Background-The aim of our study is to determine the drinking water quality monitoring and assessment of safe water
supply in tertiary care hospital. Water samples which are collected from different sites of Adesh Medical College and
Hospital, Shahabad, Haryana were processed.
Material and methods- Most Probable Number (MPN) test was done to detect the coliform bacteria in drinking water, samples were collected
from different sites of AMCH, bacterial isolate was identied by culture, colony morphology, Gram's staining and biochemical characterization
of bacteria.
Results- Out of 40 samples of drinking water 7(17.5%) were positive in which coliform bacteria were present, while 33(82.5%) were negative. In
which 1(14.2%) of the total water samples were contaminated with mix growth organism, 1(14.2%) harbored Klebsiella sp., while 5(87.5%)
among 7 water samples were highly contaminated with E.coli species. Out of 7 positive samples, 4(57.1%) water sample were satisfactory,
1(14.2%) were unsatisfactory sample and 2 (28.5%) were suspicious samples.
Conclusion- On the basis of the results obtained, the quality analysis revealed that most of water samples were satisfactory and were not
contaminated with coliforms and various pathogenic bacteria. The water cooler samples showing positive results have E.coli while less of
Klebsiella species. Therefore, we suggest that all the water sources of drinking water should be properly sanitized, continuously monitored and
bacterial load should be estimated on daily basis.
Bacteriological Analysis of Water Samples from Different Points in a Tertiary Care Hospital