scholarly journals Microbial Remediation of Used Engine Oil from Contaminated Soil around Automobile Workshop in Calabar Metropolis, Cross River State, Nigeria

2019 ◽  
pp. 1-12 ◽  
Author(s):  
A. B. Andem ◽  
I. U. Bassey ◽  
C. O. Odey ◽  
O. R. Ibor ◽  
I. O. Agborubere
Keyword(s):  
Organic Carbon ◽  
Total Organic Carbon ◽  
Contaminated Soil ◽  
Control Site ◽  
Engine Oil ◽  
Contaminated Site ◽  
Indigenous Bacteria ◽  
Total Hydrocarbon ◽  
Staphylococcus Spp ◽  
Clostridium Spp

Microbial biodegradation of engine oil contaminated soil in Calabar Metropolis was studied for a period of six (6) months (January to June, 2017). The soil samples collected were ice boxed and taken to the laboratory for microbial, total hydrocarbon, total organic carbon and other physico-chemical parameters analysis. A total of thirteen (13)  indigenous bacteria species were identified in the soil of the sites analyzed during the study, which includes; Staphylococcus spp., Pseudomonas aeruginosa, Bacillus spp., E. coli, Enterococcus feacalis, Shigella spp., Arthrobacter spp., Alcaligen spp., Acinobacter spp., Azotobacter spp., Aeromonas spp., Xanthomonas spp. and Clostridium spp. The most abundant bacteria in the contaminated site was Staphylococcus spp. (65%) while the least bacteria count in the contaminated site was Clostridium spp. (9%). Staphylococcus spp. was the most abundant indigenous bacterial species and also the most effective biodegradation bacteria. The identified indigenous bacteria utilized the hydrocarbons, multiplied rapidly and then degraded the total hydrocarbon and total organic carbon more in the contaminated site compared to the control site. Site one recorded the highest bacteria count (927) while the least bacteria counts were recorded in the control site (81). The bacteria species showed its degradation and bioremediation capabilities prompting the need for its use in cleaning crude oil contaminated sites, due to the fact that it is cheap and not environmentally harmful.

scholarly journals Phenylobacterium Korensee Best Indigenous Petroleum Hydrocarbon Degrading Bacteria Isolated from Contaminated Soil of Bahror, Alwar Region, India

2019 ◽  
Vol 10 (08) ◽  
pp. 20203-20211 ◽  
Author(s):  
Mohan Lal Kuri ◽  
Vidhya Kumari ◽  
Shikha Roy
Keyword(s):  
Contaminated Soil ◽  
Engine Oil ◽  
Contaminated Site ◽  
Spectroscopy Analysis ◽  
Global Issue ◽  
Degrading Bacteria ◽  
Term Basis ◽  
Agar Media ◽  
Gas Chromatography Mass Spectroscopy

Contamination of soil, water and air due to hydrocarbons are a global issue and bioremediation provides probably the best way to remediate the contaminants. The current study shows the biodegradation of crude oil, diesel and used engine oil by a newly isolated Phenylobacterium korensee from contaminated soil of Bahror, Alwar, Rajasthan. Hydrocarbon degrading strain was screened on BHA (Bushnell Haas Agar) media supplemented with 2T engine oil as sole carbon source. The strain was found to be degrading at 1%, 4% and 10% of used 2T engine oil respectively after 14 days. Degradation was confirmed both gravimetrically and by Gas Chromatography Mass Spectroscopy analysis. The degradation was found very well at long term basis. The optimization of growth also studied at temperature and pH basis also. The significance of the study is that the percentage degradation of the complex petroleum supplements used in the study was found to be far higher than some of the previously reported values and this bacterial strain was firstly found from this contaminated site.

scholarly journals Microbial Treatment of Soil Contaminated with Spent engine Oil / Biotreatment of Soil Contaminated with Spent Engine by Microorganisms

2018 ◽  
Author(s):  
A. A. Ayandele
Keyword(s):  
Organic Matter ◽  
Contaminated Soil ◽  
Heterotrophic Bacteria ◽  
Ph Value ◽  
Soil Samples ◽  
Engine Oil ◽  
Contaminated Site ◽  
Degrading Bacteria ◽  
Spent Engine Oil ◽  
Test Organisms

AbstractThe potential of six microorganisms (Pseudomonas aeruginosa, Micrococcus sp, Flavobacterium sp, Rhizopus sp, Penicillium sp and Fusarium sp) isolated from hydrocarbon contaminated site were evaluated for their biodegradation ability. The soil samples were contaminated with 5% (w/v) of spent engine oil and the rate of biodegradation of the oil was studied for a period of 10weeks under greenhouse experiment. The total heterotrophic bacteria count (THBC), total hydrocarbon degrading bacteria count (THDBC), physicochemical and heavy metals properties of the soil samples and Total Petroleum Hydrocarbon (TPH) were determined after treatment with test organisms. THBC and THDBC ranged from 0.175 to 0.280 CFUg-1 and 0.47 CFUg-1 respectively for the control plot, while THBC is ranging from 0.197 to 0.275 CFUg-1 and THDBC was 0.180 to 0. 473 CFUg-1 for the contaminated plot. There was a slight increase in the pH value of the contaminated soil sample and the treated soil samples as the experimental weeks increased. The results obtained showed a significant decrease (at p ≤ 0.05) in the nutrients content of the soil samples. There was an increase from 1.09 in the control to 15.5% in the content of organic matter after contamination and from 1.88% to 26.8% in the % of organic matter too. There was a significant reduction (at p ≤ 0.05) in the concentration of Fe, Zn, Pb, Cd, Cu, Cr and Ni after 10 weeks of incubation with the tested organisms. Plant growth in the treated contaminated soil samples ranged from 32.6cm to 38.6cm, while that of the control 1 (Uncontaminated soil) was 51.2cm and 19.7cm high was observed in the Control 2 (contaminated untreated soil) after 22 days of the experiment. The TPH degradation (% loss) ranged from 79.7 to 89. 2% after 10 weeks of treatment. P. aeruginosa had the highest level of degradation (89.2%), while Micrococcus sp and Rhizopus sp had the least degradation at 79.9%.All the microorganisms used in this study had the abilities to remediating soil contaminated with spent engine oil and the remediated soil samples were able to support the growth of Zea mays at 5% (w/v) level of contamination.

scholarly journals Phytoremediation of Copper and Nickel Contaminated Soil Using Eleusine indica on Soil Properties

2020 ◽  
pp. 1-8
Author(s):  
G. I. Ameh ◽  
E. J. Onuh
Keyword(s):  
Organic Carbon ◽  
Moisture Content ◽  
Soil Properties ◽  
Total Organic Carbon ◽  
Cation Exchange ◽  
Contaminated Soil ◽  
Cation Exchange Capacity ◽  
Exchange Capacity ◽  
Soil Samples ◽  
Eleusine Indica

The effects of Eleusine indica phytoremediation on the soil properties of Copper and Nickel contaminated soil samples were investigated using standard techniques. Soil sample and seeds of E. inidca were collected from a farmland in Obe, Nkanu West L.G.A of Enugu State. 0%, 1%, 2% and 3% potted treatments of Copper and Nickel contaminated soil were made for three samples (initial sample, sample without plant and sample with plant). The plant showed BAF and TF greater than 1. The Soil samples after the plant was harvested were subjected to soil analysis tests (Metal determination, pH, Soil moisture content, total organic carbon, and cation exchange capacity). The results from the study revealed that soil samples with plant showed a lower pH level within the range of 6.50 to 7.80 and higher percentage moisture content for both Cu and Ni contaminated soil samples, of which 1% treatment showed the highest percentage moisture of 0.679% for Cu contamination and 3.16% for Ni contamination. Heavy metal contamination of soil reduces its total organic carbon and increases its cation exchange capacity. Eleusine indica is a hyperaccumulator, its root stores more heavy metals and thus suitable for phytoremediation.

scholarly journals Biodegradation of used Engine Oil Contaminated Site using Individual and Mixed bacterial isolates

2020 ◽  
pp. 38-45
Author(s):  
Muh'd A. J. ◽  
Sa'adatu A.Y. ◽  
Surayya M. M. ◽  
Sa'adatu M. I. ◽  
Nafisa B. ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Bacillus Cereus ◽  
Contaminated Soil ◽  
Bacterial Species ◽  
Soil Samples ◽  
Engine Oil ◽  
Contaminated Site ◽  
Bacterial Isolates ◽  
The World ◽  
Degradation Ability

Oil is most widely distributed source of energy in the world and large-scaled environmental pollutant. Oil, oil products, and oil containing industrial waste pollution is ranked second place after radioactive pollution on account of their harmful action to ecosystems. Contamination of soil by organic chemicals (mostly hydrocarbons) is prevalent in oil producing and industrialized countries of the world. Biodegradation, a strategy that uses biological means (i.e microbes) to degrade, stabilize and remove soil contaminants is an alternative green technology remediation of hydrocarbon contaminated soil. This study was carried out to isolate and screen Bacteria capable of degrading used engine oil from oil contaminated site. Soil samples were collected from oil contaminated site. Bacterial species were isolated from the collected soil samples by serial dilution and agar methods. Different bacterial species were isolated but only four were oil degrading isolates. The identity of the various genera of bacterial contaminants were determined by a combination of cultural, morphological as well as preliminary biochemical characteristics of the isolates. The four oil degrading bacteria (AJ1, AJ2, AJ3 and AJ4) were preliminarily identified as Bacillus cereus, Pseudomonas aeruginosa, Bacillus subtillis and Micrococcus spp respectively. The degradation ability of the bacterial isolates was screened and maximum degradation was recorded by AJ 5 (Mixed culture) with 66.9 %, followed by Bacillus cereus (50.3 %), Bacillus subtilis (44 %), Pseudomonas aeruginosa (37.9 %) and the least was seen in Micrococcus spp (35.3 %). These findings revealed that some bacteria species are capable of utilizing the oil and used it as sole source of carbon and energy and the mixed consortia of the bacteria have rapid degradation ability. Biological degradation of hydrocarbon contaminated soil offers a better and more environmentally friendly technique that if properly explored can bring our environment into a better place for both plant and animal.

CHARACTERISATION OF DISSOLVED ORGANIC CARBON (DOC) LEACHED FROM LEAVES IN WATER

2008 ◽  
Vol 6 (1) ◽  
Author(s):  
Markus Heryanto Langsa
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Organic Carbon ◽  
Dissolved Organic Carbon ◽  
Total Organic Carbon ◽  
Gas Chromatography Mass Spectrometry ◽  
Pyrolysis Gas ◽  
Pyrolysis Gas Chromatography ◽  
Chromatography Mass Spectrometry ◽  
Pinus Radiate

<p>Penelitian ini bertujuan untuk menentukan senyawa organik khususnya organic karbon terlarut (DOC) dari dua spesies daun tumbuhan (<em>wandoo eucalyptus </em>and <em>pinus radiate, conifer</em>) yang larut dalam air selama periode 5 bulan leaching eksperimen. Kecepatan melarutnya senyawa organic ditentukan secara kuantitatif dan kualitatif menggunakan kombinasi dari beberapa teknik diantaranya Total Organic Carbon (TOC) analyser, Ultraviolet-Visible (UV-VIS) spektrokopi dan pyrolysis-gas chromatography-mass spectrometry (Py-GC-MS).</p><p>Hasil analisis DOC dan UV menunjukkan peningkatan yang tajam dari kelarutan senyawa organic di awal periode pengamatan yang selanjutnya berkurang seiring dengan waktu secara eksponensial. Jumlah relatif senyawa organic yang terlarut tergantung pada luas permukaan, aktifitas mikrobiologi dan jenis sampel tumbuhan (segar atau kering) yang digunakan. Fluktuasi profil DOC dan UV<sub>254</sub> disebabkan oleh aktifitas mikrobiologi. Diperoleh bahwa daun kering lebih mudah terdegradasi menghasilkan senyawa organic dalam air dibandingkan dengan daun segar. Hasil pyrolysis secara umum menunjukkan bahwa senyawa hidrokarbon aromatic dan fenol (dan turunannya) lebih banyak ditemukan pada residue sampel setelah proses leaching kemungkinan karena adanya senyawa lignin atau aktifitas humifikasi mikrobiologi membuktikan bahwa senyawa-senyawa tersebut merupakan komponen penting dalam proses karakterisasi DOC.</p>

Total organic carbon and the preservation of organic-walled microfossils in Precambrian shale

Geology ◽  
2020 ◽  
Author(s):  
C.R. Woltz ◽  
S.M. Porter ◽  
H. Agić ◽  
C.M. Dehler ◽  
C.K. Junium ◽  
...  
Keyword(s):  
Species Richness ◽  
Organic Carbon ◽  
Total Organic Carbon ◽  
Microbial Degradation ◽  
Sedimentation Rate ◽  
Surface Pattern ◽  
Causal Factors ◽  
Eukaryotic Diversity ◽  
High Concentrations ◽  
Fossil Preservation

Much of our understanding of early eukaryote diversity and paleoecology comes from the record of organic-walled microfossils in shale, yet the conditions controlling their preservation are not well understood. It has been suggested that high concentrations of total organic carbon (TOC) inhibit the preservation of organic fossils in shale, and although this idea is supported anecdotally, it has never been tested. Here we compared the presence, preservational quality, and assemblage diversity of organic-walled microfossils to TOC concentrations of 346 shale samples that span the late Paleoproterozoic to middle Neoproterozoic in age. We found that fossil-bearing samples have significantly lower median TOC values (0.32 wt%, n = 189) than those containing no fossils (0.72 wt%, n = 157). Preservational quality, measured by the loss of surface pattern, density of pitting, and deterioration of wall margin, decreases as TOC increases. Species richness negatively correlates with TOC within the ca. 750 Ma Chuar Group (Arizona, USA), but no relationship is observed in other units. These results support the hypothesis that high TOC content either decreases the preservational quality or inhibits the preservation of organic-walled microfossils altogether. However, it is also possible that other causal factors, including sedimentation rate and microbial degradation, account for the correlation between fossil preservation and TOC. We expect that as TOC varies in space and time, so too does the probability of finding well-preserved fossils. A compilation of 13,940 TOC values spanning Earth history suggests significantly higher median TOC levels in Mesoproterozoic versus Neoproterozoic shale, potentially biasing the interpreted pattern of increased eukaryotic diversity in the Tonian.

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