scholarly journals Antibacterial, antioxidant, cytotoxic effects and GC-MS analysis of mangrove-derived Streptomyces achromogenes tch4 extract

2021 ◽  
pp. 17-17
Author(s):  
Janpen Tangjitjaroenkun ◽  
Wanchai Pluempanupat ◽  
Rungnapa Tangchitcharoenkhul ◽  
Waraporn Yahayo ◽  
Roongtawan Supabphol
Keyword(s):  
Ethyl Acetate ◽  
Ethyl Acetate Extract ◽  
Biological Activities ◽  
Radical Scavenging ◽  
Bioactive Metabolites ◽  
Total Phenolic ◽  
Anticancer Activities ◽  
Antioxidant Power ◽  
Dry Extract

The in vitro biological activities of the ethyl acetate extract of the culture filtrate from Streptomyces achromogenes TCH4 (TCH4 extract) were evaluated. The ethyl acetate extract of TCH4 produced a bacteriostatic effect against Enterobacter cloacae, Staphylococcus aureus, Staphylococcus saprophyticus, Bacillus subtilis, methicillin-resistant S. aureus (MRSA) and Klebsiella pneumoniae. The extract had bactericidal activity against S. aureus, S. saprophyticus, S. aureus (MRSA) and K. pneumoniae with minimum bactericidal concentration (MBC) values in the range of 500-1000 ?g/mL. The total phenolic and flavonoid contents in TCH4 were 107.20?2.57 mg gallic acid equivalent (GAE)/g and 44.91?0.84 mg quercetin equivalent (QE)/g of dry extract. Antioxidant activity was determined by DPPH radical (IC50 299.64?6.83 ?g/mL) and ABTS radical scavenging (IC50 65.53?0.95 ?g/mL), and the ferric-reducing antioxidant power (FRAP) (822.76?9.12 mM FeSO4.7H2O/g dry extract) assays. TCH4 exhibited cytotoxic activity in the DU-145 cell line (IC50 9.36?0.37 ?g/mL). Analysis of extract constituents by GC-MS revealed pyrrolo[1,2-a]pyrazine-1,4-dione, hexahydro-3-(2-methylpropyl) (36.85%), benzeneacetamide (23.76%), and deferoxamine (12.85%) as the major compounds, which have been reported to possess pharmaceutical properties. S. achromogenes TCH4 could be a potential source of bioactive metabolites with antibacterial, antioxidant and anticancer activities for pharmaceutical applications.

Biomedical applications of fish gut associated Streptomyces maritimus IM20 isolated from Rastrelliger kanagurta (Indian mackerel)

2021 ◽  
Vol 16 (10) ◽  
pp. 120-130
Author(s):  
Angamuthu Vignesh ◽  
Venugopal Gopikrishnan ◽  
Sivaraj Anbarasu ◽  
Manikkam Radhakrishnan ◽  
Joseph Jerrine
Keyword(s):  
Ethyl Acetate ◽  
Ethyl Acetate Extract ◽  
Radical Scavenging ◽  
Bioactive Metabolites ◽  
Molecular Characteristics ◽  
Inhibition Assay ◽  
Indian Mackerel ◽  
Fish Gut ◽  
Rastrelliger Kanagurta

The biomedical potential of fish gut-associated actinobacteria isolated from the marine fish Rastrelliger kanagurta (Indian mackerel) was investigated. The actinobacterial strain IM20 was isolated from the fish gut by using Kusters agar medium prepared with 50% sea water. Based on their phenotypic and molecular characteristics, strain IM20 was identified as Streptomyces maritimus. The bioactive metabolites produced from the strain IM20 by agar surface fermentation and ethyl acetate extraction were tested for in vitro antimicrobial, antiquorum sensing , anti-biofilm, anti TB, anti-oxidant and anti-cancer activity. The MIC value of ethyl acetate extract (EAE) of IM20 was found to be 16 μg ml−1 against S. aureus and E. coli. In quorum sensing inhibition assay, the extract showed violacein inhibition upto 87% at 512 μg ml−1 concentration when tested by pigment inhibition assay using C. violaceum MTCC 2656. The results of in vitro assays revealed that the ethyl acetate extract of IM-20 (EAE-IM20) showed 75%, 83% and 72% inhibition against Mycobacterium tuberculosis H37Rv, M. tuberculosis (SHRE sensitive) and multi drug resistant (MDR) M tuberculosis respectively at 500 μg ml−1 concentration. In DPPH assay, 71% radical scavenging activity was exhibited by the EAE-IM20 at 250 μg ml−1. In MTT assay, EAEIM20 exhibited 74.29±2.01% inhibition on breast cancer cell line MCF7 (250 μg ml−1). This study broadly determines that the fish associated actinobacteria is a prolific place for diverse multifunctional bioactive compounds for the development of medically important unique drugs.

scholarly journals Phenolic content and antioxidant activity of Phymatopteris hastata

2011 ◽  
Vol 76 (11) ◽  
pp. 1485-1496 ◽  
Author(s):  
Wei Su ◽  
Peiyuan Li ◽  
Lini Huo ◽  
Caiying Wu ◽  
Nana Guo ◽  
...  
Keyword(s):  
Ethyl Acetate ◽  
Ethyl Acetate Extract ◽  
Antioxidant Activities ◽  
Petroleum Ether Extract ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Butylated Hydroxytoluene ◽  
Total Phenolic ◽  
Alcohol Extract

Various solvent extracts of Phymatopteris hastata, a traditional Chinese medicinal material, were screened for their antioxidant activities. Four systems of in vitro testing were employed to investigate the antiradical and antioxidant effect, i.e., the 2,2-diphenyl-1-picryhydrazyl (DPPH) and 2,2'-azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) systems, the hydroxyl radical scavenging assay and the reducing power. In addition, butylated hydroxytoluene (BHT), a widely used synthetic antioxidant, was also studied for comparison. The results revealed that the ethyl acetate extract exhibited outstanding antioxidant activities, which was close or even superior to BHT. Furthermore, the total phenolic (TP) and total flavonoid (TF) contents of different extracts were measured, expressed as gallic acid and rutin equivalent, respectively. The antioxidant activities and the TP/TF content of different extracts followed the same order: ethyl acetate extract > butyl alcohol extract > petroleum ether extract, showing a good correlation between the antioxidant activities and the TP/TF content. The results showed that these extracts, especially the ethyl acetate extract, were rich in phenolics and flavonoids and could be considered as natural antioxidants.

Antioxidant Activities of Mirabilis jalapa L. Seed Epicarp Extract

2012 ◽  
Vol 550-553 ◽  
pp. 1768-1772
Author(s):  
Xin Hong Wang ◽  
Jing Tao Dai
Keyword(s):  
Free Radical ◽  
Ethyl Acetate ◽  
Ethyl Acetate Extract ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Natural Antioxidants ◽  
Total Phenolic ◽  
Frap Assay ◽  
Antioxidant Power ◽  
Mirabilis Jalapa

Mirabilis jalapa L. (Nyctaginaceae) is a traditional specie and widely distributed in most parts of China. Its leaves, roots, seed and flowers are used as drugs pharmacological activity. This is the first report on the biological activity against activities of scavenging free radical and ferric reducing antioxidant power (FRAP). Mirabilis jalapa L. seed epicarp (MJSE) was immersed with ethanol (or methanol, ethyl acetate, water) in the ultrasonic instrument, then determination of total phenolic content (TPC) and total flavonoid content (TFC) of these extracts were performed. Furthermore, antioxidant activities of these extracts were evaluated by using DPPH radical scavenging, hydroxyl radical scavenging and FRAP. The TPC, TFC, FRAP and scavenging activities against DPPH and OH radical were in the following order ethyl acetate >ethanol>methanol>water. The TPC of ethyl acetate extract is 4.24 mg GAE /g of dry power weight (DPW), and TFC 0.39 mg RE/ g DPW. ethyl acetate extract exhibit high free radical scavenging rate, IC50 of DPPH and OH assay were 6.62 mg DPW/ml and 3.49 mg DPW/ml, respectively. The TPC values, IC50 obtained from DPPH assay and FRAP assay were highly correlated with correlation of determination (R2 = 0.9878, R2 = 0.9419). The present study confirmed that MJSE has a strong antioxidant activity and can be considered to be safe and an inexpensive source of natural antioxidants and drugs.

scholarly journals In-Vitro Antioxidant Activity and Total Phenolic Content of Ruta montana L. Extracts

2020 ◽  
Vol 10 (2) ◽  
pp. 69-75
Author(s):  
Mounira Merghem ◽  
Saliha Dahamna
Keyword(s):  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Ethyl Acetate Extract ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Chloroform Extract ◽  
Total Phenolic ◽  
Ruta Montana ◽  
Β Carotene

The aim of this study is to evaluate in vitro antioxidant activities of Ruta montana L.  extracts. This activity was evaluated by three methods : DPPH (2, 2'-diphenyl- 1- picrylhydrazy), bleaching of β-carotene and chelation of ferrous iron. Results showed that ethyl acetate extract (EAE) represents the highest amount of total polyphenols, tannins and flavonoids with 257,1 ± 0,703µg gallic acid equivalent/mg of extract,  251 ± 1.41 µg tannic acid equivalent /mg of extract,117,4 ± 3,451 µg quercetin equivalents/mg of extract, 139,5 ± 4,107 µg rutin equivalents/mg of extract, respectively. In the DPPH assay, ethyl acetate extract showed the higher scavenging capacity (IC50 = 0.044 ± 0.001 mg/ml) followed by methanol, aqueous and chloroform extract. Whereas, AqE showed the best chelating effect and the best inhibitory capacity of the coupled oxidation of linoleic acid/ β-carotene. Keywords: Ruta montana L; polyphenols; antioxidant activity; free radical scavenging.

Phytochemical screening, in vitro antioxidant and enzyme inhibitory proprieties and acute toxicity of extracts from the aerial parts of Ephedra nebrodensis, source of bioactive compounds

2021 ◽  
Vol 24 ◽  
Author(s):  
Meriem Hamoudi ◽  
Djouher Amroun ◽  
Sabrina Boutefnouchet ◽  
Chawki Bensouici ◽  
Samira Kaoula ◽  
...  
Keyword(s):  
Acute Toxicity ◽  
Ethyl Acetate ◽  
Bioactive Compounds ◽  
Ethyl Acetate Extract ◽  
Radical Scavenging ◽  
Mass Spectrometric ◽  
Mass Spectrometric Detection ◽  
Total Phenolic ◽  
Aerial Parts

Background: Due to the strong association between the chemistry of medicinal plants and their biological properties, it is important to determine their phytochemical composition to justify experimental tests. Objective: The aim of this study was to evaluate the in vitro antioxidant and the enzyme inhibitory properties and to identify the bioactive compounds present in the extracts of Ephedra nebrodensis growing in Algeria. Methods: Total phenolic and flavonoids content in these extracts were quantified by Folin-Ciocalteu and aluminum chloride methods. The antioxidant capacity was assessed using DPPH, ABTS, β-carotene/linoleic acid, CUPRAC and FRAP assays and in vitro cholinesterase activity against acetylcholinesterase and butyrylcholinesterase were evaluated. The chemical constituents of the extracts were analyzed by high-performance liquid chromatography coupled to mass spectrometric detection and gas chromatography coupled to mass spectrometric detection. For the acute toxicity study, extracts were administered to mice at single dose of 2 g/kg and 5 g/kg by gavage. Results: Plant extracts were rich in phenolic compounds. Ethyl acetate extract presented the highest phenolic (238.44 ± 1.50 µg GAE /mg of extract) and flavonoid (21.12 ± 0.00 µg QE /mg of extract) contents. Likewise, ethyl acetate extract showed potent radical scavenging and reducing properties. Ethanol: acetone extract showed inhibitory activity against acetylcholinesterase, and was a potent inhibitor of butyrylcholinesterase. In all extracts, flavonoids were the most abundant compounds. The phytochemical investigation showed the presence of alkaloids (ephedrine and pseudo-ephedrine). In the acute toxicity, the LD50 was superior to 5 g/kg body weight. There are not alterations in the histology of the liver and kidneys. Conclusion: This study demonstrated a good antioxidant potential and anticholinesterase activity of aerial parts of E. nebrodensis.

scholarly journals A Comparative Study on Antioxidation and Antibacterial Activities Triphala Herb Extracts from Chae Son, Lampang, Thailand

2020 ◽  
pp. 1-14
Author(s):  
Wilart Pompimon ◽  
Wipanoot Baison ◽  
Angkhana Chuajedton ◽  
Phansuang Udomputtimekakul ◽  
Punchavee Sombutsiri ◽  
...  
Keyword(s):  
Ethyl Acetate ◽  
Methanol Extract ◽  
Ethyl Acetate Extract ◽  
Biological Activities ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Inhibition Zone ◽  
Antibacterial Activities ◽  
Antioxidant Power ◽  
Methanolic Extracts

Introduction: Triphala, a herbal medicine which is a product from Chae Son, Lampang, Thailand, is an unequal-proportional mixture of fruits of three herbs, Phyllanthus emblica, Terminalia chebula, and Terminalia bellirica (2:1:1). Materials and Methods: The contemporary study concentrated on extracts detection and comparative analysis between various annual year (A.D.2017/2018) activities in hexane, ethyl acetate, and methanolic extracts of Triphala. The determination of antioxidant was using by 1,1-diphenyl-2-picrylhydrazyl (DPPH) and ferric reducing antioxidant power (FRAP). The broth dilution and agar well diffusion assay were determined antibacterial. Results: Results exposed the valuable biological activity extracts from ethyl acetate and methanol solvents of T. chebula and T. bellirica, which strength is accountable for biological activities. Extracts displayed acceptable radical-scavenging activity analogous with ascorbic acid. The methanol extract of T. bellirica (0.0013 mg/mL), ethyl acetate extract of P. emblica (0.0009 mg/mL), in A.D. 2017 and the ethyl acetate extract of P. emblica (0.0090 mg/mL), the methanol extract of Triphala (0.0176 mg/mL) in 2018, were exhibited higher antioxidant activity. Also, extracts indicated hopeful antibacterial potential against tested strain comparable to chloramphenicol. Conclusion: The results in A.D. 2017 showed that inhibition zone of P. emblica in ethyl acetate extract; S. aureas (8.67 mm), E. aerogenes (8.67 mm), E. coli (EPEC) (7.67 mm), P. mirabilis (7.73 mm) , S. typhimurium (8.33 mm), S. flexneri (8.33 mm) and V. cholera (7.33 mm) together with P. emblica methanol extract; S. aureas (6.67 mm), E. aerogenes (7.76 mm.)  and V. cholera (4.0 mm). Therefore, it could be concluded that Triphala may be a talented candidate in pharmaceutical and future medicine.

scholarly journals Chemical Profiling and Biological Evaluation of Nepeta baytopii Extracts and Essential Oil: An Endemic Plant from Turkey

Plants ◽  
2021 ◽  
Vol 10 (6) ◽  
pp. 1176
Author(s):  
Gokhan Zengin ◽  
Mohamad Fawzi Mahomoodally ◽  
Abdurrahman Aktumsek ◽  
József Jekő ◽  
Zoltán Cziáky ◽  
...  
Keyword(s):  
Essential Oil ◽  
Ethyl Acetate ◽  
Biological Activities ◽  
Radical Scavenging ◽  
Biological Evaluation ◽  
Endemic Plant ◽  
Antioxidant Power ◽  
Water Extracts ◽  
Trolox Equivalent

Nepeta baytopii is a poorly studied, endemic Nepeta species (Lamiaceae) of Turkey. For the first time, the biological activities (antioxidant, enzyme inhibition, and cytotoxicity properties) of the hexane, ethyl acetate, methanol, water/methanol, and water extracts and essential oil prepared from N. baytopii aerial parts were assessed. Hydro-methanol (41.25 mg gallic acid equivalent (GAE)/g) and water extracts (50.30 mg GAE/g), respectively showed the highest radical scavenging (94.40 and 129.22 mg Trolox equivalent (TE)/g, for 2,2-diphenyl-1-picrylhydrazyl radical and 2,2-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid radical scavenging assays) and reducing (229.37 and 129.55 mg TE/g, for ferric-reducing antioxidant power and cupric-reducing antioxidant capacity assays) capacities in vitro. An interestingly high inhibition was observed for ethyl acetate extract against butyrylcholinesterase (10.85 mg galantamine equivalent/g). The methanol extract showed high cytotoxicity (31.7%) against HepG2 cells. Caryophyllene oxide was identified in high concentrations in the essential oil (39.3%). Luteolin and apigenin and their derivatives were identified from the methanol and water extracts. The results obtained from this study highlighted that the abundance of highly bioactive compounds from Nepeta baytopii ensures the multiple biological activities of the tested extracts, and this suggests a potential use in the pharmaceutical and nutraceutical fields, and therefore should be investigated further.

scholarly journals Green-Sustainable Recovery of Phenolic and Antioxidant Compounds from Industrial Chestnut Shells Using Ultrasound-Assisted Extraction: Optimization and Evaluation of Biological Activities In Vitro

Antioxidants ◽  
2020 ◽  
Vol 9 (3) ◽  
pp. 267
Author(s):  
Fátima Lameirão ◽  
Diana Pinto ◽  
Elsa F. Vieira ◽  
Andreia F. Peixoto ◽  
Cristina Freire ◽  
...  
Keyword(s):  
Biological Activities ◽  
Radical Scavenging ◽  
Raw Material ◽  
Total Phenolic ◽  
Antioxidant Compounds ◽  
Ultrasound Assisted Extraction ◽  
Antioxidant Power ◽  
Assisted Extraction ◽  
Ultrasound Assisted

Chestnut processing industry generates large amounts of by-products, including leaves, burs and shells that are a source of bioactive compounds. The purpose of this study was to establish an ultrasound-assisted extraction (UAE) of phenolic and antioxidant compounds from industrial chestnut shells. A central composite design (CCD) was conducted to analyze the effects of time (4–46 min) and temperature (34–76 °C) in the antioxidant activity (2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid (ABTS), 2,2-diphenyl-1-picrylhydrazyl (DPPH), and ferric reducing antioxidant power (FRAP)) and total phenolic compounds (TPC) of chestnut shells extracts. The optimal extraction conditions were obtained at 70 °C for 40 min. The optimal extract was characterized regarding phenolic profile, radical scavenging capacity, and effects on intestinal and dermal cell lines. The optimal extract revealed high amounts of ellagic acid (40.4 µg/mg dw), followed by caffeic acid derivative (15.4 µg/mg dw) and epigallocatechin (15.3 µg/mg dw). Indeed, the extract exhibited the highest scavenging efficiencies against NO● (IC50 = 0.1 µg/mL) and HOCl (IC50 = 0.7 µg/mL) and did not conducted to a decrease on HaCaT and HFF-1 viability up to 100 μg/mL. Oppositely, a decrease on Caco-2 and HT29-MTX viability was observed. This study suggests that UAE could be a sustainable option to valorize chestnut shells as raw material for different industries.

scholarly journals Antioxidant Activity, α-Glucosidase Inhibition and UHPLC–ESI–MS/MS Profile of Shmar (Arbutus pavarii Pamp)

Plants ◽  
2021 ◽  
Vol 10 (8) ◽  
pp. 1659
Author(s):  
Nawal Buzgaia ◽  
Soo Yee Lee ◽  
Yaya Rukayadi ◽  
Faridah Abas ◽  
Khozirah Shaari
Keyword(s):  
Free Radical ◽  
Ethyl Acetate ◽  
Biological Activities ◽  
Radical Scavenging ◽  
Stem Bark ◽  
Total Phenolic ◽  
Antioxidant Power ◽  
Esi Ms ◽  
Crude Extracts ◽  
Inhibitory Activities

The genus Arbutus (Ericaceae) has been traditionally used in folk medicine due to its phytomedicinal properties, especially Arbutus pavarii Pamp. However, this plant has not been evaluated for its efficacy, quality, and consistency to support the traditional uses, potentially in treating diabetes. Despite previous studies that revealed the biological activities of A. pavarii as antioxidant and α-glucosidase inhibitory agents, scientific reports on the bioactive compounds that contribute to its health benefits are still scarce. Therefore, this research focused on the evaluation of antioxidant and α-glucosidase inhibitory activities of the methanol crude extracts and various fractions of the leaf and stem bark, as well as on metabolite profiling of the methanol crude extracts. The extracts and fractions were evaluated for total phenolic (TPC) and total flavonoid (TFC) contents, as well as the DPPH free radical scavenging, ferric reducing antioxidant power (FRAP), and α-glucosidase inhibitory activities. Methanol crude extracts of the leaf and stem bark were then subjected to UHPLC–ESI–MS/MS. To the best of our knowledge, the comparative evaluation of the antioxidant and α-glucosidase inhibitory activities of the leaf and stem bark of A. pavarii, as well as of the respective solvent fractions, is reported herein for the first time. Out of these extracts, the methanolic crude extracts and polar fractions (ethyl acetate and butanol fractions) showed significant bioactivities. The DPPH free radical and α-glucosidase inhibitions was highest in the leaf ethyl acetate fraction, with IC50 of 6.39 and 4.93 µg/mL, respectively, while the leaf methanol crude extract and butanol fraction exhibited the highest FRAP with 82.95 and 82.17 mmol Fe (II)/g extract. The UHPLC–ESI–MS/MS analysis resulted in the putative identification of a total of 76 compounds from the leaf and stem bark, comprising a large proportion of plant phenolics (flavonoids and phenolic acids), terpenoids, and fatty acid derivatives. Results from the present study showed that the different parts of A. pavarii had potent antioxidant and α-glucosidase inhibitory activities, which could potentially prevent oxidative damage or diabetes-related problems. These findings may strengthen the traditional claim on the medicinal value of A. pavarii.

scholarly journals Secondary metabolite content and in vitro biological effects of Ajuga chamaepitys (L.) Schreb. subsp. chamaepitys

2015 ◽  
Vol 67 (4) ◽  
pp. 1195-1202 ◽  
Author(s):  
Dragana Jakovljevic ◽  
Sava Vasic ◽  
Milan Stankovic ◽  
Ljiljana Comic ◽  
Marina Topuzovic
Keyword(s):  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Ethyl Acetate Extract ◽  
Biological Activities ◽  
Biological Effects ◽  
Antimicrobial Activities ◽  
Acetone Extract ◽  
Flowering Plant ◽  
Total Phenolic

The antioxidant and antimicrobial activities and contents of total phenolics and flavonoids of Ajuga chamaepitys (L.) Schreb. subsp. chamaepitys (Lamiaceae) were investigated. Five different extracts from aboveground flowering plant parts were obtained by extraction with water, methanol, acetone, ethyl acetate and petroleum ether. The total phenolic content was determined spectrophotometrically using the Folin-Ciocalteu reagent and expressed as the gallic acid equivalent (mg GA/g of extract). The highest value was obtained in the ethyl acetate extract (57.02 mg GA/g). The concentration of flavonoids, determined using a spectrophotometric method with aluminum chloride and expressed as the rutin equivalent (mg RU/g of extract), was highest in the ethyl acetate extract (91.76 mg RU/g). The antioxidant activity was determined in vitro using 2,2-diphenyl-1-picrylhydrazyl (DPPH) reagent. The highest antioxidant activity was detected in the acetone extract (SC50 value = 330.52 ?g/mL). In vitro antimicrobial activities were determined using a microdilution method, and the minimum inhibitory concentration (MIC) and minimum microbicidal concentration (MMC) were determined. The most effective antimicrobial activity against Bacillus cereus was demonstrated by the acetone extract, with MIC and MMC values of 1.25 mg/mL. Based on the results of this study, A. chamaepitys subsp. chamaepitys could be considered as a valuable source of natural compounds with important biological activities.

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