive and Gram-negative bacteria, while the rest of the tested compounds did not display any antifungal activity.

scholarly journals GC/MS Analysis and Antimicrobial Activity of the Essential Oil of Fresh Leaves of Eucalytus Globulus, and Leaves and Stems of Smyrnium Olusatrum from Constantine (Algeria)

2010 ◽  
Vol 5 (10) ◽  
pp. 1934578X1000501 ◽  
Author(s):  
Habiba Daroui-Mokaddem ◽  
Ahmed Kabouche ◽  
Mabrouka Bouacha ◽  
Boudjemaa Soumati ◽  
Aida El-Azzouny ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Essential Oil ◽  
Essential Oils ◽  
Eucalyptus Globulus ◽  
Bacterial Strains ◽  
Ms Analysis ◽  
Wide Range ◽  
Main Components ◽  
Type Apparatus

The essential oils of fresh leaves of Eucalyptus globulus L. (Myrtaceae), and leaves and stems of Smyrnium olusatrum L. (Apiaceae), obtained by hydrodistillation in a Clevenger-type apparatus, were analyzed by GC/MS. Twenty compounds were characterized, representing 98.3% of the essential oil of E. globulus, with 1,8-cineole (48.6%), globulol (10.9%), trans-pinocarveol (10.7%), and α-terpineol (6.6%) as main components, while thirty-three compounds were characterized, representing 94.3% of the essential oil of S. olusatrum, with sabinene (27.1%), curzerene (13.7%), methyl-1-benzyl-2-oxocyclooctane carboxylate (12.3%), α-pinene (7.2%), cryptone (7.1%) and β-pinene (5.7%) as the major components. The essential oils of E. globulus and S. olusatrum were tested against a wide range of fungal and bacterial strains. Both oils showed significant antimicrobial activity.

scholarly journals Synthesis of Cinnamanilide Derivatives and Their Antioxidant and Antimicrobial Activity

2015 ◽  
Vol 2015 ◽  
pp. 1-5 ◽  
Author(s):  
Satish Balasaheb Nimse ◽  
Dilipkumar Pal ◽  
Avijit Mazumder ◽  
Rupa Mazumder
Keyword(s):  
Antimicrobial Activity ◽  
Cinnamic Acid ◽  
Antioxidant Activities ◽  
Gram Negative Bacteria ◽  
Bacterial Strains ◽  
Strong Activity ◽  
Amide Derivatives ◽  
Antioxidant And Antimicrobial Activity ◽  
Derivatives Of ◽  
Strong Antimicrobial Activity

The amide derivatives of cinnamic acid were synthesized and their antimicrobial and antioxidant activities were investigated. The investigation of antimicrobial potentials of the compounds demonstrated a strong activity against 21 bacterial strains comprising Gram-positive and Gram-negative bacteria. Compounds2a,2b, and3bshowed strong antimicrobial activity against all microorganisms with the pMIC value ranging from 2.45 to 3.68. Compounds2a,3a, and3bdemonstrated strong antioxidant activity with % inhibition of the DPPH radical of 51% (±1.14), 41% (±1.01), and 50% (±1.23), respectively. These findings indicate that the amide derivatives of the cinnamic acid possess strong antibacterial and antioxidant activities.

Berberine sulfate: antimicrobial activity, bioassay, and mode of action

1969 ◽  
Vol 15 (9) ◽  
pp. 1067-1076 ◽  
Author(s):  
A. H. Amin ◽  
T. V. Subbaiah ◽  
K. M. Abbasi
Keyword(s):  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Antibacterial Activity ◽  
Deoxyribonucleic Acid ◽  
Test Organism ◽  
Inoculum Size ◽  
Gram Negative Bacteria ◽  
Gram Negative ◽  
Rna And Protein Synthesis ◽  
And Staphylococcus Aureus

Berberine sulfate was shown to possess antimicrobial activity against a wide variety of microorganisms including Gram-positive and Gram-negative bacteria, fungi, and protozoa. The antibacterial activity against Vibrio cholerae and Staphylococcus aureus was dependent on the inoculum size of the test organism and pH of the medium. A method of microbiological assay sensitive to 5–10 μg/ml of the drug was developed. The drug was shown to exert a more rapid antibacterial activity than chloramphenicol and tetracycline on V. cholerae, the K values being 2.4 ×10−2 sec−1, 7.8 × 10−3 sec−1, and 5.2 × 10−3 sec−1 respectively. Berberine sulfate was shown to be bacteriocidal to V. cholerae and bacteriostatic to S. aureus, at concentrations of 35 and 50 μg/ml. In both these organisms concentrations of 35 and 50 μg/ml of the drug inhibited ribonucleic acid (RNA) and protein synthesis almost immediately after the addition of the drug. There was little effect on deoxyribonucleic acid (DNA) synthesis at these concentrations.

scholarly journals Antimicrobial activity of resveratrol and grape pomace extract

10.5219/1054 ◽  
2019 ◽  
Vol 13 (1) ◽  
pp. 363-368 ◽  
Author(s):  
Simona Kunová ◽  
Soňa Felsöciová ◽  
Eva Tvrdá ◽  
Eva Ivanišová ◽  
Atilla Kántor ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Salmonella Enteritidis ◽  
Food Preservation ◽  
Grape Pomace ◽  
Bacterial Strains ◽  
Red Grape ◽  
Red Variety ◽  
Antimicrobial Assay ◽  
Alternative Drugs

Resveratrol is commonly found in food and drinks, including red wine and grapes. Grape extracts have a potent antimicrobial activity in vitro. The antimicrobial activity of plant extracts is the base of their potential application in food preservation agents, pharmaceuticals, cosmetics, alternative drugs and natural therapies. The aim of our study was to evaluate the antimicrobial activity of resveratrol and Blue Frankish pomace extract against Grampositive and Gramnegative bacteria as well as yeasts from the genus Candida. Six bacterial strains (three Grampositive bacteria Staphylococcus aureus CCM 2461, Enterococcus faecalis CCM 4224 and Listeria monocytogenes CCM 4699; three Gramnegative bacteria Escherichia coli CCM 3988, Pseudomonas aeruginosa CCM 1959 and Salmonella enteritidis subsp. enteritidis CCM 4420) and three yeast strains (Candida albicans CCM 8186, Candida krusei CCM 8271 and Candida tropicalis CCM 8223) were evaluated using the antimicrobial assay. Pure resveratrol and grape pomace extracts of red variety Blue Frankish were used. Our results show that resveratrol and red grape pomace extract have a very good antimicrobial activity against Grampositive bacteria when compared with Gramnegative bacteria and yeasts.

scholarly journals Plasmid Transfer by Conjugation in Gram-Negative Bacteria: From the Cellular to the Community Level

Genes ◽  
2020 ◽  
Vol 11 (11) ◽  
pp. 1239
Author(s):  
Chloé Virolle ◽  
Kelly Goldlust ◽  
Sarah Djermoun ◽  
Sarah Bigot ◽  
Christian Lesterlin
Keyword(s):  
Current Knowledge ◽  
Recipient Cell ◽  
Rapid Evolution ◽  
Extensive Study ◽  
Plasmid Transfer ◽  
Gram Negative Bacteria ◽  
Bacterial Strains ◽  
Gram Negative ◽  
Wide Range ◽  
Metabolic Properties

Bacterial conjugation, also referred to as bacterial sex, is a major horizontal gene transfer mechanism through which DNA is transferred from a donor to a recipient bacterium by direct contact. Conjugation is universally conserved among bacteria and occurs in a wide range of environments (soil, plant surfaces, water, sewage, biofilms, and host-associated bacterial communities). Within these habitats, conjugation drives the rapid evolution and adaptation of bacterial strains by mediating the propagation of various metabolic properties, including symbiotic lifestyle, virulence, biofilm formation, resistance to heavy metals, and, most importantly, resistance to antibiotics. These properties make conjugation a fundamentally important process, and it is thus the focus of extensive study. Here, we review the key steps of plasmid transfer by conjugation in Gram-negative bacteria, by following the life cycle of the F factor during its transfer from the donor to the recipient cell. We also discuss our current knowledge of the extent and impact of conjugation within an environmentally and clinically relevant bacterial habitat, bacterial biofilms.

Chemical composition and antimicrobial activity of Erodium species: E. ciconium L., E. cicutarium L., and E. absinthoides Willd. (Geraniaceae)

2010 ◽  
Vol 64 (3) ◽  
Author(s):  
Zorica Stojanović-Radić ◽  
Ljiljana Čomić ◽  
Niko Radulović ◽  
Milan Dekić ◽  
Vladimir Ranđelović ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Chemical Composition ◽  
Essential Oils ◽  
Palmitic Acid ◽  
Gram Negative Bacteria ◽  
Broth Microdilution ◽  
Bacterial Strains ◽  
Gram Negative ◽  
Microdilution Method ◽  
Broth Microdilution Method

AbstractThe present study gives results of chemical composition analyses and antimicrobial activity testing of three Erodium species: E. ciconium L., E. cicutarium L., and E. absinthoides Willd. Essential oils were obtained by hydro-distillation from air-dried entire plants and analyzed by GC and GC-MS. A total of 209 different compounds were identified: 162 for E. cicutarium, 107 for E. ciconium, and 79 for E. absinthoides. Antimicrobial activity (broth microdilution method) of the oils was screened against a panel of Gram positive and Gram negative bacteria and a number of fungi. Moderate susceptibility of all tested strains was observed. Determined MIC values were 0.156–5 mg mL−1 (bacterial strains) and 0.039–0.325 mg mL−1 (fungal strains). Major component of the most active oil, palmitic acid, was also tested for activity together with stearic and myristic acids.

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