scholarly journals In vitro performance in cotton plants with different genetic backgrounds: the case of Gossypium hirsutum in Mexico, and its implications for germplasm conservation

PeerJ ◽  
2019 ◽  
Vol 7 ◽  
pp. e7017 ◽  
Author(s):  
Alejandra Hernández-Terán ◽  
Ana Wegier ◽  
Mariana Benítez ◽  
Rafael Lira ◽  
Tania Gabriela Sosa Fuentes ◽  
...  
Keyword(s):  
Gossypium Hirsutum ◽  
Biodiversity Conservation ◽  
Crop Production ◽  
Statistical Tests ◽  
Germplasm Conservation ◽  
Ex Situ ◽  
Conservation Strategies ◽  
Wild Germplasm ◽  
Wild Cotton

One of the best ex situ conservation strategies for wild germplasm is in vitro conservation of genetic banks. The success of in vitro conservation relies heavily on the micropropagation or performance of the species of interest. In the context of global change, crop production challenges and climate change, we face a reality of intensified crop production strategies, including genetic engineering, which can negatively impact biodiversity conservation. However, the possible consequences of transgene presence for the in vitro performance of populations and its implications for biodiversity conservation are poorly documented. In this study we analyzed experimental evidence of the potential effects of transgene presence on the in vitro performance of Gossypium hirsutum L. populations, representing the Mexican genetic diversity of the species, and reflect on the implications of such presence for ex situ genetic conservation of the natural variation of the species. We followed an experimental in vitro performance approach, in which we included individuals from different wild cotton populations as well as individuals from domesticated populations, in order to differentiate the effects of domestication traits dragged into the wild germplasm pool via gene flow from the effects of transgene presence. We evaluated the in vitro performance of five traits related to plant establishment (N = 300): propagation rate, leaf production rate, height increase rate, microbial growth and root development. Then we conducted statistical tests (PERMANOVA, Wilcoxon post-hoc tests, and NMDS multivariate analyses) to evaluate the differences in the in vitro performance of the studied populations. Although direct causality of the transgenes to observed phenotypes requires strict control of genotypes, the overall results suggest detrimental consequences for the in vitro culture performance of wild cotton populations in the presence of transgenes. This provides experimental, statistically sound evidence to support the implementation of transgene screening of plants to reduce time and economic costs in in vitro establishment, thus contributing to the overarching goal of germplasm conservation for future adaptation.

scholarly journals In vitro propagation and cytological analysis of Sophora mollis Royle: an endangered medicinal shrub

2021 ◽  
Vol 19 (1) ◽  
Author(s):  
Aakriti Bhandari ◽  
Harminder Singh ◽  
Amber Srivastava ◽  
Puneet Kumar ◽  
G. S. Panwar ◽  
...  
Keyword(s):  
Chromosome Number ◽  
Average Length ◽  
Germplasm Conservation ◽  
Basic Chromosome Number ◽  
Shoot Tip ◽  
Ex Situ ◽  
Ms Medium ◽  
Basic Chromosome ◽  
Shoot Tip Explants

Abstract Background Sophora mollis Royle (family Fabaceae, subfamily-Papilionaceae) is a multipurpose legume distributed in plains and foothills of the North-West Himalaya to Nepal and is facing high risk of extinction due to habitat loss and exploitation by the local people for its fuel and fodder values. Therefore, the present study was conducted to standardize a micropropagation protocol for Sophora mollis by using shoot tip explants and to study the meiotic chromosome count in the species. Results Multiple shoots were induced in shoot tip explants of Sophora mollis in Murashige and Skoog medium supplemented with different concentrations of cytokinins alone (BAP, TDZ, and Kinetin) and in combination with varying concentrations of NAA. MS medium supplemented with BAP (8.9 μM) was observed to be the optimal medium for multiple shoot induction and maximum 25.32 shoots per explant was obtained with average length of 4.5 ± 0.8 cm. In vitro developed shoots were transferred onto rooting media supplemented with different concentrations of auxin (IAA, IBA, and NAA). Maximum 86% rooting was observed in half-strength MS medium supplemented with 21.20 μM NAA with an average of 21.26 roots per culture. In vitro raised plantlets were adapted to greenhouse for better acclimatization and 60% plants were successfully transferred to the open environment. Based on the chromosome counts available from the literature and the current study, the species tend to show a basic chromosome number of x = 9. Conclusion The micropropagation protocol standardized can be helpful for the ex situ mass multiplication and germplasm conservation of the endangered species. Moreover, the ex situ conservation approach will be helpful in actively bridging the gap between ex situ and in situ approaches through the reintroduction of species in the wild. The cytological studies revealed the basic chromosome number x = 9 of the species.

scholarly journals Cryopreservation of Woody Crops: The Avocado Case

Plants ◽  
2021 ◽  
Vol 10 (5) ◽  
pp. 934
Author(s):  
Chris O’Brien ◽  
Jayeni Hiti-Bandaralage ◽  
Raquel Folgado ◽  
Alice Hayward ◽  
Sean Lahmeyer ◽  
...  
Keyword(s):  
Tissue Culture ◽  
Ex Situ Conservation ◽  
Plant Genetic Resources ◽  
Ex Situ ◽  
Conservation Strategies ◽  
Recalcitrant Seed ◽  
In Vitro Storage ◽  
Germplasm Collections ◽  
Seed Crops

Recent development and implementation of crop cryopreservation protocols has increased the capacity to maintain recalcitrant seeded germplasm collections via cryopreserved in vitro material. To preserve the greatest possible plant genetic resources globally for future food security and breeding programs, it is essential to integrate in situ and ex situ conservation methods into a cohesive conservation plan. In vitro storage using tissue culture and cryopreservation techniques offers promising complementary tools that can be used to promote this approach. These techniques can be employed for crops difficult or impossible to maintain in seed banks for long-term conservation. This includes woody perennial plants, recalcitrant seed crops or crops with no seeds at all and vegetatively or clonally propagated crops where seeds are not true-to-type. Many of the world’s most important crops for food, nutrition and livelihoods, are vegetatively propagated or have recalcitrant seeds. This review will look at ex situ conservation, namely field repositories and in vitro storage for some of these economically important crops, focusing on conservation strategies for avocado. To date, cultivar-specific multiplication protocols have been established for maintaining multiple avocado cultivars in tissue culture. Cryopreservation of avocado somatic embryos and somatic embryogenesis have been successful. In addition, a shoot-tip cryopreservation protocol has been developed for cryo-storage and regeneration of true-to-type clonal avocado plants.

scholarly journals Application of the MSAP Technique to Evaluate Epigenetic Changes in Plant Conservation

2020 ◽  
Vol 21 (20) ◽  
pp. 7459
Author(s):  
María Elena González-Benito ◽  
Miguel Ángel Ibáñez ◽  
Michela Pirredda ◽  
Sara Mira ◽  
Carmen Martín
Keyword(s):  
Dna Methylation ◽  
In Situ Conservation ◽  
Plant Conservation ◽  
Ex Situ ◽  
Conservation Strategies ◽  
Regenerated Plants ◽  
Before And After ◽  
Methylation Patterns

Epigenetic variation, and particularly DNA methylation, is involved in plasticity and responses to changes in the environment. Conservation biology studies have focused on the measurement of this variation to establish demographic parameters, diversity levels and population structure to design the appropriate conservation strategies. However, in ex situ conservation approaches, the main objective is to guarantee the characteristics of the conserved material (phenotype and epi-genetic). We review the use of the Methylation Sensitive Amplified Polymorphism (MSAP) technique to detect changes in the DNA methylation patterns of plant material conserved by the main ex situ plant conservation methods: seed banks, in vitro slow growth and cryopreservation. Comparison of DNA methylation patterns before and after conservation is a useful tool to check the fidelity of the regenerated plants, and, at the same time, may be related with other genetic variations that might appear during the conservation process (i.e., somaclonal variation). Analyses of MSAP profiles can be useful in the management of ex situ plant conservation but differs in the approach used in the in situ conservation. Likewise, an easy-to-use methodology is necessary for a rapid interpretation of data, in order to be readily implemented by conservation managers.

scholarly journals Propagation of Juniper Species by Plant Tissue Culture: A Mini-Review

Forests ◽  
2019 ◽  
Vol 10 (11) ◽  
pp. 1028 ◽  
Author(s):  
Hazubska-Przybył
Keyword(s):  
In Vitro Culture ◽  
Protective Action ◽  
Ex Situ ◽  
Conservation Strategies ◽  
Culture Techniques ◽  
Effective Strategies ◽  
Dry Climates ◽  
Coniferous Tree Species ◽  
Seed Propagation

The genus Juniperus (of the Cupressaceae family) is the second most prevalent group of conifers on Earth. Juniper species are widely dispersed in the Northern Hemisphere, in Europe and Asia, and in Africa and Central America. Juniper species are resistant to dry climates and can adapt to difficult environmental conditions. Most juniper species are important in both ecological and economic terms. However, today, many forests in which junipers occur are being reduced in size due to both natural causes (fires, for example) and human activity (uncontrolled exploitation of forests, etc.). Also, climate changes may have adversely affected the range of populations of different juniper species. For this reason, some juniper species are now categorized as rare or endangered, and require immediate protective action. Therefore, there is an urgent need to develop effective strategies for ex situ conservation, including reliable procedures for Juniperus sp. reproduction for future reintroduction and restoration programs. The conservation strategies used until now with traditional forestry techniques (seed propagation, rooted cuttings, grafting) have not been satisfactory in many cases. Thus, increasing attention is being paid to the possibilities offered by in vitro culture technology, which enables the conservation and mass clonal propagation of different coniferous tree species. In this mini-review, we summarize the current state of knowledge regarding the use of various methods of the propagation of selected Juniperus species, with a particular emphasis on in vitro culture techniques.

scholarly journals Preserving grapevine variety Fioletoviy Ranniy in the collection in vitro

2021 ◽  
Vol 273 ◽  
pp. 01007
Author(s):  
Valentina Puzirnova ◽  
Natalia Doroshenko
Keyword(s):  
Biodiversity Conservation ◽  
Sustainable Management ◽  
Future Generations ◽  
Ex Situ ◽  
Grapevine Variety ◽  
The World ◽  
History Of ◽  
Slow Growing

The paper is devoted to the problem of plant biodiversity conservation. This problem is acute all over the world. Lower Don Region has a centuries old history of viticulture and winemaking. There are many valuable vine varieties which are worthy of preservation for future generations. Classical methods no longer cope with this task. Applying advances of biotechnology in addition to traditional methods of ex situ and in situ biodiversity conservation allows sustainable management of genetic resources. This article summarizes the study of methods for creation slow growing collection for grapevine variety Fioletoviy Ranniy. Keeping plants in a slow-growing collection is one of the best ways to preserve biodiversity. This study analyzed the effect of various media compounds on vigor of vine in order to elongate the time between replantings.

scholarly journals Initial stages of Gymnadenia conopsea (Orchidaceae) morphogenesis in in vitro culture

2020 ◽  
Vol 24 ◽  
pp. 00059
Author(s):  
Alexandra Nabieva ◽  
Elena Zhmud ◽  
Yulianna Zaytseva
Keyword(s):  
Environmental Changes ◽  
Germplasm Conservation ◽  
West Siberia ◽  
Habitat Destruction ◽  
Ex Situ ◽  
Naphthaleneacetic Acid ◽  
Gymnadenia Conopsea ◽  
Initial Stage

In the West Siberia there is very little data on the biology of cenopopulations of Gymnadenia conopsea. Habitat destruction and environmental changes, which potentially affected species long-term viability, led to G. conopsea cenopopulations fragmentation and isolation. A detailed study of the morphology variability of the Fragrant orchid representatives was carried out in the cenopopulation in Novosibirsk region. Our results indicate that asymbiotic germination of G. conopsea seeds is difficult to achieve and the species has serious conservation issues. It is the first report when the introduction of G. conopsea in tissue culture was undertaken as the initial stage of germplasm conservation of Siberian cenopopulation. Three modified nutrient media with different growth additives were tested to promote protocorm and seedling formation. The advanced G. conopsea seedlings establishment was obtained in 1/3 Murashige and Skoog (MS) medium, supplemented by 1.0 mgl−1 2-isopentenyladenine (2iP), 0.1 mgl−1 1-Naphthaleneacetic acid (NAA) and 10% coconut water. This study allowed establishing a reliable and reproducible system for the G. conopsea maintenance and conservation ex situ.

scholarly journals In-Situ and Ex-Situ Biodiversity Conservation in Ecuador: A Review of Policies, Actions and Challenges

Diversity ◽  
2020 ◽  
Vol 12 (8) ◽  
pp. 315 ◽  
Author(s):  
Carlos Mestanza-Ramón ◽  
Sujan M. Henkanaththegedara ◽  
Paola Vásconez Duchicela ◽  
Yadira Vargas Tierras ◽  
Maritza Sánchez Capa ◽  
...  
Keyword(s):  
Protected Areas ◽  
Biodiversity Conservation ◽  
Ex Situ Conservation ◽  
In Situ Conservation ◽  
Well Being ◽  
Ex Situ ◽  
Policy Framework ◽  
Conservation Strategies ◽  
Small Scale

Biodiversity is vital for the stability of the planet; its ecosystem services provide essential elements for our survival and well-being. This review analyzes the national biodiversity policies and describes the main strategies for biodiversity conservation in Ecuador, one of the “mega-diverse” countries in the world with the highest species density. It deepens an analysis of in-situ and ex-situ conservation processes. Ecuador has six clear policies for biodiversity conservation. These policies strengthen biodiversity conservation through mechanisms that improve the well-being of wildlife by ensuring human, wildlife and ecosystem health. It promotes actions for the welfare of wildlife, through technical, administrative and legal tools. The National System of Protected Areas, with 60 protected areas, is the most effective in-situ conservation instrument at the country level. Several ex-situ conservation and management means for the conservation of wild species are being utilized, including nurseries, botanical gardens, zoos, germplasm banks, aquariums, species reproduction and rehabilitation centers. Ecuador is making slow progress on ex-situ conservation despite the availability of a sound policy framework, possibly due to financial, infrastructural, and/or technological challenges, and knowledge gaps. We propose fostering international research collaborations and establishing fully funded small-scale captive breeding programs at zoos, aquariums and university research facilities to help recovery of at-risk species of reptiles, amphibians, fish and species beyond Galapagos region. We recommend utilizing citizen science programs to fill the gaps of biodiversity information and increasing efforts to revive the ex-situ conservation strategies in protecting the unique biodiversity of Ecuador.

scholarly journals In Vitro and Cryobiotechnology Approaches to Safeguard Lupinus rivularis Douglas ex Lindl., an Endangered Plant in Canada

Agronomy ◽  
2020 ◽  
Vol 11 (1) ◽  
pp. 37
Author(s):  
Elena V. Popova ◽  
Mukund R. Shukla ◽  
Terry McIntosh ◽  
Praveen K. Saxena
Keyword(s):  
Seed Germination ◽  
Endangered Species ◽  
In Situ Conservation ◽  
Germplasm Conservation ◽  
Basal Medium ◽  
Multiple Shoot ◽  
Shoot Tips ◽  
Ex Situ ◽  
Natural Habitats

Conservation of threatened flora in genetic collections ex situ using in vitro culture and cryopreservation is receiving an increasing recognition as a complementary strategy to in situ conservation in natural habitats. The present study is focused on an integrated approach which involves conservation and propagation, emphasizing the usefulness of cryopreservation techniques for germplasm conservation of streambank lupine (Lupinus rivularis Douglas ex Lindl.), an endangered species in Canada. This included in vitro seed germination on Murashige and Skoog basal medium supplemented with 1 µM thidiazuron to induce multiple shoot formation, micropropagation on a medium with 5 µM benzylaminopurine, and in vitro rooting on medium with 20.0 µM indole-3-butyric acid. Cryopreservation of seeds and shoot tips of in vitro grown plants was successful with over 60% seed germination and 62% regrowth of cryopreserved shoot tips, respectively. Plants developed from cryopreserved seeds had chlorophyll contents in leaves and the growth characteristics including the development of inflorescence, similar to plants raised from non-cryopreserved seeds. These results provide further evidence that the combination of micropropagation with cryopreservation of seeds and vegetative parts may effectively facilitate long-term preservation of L. rivularis and other endangered species.

scholarly journals In Vitro Propagation, Genetic Assessment, and Medium-Term Conservation of the Coastal Endangered Species Tetraclinis Articulata (Vahl) Masters (Cupressaceae) from Adult Trees

Plants ◽  
2022 ◽  
Vol 11 (2) ◽  
pp. 187
Author(s):  
Jorge Juan-Vicedo ◽  
Francisco Serrano-Martínez ◽  
Miriam Cano-Castillo ◽  
José Luis Casas
Keyword(s):  
Basal Medium ◽  
Ex Situ ◽  
Conservation Strategies ◽  
Peat Moss ◽  
Naphthaleneacetic Acid ◽  
Arid Environments ◽  
Multiplication Rate ◽  
Tetraclinis Articulata ◽  
Adult Trees

Tetraclinis articulata (Vahl) Masters is an endangered tree growing in coastal and arid environments that is widely exploited by the timber and resin industry, among other applications. In this context, the use of in vitro techniques is highly encouraged for its propagation. We present a protocol for micropropagation using twigs from adult trees as a source of explants. The Schenk and Hildebrandt basal medium (SH) supplemented with 30 g L−1 sucrose, 6.5 g L−1 plant agar, 4.0 mg L−1 6-benzyladenine (BA), and 0.05 mg L−1 1-naphthaleneacetic acid (NAA) provided the optimum multiplication rate (90.48 ± 9.52 explants with basal shoots and 2.58 ± 0.29 basal shoots per explant). Application of activated charcoal (AC) or ½ Knop solution in a liquid overlay produced significantly longer shoots. Supplementation of solid media with indole-3-butyric acid (IBA) or NAA gave low rooting percentages (<17%). Addition of 0.9 g L−1 AC improved rooting (40%) but rooting performance was optimal (66.7%) after a pulse treatment consisting of 4 h immersion in liquid SH medium without growth regulators, followed by 8 weeks of cultivation. Rooted microplants were successfully acclimatized (93.33%) in a peat moss and vermiculite mixture (1:1 v/v ratio). The genetic stability of the in vitro regenerated plantlets was confirmed using the randomly amplified polymorphic DNA (RAPD) technique. Explant survival and growth remained higher than 90% after 28 weeks of cold storage at both 4 °C and 10 °C. The protocol presented here allows for largescale T. articulata production and could be applied for both ex situ conservation strategies and industrial purposes.

scholarly journals Genetic variability and population structure of Ethiopian chickpea (Cicer arietinum L.) germplasm

PLoS ONE ◽  
2021 ◽  
Vol 16 (11) ◽  
pp. e0260651
Author(s):  
Sintayehu Admas ◽  
Kassahun Tesfaye ◽  
Teklehaimanot Haileselassie ◽  
Eleni Shiferaw ◽  
K. Colton Flynn
Keyword(s):  
Genetic Diversity ◽  
Population Structure ◽  
Ssr Markers ◽  
Polymorphic Information Content ◽  
Germplasm Conservation ◽  
Genetic Distances ◽  
Geographic Region ◽  
Ex Situ ◽  
Conservation Strategies ◽  
Breeding Programs

Evaluation of the genetic diversity and an understanding of the genetic structure and relationships of chickpea genotypes are valuable to design efficient germplasm conservation strategies and crop breeding programs. Information is limited, in these regards, for Ethiopian chickpea germplasms. Therefore, the present study was carried out to estimate the genetic diversity, population structure, and relationships of 152 chickpea genotypes using simple sequence repeats (SSR) markers. Twenty three SSR markers exhibited polymorphism producing a total of 133 alleles, with a mean of 5.8 alleles per locus. Analyses utilizing various genetic-based statistics included pairwise population Nei’s genetic distance, heterozygosity, Shannon’s information index, polymorphic information content, and percent polymorphism. These analyses exemplified the existence of high genetic variation within and among chickpea genotypes. The 152 genotypes were divided into two major clusters based on Nei’s genetic distances. The exotic genotypes were grouped in one cluster exclusively showing that these genotypes are distinct to Ethiopian genotypes, while the patterns of clustering of Ethiopian chickpea genotypes based on their geographic region were not consistent because of the seed exchange across regions. Model-based population structure clustering identified two discrete populations. These finding provides useful insight for chickpea collections and ex-situ conservation and national breeding programs for widening the genetic base of chickpea.

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