scholarly journals Modeling the Antimicrobial Resistance of Enterobacteria Responsible for Urinary Tract Infections in Benin: Another Way to Control Antimicrobial Resistance

2020 ◽  
pp. 48-61
Author(s):  
Victorien Dougnon ◽  
Phénix Assogba ◽  
Jean-Pierre Gnimatin ◽  
Jerrold Agbankpé ◽  
Hornel Koudokpon ◽  
...  
Keyword(s):  
Mathematical Modeling ◽  
Developing Countries ◽  
Urinary Tract ◽  
Antimicrobial Resistance ◽  
Urinary Tract Infections ◽  
Urine Samples ◽  
Public Health Issue ◽  
Beta Lactamase ◽  
Deterministic Models ◽  
Tract Infections

Background: Infectious diseases are serious public health issue both in developing countries and industrialized nations. In developing countries, they are the main cause of high mortality rates. In the second group, existing resistance strains to antibiotics is developing and growing at an alarming rate. The purpose of this study was to produce data of national interest to implement sustainable control program against the spread of antimicrobial resistance strains in Benin. Methods: One hundred and ninety (190) urine samples were collected in selected hospitals in Benin from patients with urinary tract infection. After getting the informed consent from the patients, samples collections were performed under aseptic conditions and cultured for further analysis in the laboratory. The resistance profile of the bacterial strains was established. The search for beta-lactamase production by the isolates was performed using the synergy test for amoxicillin/clavulanic acid and cephalosporins. Mathematical modeling for predicting the development of resistance of the strains by the year 2024 was carried out employing the compartmental deterministic models. Results: Two hundred and thirty (230) strains were identified from the urine samples. Male individuals were the most affected by urinary tract infections. Individuals between the ages of 21-30 were predominantly infected. E. coli was the most isolated species (32.43%) in the urine samples, followed by K. pneumoniae (26.85%) and E. cloacae (25.92%). The susceptibility testing of isolates showed a high resistance to amoxicillin (91.82%). Whereas the lowest resistance was to imipenem (2%). The beta-lactamase was produced by 24.03% of the strains. Escherichia coli (32.43%) was the most productive of broad spectrum beta-lactamase, followed by K. pneumoniae (31.03%). The mathematical modeling revealed a rampant rise in resistance development of the strains to the tested antibiotics. Conclusions: These results provide important data for developing new preventive strategies against the evolution of bacterial resistance to antibiotics. It therefore, further deserves a constructive advocacy so that more actions are taken against the rampant spread of antimicrobial resistance strains in our health facilities as well as in the communities.

Modeling the antimicrobial resistance of enterobacteria responsible for Urinary Tract Infections in Benin: Another way to control Antimicrobial Resistance

2020 ◽  
Author(s):  
DOUGNON Victorien ◽  
ASSOGBA Phenix ◽  
JIBRIL Mohammed ◽  
GNIMATIN Jean-Pierre ◽  
AGBANKPE Jerrold ◽  
...  
Keyword(s):  
Mathematical Modeling ◽  
Developing Countries ◽  
Urinary Tract ◽  
Antimicrobial Resistance ◽  
Urinary Tract Infections ◽  
Urine Samples ◽  
Public Health Issue ◽  
Beta Lactamase ◽  
Deterministic Models ◽  
Tract Infections

Abstract Background: Infectious diseases are serious public health issue both in developing countries and industrialized nations. In developing countries, they are the main cause of high mortality rates. In the second group, existing resistance strains to antibiotics is developing and growing at an alarming rate. The purpose of this study was to produce data of national interest to implement sustainable control program against the spread of antimicrobial resistance strains in Benin. Methods: One hundred and ninety (190) urine samples were collected in selected hospitals in Benin from patients with urinary tract infection. After getting the informed consent from the patients, samples collections were performed under aseptic conditions and cultured for further analysis in the laboratory. The resistance profile of the bacterial strains was established. The search for beta-lactamase production by the isolates was performed using the synergy test for amoxicillin/clavulanic acid and cephalosporins. Mathematical modeling for predicting the development of resistance of the strains by the year 2024 was carried out employing the compartmental deterministic models. Results: Two hundred and thirty (230) strains were identified from the urine samples. Male individuals were the most affected by urinary tract infections. Individuals between the ages of 21-30 were predominantly infected. E. coli was the most isolated species (32.43%) in the urine samples, followed by K. pneumoniae (26.85%) and E. cloacae (25.92%). The susceptibility testing of isolates showed a high resistance to amoxicillin (91.82%). Whereas the lowest resistance was to imipenem (2%). The beta-lactamase was produced by 24.03% of the strains. Escherichia coli (32.43%) was the most productive of broad spectrum beta-lactamase, followed by K. pneumoniae (31.03%). The mathematical modeling revealed a rampant rise in resistance development of the strains to the tested antibiotics. Conclusions: These results provide important data for developing new preventive strategies against the evolution of bacterial resistance to antibiotics. It therefore, further deserves a constructive advocacy so that more actions are taken against the rampant spread of antimicrobial resistance strains in our health facilities as well as in the communities.

scholarly journals Modeling the antimicrobial resistance of enterobacteria responsible for Urinary Tract Infections in Benin: another way to Control or survey Antimicrobial Resistance

2019 ◽  
Author(s):  
VICTORIEN DOUGNON ◽  
Phénix ASSOGBA ◽  
Jean-Pierre GNIMATIN ◽  
Jerrold AGBANKPE ◽  
Hornel KOUDOKPON ◽  
...  
Keyword(s):  
Public Health ◽  
Mathematical Modeling ◽  
Escherichia Coli ◽  
Developing Countries ◽  
Urinary Tract ◽  
Antimicrobial Resistance ◽  
Klebsiella Pneumoniae ◽  
Urinary Tract Infections ◽  
Urine Samples ◽  
Tract Infections

Abstract Background: Infectious diseases are serious public health issue both in developing countries and industrialized countries. In developing countries, they are the main cause of high mortality rates. In the second group, existing resistance to antibiotics is developing growing at an alarming rate. The purpose of this study was to produce data of national interest to implement sustainable control of antimicrobial resistance as well as it spreads. Methods: One hundred ninety (190) urine samples were collected in several hospitals in Benin from patients suspected of having a urinary tract infection. After getting the inform consent from patients, samples collections were performed under aseptic conditions and were further subjected to bacteriological tests in the laboratory. The resistance profile of the bacterial strains identified was then established. The search for betalactamase production was performed by the synergy test between amoxicillin + clavulanic acid and cephalosporins. Mathematical modeling of the resistance of the strains identified by 2024 was finally carried out using compartmental deterministic models. Results: Two hundred thirty (230) strains were identified from urine samples. Male individuals were the most affected by urinary tract infections. Individuals in the 21-30 age groups were predominant. Escherichia coli was the most isolated bacterial species (32.43%) in this study followed by Klebsiella pneumoniae (26.85%) and Enterobacter cloaceae (25.92%). The susceptibility testing of isolates bacteria to antibiotics showed a strong resistance of strains to amoxicillin (91.82%). The lowest resistance obtained was observed with imipenem (2%). The betalactamase was produced by 24.03% of the strains identified. Escherichia coli (32.43%) was indeed the most productive of betalactamase followed by Klebsiella pneumoniae (31.03%). Mathematical modeling revealed a rampant rise in the resistance of bacteria to the antibiotics tested. Conclusions: These results provide important data for public health. They deserve constructive advocacy so that more specific actions are taken in relation to antimicrobial resistance.

scholarly journals MP23-13 ANTIMICROBIAL RESISTANCE AND EXTENDED SPECTRUM BETA-LACTAMASE AGENTS IN URINARY TRACT INFECTIONS: A SERIOUS PROBLEM IN THE NORTH OF MEXICO

2018 ◽  
Vol 199 (4S) ◽  
Author(s):  
José Iván Robles-Torres ◽  
Lauro Gómez-Guerra ◽  
Elvira Garza-González ◽  
Soraya Mendoza-Olazarán ◽  
Samantha Flores-Treviño ◽  
...  
Keyword(s):  
Urinary Tract ◽  
Antimicrobial Resistance ◽  
Urinary Tract Infections ◽  
Beta Lactamase ◽  
Extended Spectrum Beta Lactamase ◽  
The North ◽  
Extended Spectrum ◽  
Tract Infections

scholarly journals Prevalence of CTX-M beta-Lactamases in Escherichia coli from community-acquired urinary tract infections and associated risk factors among women in Cameroon

2017 ◽  
Vol 3 (1) ◽  
pp. 51 ◽  
Author(s):  
Ingrid Cecile Djuikoue ◽  
Omer Njajou ◽  
Hortense Gonsu Kamga ◽  
Charles Fokunang ◽  
Adamo Bongoe ◽  
...  
Keyword(s):  
Risk Factors ◽  
Escherichia Coli ◽  
Developing Countries ◽  
Urinary Tract ◽  
Urinary Tract Infections ◽  
Intestinal Flora ◽  
Beta Lactamase ◽  
Sample Collection ◽  
Associated Risk Factors ◽  
Tract Infections

Background: In recent years, a worldwide dissemination of CTX-M beta-lactamase-type in Escherichia coli strains isolates from community-acquired urinary tract infections (CA-UTI) has been observed. However little is known on the prevalence and risk factors of this global threat in developing countries. Objective: The aim of this study was to study the prevalence and risk factors for CA-UTI in Yaoundé, Cameroon. Methods: Eighty six patients with urinary E.coli infection recruited from 10 health structures in the town of Yaoundé, Cameroon. After taking the first urine, faeces were collected from the patients for the study of the intestinal flora. The sample collection of faeces was done on a selected gel of enterobacteria resistant to third generation of cephalosporin. The molecular typing of extended-spectrum -lactamase (ESBL) was carried out. Results: Eighty-six strains of E. coli from 86 patients were included. We found that 39 (45.3%) strains produced an extendedspectrum beta-lactamase. Among risk factors, previous use of antibiotic and the dry season were associated with the presence of an ESBL-producing strain in the urine. All ESBL were identified as CTX-M. The production of CTX-M was found to be significantly associated with resistance to fluoroquinolones, aminoglycosides and to the association of trimethoprim-sulfamethazole. Conclusions: The prevalence of CTX-M ESBL in Yaoundé, Cameroon, provides new evidence on the global dissemination of CTX-M and the extent of this phenomenon in developing countries.

Profiles and genetic determinants of antimicrobial resistance in pathogens causing community-acquired urinary tract infections in reproductive-age women

2021 ◽  
pp. 12-15
Author(s):  
T. A. Khusnutdinova ◽  
E. V. Shipitsyna ◽  
A. A. Krysanova ◽  
A. M. Savicheva
Keyword(s):  
Urinary Tract ◽  
Antimicrobial Resistance ◽  
Urinary Tract Infections ◽  
Reproductive Age ◽  
Beta Lactamase ◽  
Beta Lactam ◽  
Genetic Determinants ◽  
Women Of Reproductive Age ◽  
Beta Lactamases ◽  
Tract Infections

Objective. To characterize the profiles and genetic determinants of antibiotic resistance in pathogens of community-acquired urinary tract infections (UTI) in women of reproductive age in St. Petersburg (Russia).Materials and methods. The study included strains of microorganisms obtained from 145 women of reproductive age with diagnosed with a UTI. Antibiotic susceptibility was evaluated by disk diffusion method; the interpretation of the results was carried out in accordance with the EUCAST criteria. Strains of uropathogenic enterobacteria were tested by PCR for the presence of beta-lactamase genes: beta-lactamase genes encoding enzymes of the AmpC group (MOX, CMY, LAT, BIL, DHA, ACC, MIR, ACT, FOX), TEM, SHV, OXA‑1, ESBL genes of the CTX–M group, genes of carbapenemases (KPC, OXA‑48) and metal-beta-lactamases (VIM, IMP, NDM).Results. Most cases (81 %) of UTIs in women in St. Petersburg were due to enterobacteria, with Escherichia coli highly prevailing (66 %). Fosfomycin, meropenem and nitrofurantoin had the highest in vitro activity against uropathogenic Enterobacteriaceae. High rates of resistance to betalactam antibiotics were found (from 16 % to cefotaxime to 28 % to amoxicillin/clavulanic acid). The genes of TEM-beta-lactamases were found in 31 isolates (26.7 %), SHV – in 17 (14.6 %), CTX–M type – in 15 (12.9 %), DHA – in 2 (1.7 %). The other beta-lactam resistance genes (MOX, CMY, LAT, BIL, ACC, MIR, ACT, FOX, KPC group, OXA-like group, VIM, IMP, NDM) were not detected.Conclusion. Microbiological and molecular analysis of the structure of beta-lactam resistance is important for the effective of epidemiological control of antimicrobial resistance in pathogens causing urinary tract infections.

Frequency and Antibiotic Resistance Profile of Uropathogens Associated With Community Acquired Urinary Tract Infections

QJM ◽  
2021 ◽  
Vol 114 (Supplement_1) ◽  
Author(s):  
Sally Mohammed Saber ◽  
Marwa Abd el Rasoul El-Ashry ◽  
Christina Magdy Lotfy Mikhail Assad
Keyword(s):  
Antibiotic Resistance ◽  
Urinary Tract ◽  
Antimicrobial Resistance ◽  
Urinary Tract Infections ◽  
Bacterial Growth ◽  
Empirical Treatment ◽  
Urine Samples ◽  
University Hospitals ◽  
Geographical Variations ◽  
Tract Infections

Abstract Background Community acquired urinary tract infections (CAUTIs) are among the most common bacterial infections diagnosed in community health practice resulting in high rates of morbidity and high economic costs associated with its treatment. CAUTIs are usually treated empirically. Geographical variations in etiologic agents and their antibiotic sensitivity patterns are common. Knowledge of antibiotic resistance trends is important for improving evidence-based recommendations for empirical treatment of UTIs. Adequate empirical treatment can cause decrease in unnecessary hospital stays, treatment cost, antibiotic side effects as well as reducing antimicrobial resistance spread. Aim of the Work To determine the implicated uropathogens associated with community-acquired UTIs and their respective antimicrobial resistance pattern to improve the efficacy of empirical treatment of this infection. Patients and Methods This a cross-sectional descriptive study conducted from November 2018 to November 2019 at the main Microbiology Lab in Clinical Pathology Department, Ain Shams University Hospitals. Samples were received from patients attending different outpatient clinics in Ain Shams University Hospitals having UTI symptoms and whose urine samples showed significant bacterial growth (≥105 CFU/mL) associated with pyuria (more than 8–10 pus cells by microscopic urine analysis) and nitrite and leukocyte esterase positive by chemical analysis of urine strip. Results A total of 1600 patients who fulfilled our inclusion criteria were sampled. Of these, 1058 patients had urine samples that showed significant bacterial growth while about 34% of the samples were contaminated and excluded . Of the 1058 significant samples, 764 (72.21%) were from females and 294 (27.79%) from males with female to male ratio 2.6:1. Escherichia coli was found the dominant bacteria among a with the prevalence rate of 24.4% followed by Klebsiella pneumoniae (13.8%). Tetracycline was found the most resistant antibiotic 929 (95.4%) followed by Ampicillin, Tazobactam-piperacillin, and Ampicillin- sulbactam by 90.1%, 81.7% and 77.7% respectively; however, the most sensitive antibiotics against all uropathogens were Carbapenems (Ertapenem 72.9% fol Imipenem 66.5% and Meropenem 66.4%) followed by Nitrofurantoin and Amikacin showing 64.3% and 58.01% sensitivity respectively . However, For gram positive uropathogens, Linezolid showed the highest sensitivity in 84% of the isolates. Conclusion The present study reveals a familiar pattern with respect to the species of uropathogens involved in community acquired urinary tract infections, however with considerable bacterial resistance to common empirically prescribed antibiotics.

Sign in / Sign up

Export Citation Format

Share Document