A Profoundly Touchy Approved Spectrofluorimetric Approach for Assurance of Ixabepilone as Anticancer Drug by Utilizing Its Quenching Impact on Acetoxymercuric Fluorescein Reagent

2021 ◽  
pp. 39-52
Author(s):  
Hesham Salem ◽  
Amany Abdelaziz ◽  
Aliaa Gamal ◽  
Ramy El Sabaa
Keyword(s):  
Human Plasma ◽  
Anticancer Drug ◽  
Fluorescence Intensity ◽  
Solution Concentration ◽  
Quenching Effect ◽  
Bulk Powder ◽  
Optimized Conditions ◽  
Temperature Time ◽  
Sulfur Containing ◽  
Satisfactory Recovery

A dependable, sensitive, basic and cheap spectrofluorimetric approach has been created for test of sulfur-containing drug; ixabepilone in bulk powder, vials and human plasma. The approach depends on the quenching effect of ixabepilone on the fluorescence intensity of acetoxymercuric fluorescene (AMF) reagent at λem of 530 nm and λex of 500 nm. Parameters which will control the reaction such as pH, AMF solution concentration, temperature, time and solvents were examined and optimized. According to the optimized conditions, the proposed approach was practiced over the concentration area of 20-100 ng mL-1 with adequate linearity (r = 0.9998). The developed approach was approved confirming to ICH rules in terms of accuracy, precision, linearity, LOD and LOQ. The proposed approach was practiced to analyze ixabepilone in Ixempra® vials with satisfactory recovery % of 99.89 and RSE% of 1.24. The results achieved were compared to those achieved by an already reported HPLC approach.

scholarly journals A Study of the Interaction of Ixabepilone As Anticancer Drug With Acetoxymercuric Fluorescein Reagent by Fluorescence Quenching Approach: A Validated Method

2021 ◽  
Author(s):  
Hesham Salem ◽  
Amany abdelaziz ◽  
Aliaa Gamal ◽  
Ramy El Sabaa
Keyword(s):  
Fluorescence Quenching ◽  
Human Plasma ◽  
Solution Concentration ◽  
Quenching Effect ◽  
Accuracy And Precision ◽  
Ich Guidelines ◽  
Sample Extraction ◽  
Temperature Time ◽  
Sulfur Containing

Abstract A spectrofluorimetric approach has been developed and validated for determination of sulfur-containing drug; ixabepilone in raw powder, vials and human plasma. This approach studies the quenching effect of IXA on the fluorescence intensity of acetoxymercuric fluorescein (AMF) reagent at λem of 530 nm and λex of 500 nm. All the parameters that can affect the reaction as pH, AMF solution concentration, temperature, time and solvents were studied and optimized. The linearity range of the studied approach was 20-100 ng mL-1 with correlation coefficient of (r = 0.9998). The proposed approach was validated and approved regarding to ICH guidelines in terms of accuracy, precision, linearity, LOD and LOQ, with mean percentage recovery of 99.79 and RSE% of 1.64. The previously obtained resultes were already statistically compared with that of established reported methods indicating no significant differences in accuracy and precision. Finally, the proposed approach is easy, sensitive, and inexpensive so it is suitable for routine determination of IXA in raw powder, vials and human plasma with no need for any prior separation or sample extraction.

scholarly journals L-Tryptophan as Fluorescent Probe for Determination of Folic Acid in Pharmaceutical Products

2019 ◽  
Vol 7 (2) ◽  
pp. 19-26
Author(s):  
Tara F. Tahir ◽  
Aryan F. Qader ◽  
Musher I. Salih ◽  
Essa Q. Rashid
Keyword(s):  
Quality Control ◽  
Folic Acid ◽  
Fluorescent Probe ◽  
Fluorescence Intensity ◽  
Pharmaceutical Products ◽  
Buffer Solution ◽  
Quenching Effect ◽  
Replicated Measurements ◽  
Optimized Conditions

A new fluorescent probe L-Tryptophan was reported for the determination of folic acid (FA), based on its quenching effect of the fluorescence intensity of L-Tryptophan. The concentration of folic acid was proportional to the quenched fluorescence intensity of L-Tryptophan at an emission wavelength of 365 nm in Britton– Robinson (BR) buffer solution of pH 7. Optimized conditions of pH, time, order of addition of the reagent, potential interferences, concentrations of L-Tryptophan and buffer were investigated. Folic acid was determined in a linear range of 2.0 to 16.0 μg ml-1 with a correlation coefficient R2 0.9974. The limits of detection LOD and quantification LOQ values were 0.09 μg ml-1 and 0.27 μg ml-1, respectively. The standard deviation (RSD) values for five replicated measurements of 2, 8, 16 μg ml-1 folic acid were between 0.23 % and 1.07%. This method is efficient for routine analysis and quality control assay as it is relatively interferences free.

ESTIMATION OF TADALAFIL IN HUMAN PLASMA BY SPECTROFLUOROPHOTOMETRY

INDIAN DRUGS ◽  
2021 ◽  
Vol 58 (06) ◽  
pp. 60-63
Author(s):  
Sadhana Rajput ◽  
◽  
Samir Patel ◽  
Keyword(s):  
Human Plasma ◽  
Fluorescence Spectrum ◽  
Drug Monitoring ◽  
Limit Of Detection ◽  
Wave Length ◽  
Excitation Wavelength ◽  
Limit Of Quantification ◽  
Pharmacokinetic Investigation ◽  
Satisfactory Recovery

A new, specific, selective, simple, rapid and inexpensive spectrofluorophotometric method has been developed for the determination of tadalafil in spiked human plasma. The fluorescence spectrum of tadalafil in 0.1M methanolic sulphuric acid showed excitation wavelength at 315 nm and emission wave-length at 332 nm. The method for tadalafil was found to be linear over the concentration range of 10-50 ng/mL with a correlation coefficient of 0.9991. Limit of detection and limit of quantification were found to be 0.235 ng/mL and 0.701 ng/mL, respectively. The method was validated and found to be suitable for the estimation of tadalafil from human plasma. Satisfactory recovery of tadalafil from the human plasma suggests no interference of any debris present into human plasma. This method can be used to deter-mine plasma tadalafil concentration in drug monitoring or pharmacokinetic investigation.

Label-Free Quantification of Anticancer Drug Imatinib in Human Plasma with Surface Enhanced Raman Spectroscopy

2018 ◽  
Vol 90 (21) ◽  
pp. 12670-12677 ◽  
Author(s):  
Stefano Fornasaro ◽  
Alois Bonifacio ◽  
Elena Marangon ◽  
Mauro Buzzo ◽  
Giuseppe Toffoli ◽  
...  
Keyword(s):  
Raman Spectroscopy ◽  
Human Plasma ◽  
Anticancer Drug ◽  
Surface Enhanced Raman Spectroscopy ◽  
Label Free ◽  
Label Free Quantification ◽  
Surface Enhanced ◽  
Surface Enhanced Raman ◽  
Free Quantification

Effect of Gamma Radiation on Dyeing of Cotton Fabric with Reactive Blue 13

2011 ◽  
Vol 15 (3) ◽  
pp. 107-114 ◽  
Author(s):  
Iram Batool ◽  
Ijaz Ahmad Bhatti ◽  
Shahid Adeel ◽  
Muhammad Abbas
Keyword(s):  
Gamma Radiation ◽  
Gamma Irradiation ◽  
Radiation Treatment ◽  
Reactive Dye ◽  
Iso Standard ◽  
Standard Methods ◽  
Colour Strength ◽  
Dyeing Parameters ◽  
Optimized Conditions ◽  
Temperature Time

The effect of gamma radiation on the dyeing of cotton with Reactive Blue 13 dye powder has been investigated. The cotton and dye powder are irradiated to absorb doses of 100, 200, 300, 400 and 500 Gy by using a Co-60 gamma irradiator. Dyeing is performed by using irradiated and un-irradiated cotton with irradiated and un-irradiated dye powder, in order to observe the effect of radiation treatment on the colour strength of the reactive dye on cotton. Different dyeing parameters such as temperature, time and p H are optimized using irradiated cotton and dye powder. The effect of gamma irradiation on the colour strength and Lab values has been observed through optimized conditions of dyeing. The colour strength and Lab values are obtained by spectra flash spectrophotometer (SF650). ISO standard methods are employed to study the effect of gamma radiation on the Colourfastness properties of fabric. It can be demonstrated that gamma irradiation not only improves the colour strength of the dye in irradiated cotton, but also enhances the Colourfastness properties.

Synthesis and Property of a New Photochromic Diarylethene Bearing Thiophene and a Six-Membered Aryl Unit

2011 ◽  
Vol 197-198 ◽  
pp. 448-451 ◽  
Author(s):  
Hong Liang Liu ◽  
Shou Zhi Pu ◽  
Hui Li ◽  
Gang Liu
Keyword(s):  
Fluorescence Intensity ◽  
Uv Light ◽  
Amorphous Film ◽  
Solution Concentration ◽  
Fluorescent Properties ◽  
Strong Fluorescence ◽  
Photochromic Behavior

A novel photochromic diarylethene based on thiophene and a six-membered aryl moieties 1-[(2-methyl-5-(p-methylphenyl)-3-thienyl)]-2-[(2-trifluoromethylphenyl)]perfluorocyclopentene(1a) has been synthesized. Its properties, including photochromic behavior and fluorescent properties, have been investigated. The results showed that this compound exhibited reversible photochromism, changing from colorless to purple after irradiation with UV light both in solution and in PMMA amorphous film. The fluorescence intensity decreased along with the increase of solution concentration. The diarylethene exhibited relatively strong fluorescence at different solvent.

The Regeneration of the Spent Catalyst Resulted from 3,4,5,6-Tetrachloropyridine-2-Carbonitrile Synthesis

2012 ◽  
Vol 550-553 ◽  
pp. 2436-2446 ◽  
Author(s):  
Yang Li ◽  
Qiu Hong Ai ◽  
Hao Xin Wang ◽  
He’an Luo
Keyword(s):  
Activated Carbon ◽  
Thermal Desorption ◽  
Flow Rate ◽  
Social Benefit ◽  
Process Conditions ◽  
Spent Catalyst ◽  
Economical Benefit ◽  
Iodine Adsorption ◽  
Optimized Conditions ◽  
Temperature Time

In order to optimize the process conditions for regeneration of activated carbon form spent catalyst resulted from 3,4,5,6-tetrachloropyridine-2-carbonitrile synthesis. A two-step process involving the thermal desorption of spent activated carbon and the activation of activated carbon treated by thermal desorption with steam was designed. The influence of desorption temperature, time and the flow rate of nitrogen on the yield of chlorides, the iodine adsorption capacity and the yield of activated carbon were investigated, respectively . The optimal desorption conditions were: temperature of 650°C, time of 90min and the flow rate of nitrogen of 30ml/min. The yield of the chlorides obtained under the optimal conditions was 14.85%. The regeneration of activated carbon treated by thermal desorption with steam; the optimized conditions analyzed by RSM were regeneration temperature of 696°C, time of 84 min and flow rate of steam of 0.58g/min. The experimental results on the optimized conditions were iodine adsorption value of 1003.59mg / g, These methods can not only recover chloride in the spent activated carbon, but also the regeneration activated carbon could partially replace the fresh activated carbon, which would bring an obvious economical benefit and social benefit.

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