scholarly journals Human papillomavirus infection and follow-up on positive results in 7222 female samples obtained from 2016 to 2019 in Hefei, China

PeerJ ◽  
2020 ◽  
Vol 8 ◽  
pp. e10179
Author(s):  
Liduo Peng ◽  
Liping Yin ◽  
Yaqian Dai ◽  
Yuanjing Peng ◽  
Yuanhong Xu ◽  
...  
Keyword(s):  
Human Papillomavirus ◽  
High Risk ◽  
Age Groups ◽  
Hpv Infection ◽  
Single Infection ◽  
Cervical Lesions ◽  
Positive Rate ◽  
Cervical Cells ◽  
Risk Infection

Background Human papillomavirus (HPV) infection rates in women vary regionally. This study analyzed HPV infection in women of different age groups in Hefei, China, performed follow-up on positive cases, and discussed infection prognoses. Methods Samples (7,222) of exfoliated cervical cells were collected in Hefei and tested with an HPV assay kit against 27 HPV genotypes. Statistical software was used to analyze the data. Results The total positive rate of infection was 17.13% (1,068 women), and the 51–60-year age group had the highest HPV infection rate (19.82%). There were statistically significant differences between rates in the 21–30 and 31–40 (P = 0.002), 21–30 and 41–50 (P = 0.0003), 21–30 and 51–60 (P = 0.00003), and 51–60 and >60 age groups (P = 0.046). High-risk infection (15.67%) and single infection (13.01%) were the main types of HPV infection. The dominant genotypes of high-risk infection were HPV 52 (2.42%), HPV 16 (2.01%), HPV 53 (1.43%), HPV 58 (1.32%) and HPV 66 (1.01%). We conducted follow-up on cases in 69 of 94 women who had a history of 1–4 years of positive infection, and in 18 (seven treated, 11 untreated) patients, infection status turned negative (26.09%). Seventeen of the fifty-one women whose infections did not turn negative received treatment. Persistent infection was predominantly observed in high-risk genotypes (56 of 69). Conclusions The results recommend that women in Hefei improve health awareness and receive a 9-valent vaccine. Additionally, women with persistent infections should consult a gynecologist to prevent cervical lesions.

scholarly journals IL-6 and IL-10 in the serum and exfoliated cervical cells of patients infected with high-risk human papillomavirus

PLoS ONE ◽  
2021 ◽  
Vol 16 (3) ◽  
pp. e0248639
Author(s):  
Camila Mareti Bonin-Jacob ◽  
Larissa Zatorre Almeida-Lugo ◽  
Marco Antonio Moreira Puga ◽  
Ana Paula Machado ◽  
Cacilda Tezelli Junqueira Padovani ◽  
...  
Keyword(s):  
Human Papillomavirus ◽  
High Risk ◽  
Uterine Cervix ◽  
Hpv Infection ◽  
Single Infection ◽  
Multiple Infections ◽  
Neoplastic Progression ◽  
Cervical Lesions ◽  
Hpv Dna ◽  
Cervical Cells

Persistent infection by high-risk human papillomavirus (HR-HPV) is the main cause of cervical cancer and its precursor lesions. While some cytokines help immune cells in virus clearance, others contribute to the persistence of infection and neoplastic progression. Here, the levels of interferon (IFN)-γ, tumor necrosis factor (TNF)-α, interleukin (IL)-10, IL-6, IL-4, and IL-2 were quantified in the serum and exfoliated cervical cells (ECCs) of patients with HR-HPV, and the presence of IL-6+ cells was investigated in uterine cervix biopsies. Cytokine levels in the serum and ECCs of 26 HR-HPV DNA-positive patients and 18 HPV DNA-negative patients were measured using flow cytometry. Fifteen uterine cervix biopsy samples embedded in paraffin were subjected to immunohistochemical analysis for the detection of IL-6+ cells. HR-HPV-positive patients showed increased IL-6 and IL-10 in the ECCs and serum, respectively. Compared with HPV DNA-positive patients, HPV DNA-negative patients had higher levels of IL-6 in ECCs. Patients with multiple infections of HPV had higher levels of IL-6 in their ECCs than those with a single infection. Immunostaining of uterine cervix biopsy samples revealed no differences in IL-6 expression between the different classes of histopathological lesions. However, differences were observed in the expression levels of IL-6 and IL-10 at the systemic and local levels in HR-HPV-positive patients without cervical lesions. Considering the functional characteristics of these cytokines, it can be inferred that such patients are prone to persistent HPV infection.

scholarly journals Molecular characterization of high-risk human papillomavirus (HR-HPV) in women in Lomé, Togo

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Akouélé P. Kuassi-Kpede ◽  
Essolakina Dolou ◽  
Théodora M. Zohoncon ◽  
Ina Marie Angèle Traore ◽  
Gnatoulma Katawa ◽  
...  
Keyword(s):  
Human Papillomavirus ◽  
High Risk ◽  
West Africa ◽  
Public Health Problem ◽  
Hpv Infection ◽  
Cervical Lesions ◽  
Hpv Genotypes ◽  
Precancerous Cervical Lesions ◽  
High Risk Hpv ◽  
First Time

Abstract Background The causative agent of cervical cancer referred to as Human papillomavirus (HPV) remains a real public health problem. Many countries in West Africa, such as Togo have no data on the high-risk HPV (HR-HPV) infection and genotypes distribution. In order to fill the knowledge gap in the field in Togo, the main objective of this study was to determine the prevalence of pre-cancerous lesions of the cervix and HR-HPV genotypes among Togolese women. Methods Samples were collected from 240 women by introducing a swab in the cervix. Then, the screening of precancerous cervical lesions using the visual inspection with acetic acid and lugol (VIA / VIL) was conducted. The HR-HPV genotypes were characterised by real-time multiplex PCR. Results Out of 240 women recruited, 128 (53.3%) were infected by HR-HPV. The most common genotypes were HPV 56 (22.7%), followed by HPV 51 (20.3%), HPV 31 (19.5%), HPV 52 (18.8%) and HPV 35 (17.2%). The least common genotypes were HPV 33 (2.3%) and HPV 16 (2.3%). Among the women, 1.3% (3/240) were positive to VIA/VIL. Conclusion This study allowed HR-HPV genotypes to be characterised for the first time in Lomé, Togo. This will help in mapping the HR-HPV genotypes in West Africa.

scholarly journals Genome-Wide Association Study of Prevalent and Persistent Cervical High-Risk Human Papillomavirus(HPV) Infection

2019 ◽  
Author(s):  
Sally N. Adebamowo ◽  
Adebowale A Adeyemo ◽  
Charles N Rotimi ◽  
Olayinka Olaniyan ◽  
Richard B. Offiong ◽  
...  
Keyword(s):  
Human Papillomavirus ◽  
High Risk ◽  
Association Study ◽  
Genome Wide Association Study ◽  
Principal Component ◽  
Hpv Infection ◽  
Genome Wide Association ◽  
Replication Studies ◽  
Genome Wide

Abstract Background: Genetic factors may influence the susceptibility to high-risk human papillomavirus (hrHPV) infection and persistence. We conducted the first genome-wide association study (GWAS) to identify variants associated with cervical hrHPV infection and persistence. Methods: Participants were 517 Nigerian women evaluated at baseline and 6 months follow-up visits for HPV. HPV was characterized using SPF10/LiPA25. hrHPV infection was positive if at least one carcinogenic HPV genotype was detected in a sample provided at the baseline visit and persistent if at least one carcinogenic HPV genotype was detected in each of the samples provided at the baseline and follow-up visits. Genotyping was done using the Illumina Multi-Ethnic Genotyping Array (MEGA) and imputation was done using the African Genome Resources Haplotype Reference Panel. Association analysis was done under additive genetic models adjusted for age, HIV status and the first principal component (PC) of the genotypes. Results: The mean (±SD) age of the study participants was 38 (±8) years, 48% were HIV negative, 24% were hrHPV positive and 10% had persistent hrHPV infections. The top three variants associated with hrHPV infections were intronic variants clustered in KLF12 (all OR: 7.06, p=1.43 x 10-6). The top variants associated with cervical hrHPV persistence were in DAP(OR: 6.86, p=7.15 x 10-8), NR5A2(OR: 3.65, p=2.03 x 10-7) and MIR365-2(OR: 7.71, p=2.63 x 10-7) gene regions. Conclusions: This exploratory GWAS yielded novel candidate risk loci for cervical hrHPV infection and persistence. The identified loci have biological annotation and functional data supporting their role in hrHPV infection and persistence. Given our limited sample size, larger discovery and replication studies are warranted to further characterize the reported associations.

scholarly journals Detection and estimation of human papillomavirus viral load in patients with cervical lesions

2014 ◽  
Vol 39 (2) ◽  
pp. 86-90 ◽  
Author(s):  
T Rahman ◽  
S Tabassum ◽  
M Jahan ◽  
A Nessa ◽  
Dr Ashrafunnessa
Keyword(s):  
Human Papillomavirus ◽  
Viral Load ◽  
High Risk ◽  
Invasive Cervical Cancer ◽  
Hpv Infection ◽  
Cervical Lesions ◽  
Hpv Dna ◽  
Promising Tool ◽  
High Risk Hpv ◽  
Hpv Viral Load

Human papillomavirus (HPV) high risk genotype infection and HPV viral load influences the development of invasive cervical cancer and cervical intra-epithelial neoplasia (CIN). HPV DNA testing for screening of cervical cancers may play a potential role in its early detection and management. The present study detected HPV DNA and estimated HPV viral load in different types of cervical lesions among Bangladeshi women. Using the Hybrid Capture 2 (HC2) assay, HPV DNA was tested among 68 women between 25-70 years of age. A total of 13 (19.1%) cases were positive for HPV DNA. The highest viral load (501 x 10³ copies/ml) was detected in a patient with invasive carcinoma, while the lowest viral load (105 x 10³ copies/ml) was detected from a case of chronic cervicitis. The mean viral load in CIN I was 119.25 x 10³±12.5 x 10³ copies/ml (range: 110 x 10³ - 137 x 10³) and 208.50 x 10³ ± 0.59 x 10³ copies/ml (range: 139 x 10³-305 x 10³) in CIN II / III. Interestingly, HPV DNA was detected from a patient with normal cytological findings. Our study observed a moderate presence of high-risk HPV genotypes among women with cervical lesions. The HPV viral load varied with the age of the patients and stage of cervical lesions. The HC2 assay is a promising tool for diagnosing high-risk HPV infection especially before cytology tests show any abnormality. DOI: http://dx.doi.org/10.3329/bmrcb.v39i2.19648 Bangladesh Med Res Counc Bull 2013; 39: 86-90

scholarly journals Epidemiological Characteristics and Genotypes of Human Papillomavirus Infection in Mianyang

2021 ◽  
Vol 5 (6) ◽  
pp. 61-67
Author(s):  
Daping Song ◽  
Min Min ◽  
Pingjing Guo ◽  
Xuemei Zhou
Keyword(s):  
High Risk ◽  
Infection Rate ◽  
Age Groups ◽  
Hpv Infection ◽  
Low Risk ◽  
Multiple Infection ◽  
Infection Rates ◽  
High Risk Hpv ◽  
Epidemiological Characteristics ◽  
Risk Infection

Objective: To investigate the epidemiological characteristics and genotypes of human papillomavirus (HPV) infection in Mianyang. Methods: The cervical samples of 27,040 patients, who visited the Department of Gynecology of The Third Hospital of Mianyang from January 2018 to January 2020, were collected. Results: The HPV-positive infection rate was 21.40% (5,787/27,040); the single HPV infection rate was 72.04% (4,169/5,787); the double HPV infection rate was 19.73% (1,142/5,787); the triple and above HPV infection rate was 8.22% (476/5,787); the top five high-risk HPV subtypes with the highest infection rates were HPV52, 16, 58, 53, and 51, while the top five low-risk HPV subtypes with the highest infection rates were HPV81, 42, 43, 6, and 11; there were significant differences in the HPV-positive infection rate, high-risk infection rate, low-risk infection rate, and multiple infection rate among different age groups (P < 0.05). Conclusion: The HPV infection rate in Mianyang was 21.4%, in which the majority of the cases were single infection; the high-risk HPV subtypes were HPV52, 16, 58, 53, and 51; the HPV-positive infection rate, high-risk infection rate, low-risk infection rate, and multiple infection rate were high in the middle but low at both ends in the context of age distribution; the top three age groups with the highest infection rates were 45-49, 40-44, and 30-34.

scholarly journals Current studies on human papillomavirus in Saudi Arabia

2015 ◽  
Vol 9 (06) ◽  
pp. 571-576 ◽  
Author(s):  
Fatimah Saeed Alhamlan ◽  
Ahmed A Al-Qahtani ◽  
Mohammed N Al-Ahdal
Keyword(s):  
Cervical Cancer ◽  
Saudi Arabia ◽  
Human Papillomavirus ◽  
High Risk ◽  
Hpv Infection ◽  
World Health ◽  
Cervical Lesions ◽  
Hpv Types ◽  
Health Organization ◽  
Low Prevalence

Human papillomavirus (HPV) infection is a significant etiological factor and an important prognosticator in cervical cancer. Indeed, researchers worldwide have confirmed these roles for high-risk HVPs in over 70% of cervical cancer cases. According to the World Health Organization, approximately 561,200 new cancer cases (5.2% of all new cancers) are attributed to HPV infection. Over 120 types of HPV are classified further as either low-risk HPV (LR-HPV) or high-risk HPV (HR-HPV) based on their oncological potential of transforming cells. The LR-HPV types cause benign hyperproliferative lesions (i.e. genital warts) while the HR-HPV types are strongly associated with premalignant and malignant cervical lesions. Data on the prevalence of HPV, survival of infected patients, and mortality rate are scarce in Saudi Arabia. The unsubstantiated assumption of a low prevalence of HPV in Saudi Arabia has contributed to limiting HPV research in this conservative country. Therefore, the goal of this review is to shed light on the current HPV research being conducted and the prevalence of HPV in Saudi Arabia.

scholarly journals Research Progress of HPV L1 Capsid Protein in Prediction of Cervical Lesions

2020 ◽  
Vol 4 (6) ◽  
Author(s):  
Jing Cheng ◽  
Lin Xu ◽  
Beibei Liu ◽  
Biao Wang ◽  
Xicui Long ◽  
...  
Keyword(s):  
Cervical Cancer ◽  
Human Papillomavirus ◽  
Capsid Protein ◽  
Hpv Infection ◽  
Research Progress ◽  
Patient Treatment ◽  
Structural Protein ◽  
Cervical Lesions ◽  
Cervical Cells ◽  
Cellular Immune

Cervical cancer is one of the most common malignant gynecological tumors and has the second highest incidence of all malignancies in females. Chronic and persistent infection with High Risk Human Papillomavirus (HR-HPV) is the main cause of cervical cancer. There is a distinct lack of methodology by which to determine whether cervical epithelial dysplasia is cancerous following HPV infection. HPV L1 capsid protein is a major structural protein of human papillomavirus (HPV)?and it is the main target of the local cellular immune response aiming to combat human papillomavirus after HPV infection within cervical cells. Greater understanding of HPV L1 capsid protein and its association with cervical cytology, histopathology, patient age and human papillomavirus viral load has the potential to contribute toward improved the improved diagnosis and management of cervical cancer, providing useful information for gynecological clinicians in the hope of improving patient treatment and quality of life. This article reviews the predictive utility of HPV L1 capsid protein for cervical lesions.

scholarly journals Human papillomavirus as a single infection in pregnant women from Northeastern Mexico: Cross-sectional study

2020 ◽  
Author(s):  
Bernardo Martínez-Leal ◽  
Karla Ivette Álvarez-Banderas1 ◽  
Homero Sánchez-Dávila ◽  
Martha Imelda Dávila-Rodríguez ◽  
Elva Irene Cortés-Gutiérrez
Keyword(s):  
Human Papillomavirus ◽  
Pregnant Women ◽  
Hpv Infection ◽  
Smoking History ◽  
Single Infection ◽  
Multiple Infections ◽  
Control Group ◽  
Cervical Lesions ◽  
Cross Sectional ◽  
Northeastern Mexico

Background: The role of human papillomavirus (HPV) as single or multiple infections in pregnant women would be relevant to determining the time to progression and/or the time to regression of cervical lesions. Objective: In this preliminary study, we determined the prevalence of HPV as single or multiple infections in pregnant women from Northeastern Mexico. Materials and Methods: Samples from 31 pregnant and 62 nonpregnant women were examined between January 2015 and November 2015 at UMAE-23 of the Instituto Mexicano del Seguro Social (IMSS). The samples of cervicovaginal exudate were obtained for HPV DNA detection using the INNO-LiPA test, and HPV infections were analyzed as single or multiple infections. Participants completed a questionnaire on sociodemographic, gynecological, obstetric, and sexual behavior characteristics. Results: The mean age of the pregnant women was 25.7 ± 4.8 yr, with an average time of pregnancy of 6 ± 1 months at the time of the study. With respect to age, parity, smoking history, or oral contraceptive use no statistically significant differences between the two studied groups was observed. The HPV infection was 2.7 times higher in pregnant women (35%) than in the control group (13%). In total, 78% of the pregnant women who were HPV-positive presented with single infections compared with 28% of the nonpregnant women. Conclusion: A higher prevalence of HPV as a single infection was found in this sample of pregnant Mexican women. Follow-up is necessary to evaluate the persistence or regression of the infection. Key words: Papillomavirus infection, Pregnant women, Uterine cervical dysplasia.

scholarly journals Coinfection of High-Risk Human Papillomavirus and Lower Genital Tract Pathogens in the Development of High-Grade Cervical Lesions

2020 ◽  
Vol 2020 ◽  
pp. 1-8
Author(s):  
Hui Zhong ◽  
Yao Tong ◽  
Haifeng Lin ◽  
Xiaodan Mao ◽  
Binhua Dong ◽  
...  
Keyword(s):  
Human Papillomavirus ◽  
High Risk ◽  
Genital Tract ◽  
Hpv Infection ◽  
High Grade ◽  
Infection Rates ◽  
Squamous Intraepithelial Lesions ◽  
Cervical Lesions ◽  
Lower Genital Tract ◽  
Intraepithelial Lesions

Purpose. This study investigated the infection status and relationship between other common lower genital tract infectious pathogens and high-risk human papillomavirus (HR-HPV) in the high-grade cervical lesions. Methods. Overall, 882 patients were enrolled in this retrospective study, of which 339 patients (≥HSIL group) were confirmed with high-grade squamous intraepithelial lesions (HSIL) or cervical squamous cell carcinoma (SCC), while 543 patients (≤LSIL group) were diagnosed with low-grade squamous intraepithelial lesions (LSIL) or normal cervical pathology diagnosis. Cervical swab specimens were tested for HPV, pathogenic bacteria (PB), U. urealyticum (UU), M. hominis (MH), and C. trachomatis (CT) in both groups. Results. The infection rates of HR-HPV, PB, UU (at high density), and CT were higher in the ≥HSIL group than in the ≤LSIL group (P<0.001); however, higher infection rates with MH were not observed (P>0.05). PB, UU, and CT were associated with HR-HPV infection (P<0.001). The PB and UU infection rates in the ≥HSIL group were significantly different from those in the ≤LSIL group, regardless of whether there was an HR-HPV infection at the same time (P<0.05). However, this was not the case for the CT (P>0.05). Furthermore, 259 pathogenic bacterial strains were detected in 882 cases. The difference in the distribution of pathogenic bacterial flora in the different grades of cervical lesions had no statistical significance, which was prioritized over Escherichia coli (P>0.05). Conclusion. PB, UU, and CT infection is associated with susceptibility to HR-HPV, HR-HPV coinfection with these pathogens might increase the risk of high-grade cervical lesions, and PB and UU might be independent risk factors for cervical lesions.

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