scholarly journals Directing visceral white adipocyte precursors to a thermogenic adipocyte fate improves insulin sensitivity in obese mice

eLife ◽  
2017 ◽  
Vol 6 ◽  
Author(s):  
Chelsea Hepler ◽  
Mengle Shao ◽  
Jonathan Y Xia ◽  
Alexandra L Ghaben ◽  
Mackenzie J Pearson ◽  
...  
Keyword(s):  
Insulin Resistance ◽  
Significant Risk ◽  
Obese Mice ◽  
White Adipocyte ◽  
Genetic Ablation ◽  
Subcutaneous White Adipose Tissue ◽  
Novel Strategy ◽  
Thermogenic Adipocytes ◽  
Visceral Adipose ◽  
Transcriptional Suppressor

Visceral adiposity confers significant risk for developing metabolic disease in obesity whereas preferential expansion of subcutaneous white adipose tissue (WAT) appears protective. Unlike subcutaneous WAT, visceral WAT is resistant to adopting a protective thermogenic phenotype characterized by the accumulation of Ucp1+ beige/BRITE adipocytes (termed ‘browning’). In this study, we investigated the physiological consequences of browning murine visceral WAT by selective genetic ablation of Zfp423, a transcriptional suppressor of the adipocyte thermogenic program. Zfp423 deletion in fetal visceral adipose precursors (Zfp423loxP/loxP; Wt1-Cre), or adult visceral white adipose precursors (PdgfrbrtTA; TRE-Cre; Zfp423loxP/loxP), results in the accumulation of beige-like thermogenic adipocytes within multiple visceral adipose depots. Thermogenic visceral WAT improves cold tolerance and prevents and reverses insulin resistance in obesity. These data indicate that beneficial visceral WAT browning can be engineered by directing visceral white adipocyte precursors to a thermogenic adipocyte fate, and suggest a novel strategy to combat insulin resistance in obesity.

scholarly journals Lyophilized Maqui (Aristotelia chilensis) Berry Induces Browning in the Subcutaneous White Adipose Tissue and Ameliorates the Insulin Resistance in High Fat Diet-Induced Obese Mice

Antioxidants ◽  
2019 ◽  
Vol 8 (9) ◽  
pp. 360 ◽  
Author(s):  
Viviana Sandoval ◽  
Antoni Femenias ◽  
Úrsula Martínez-Garza ◽  
Hèctor Sanz-Lamora ◽  
Juan Castagnini ◽  
...  
Keyword(s):  
Insulin Resistance ◽  
Adipose Tissue ◽  
White Adipose Tissue ◽  
High Fat Diet ◽  
Binding Protein ◽  
Fibroblast Growth Factor 21 ◽  
Obese Mice ◽  
High Fat ◽  
Subcutaneous White Adipose Tissue ◽  
Aristotelia Chilensis

Maqui (Aristotelia Chilensis) berry features a unique profile of anthocyanidins that includes high amounts of delphinidin-3-O-sambubioside-5-O-glucoside and delphinidin-3-O-sambubioside and has shown positive effects on fasting glucose and insulin levels in humans and murine models of type 2 diabetes and obesity. The molecular mechanisms underlying the impact of maqui on the onset and development of the obese phenotype and insulin resistance was investigated in high fat diet-induced obese mice supplemented with a lyophilized maqui berry. Maqui-dietary supplemented animals showed better insulin response and decreased weight gain but also a differential expression of genes involved in de novo lipogenesis, fatty acid oxidation, multilocular lipid droplet formation and thermogenesis in subcutaneous white adipose tissue (scWAT). These changes correlated with an increased expression of the carbohydrate response element binding protein b (Chrebpb), the sterol regulatory binding protein 1c (Srebp1c) and Cellular repressor of adenovirus early region 1A–stimulated genes 1 (Creg1) and an improvement in the fibroblast growth factor 21 (FGF21) signaling. Our evidence suggests that maqui dietary supplementation activates the induction of fuel storage and thermogenesis characteristic of a brown-like phenotype in scWAT and counteracts the unhealthy metabolic impact of an HFD. This induction constitutes a putative strategy to prevent/treat diet-induced obesity and its associated comorbidities.

Nr2e1 deficiency aggravates insulin resistance and chronic inflammation of visceral adipose tissues in a diet-induced obese mice model

Life Sciences ◽  
2021 ◽  
Vol 278 ◽  
pp. 119562
Author(s):  
Guangzhen He ◽  
Jiaowei Gu ◽  
Huawei Wang ◽  
Siyuan Cheng ◽  
Qing Xiong ◽  
...  
Keyword(s):  
Insulin Resistance ◽  
Chronic Inflammation ◽  
Obese Mice ◽  
Mice Model ◽  
Adipose Tissues ◽  
Visceral Adipose

Topical Application of Capsaicin Reduces Visceral Adipose Fat by Affecting Adipokine Levels in High-Fat Diet-Induced Obese Mice

Obesity ◽  
2012 ◽  
Author(s):  
Gong-Rak Lee ◽  
Mi Kyung Shin ◽  
Dong-Joon Yoon ◽  
Ah-Ram Kim ◽  
Rina Yu ◽  
...  
Keyword(s):  
Topical Application ◽  
High Fat Diet ◽  
Obese Mice ◽  
High Fat ◽  
Visceral Adipose

Liver-Specific Overexpression of NOS1AP Alleviates Hepatic Insulin Resistance in Obese Mice

Diabetes ◽  
2018 ◽  
Vol 67 (Supplement 1) ◽  
pp. 1850-P
Author(s):  
CHEN WANG ◽  
KAIDA MU ◽  
TIANXUE ZHAO ◽  
HUI ZHU ◽  
WEIPING JIA
Keyword(s):  
Insulin Resistance ◽  
Hepatic Insulin Resistance ◽  
Obese Mice

1944-P: Muscle a-Parvin Is Not Necessary for Integrin-Linked Kinase Related Insulin Resistance in Diet-Induced Obese Mice

Diabetes ◽  
2019 ◽  
Vol 68 (Supplement 1) ◽  
pp. 1944-P
Author(s):  
DAVID A. CAPPEL ◽  
AMBRA POZZI ◽  
ROY ZENT ◽  
DAVID WASSERMAN
Keyword(s):  
Insulin Resistance ◽  
Obese Mice ◽  
Integrin Linked Kinase

scholarly journals Systems-wide effects of short-term feed deprivation in obese mice

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Daniel Andersen ◽  
Henrik Munch Roager ◽  
Li Zhang ◽  
Janne Marie Moll ◽  
Henrik Lauritz Frandsen ◽  
...  
Keyword(s):  
Adipose Tissue ◽  
Butyric Acid ◽  
Visceral Adipose Tissue ◽  
Animal Studies ◽  
Uncoupling Protein ◽  
Metabolic Changes ◽  
Obese Mice ◽  
Short Term ◽  
Feed Deprivation ◽  
Visceral Adipose

AbstractWhile prolonged fasting induces significant metabolic changes in humans and mice, less is known about systems-wide metabolic changes in response to short-term feed deprivation, which is used in experimental animal studies prior to metabolic challenge tests. We here performed a systems biology-based investigation of connections between gut bacterial composition and function, inflammatory and metabolic parameters in the intestine, liver, visceral adipose tissue, blood and urine in high-fat fed, obese mice that were feed deprived up to 12 h. The systems-wide analysis revealed that feed deprivation linked to enhanced intestinal butyric acid production and expression of the gene encoding the pro-thermogenic uncoupling protein UCP1 in visceral adipose tissue of obese mice. Ucp1 expression was also positively associated with Il33 expression in ileum, colon and adipose tissue as well as with the abundance of colonic Porphyromonadaceae, the latter also correlating to cecal butyric acid levels. Collectively, the data highlighted presence of a multi-tiered system of inter-tissue communication involving intestinal, immune and metabolic functions which is affected by feed deprivation in obese mice, thus pointing to careful use of short-feed deprivation in metabolic studies using obese mice.

scholarly journals The Prevalence of Insulin Resistance and the Associated Risk Factors in a Sample of 14–18-Year-Old Slovak Adolescents

2021 ◽  
Vol 18 (3) ◽  
pp. 909
Author(s):  
Jana Jurkovičová ◽  
Katarína Hirošová ◽  
Diana Vondrová ◽  
Martin Samohýl ◽  
Zuzana Štefániková ◽  
...  
Keyword(s):  
Physical Activity ◽  
Risk Factors ◽  
Insulin Resistance ◽  
Venous Blood ◽  
Significant Risk ◽  
Lifestyle Changes ◽  
Skewed Distribution ◽  
Model Assessment ◽  
Lifestyle Risk Factors ◽  
Lifestyle Risk

The prevalence of cardiometabolic risk factors has increased in Slovakian adolescents as a result of serious lifestyle changes. This cross-sectional study aimed to assess the prevalence of insulin resistance (IR) and the associations with cardiometabolic and selected lifestyle risk factors in a sample of Slovak adolescents. In total, 2629 adolescents (45.8% males) aged between 14 and 18 years were examined in the study. Anthropometric parameters, blood pressure (BP), and resting heart rate were measured; fasting venous blood samples were analyzed; and homeostasis model assessment (HOMA)-insulin resistance (IR) was calculated. For statistical data processing, the methods of descriptive and analytical statistics for normal and skewed distribution of variables were used. The mean HOMA-IR was 2.45 ± 1.91, without a significant sex differences. IR (cut-off point for HOMA-IR = 3.16) was detected in 18.6% of adolescents (19.8% males, 17.6% females). IR was strongly associated with overweight/obesity (especially central) and with almost all monitored cardiometabolic factors, except for total cholesterol (TC) and systolic BP in females. The multivariate model selected variables such as low level of physical fitness, insufficient physical activity, breakfast skipping, a small number of daily meals, frequent consumption of sweetened beverages, and low educational level of fathers as significant risk factors of IR in adolescents. Recognizing the main lifestyle risk factors and early IR identification is important in terms of the performance of preventive strategies. Weight reduction, regular physical activity, and healthy eating habits can improve insulin sensitivity and decrease the incidence of metabolic syndrome, type 2 diabetes, and cardiovascular disease (CVD).

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