scholarly journals The use of in vitro culture in dianthus propagation

2013 ◽  
pp. 141-162
Author(s):  
Marija Markovic ◽  
Mihailo Grbic ◽  
Matilda Djukic
Keyword(s):  
Tissue Culture ◽  
In Vitro Culture ◽  
Plant Species ◽  
Ornamental Plants ◽  
Scientific Investigation ◽  
Plant Production ◽  
Commercially Important

Today, in vitro culture is of the great importance in both scientific investigation of under-researched plant species and plant production. In this paper, a review of development and methods of in vitro culture is presented. The main principles are given and the most commonly used methods are described. Special attention was paid to the propagation of Dianthus spp. Tissue culture of commercially important taxa is described in detail, and the review of propagation of other decorative Dianthus spp. that can be used as ornamental plants is also given.

scholarly journals PLANT TISSUE CULTURE: A TOOL TO CONSERVE BIODIVERSITY THROUGH RAPID IN VITRO REGENERATION OF DIVERSE PLANT SPECIES

2017 ◽  
pp. 132-139
Author(s):  
Nirali Vora
Keyword(s):  
Tissue Culture ◽  
Plant Species ◽  
Plant Tissue Culture ◽  
Plant Tissue ◽  
In Vitro Regeneration ◽  
Ornamental Plants ◽  
Cost Effective ◽  
Human Consumption ◽  
Natural Forests

Biodiversity is declining with the loss of natural forests across the world. Plant tissue culture is an important biotechnological tool to raise large number of plant species in short spa of time. However, commercial tissue culture laboratories are working on raising plantlets important for human consumption only; which mainly include fruit crops, ornamental plants, timber-yielding forest trees and medicinal plants. There is an urgent need of raising all the different plant species rapidly through tissue culture. Through cultivation of these high yielding and disease-free crops in the forests for consumption of all other species of fauna; conservation of biodiversity can be achieved. However, as tissue culture plantlets are costlier than conventionally raised plants, despite of its advantages its utility is limited. To reduce cost of an important fruit crop, banana during its in vitro regeneration, cost-effective alternatives are proposed.

scholarly journals Ex Vitro Rooting of American Chestnut Improves Acclimatization Survival and Plantlet Quality

2016 ◽  
Vol 34 (3) ◽  
pp. 75-79 ◽  
Author(s):  
Allison D Oakes ◽  
Tyler R. Desmarais ◽  
William A. Powell ◽  
Charles A. Maynard
Keyword(s):  
Tissue Culture ◽  
Keystone Species ◽  
Ex Vitro Rooting ◽  
Plant Production ◽  
Shoot Cultures ◽  
Ex Vitro ◽  
Regulatory Review ◽  
Survival Percentage ◽  
Virus Indexing

Tissue culture of plants has many applications, from producing genetically identical horticultural varieties, to production of secondary metabolites, to virus indexing, and most relevantly, developing novel traits by genetic transformation. Using Agrobacterium-mediated transformation on somatic embryos, blight-resistant American chestnuts [Castanea dentata (Marsh.) Borkh.] have been developed as shoot cultures in plant tissue culture. Rooting tissue-cultured shoots and acclimatizing the rooted plantlets are key steps in tree production. In this study, in vitro and ex vitro rooting methods were compared. The ex vitro method resulted in a lower initial rooting percentage but an overall higher survival percentage, resulting in higher potted plant production. The higher survival was likely due to partial acclimatization taking place before the plantlets were transplanted into potting mix. After 8 weeks, plantlets rooted via the ex vitro method were taller, and had more, and larger, leaves than the in vitro-rooted plantlets. These trees are currently in high demand for inoculation studies for federal regulatory review and eventually for restoration of this keystone species to its native habitat.

scholarly journals Influence of Indole acetic acid and Tryptophan on production of Vinblastine and Vincristine of Catharanthus roseous callus cells in the accumulation media of In Vitro tissue culture

2010 ◽  
Vol 7 (3) ◽  
pp. 1113-1119
Author(s):  
Baghdad Science Journal
Keyword(s):  
Tissue Culture ◽  
High Performance Liquid Chromatography ◽  
Acetic Acid ◽  
Liquid Chromatography ◽  
In Vitro Culture ◽  
High Performance ◽  
Indole Acetic Acid ◽  
Culture Technique

This study on the plant of Ain –AL Bason Catharanthus roseous showed the ability of callus cells that is produced by In Vitro culture technique and transformed to the accumulated media (MS 40gm/L sucrose ,2gm/L IAA Indole acetic acid , 0.5gm/L Tryptophan) to produce Vinblastine and Vincristine compounds. Extraction, purification and quantitive determination of Vinblastine and Vincristine compounds using High performance liquid chromatography technique (HPLC)were carried out. The results showed that the highest concentration of Vinblastine and Vincristine compounds were ( 4.653,12.5 (ppm /0.5 dry Wight respectively from transformed callus cells from MS 40 gm /L sucrose , 2 gm / L NAA Naphthaline acetic acid .

scholarly journals THE COMBINATION OF MURASHIGE AND SKOOG (MS) MEDIA AND ACTIVATED CHARCOAL ON THE GROWTH OF THE Vanda helvola ORCHID PLANT IN VITRO

2021 ◽  
Vol 3 (1) ◽  
Author(s):  
Ata Rofita Wasiati ◽  
Ika Afifah Nugraheni ◽  
Yuli Setiawati
Keyword(s):  
Tissue Culture ◽  
Activated Charcoal ◽  
Ornamental Plants ◽  
Negative Control ◽  
Culture Techniques ◽  
Plant Seeds ◽  
Randomized Design ◽  
Short Period ◽  
The Cost

Orchids of the Vanda helvola genus are extensively grown as ornamental plants. The method of propagation of plant seeds in tissue culture is an alternative that may be employed in the provision of a short period. Murashige and Skoog (MS) media and activated charcoal can optimize orchid cultivation utilizing tissue culture techniques. One of the keys to success in tissue culture is the application of natural types of growing regulatory substances (ZPT) at the proper concentration, which can lower the cost of orchid tissue culture.The objective of this study was to see how a combination of Murashige and Skoog (MS) media and activated charcoal influenced the Vanda helvola orchid subculture in vitro. The researchers employed a Complete Randomized Design (RAL) with four treatments and three repeats: negative control (K), ¼ MS (K1), ½ MS (K2), and 100% MS (K3) (K3). The observed parameters include the percentage of live explant, number of roots, and height of orchid plants.   The results showed that the explant had a 100% life percentage of over 21 HST and had no noticeable influence on the number of roots and the plant's height.

scholarly journals Pre-forcing Treatments Influence Bud Break and Shoot Elongation in Forced Woody Species

1992 ◽  
Vol 10 (2) ◽  
pp. 101-103
Author(s):  
Guochen Yang ◽  
Paul E. Read
Keyword(s):  
In Vitro Culture ◽  
Plant Species ◽  
Woody Plants ◽  
Woody Plant ◽  
Woody Species ◽  
Extended Period ◽  
Shoot Elongation ◽  
Bud Break ◽  
Cold Requirement

Abstract Experiments were conducted to evaluate the feasibility of pre-forcing treatments for the release of bud dormancy of dormant stems of lilac, privet and Vanhoutte spirea. The new softwood growth of these dormant stems was used either as explants for in vitro culture or as cuttings for rooting studies of woody plant species in the off-season. A pre-forcing 15% bleach solution (0.78% NaOCl) soak hastened bud break, enhanced percentage of bud break, and promoted shoot elongation. Pre-forcing wetting agent treatments produced similar results to those of the bleach soak with variation among wetting agents and plant species. Smaller treatment differences were observed in the forcing characteristics when stems were collected later in the winter, probably because the cold requirement of the buds had been completely or partially met. This technique will provide explants for in vitro culture and softwood cuttings for propagation of woody plants over an extended period.

scholarly journals ISOLATION AND CHARACTERIZATION OF MDR BACTERIA FROM IN VITRO CULTURE OF BACOPA MONNIERA AND SUPPLEMENTATION OF NATURAL EXTRACTS TO CONTROL BACTERIAL CONTAMINATION

2016 ◽  
Vol 8 (11) ◽  
pp. 102
Author(s):  
Yash Sharma ◽  
Manish Bhardwaj ◽  
Anshita Nagar ◽  
Neeta Bhagat
Keyword(s):  
Tissue Culture ◽  
In Vitro Culture ◽  
Plant Tissue Culture ◽  
Plant Tissue ◽  
Bacterial Contamination ◽  
Resistant Bacteria ◽  
Bacopa Monniera ◽  
Natural Extracts ◽  
Isolation And Characterization

Objective: The purpose of the present study was to isolate and characterize bacterial contamination from in vitro culture of Bacopa monniera callus culture.Methods: The two selected isolates (1 and 2) were identified by morphological, biochemical and molecular (16S rRNA gene sequencing) methods. Beside this antibiotic resistance was also determined.Results: The isolates were identified as closely related to Enterobacter cloacae (KU350623) (Isolate 1) and Myroides odoratimimus (KU382740) (Isolate 2). The isolate 1 and 2 were found to be resistant to streptomycin (25 mcg), dapsone (10 mcg), erythromycin (15 mcg), chloroamphenicol (25 mcg) and ampicillin (10 mcg). Supplementation of the natural extract was tested to control the contamination due to these multi drug resistant bacteria. Aqueous and alcoholic leaf extracts of Azadirachta indica was added to plant tissue culture media i.e. MS media in order to control contamination.Conclusion: The present studies suggest using natural extracts supplementation as a promising strategy to control the in vitro bacterial contamination in plant tissue culture.

scholarly journals Estimation of Genetic Parameters for in vitro Culture Traits and Selection Best Progenies for Tenera Oil Palm Tissue Culture

2014 ◽  
Vol 47 ◽  
pp. 316-322 ◽  
Author(s):  
Yogo Adhi Nugroho ◽  
I. Made Sumertajaya ◽  
Ni Made Armini Wiendi ◽  
Nurita Toruan-Mathius
Keyword(s):  
Tissue Culture ◽  
In Vitro Culture ◽  
Oil Palm ◽  
Genetic Parameters ◽  
Estimation Of Genetic Parameters

scholarly journals Identification of Stevioside Using Tissue Culture-Derived Stevia (Stevia rebaudiana) Leaves

2015 ◽  
Vol 8s2 ◽  
pp. BCI.S30378 ◽  
Author(s):  
Ziaul Karim ◽  
Daisuke Uesugi ◽  
Noriyuki Nakayama ◽  
M. Monzur Hossain ◽  
Kohji Ishihara ◽  
...  
Keyword(s):  
Tissue Culture ◽  
In Vitro Culture ◽  
Large Scale ◽  
Stevia Rebaudiana ◽  
Potential Method ◽  
Modern Technology ◽  
Commercial Production ◽  
Sugar Levels ◽  
Disease Free

Stevioside is a natural sweetener from Stevia leaf, which is 300 times sweeter than sugar. It helps to reduce blood sugar levels dramatically and thus can be of benefit to diabetic people. Tissue culture is a very potential modern technology that can be used in large-scale disease-free stevia production throughout the year. We successfully produced stevia plant through in vitro culture for identification of stevioside in this experiment. The present study describes a potential method for identification of stevioside from tissue culture-derived stevia leaf. Stevioside in the sample was identified using HPLC by measuring the retention time. The percentage of stevioside content in the leaf samples was found to be 9.6%. This identification method can be used for commercial production and industrialization of stevia through in vitro culture across the world.

scholarly journals INDUKSI DAN REGENERASI KALUS KELADI TIKUS (Typonium flagelliforme. Lodd. ) SECARA IN VITRO

2020 ◽  
Vol 13 (4) ◽  
pp. 142 ◽  
Author(s):  
SITTI FATIMAH SYAHID ◽  
NATALINI NOVA KRISTINA
Keyword(s):  
Tissue Culture ◽  
Genetic Variation ◽  
Carbon Source ◽  
Genetic Variability ◽  
In Vitro Culture ◽  
Basic Medium ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
Group B

<p>ABSTRAK<br />Keladi tikus umumnya diperbanyak secara vegetatif sehingga ragam<br />genetiknya sempit. Penelitian peningkatan keragaman genetik pada keladi<br />tikus melalui kultur in vitro telah dilakukan di Laboratorium Kultur<br />Jaringan, Balai Penelitian Tanaman Obat dan Aromatik (Balittro) Bogor<br />pada bulan April sampai Desember 2005. Bahan tanaman yang digunakan<br />adalah daun steril keladi tikus in vitro. Media dasar yang digunakan adalah<br />Murashige and Skoog (MS) yang diperkaya vitamin dari group B. Sebagai<br />sumber energi digunakan sukrosa sebanyak 30 g/l. Penelitian terdiri dari<br />dua tahap yaitu induksi dan regenerasi kalus. Perlakuan yang diuji pada<br />tahap I adalah beberapa taraf konsentrasi auksin (2,4-D) secara tunggal<br />maupun kombinasi dengan sitokinin (kinetin) terhadap induksi kalus yaitu<br />: 2,4-D 0,1 mg/l; 2,4-D 0,5 mg/l; 2,4-D 1,0 mg/l; 2,4-D 0,1 + kinetin 0,1<br />mg/l; 2,4-D 0,5 mg/l + kinetin 0,1 mg/l; 2,4-D 1.0 mg/l + kinetin 0,1 mg/l;<br />2,4-D 0,1 mg/l + kinetin 0,3 mg/l; 2,4-D 0,5 mg/l +kinetin 0,3 mg/l dan<br />2,4-D 1,0 mg/l + kinetin 0,3 mg/l. Tahap II adalah beberapa taraf<br />konsentrasi benzyl adenin untuk regenerasi kalus. Penelitian disusun<br />menggunakan rancangan acak lengkap dengan pola faktorial dan lima<br />ulangan, dan setiap ulangan terdiri dari satu eksplan. Faktor pertama<br />adalah asal kalus dan faktor kedua adalah beberapa taraf konsentrasi BA<br />yaitu : BA 0,1 mg/l ; BA 0,3 mg/l dan BA 0,5 mg/l. Parameter yang<br />diamati adalah waktu inisiasi kalus, struktur dan warna kalus, jumlah<br />tunas serta penampilan kultur secara visual. Hasil penelitian menunjukkan<br />bahwa kalus asal eksplan daun dapat diinduksi pada perlakuan 2,4-D 1,0<br />mg/l + kinetin 0,1 mg/l dan 2,4-D 1,0 mg/l + kinetin 0,3 mg/l dengan<br />waktu inisiasi 8 sampai 10 minggu setelah perlakuan. Regenerasi kalus<br />terbaik diperoleh pada medium 2,4-D 1,0 mg/l + kinetin 0,3 mg/l<br />mengandung BA 0,3 mg/l dengan rata-rata tunas dan daun yang dihasilkan<br />sebanyak 13,2 tunas dan 4,4 daun.<br />Kata kunci : Keladi tikus, Typonium flagelliforme Lodd., induksi,<br />regenerasi kalus, in vitro</p><p><br />ABSTRACT<br />Induction and regeneration of Rodent tuber calli through<br />in vitro culture<br />Rodent tuber plant (Typonium flagelliforme Lodd) is commonly<br />propagated vegetatively, the repro its genetic variation is narrow. A<br />research to increase the genetic variability of the plant was conducted in<br />Tissue Culture Laboratory of the Indonesian Medicinal and Aromatic<br />Research Institute, Bogor from April to December 2005. The leaf of<br />Rodent tuber in vitro used as an explants. Murashige and Skoog (MS)<br />medium used as basic medium, supplemented with vitamin from B group,<br />sucrose 30 g/l was added into the medium as carbon source. The research<br />consist of two steps : 1) calli induction and 2) calli regeneration. The<br />treatment tested in first step : 2.4-D 0.1 mg/l; 2.4-D 0.5 mg/l; 2.4-D 1,0<br />mg/l; 2.4-D 0.1 + kinetin 0.1 mg/l; 2.4-D 0.5 mg/l + kinetin 0.1 mg/l; 2.4-<br />D 1.0 mg/l + kinetin 0,1 mg/l; 2.4-D 0.1 mg/l + kinetin 0.3 mg/l; 2.4-D 0.5<br />mg/l + kinetin 0.3 mg/l and 2.4-D 1.0 mg/l + kinetin 0.3 mg/l. In the<br />second steps, several concentration of BA were tested i.e: BA 0,1 mg/l ;<br />BA 0,3 mg/l and BA 0,5 mg/l. The experiment was arranged in<br />completely randomized design with factorial pattern. Each treatment<br />consist of five replications. The parameters observed were time of calli<br />initiation, texture, colour of calli and number of shoot and leaves in<br />regeneration. The result showed that calli can be induced on 2.4-D 1.0<br />mg/l + kinetin 0.1 mg/l and 2.4-D 1.0 mg/l + kinetin 0.3 mg/l, eight to ten<br />weeks after culture. The best medium for shoots regeneration contains 2.4-<br />D 1.0 mg/l + kinetin 0.3 mg/l with 0.3 mg/l BA, with mean result of 13.2<br />shoots and 4.4 leaves.<br />Key words : Rodent tuber, Typonium flagelliforme Lodd. bl , induction,<br />regeneration, calli, in vitro</p>

scholarly journals Transient expression of GFP in plant tissue culture in vitro using viral-based module system

1970 ◽  
pp. 198-201
Author(s):  
O. M. Kishchenko ◽  
A. A. Peterson ◽  
M. Yu. Vasylenko ◽  
M. V. Kuchuk
Keyword(s):  
Tissue Culture ◽  
Transient Expression ◽  
Plant Tissue Culture ◽  
Plant Tissue ◽  
Expression System ◽  
Polyacrylamide Gel Electrophoresis ◽  
Plant Production ◽  
Culture In Vitro ◽  
Module System

Aim. Agrobacterium-mediated transient expression using viral-based vectors is one of the most effective method for recombinant protein production in plant. Transient expression of GFP using viral-based module system was studied in plant tissue culture in vitro. Methods. Regenerated shoots and callus clones of Nicotiana benthamiana were agroinfiltrated with viral-based module system. Protein extracts from GFP-positive tissues were resolved by non-reducing polyacrylamide gel electrophoresis. GFP from gel was eluted and GFP fluorescence was measured by fluorescence spectrometry with excitation filter at 395 nm and emission filter at 510 nm. Results. Regenerated shoots and callus clones lines accumulated GFP at 242.0±0.7 and 221.6±4.1 μg per g fresh tissue, respectively. The obtained level of transient expression is comparable with other plant production systems in early stage development. Conclusions. The developed technique shows promise for production of therapeutic proteins and antigens in the short term (14–16 days) by transient expression system in plant tissue culture in vitro. Keywords: viral-based module system, transient expression, recombinant proteins, plant tissue culture, GFP.

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