scholarly journals Antibacterial and Hemagglutinating Activity of the Fruit Pulp of Trilepisium madagascariense DC

2017 ◽  
Vol 18 (2) ◽  
pp. 1-9
Author(s):  
Oludele Odekanyin ◽  
Temitope Aibinu ◽  
Olutayo Abioye
2013 ◽  
Vol 20 (5) ◽  
pp. 499-509 ◽  
Author(s):  
Pramod Kumar ◽  
Dipak N. Patil ◽  
Anshul Chaudhary ◽  
Shailly Tomar ◽  
Dinesh Yernool ◽  
...  

2020 ◽  
Vol 27 ◽  
Author(s):  
Edgar Acedo-Espinoza ◽  
Irlanda Lagarda-Diaz ◽  
Rosina Cabrera ◽  
Ana M. Guzman-Partida ◽  
Amir Maldonado-Arce ◽  
...  

Background: The O. tesota lectin PF2 is a tetrameric protein with subunits of 33 kDa that recognizes only complex carbohydrates, resistant to proteolytic enzymes and has insecticidal activity against Phaseolus beans pest. Objective: To explore PF2 lectin features at different protein structural levels and to evaluate the effect of temperature and pH on its functionality and conformational stability. Methods: PF2 lectin was purified by affinity chromatography. Its primary structure was resolved by mass spectrometry and analyzed by bioinformatic tools, including its tertiary structure homology modeling. The effect of temperature and pH on its conformational traits and stability was addressed by dynamic light scattering, circular dichroism, and intrinsic fluorescence. The hemagglutinating activity was evaluated using a suspension of peripheral blood erythrocytes. Results: The proposed PF2 folding comprises a high content of beta sheets. At pH 7 and 25 °C, the hydrodynamic diameter (Dh) was found to be 12.3 nm which corresponds to the oligomeric native state of PF2 lectin. Dh increased under the other evaluated pH and temperature conditions, suggesting protein aggregation. At basic pH, PF2 exhibited low conformational stability. The native PF2 (pH 7) retained its full hemagglutinating activity up to 45 °C and exhibited one transition state with a melting temperature of 76.8 °C. Conclusion: PF2 showed distinctive characteristics found in legume lectins. The pH influences the functionality and conformational stability of the protein. PF2 lectin displayed a relatively narrow thermostability to the loss of secondary structure and hemagglutinating activity.


Author(s):  
Mona A. Mohammed ◽  
Hanan Naeim Attia ◽  
Souad E. El-Gengaihi ◽  
Yousreya A. Maklad ◽  
Kawkab A. Ahmed ◽  
...  

2021 ◽  
Vol 270 ◽  
pp. 113813
Author(s):  
Djabir Daddiouaissa ◽  
Azura Amid ◽  
Muhamad Shirwan Abdullah Sani ◽  
Ahmed A.M. Elnour

Horticulturae ◽  
2021 ◽  
Vol 7 (7) ◽  
pp. 165
Author(s):  
Allan Waniale ◽  
Rony Swennen ◽  
Settumba B. Mukasa ◽  
Arthur K. Tugume ◽  
Jerome Kubiriba ◽  
...  

Seed set in banana is influenced by weather, yet the key weather attributes and the critical period of influence are unknown. We therefore investigated the influence of weather during floral development for a better perspective of seed set increase. Three East African highland cooking bananas (EAHBs) were pollinated with pollen fertile wild banana ‘Calcutta 4′. At full maturity, bunches were harvested, ripened, and seeds extracted from fruit pulp. Pearson’s correlation analysis was then conducted between seed set per 100 fruits per bunch and weather attributes at 15-day intervals from 105 days before pollination (DBP) to 120 days after pollination (DAP). Seed set was positively correlated with average temperature (P < 0.05–P < 0.001, r = 0.196–0.487) and negatively correlated with relative humidity (RH) (P < 0.05–P < 0.001, r = −0.158–−0.438) between 75 DBP and the time of pollination. After pollination, average temperature was negatively correlated with seed set in ‘Mshale’ and ‘Nshonowa’ from 45 to 120 DAP (P < 0.05–P < 0.001, r = −0.213–−0.340). Correlation coefficients were highest at 15 DBP for ‘Mshale’ and ‘Nshonowa’, whereas for ‘Enzirabahima’, the highest were at the time of pollination. Maximum temperature as revealed by principal component analysis at the time of pollination should be the main focus for seed set increase.


2013 ◽  
Vol 60 (5) ◽  
pp. 526-530 ◽  
Author(s):  
Yun-Wen Liao ◽  
Chiy-Rong Chen ◽  
Jiunn-Jye Chuu ◽  
Hui-Chi Huang ◽  
Jue-Liang Hsu ◽  
...  

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