Effects of Administration of Methanol Root Bark Extract of Cussonia arborea on Serum Biochemical Markers of Liver Damage and Histomorphology of Liver of Alloxan-induced Diabetic Rats

Diabetes mellitus is an endocrine and a metabolic disease resulting from the destruction of pancreatic beta cells; thus assessment of the pancreas in diabetic rats is important in monitoring therapy. The hereby study assessed pancreatic status of diabetic rats treated with methanol root bark extract of Cussonia arborea. A total of seventy two (72) male albino wistar rats weighting between 100-105 g were assigned into six (6) groups of twelve (12) rats per group. Groups 1-5 were diabetic infected by single intraperitoneal injection with alloxan monohydrate, at the dose of 160 mg/kg and treated with 62.5, 125, 250 mg/kg bw of the extract, 2 mg/kg bw glibenclamide and 10 ml/kg distilled water (DW) respectively, while the non diabetic rats, represented by Group 6, received 10 ml/kg DW and served as normal control rats. The treatment was applied daily through the oral route for 84 days. At the end of the experiment, the pancreas organs were acquired under light ether anaesthesia for histomorphometric assessment. The results indicated that the cells of the islet of langerhans of the diabetic untreated rats (Group 5) were severely depleted when compared to that of the normal rats (Group 6). The islet cells of the diabetic rats treated with 125 mg/kg Cussonia arborea extract (Group 2) was comparable to that of the diabetic rats treated with glibenclamide (Group 4) and the normal control rats. It was concluded that the methanol extract of C. arborea, especially at the dose of 125 mg/kg, ameliorated pancreatic lesions induced by diabetes occasioned by alloxan.

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Effect of administration of methanol root bark extract of Cussonia arborea on serum lipid profile and oxidative biomarker parameters in alloxan-induced diabetic rats

Indian Journal of Animal Research ◽

10.18805/ijar.9555 ◽

2016 ◽

Author(s):

Patrick Emeka Aba ◽

Isaac Uzoma Asuzu

Keyword(s):

Oxidative Stress ◽

Total Cholesterol ◽

Serum Lipid ◽

Methanol Extract ◽

Diabetic Rats ◽

Bark Extract ◽

Albino Rats ◽

Root Bark ◽

Oxidative Stress Biomarkers ◽

And Oxidative Stress

This study investigated the effects of methanol extract of Cussonia arborea on serum lipid and oxidative stress biomarkers of alloxan-induced diabetic rats. A total of 72 male albino rats assigned into 6 groups of 12 rats per group were used. Groups 1-5 were made diabetic while group 6 were normal. Groups 1-4 were treated with 62.5, 125, 250 mg/kg of the extract and 2 mg/kg glibenclamide respectively while groups 5 and 6 received 10 ml/kg distilled water each. Total cholesterol, triglyceride, high density lipoprotein (HDL), malondiadehyde, catalase, and superoxide dismutase (SOD) were assayed on days 28, 56 and 84 post treatment. The results indicated that the extract significantly (p<0.05) reduced the levels of total cholesterol, triglyceride, malondialdehyde but significantly (p<0.05) increased the activities of SOD, catalase and the levels of HDL when compared to negative control. It was therefore concluded that methanol extract of C. arborea mitigated dyslipidaemia and oxidative stress.

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Hypoglycemic Activity of Aqueous Root Bark ExtractZanthoxylum chalybeumin Alloxan-Induced Diabetic Rats

Journal of Diabetes Research ◽

10.1155/2016/8727590 ◽

2016 ◽

Vol 2016 ◽

pp. 1-5 ◽

Cited By ~ 3

Author(s):

Moses Solomon Agwaya ◽

Peter California Vuzi ◽

Agnes Masawi Nandutu

Keyword(s):

Blood Glucose ◽

Treatment Options ◽

Diabetic Rats ◽

Bark Extract ◽

Initial Increase ◽

Oral Glucose ◽

Root Extract ◽

Root Bark ◽

Significant Difference ◽

Alloxan Monohydrate

Background. Medicinal plants offer cheaper and safer treatment options to current diabetic drugs. The present study evaluated the effect of aqueous root bark extract ofZanthoxylum chalybeumon oral glucose tolerance and pancreas histopathology in alloxanized rats.Method. Diabetes was induced in rats by administration of alloxan monohydrate. Root extract ofZ. chalybeumwas administered to rats at 200 and 400 mg/kg BW daily for 28 days. Blood glucose was measured by glucometer and pancreatic histopathology evaluated microscopically.Results. Initial increase was observed in blood glucose of the rats after oral administration of glucose from time zero. Two hours after treatment withZ. chalybeum, a significant reduction in blood glucose was observed within treatment groups (p<0.05) compared to 0.5 hr and 1 hr. There was no significant difference between treatment group receiving 400mg/Kg BW extract and the normal groups (p=0.27), implying that the former group recovered and were able to regulate their blood sugar, possibly via uptake of glucose into cells. The reversal in pancreatic histopathology further supports the protective effect ofZ. chalybeumextract towards diabetic damage.Conclusion. Extract ofZ. chalybeumis effective in controlling blood glucose in diabetes and protecting pancreatic tissues from diabetic damage.

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Hypoglycemic, Antioxidant and Hepatoprotective Activities of Ethanolic Root Bark Extract of Chrysophyllum albidum in Alloxan-Induced Diabetic Rats.

Bangladesh Journal of Medical Science ◽

10.3329/bjms.v12i3.12721 ◽

2013 ◽

Vol 12 (3) ◽

pp. 298-304 ◽

Cited By ~ 2

Author(s):

Chika Anna Onyeka ◽

Agnes Akudo Nwakanma ◽

Adeola Airat Bakare ◽

Ini Ibehe Okoko ◽

Uzozie Chikere Ofoego ◽

...

Keyword(s):

Blood Glucose ◽

Fasting Blood Glucose ◽

Medical Science ◽

Diabetic Control ◽

Diabetic Rats ◽

Antidiabetic Activity ◽

Bark Extract ◽

Control Group ◽

Root Bark ◽

Male Wistar Rats

Objective: The study was aimed at investigating the hypoglycemic, antioxidant and Hepatoprotective effects of Chrysophyllum albidum in diabetes induced male Wistar rats. Methods: Ethanol root bark extract was administered to thirty rats of six groups A, B, C, D, E and F of five rats each, weighing between 150-170g. Diabetes was induced in Groups B, C, D, E and F using a single intraperitoneal injection of 140mg/kg of Alloxan after an overnight fast. Group A served as the normal control while Group B served as the diabetic control. Group C had metformin of 500mg while Groups D, E and F received 50, 100 and 200mg/kg / bw/ day of the plant extract respectively through orogastric intubation. All the animals were given normal rat chow and water freely. Blood glucose level was determined and the experiment lasted for 3 weeks. On day 21 after an overnight fast, animal were anaesthetized and blood samples were collected by cardiac puncture under inhaled chloroform for the determination of superoxide dismutase (SOD), catalase (CAT) and malondialdehyde (MDA) assays. The liver function test, ALT, AST and ALP was determined. Result: This showed that C. albidum and metformin significantly (p < 0.05) lowered the Fasting blood glucose (FBS), the activities of SOD and CAT was dose- dependently increase when compared to the diabetic control and there was also a reduction of MDA in the treated groups. There was decrease in the activity of ALT, AST and ALP, which was also dose-dependent. Conclusion: The results showed that the plant has significant antidiabetic activity and could therefore be employed for the treatment of diabetes mellitus in which free radicals are implicated.Bangladesh Journal of Medical Science Vol. 12 No. 03 July 13 Page 298-304 DOI: http://dx.doi.org/10.3329/bjms.v12i3.12721

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Haematological Profile of Alloxan-induced Diabetic Rats Treated with Methanol Root Bark Extract of Cussonia arborea

International Blood Research & Reviews ◽

10.9734/ibrr/2015/22725 ◽

2016 ◽

Vol 4 (4) ◽

pp. 1-10

Author(s):

Patrick Aba ◽

Isaac Asuzu

Keyword(s):

Diabetic Rats ◽

Bark Extract ◽

Root Bark ◽

Haematological Profile

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Effect of Carica papaya L. Stem Bark Extracts on Cholesterol Concentration in Rats Induced with Streptosozin

E3S Web of Conferences ◽

10.1051/e3sconf/202015101011 ◽

2020 ◽

Vol 151 ◽

pp. 01011

Author(s):

Safrida Safrida ◽

Mustafa Sabri

Keyword(s):

Body Weight ◽

Carica Papaya ◽

Stem Bark ◽

Negative Control ◽

Diabetic Rats ◽

Cholesterol Concentration ◽

Bark Extract ◽

Treatment Groups ◽

Randomized Design ◽

Stem Bark Extract

This study was designed to determine the effect of Carica papaya L. stem bark extracts on cholesterol concentration in rats induced with glibenclamide. A completely randomized design was used for the experiment which consisted of 6 treatment groups, each group consisted of four rats, as follows:1) KN (negative control, non-diabetic rats); KP, diabetic rats given glibenclamide 10 mg/kg body weight; EP 1, diabetic rats given 0 mg/kg body weight/day extract; EP2, diabetic rats given 100 mg/kg body weight/day extract; and EP3, diabetic rats given 200 mg/kg body weight/day extract, EP4, diabetic rats given 300 mg/kg body weight/day extract for 28 day. The results showed that C. papaya L. stem bark extract decreased (P<0.05) cholesterol levels in diabetic rats. It was concluded that C. papaya L. stem bark extract had potential as anti-hypercholesterolemic in diabetic rats.

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