scholarly journals Callus Culture as an Alternative Source of Secondary Metabolites in Curtailing Malaria Epidemic

2021 ◽  
pp. 23-31
Author(s):  
Daniel Thakuma Tizhe ◽  
Gali Adamu Ishaku ◽  
Afiniki Yohanna ◽  
Dashe Dentsen Fortune ◽  
Aisha Salihu Jibrin
Keyword(s):  
In Vitro Culture ◽  
Plant Species ◽  
Callus Culture ◽  
Public Healthcare ◽  
Natural Habitats ◽  
Alternative Source ◽  
Natural Form ◽  
Continuous Increase ◽  
Wide Range

Malaria remains a threat to public healthcare system. In 2018, more than 200 million people were exposed to this disease globally. There have been reports of drug-resistance in the recommended therapy in some endemic regions. This called for relentless efforts in the search for potential antimalarial compounds. An in vitro culture technique has emerged as a promising option for sustainable and industrial propagation of plant bioactive compounds with wide range of medicinal properties. The demand for these invaluable metabolites is witnessing a continuous increase as a folk medicine, hence, endangering their existence in natural habitats. Besides its use in natural form, the nature’s gift to humans seems to be restricted and limited by environmental conditions. An in vitro culture approach remains the most viable and sustainable alternative for the endangered plant species. Here, we present some plant species reported to have potential antimalarial activities and recommend further study through callus culture induction against malaria.

scholarly journals Inhibition of browning problem during the callogenesis of Spartium junceum L.

2021 ◽  
Vol 27 (1) ◽  
pp. 68-77
Author(s):  
Mina Taghizadeh ◽  
Mahboubeh Ganji Dastjerdi
Keyword(s):  
In Vitro Culture ◽  
Callus Formation ◽  
Nodal Explants ◽  
Antioxidant Compounds ◽  
Induction Phase ◽  
Natural Habitats ◽  
Running Water ◽  
Changing Light ◽  
Spartium Junceum

Abstract During different phases of in vitro culture, plant tissues may be exposed to some stresses that never encounter in their natural habitats. The most significant stresses which interfere with in vitro culture are pathogenic contamination and browning disorder. Since browning sign is occurred during all phases of in vitro culture of Spartium junceum L., the present study was done preventing explants from browning during disinfection and callogenesis phases using exposure time of sterilants (ethanol 0, 30, 60 s and home bleach 0, 10, 15 min), antioxidant compounds (PVP 0.5%, Activated charcoal 0.1%, Curcumin 0.1%), Running water (30 and 60 min) plant growth regulators (2,4-D 0, 0.5, 1 and 2 mg L-1 and BA 0, 0.1 and 0.2 mg L-1), and by changing light/dark conditions was designed. The results showed that ethanol 70% (30 s) in combination with home bleach 20% (10 min) had the best effect in control contaminations and browning sign in nodal explants of S. junceum. The application of PVP 0.5% in medium was the best treatment to control of browning nodal explants in callus induction phase. The highest callus formation and the lowest explant browning were obtained on the medium supplemented with 0.5 mg L-1 2,4-D under the darkness condition. According to the results of this study, how disinfection methods, culture medium compositions and light conditions were effective on the browning and callogenesis of Spartium junceum L.

scholarly journals Regeneration of Cucumis melo L. plants from cell suspension derived from hypocotyls callus

2013 ◽  
Vol 7 (3) ◽  
pp. 23-28
Author(s):  
Jamella Hazza Rasheed
Keyword(s):  
In Vitro Culture ◽  
Cell Suspension ◽  
Callus Culture ◽  
Cucumis Melo ◽  
Ms Medium ◽  
Regeneration Capability ◽  
Cucumis Melo L

This study aimed to investigate the regeneration capability of cell suspension derived from hypocotyl callus of the vegetable plant, muskmelon, Cucumis melo L. Culture of the densities (7.5, 9.8, 13.0, 11.2, 7.8, 4.2 )× 102 cell/ml produced callus primordia which formed typical callus culture successfully. This green – yellowish color and semi-compact callus regenerated shoots on agar solidified MS medium supplemented with 2.0mgL-1BA. These regenerates rooted readily in MS0 medium. They were adapted and routinely transferred to soil. The conclusion of this work that muskmelon plants have a good response to in vitro culture with no need to specific requirements.

scholarly journals Antibacterial and Antifungal Activities of Ethiopian Medicinal Plants: A Systematic Review

2021 ◽  
Vol 12 ◽  
Author(s):  
Dereje Nigussie ◽  
Gail Davey ◽  
Takele Beyene Tufa ◽  
Malcolm Brewster ◽  
Belete Adefris Legesse ◽  
...  
Keyword(s):  
Medicinal Plants ◽  
Plant Species ◽  
Meta Analysis ◽  
Fungal Species ◽  
Antifungal Activities ◽  
Traditional Medicines ◽  
Antibacterial And Antifungal Activities ◽  
Wide Range ◽  
Antibacterial And Antifungal

Background: Podoconiosis and lymphatic filariasis are the most common causes of lower limb lymphoedema in the tropics. Many sufferers experience frequent painful episodes of acute bacterial infection. Plant based traditional medicines are used to treat infections in many countries and are culturally established in Ethiopia. Ethiopian medicinal plants found to have antibacterial and antifungal activities were reviewed with the aim of increasing information about the treatment of wound infections in patients with lymphoedema.Methods: This study collates data from published articles on medicinal plants with antibacterial and antifungal activities in Ethiopia. A systematic search of Scopus, EMBASE, PUBMED/MEDLINE and Google Scholar was undertaken. The Preferred Reporting Items for Systematic Reviews and Meta-analysis (PRISMA) guidelines were followed. The protocol was registered on PROSPERO with registration number CRD42019127471. All controlled studies of in vitro antibacterial and antifungal activities were considered. All articles containing the descriptors published until June 28, 2019 were included. The outcome was measured as percent inhibition of microbial growth. For quality assessment of individual in vitro studies, OECD guidelines and the WHO-Good Laboratory Practice (GLP) handbook were used.Results: Seventy-nine studies met the inclusion criteria. A total of 150 plant species and three compounds had been tested against 42 species of bacteria, while 43 plant species had been tested against 22 species of fungus.Conclusion: Materials derived from several Ethiopian medicinal plants have been shown to have promising activity against a variety of bacteria and fungi. Those derived from Azadiractha indica A. Juss. and Lawsonia inerms L. are the most extensively studied against a wide range of gram-negative and positive bacteria, and fungal species.

scholarly journals Pre-forcing Treatments Influence Bud Break and Shoot Elongation in Forced Woody Species

1992 ◽  
Vol 10 (2) ◽  
pp. 101-103
Author(s):  
Guochen Yang ◽  
Paul E. Read
Keyword(s):  
In Vitro Culture ◽  
Plant Species ◽  
Woody Plants ◽  
Woody Plant ◽  
Woody Species ◽  
Extended Period ◽  
Shoot Elongation ◽  
Bud Break ◽  
Cold Requirement

Abstract Experiments were conducted to evaluate the feasibility of pre-forcing treatments for the release of bud dormancy of dormant stems of lilac, privet and Vanhoutte spirea. The new softwood growth of these dormant stems was used either as explants for in vitro culture or as cuttings for rooting studies of woody plant species in the off-season. A pre-forcing 15% bleach solution (0.78% NaOCl) soak hastened bud break, enhanced percentage of bud break, and promoted shoot elongation. Pre-forcing wetting agent treatments produced similar results to those of the bleach soak with variation among wetting agents and plant species. Smaller treatment differences were observed in the forcing characteristics when stems were collected later in the winter, probably because the cold requirement of the buds had been completely or partially met. This technique will provide explants for in vitro culture and softwood cuttings for propagation of woody plants over an extended period.

Enhanced production of bioactive compound in the callus culture of Glycyrrhiza glabra L. with the improved biomass accumulation

2021 ◽  
Author(s):  
Nancy Jaiswal ◽  
Yashodhara Verma ◽  
Pragati Misra
Keyword(s):  
Callus Culture ◽  
Biomass Accumulation ◽  
Bioactive Compound ◽  
Callus Cultures ◽  
Glycyrrhiza Glabra ◽  
Enhanced Production ◽  
Adenine Sulphate ◽  
Wide Range ◽  
Metabolite Content

Abstract Licorice ( Glycyrrhiza glabra ) of Fabaceae family is known to have wide range of medicinal properties due to the metabolites found in the plant’s tissue. Callus cultures from Glycyrrhiza glabra were previously initiated in vitro using leaf as an explant. The current study was designed in a way to examine the possible role of different elicitors concentrations on the stimulation of biomass and their effects on different metabolite content such as total carbohydrates, protein, proline, phenol, alkaloid, flavonoid and glycyrrhizin. Elicitation with different concentration of elicitors increased the biomass and metabolite content in callus culture of G. glabra at different rates. The optimum concentration of adenine sulphate for maximum biomass accumulation (16.79 g/flask) was found to be at 50 mg/l on the incubation of 20 th days. Adenine sulphate as well as putrescine was also found to stimulate the different metabolite content to 3-4 folds in the callus cultures as compared to that of control. Results showed that the metabolite content and antioxidant enzyme of Glycyrrhiza glabra can be enhanced by appropriate forms of elicitation.

Savanna woody plants and their provision of food resources to bees in southern Burkina Faso, West Africa

2020 ◽  
Vol 5 (1) ◽  
pp. 14-23 ◽  
Author(s):  
Coulibaly Drissa ◽  
Yalamoussa Tuo ◽  
Mouhamadou Koné ◽  
Larba Hubert Balima ◽  
Souleymane Konaté ◽  
...  
Keyword(s):  
Land Use ◽  
West Africa ◽  
Plant Species ◽  
Woody Plant ◽  
Food Resources ◽  
Native Plant ◽  
Natural Habitats ◽  
Woody Plant Species ◽  
Wide Range ◽  
Pollen Species

West African savanna ecosystems and biodiversity are severely threatened by intensified land use and increasing degradation of natural habitats. Despite the importance of bees for pollinating crops and native plant species little information is available regarding the importance of savanna woody plant species to provide bees with food resources. Flora inventories were carried out on 48 subplots laid out across three land use types. The number of bee morphospecies and their abundance as flower visitors were recorded from inflorescences of plants during the different flowering periods. Out of a total diversity of 82 woody plant species, 53 species (64.63%) from 38 genera and 21 families were melliferous. These plants were visited by bees for foraging nectar and/or pollen. Species of the Combretaceae family were the most visited by bees in terms of individuals (53.85%). Combretum glutinosum alone accounted for 36% of visits. More than half of the melliferous plants (50.94%) were visited for both nectar and pollen. About 32.08% of plants were visited for nectar only (32.08%), while 16.98% were visited for pollen only (16.98%). The majority of savanna plants are flowering in the dry season, but few flowering species can be found throughout the whole year. Savanna woody plant species constitute important food resources for bees, therefore providing a wide range of applications for the development of beekeeping activities in the Sudanian region of West Africa.

scholarly journals In vitro culture of sempre-vivas species (Comanthera): a review

Rodriguésia ◽  
2021 ◽  
Vol 72 ◽  
Author(s):  
Andressa Priscila Piancó Santos Lima ◽  
Alone Lima Brito ◽  
José Raniere Ferreira de Santana
Keyword(s):  
Tissue Culture ◽  
In Vitro Culture ◽  
In Vitro Propagation ◽  
Economic Importance ◽  
In Vitro Conservation ◽  
Future Research ◽  
In Vitro Cultivation ◽  
Natural Form

Abstract The term “sempre-viva” denotes plants whose structures retain their natural form and color after being cut and dried. For these reasons, they are commercially valuable for ornamental purposes. However, due to extractive overexploitation of their inflorescences, some of these species are considered endangered. The genus Comanthera includes the sempre-vivas species with greatest economic importance in Brazil. Previous studies have shown that tissue culture is a workable strategy for in vitro propagation and conservation of species of this genus. However, these studies are still incipient. Therefore, the objective of this review is to summarize the findings on the in vitro cultivation of species of the Comanthera genus, to serve as the basis for future research. The text is structured in two main topics: micropropagation and in vitro conservation.

scholarly journals The use of in vitro culture in dianthus propagation

2013 ◽  
pp. 141-162
Author(s):  
Marija Markovic ◽  
Mihailo Grbic ◽  
Matilda Djukic
Keyword(s):  
Tissue Culture ◽  
In Vitro Culture ◽  
Plant Species ◽  
Ornamental Plants ◽  
Scientific Investigation ◽  
Plant Production ◽  
Commercially Important

Today, in vitro culture is of the great importance in both scientific investigation of under-researched plant species and plant production. In this paper, a review of development and methods of in vitro culture is presented. The main principles are given and the most commonly used methods are described. Special attention was paid to the propagation of Dianthus spp. Tissue culture of commercially important taxa is described in detail, and the review of propagation of other decorative Dianthus spp. that can be used as ornamental plants is also given.

scholarly journals Cerebroside Elicitor Confers Resistance to Fusarium Disease in Various Plant Species

2004 ◽  
Vol 94 (8) ◽  
pp. 813-818 ◽  
Author(s):  
Kenji Umemura ◽  
Shigeki Tanino ◽  
Tadako Nagatsuka ◽  
Jinichiro Koga ◽  
Michiaki Iwata ◽  
...  
Keyword(s):  
Plant Species ◽  
Defense Mechanisms ◽  
Ipomoea Batatas ◽  
Defense Responses ◽  
Control Agent ◽  
Phytopathogenic Fungus ◽  
Tomato Root ◽  
Wide Range ◽  
Root Tissues

In the rice blast fungus pathosystem, cerebroside, a compound categorized as a sphingolipid, was found in our previous study to be a non-racespecific elicitor, which elicits defense responses in rice. Here we describe that cerebroside C is produced in diverse strains of Fusarium oxysporum, a common soilborne agent of wilt disease affecting a wide range of plant species. In addition, some type of cerebroside elicitor involving cerebroside A, B, or C was detected in other soilborne phytopathogens, such as Pythium and Botrytis. Treatment of lettuce (Lactuca sativa), tomato (Lycopersicon esculentum), melon (Cucumis melo), and sweet potato (Ipomoea batatas) with cerebroside B resulted in resistance to infection by each pathogenic strain of F. oxysporum. Induction of pathogenesis-related genes and H2O2 production by treatment with cerebroside B were observed in tomato root tissues. The cerebroside elicitor showed no antifungal activity against F. oxysporum in vitro, indicating that the cerebroside elicitor activates defense mechanisms to confer resistance to Fusarium disease. These results suggest that cerebroside functions as a non-race-specific elicitor in a wide range of plant-phytopathogenic fungus interactions. Additionally, cerebroside elicitor serves as a potential biologically derived control agent.

Sign in / Sign up

Export Citation Format

Share Document