Instantaneous Growth Rate of Freshwater Nannochloropsis spp. at Different Salinity Levels

Author(s):  
Lady Gem H. Maquirang ◽  
Romeo D. Caturao ◽  
Jessy H. Maquirang

A study was conducted to determine the instantaneous growth rate of freshwater Nannochloropsis spp. at five different salinity levels (T1, 0 ppt - control; T2, 5 ppt; T3, 10 ppt; T 4, 15 ppt; and T5, 20 ppt) and were arranged in a Complete Randomized Design (CRD). Nannochloropsis spp. was cultured in fifteen 500 ml plastic experimental bottles based on the assigned treatment for eleven days. Initial sampling was done during the start of the experiment, and everyday thereafter. Cell density was counted using haemacytometer and viewed under the microscope. Results showed that the highest cell density and instantaneous growth rate of freshwater Nannochloropsis spp. was obtained in Treatment 3 with 18,510,000.00 cells/ml and16.00±0.36 cells/ml, respectively, while Treatment 5 had the lowest cell density and growth rate (10,040,833.33 cells/ml; 15.38±0.46 cells/ml).One-way ANOVA and Duncan’s Multiple Range Test revealed there was a significant difference (p<0.05) on the days of culture (days 3, 7 and 8) among treatments. Results for both cell density (cells/ml) and instantaneous growth rate (K) proved that freshwater Nannochloropsis spp. can tolerate a wider range of salinity from 5 ppt to 15 ppt. However, further studies should be conducted at a wider range of salinity levels and other variables such as nitrogen levels and light intensity to have a complete picture of the reproductive biology of this species. Keywords - Aquatic Ecology, instantaneous growth rate, freshwater Nannochloropsis spp., salinity levels, experimental design, Philippines, Asia

2019 ◽  
Vol 6 (1) ◽  
pp. 55
Author(s):  
Ongky Wijaya, Boedi Setya Rahardja, Prayogo

Abstract Increasing consumption of catfish and dairy products encourage increased domestic production of catfish. During the period of 5 years (2005-2009), catfish production has been increased significantly with an average annual growth reached 32%. In 2008 production reached 114.371 tonnes and in 2009 production increased by almost 75% to about 200 thousand tons. Aquaponic is a bio-integration that links recirculating aquaculture principles to the production of crops / vegetables hydroponically (Diver, 2006). Aquaponic technology has proven to successfully produce an optimal fish on less land and limited water resources, including in urban areas (Ahmad, 2007). This study aims to find out the influences of catfish stocking density difference on the growth rate and survival rate of catfish on aquaponic. The experimental design used was completely randomized design (CRD). Analysis of the data processed using Analysis of Variance (ANOVA) to know whether there is influance the growth rate and survival rate between the treatment given. If there are significantly difference then proceed by Duncan's Multiple Range Test. Based on the research obtained the result that there are significant differences in the survival rate (p> 0.01) and there is a significant difference to the rate of growth (p> 0,01). From the results of statistical tests that have been done show that the highest survival rate is in treatment 4 (87.5333%) and the highest growth rate is in treatment 4 (30.5333%). The lowest Survival found in treatment 1 (53.134%). Lowest growth rate found in treatment 1 (16.6%).


2017 ◽  
Vol 1 (1) ◽  
Author(s):  
Ayu Herdianti Primashita ◽  
Boedi Setya Rahardja ◽  
Prayogo .

Catfish (Clarias sp.) is a freshwater fish that is widely cultivated because it is a leading commodity. Increased production of catfish resulted in extra area of cultivated land and water use. The technology suitable to be applied is aquaponic system. In addition to saving land and water use, Aquaponic is also improving business efficiency through the utilization of nutrients from food remains and fish metabolism. Water quality is an important factor for the success of cultivation. Maintenance catfish with the addition of probiotics in aquaponic system can be a solution for maintaining water quality, because it contains bacteria that can increase nitrite to nitrate changes that can be utilized for growth of plants and do not poison the fish. The purpose of this study was to determine the effect of different probiotics in the aquaponic system towards growth rate and survival rate of catfish. The method that used in this research is experimental with completely randomized design (four  treat and five repeated) are P0 (control), P1 (probiotic A), P2 (probiotic B) and P3 (probiotic C). Analysis of data processed using Analysis of Variance. If there are significant differences then continued Duncan's Multiple Range Test. The results showed that the giving of probiotics in the aquaponic system is significantly different (p <0.05). The final conclusion is that the addition of probiotics in  aquaponic system affect the growth rate and survival rate of catfish. The highest specific growth rate (0.025%/ day) and the highest survival rate (77.8%) contained in the P2 treatment. The lowest specific growth rate (0.019% /day) and the lowest survival rate (64.4%) contained in P0 treatment (control). Keywords : Aquaponic, Catfish, Probiotic, Growth Rate, Survival Rate, Clarias sp.


1980 ◽  
Vol 37 (8) ◽  
pp. 1254-1265 ◽  
Author(s):  
Ora E. Johannsson

Relationships between feeding rate, temperature, body size, and time of day were investigated and collated with seasonal assimilation efficiencies of in situ chironomids to construct a picture of chironomid energetics over the year. Temperature controlled feeding rate, and larval size and time of day exerted no effect. The temperature optimum plateaued between 22.0 and 24.5 °C, decreasing logarithmically outside this range. Both assimilation efficiency (AE) and organic content of the food varied seasonally, but were not correlated. Periods of high AE corresponded with the latter part of Melosira or Melosira–Stephanodiscus blooms, while periods of low AE occurred between diatom blooms and during the late summer blue-green bloom. Consequently, growth fluctuated throughout the year, being a result of the interaction of temperature and AE. Anabolic processes dominated at AE greater than 3.5%. Maximum net growth efficiency (NGE) was attained when AE ≥ 6% and temperature ≥ 15 °C. NGE was more sensitive to changes in AE than temperature. Instantaneous growth rate, however, was more sensitive to changes in temperature. Daily ingestion (mg) exceeded body weight by as much as 460–480%, which equals the sediment-processing capacity of freshwater detritivores.Key words: chironomid, feeding rate, assimilation efficiency, instantaneous growth rate, net growth efficiency, algal community, Bay of Quinte


2016 ◽  
Vol 31 (1) ◽  
pp. 33
Author(s):  
Sri Hartati ◽  
Agus Budiyono ◽  
Ongko Cahyono

<p><em>Indonesia has a high diversity of species of orchids. One of them is the Dendrobium orchid. Orchid seeds do not have endosperm should be grown in a medium that has enough nutrients. Orchid propagation in vitro is strongly influenced by the composition of the medium used. Research through experiments aimed at studying the effect of NAA, BAP, NAA and BAP combination of the subculture plantlets growth of  </em><em>hybrids</em><em> orchid Dendrobium biggibum X liniale in Vacin Went media. Research conducted at the Laboratory of Tissue Culture Center for Plant Conservation Bogor Botanical Gardens. </em><em>The experimental design used was completely randomized design (CRD) with two factors. The first factor was the concentration of Naphthalene Acetic Acid /NAA ( 0 ppm, 1 ppm, 3 ppm and 5 ppm). The second factor  was the type media of </em><em>Benzyl Amino Purine</em><em> /BAP ( 0 ppm, 1 ppm, 3 ppm and 5 ppm). </em><em>Each treatment was replicated eight times. Analysis of the data by F test level 5% and if there is a significant difference continued with Duncan Multiple (Duncan's Multiple Range Test) level of 5%. The result showed that </em><em>the addition  </em><em>of NAA 3 ppm </em><em>as much as 4,96 cm </em><em> and BAP 3 ppm </em><em>as much as 4,41 cm </em><em> give a significant effect on the increase of high plantlets</em><em> </em><em>and  so NAA 3 ppm </em><em>as much as 5,76 cm </em><em> effect  on  roots length , but did not significantly effect the number of leaves and roots.</em></p>


Author(s):  
B. L Olajiire-Ajayi

The high cost of purchasing inorganic fertilizer and its impacts on the soil and the environment remains a major concern in environmental management. Consequently, there is the need to explore other ways to enhance soil fertility. The study investigated the effects of Bambusa vulgaris Schrad. and Gliricidia sepium (Jacq) charcoal powders on the growth of Parkia biglobosa (Jacq). Benth seedlings. The experiment was laid out in a Completely Randomized Design (CRD) with 13 treatments and 9 replicates each. Seeds were sowed directly into polyethene pots while the various treatments were added a week after sowing seeds. Watering was done daily while weeding was done periodically. Growth parameters of seedling height (cm), stem diameter (mm) and leaf count were assessed weekly for 16weeks. Data collected was subjected to Analysis of Variance (ANOVA) at 5% level of significance while the means were separated using Duncan Multiple Range Test (DMRT). The result obtained showed that 15g of Gliricidia sepium charcoal powder + 2kg of topsoil) performed best in height and leaf production with values of 15.85cm and 20.58 respectively.5g of Bambusa vulgaris charcoal powder + 2kg of topsoil) performed best with the value of 0.41mm for stem diameter.2kg of topsoil had the least performance for all parameter assessed. The ANOVA result showed that there was significant difference at P>0.5 among the treatments at 5% level of probability in terms of plant height, stem diameter, leaf production. The study concludes that the addition of charcoalas soil additive enhanced growth of Parkia biglobosa seedling at nursery stage.


2020 ◽  
Vol 5 (3) ◽  
pp. 199
Author(s):  
Dedy Kurnianto ◽  
Lisa Fajar Indriana ◽  
Abdul Wahab ◽  
Syafriyadi Hafid ◽  
Balkam Fadlan Badi

<p><strong>Growth and Survival of Sandfish <span style="text-decoration: underline;">Holothuriascabra</span> Juveniles </strong><strong>Grown </strong><strong>with and without Seaweed <span style="text-decoration: underline;">Gracilaria</span> sp</strong><strong>.,</strong><strong> using Floating and Fixed Hapas in </strong><strong>Earthen </strong><strong>Pond</strong><strong>. </strong><span style="text-decoration: underline;">Holothuriascabra</span> known as sandfish have long been used for pharmaceutical, cosmetic, and food industries.Juveniles production is one of the ways to address the problem of raw material availability due to declining sandfish population in the wild. This research aimed to determine growth performance and survival of sandfish juveniles reared with and without seaweed <span style="text-decoration: underline;">Gracilaria</span> sp.  using floating and fixed hapas in earthen pond. The research was conducted from May to September 2014. A completely randomized design was used to arrange 12 hapas of four treatments groups with three replicates including floating hapas without <span style="text-decoration: underline;">G</span><span style="text-decoration: underline;">racilaria</span> sp. (ATR), floating hapas with <span style="text-decoration: underline;">G</span><span style="text-decoration: underline;">racilaria</span>sp. (ADR), fixed hapas without <span style="text-decoration: underline;">G</span><span style="text-decoration: underline;">racilaria</span> sp. (TTR) and fixed hapas with <span style="text-decoration: underline;">G</span><span style="text-decoration: underline;">racilaria</span> sp. (TDR). Some parameters was observed includingweight increment, Growth Rate (GR),Specific Growth Rate(SGR), Biomass, Survival, and Water Quality. The result showed that GR and SGR were not significantlydifferent among treatments (P&gt;0.05). Survial and Biomass showed significant difference among treatments (P &lt;0.05). ATR has the highest GR, SGR, survival, dan biomass whereas TDR has the lowest GR, SGR, survival, dan biomass. Water quality parameters were in optimal range for sandfish rearing. Added<span style="text-decoration: underline;">G</span><span style="text-decoration: underline;">racilaria</span> sp.tend to inhibitGR, SGR, survival, dan biomass.<strong></strong></p>


2019 ◽  
Vol 7 (2) ◽  
pp. 365
Author(s):  
Julio David ◽  
Sartje Lantu ◽  
Henneke Pangkey ◽  
Lawrence J.L. Lumingas ◽  
Jeffrie F. Mokolensang ◽  
...  

This study aims to examine the fish silage for total gain weight, growth rate, and the specific growth rate of common carp (Cyprinus carpio L.), which is cultivated in 12 nets (1x0,5x1m), with the density of 10 fish (3-5 cm) on each net. The experimental design used was a completely randomized design (CRD) with 4 treatments and 3 replications; treatment A with 10% fish silage, treatment B with fish silage 20%, treatment C with 30% fish silage and treatment D were commercial pellet. The ANOVA test showed that the treatment effect among feed A, B, C, and D tested did not give a significant difference to total gain weight, growth rate and the specific growth of carp fry.Keyword: common carp, fish silage, fish feed, growth ABSTRAKPenelitian ini bertujuan untuk menelaah pemberian silase ikan bagi pertumbuhan mutlak, pertumbuhan nisbi, dan pertumbuhan harian ikan mas (Cyprinus carpio L.), yang dipelihara dalam petak-petak jaring berukuran 1x 0,5 x 1 m, dengan padat penebaran 10 ekor ikan berukuran  3-5 cm. Rancangan percobaan yang digunakan adalah rancangan acak lengkap (RAL) dengan 4 perlakuan dan 3 ulangan; perlakuan A pelet berkomposisi silase ikan 10%, perlakuan B pelet berkomposisi silase ikan 20 %, perlakuan C pelet berkomposisi silase ikan 30 % dan perlakuan D adalah pelet komersil. Uji ANOVA menunjukkan bahwa pengaruh perlakuan di antara pakan A, B, C, dan D yang diujicobakan tidak memberikan perbedaan yang nyata terhadap pertumbuhan mutlak, pertumbuhan nisbi dan pertumbuhan harian benih ikan mas.Kata kunci : ikan mas, silase ikan, pakan ikan, pertumbuhan


2019 ◽  
Vol 6 (1) ◽  
pp. 49
Author(s):  
Muhammad Arief, Nur Fitriani, Sri Subekti

Abstract Freshwater culture commodity such as sangkuriang catfish (Clarias sp.) has high sufficient demand. One way to fullfil necessity of sangkuriang catfis demand is accelerate the growth. The aim of this research is to determine the present effect of different probiotics on commercial feed towards growth and feed efficiency of sangkuriang catfish. The research using experimental method, using Completely Randomized Design (CRD). Sangkuriang catfish kept in 30 days with four treatments and five replications, that is P1 (control), P2 (5% probiotics A), P3 (5% probiotics B), and P4 (5% probiotics C). The obtained data were processed by Analysis of Variance (ANOVA) and followed by Duncan Multiple Range Test if there was significant data. The result showed that different probiotics giving significant effect to growth rate and feed efficiency (p<0,05). The best sangkuriang catfish (Clarias sp.) growth rate is P2 treatment 2,88% ± 0,38, and the lowest is P1 treatment at 1,73% ± 0,7. The best feed efficiency is P2 treatment at 54,69% ± 9,67 and the lowest is P1 treatment at 30,27% ± 12,65


2019 ◽  
Vol 7 (2) ◽  
pp. 199
Author(s):  
Novan Agil Permana ◽  
Yudi Cahyoko ◽  
Muhammad Arief

Abstract Currently, African catfish is developing quite rapidly, because African catfish is one of the important commodities in the fishery. Efforts to increase the production of African catfish is to improve the quality of feed. The purpose of this study was to determine the effect of the use of shark waste mill on the growth rate, feed efficiency and survival rate of African catfish fishing. The research method was experimental with completely randomized design (CRD). The research consisted of five treatments and four replications. The treatments used were: (A) shark waste mill at 0% and fish mill by 50%, (B) 5% shark waste mill and 45% fish mill, (C) 10% waste shark mill and 40% fish mill, (D) 15% waste shark mill and fish mill and 35% (E) 20% shark waste mill and 30% fish mill. The main parameters measured were growth, feed efficiency and survival rate. Supporting parameters measured were water quality. Analysis of the data using analysis of variance (ANOVA) and to determine the differences between treatments tested by Duncan Multiple Range Test. The results showed that the giving of shark waste mil on ration had highly different effects on growth rate and feed eficiency of african catfish. The feeding of waste shark mill in ration had no significantly different effect on Survival Rate (SR). The best dose of shark waste mill is treatment C (10%) with growth rate (1,26%/day), feed eficiency (38,64%) and survival rate (55%).


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