scholarly journals Analysis of the Physicochemical and Microbiological Quality of Unfermented Palm Sap Reserved for Infant Feeding

Author(s):  
Voko Bi Rosin Don Rodrigue ◽  
Assohoun-Djeni Nanouman Marina Christell ◽  
Coulibaly Bakary ◽  
Kouassi Kouassi Clément

Aim: The study was conducted to evaluate the physicochemical and microbiological parameters that could contribute to the depreciation of the quality of unfermented palm sap used for infant feeding. Study Design: A survey was conducted in 20 villages and camps to determine if the practice was still current. Also, unfermented sap was produced from 5 palms by resource persons to control the quality during the days of exploitation. Place and Duration of Study: The survey was carried out from March 1st to 30th in villages and camps in Central and Central-Western Cote d'Ivoire. The production of the sap took place during one week in the village of Akpessekro. Methodology: 30 people, without distinction randomly crossed were questioned on the food consumed at the age of 0 to 6 months. Also, in the unfermented sap produced acidity, sugar content, loads of GAM, thermotolerant coliforms, enterococci, yeasts and molds were determined and multiple correlations were established between all parameters. Results: In the villages and camps, palm sap is less and less used as infant food. During the first two days of palm farming, the acidity (6 < pH < 6.11) approximates that of breast milk or milk substitutes. However, after these two days, the acidity becomes high and the sugar content too low for infant feeding. In the unfermented sap, the loads of coliform fecal contamination germs are higher than the required standards. Also, the large load of lactic acid bacteria (≥1.5E+04 ufc/ml) and yeasts (1.4E+04 ufc/ml) present in the sap contribute to a depreciation of its quality over the days and when the sap is left to rest. Conclusion: The quality of unfermented palm sap as infant food is not guaranteed. This is one of the reasons why this practice is in decline nowadays.

2018 ◽  
Vol 2018 ◽  
pp. 1-7 ◽  
Author(s):  
Vanessa Vanderléia Merlini ◽  
Fabíola de Lima Pena ◽  
Diogo Thimoteo da Cunha ◽  
Julicristie Machado de Oliveira ◽  
Mauricio Ariel Rostagno ◽  
...  

The aim of this study was to assess the microbiological profile of leafy vegetables from organic and conventional farming (n=70). The microbiological parameters analyzed consisted of aerobic mesophilic bacteria, yeasts and molds, coliforms at 30 and 45°C, and Salmonella ssp. A biochemical identification of the Enterobacteriaceae species was carried out. Some Enterobacteriaceae species were identified in the produce from both farming systems and Hafnia alvei was the most widespread specie observed. Salmonella spp. was not detected in the samples analyzed. The microbial counts for indicator microorganisms were, in general, higher for conventional leaves when compared to those produced by organic farming.


2009 ◽  
Vol 39 (6) ◽  
pp. 1836-1841 ◽  
Author(s):  
Thaís Mioto Martineli ◽  
Oswaldo Durival Rossi Junior ◽  
Natacha Deboni Cereser ◽  
Marita Vedovelli Cardozo ◽  
Cristianne Lino Fontoura ◽  
...  

The consumption of lamb meat in Brazil has increased in the last years but little information about the microbiological quality of this product is available. To evaluate the hygienic-sanitary conditions of lamb carcasses, the quantification of microorganism populations indicators (mesophiles and psychrotrophs; total and thermotolerant coliforms; Escherichia coli; moulds and yeasts) and the pathogenic microorganisms indentification (Salmonella sp. and Listeria spp.) were performed. A total of 60 lamb carcasses were sampled from one abattoir in São Paulo. Swab samples were collected from three points (forequarter, back and hindquarter) on the muscle surface after carcasses final washing. Statistical analysis consisted of descriptive evaluation of the results whose counts were grouped by intervals of microorganism populations. Counts ranged from 1.0 x 10¹ to 8.0 x 10(4) colony-forming unit cm-2 (CFU cm-2) for mesophiles; 1.0 x 10(0) to 4.4 x 10(4)CFU cm-2 for psychrotrophs; < 1.0 x 10(0) to 4.4 x 10(4)CFU cm-2 for moulds and yeasts; < 0.3 to > 32.0 most probable number/cm² (MPN cm-2) for total and thermotolerant coliforms and Escherichia coli. Salmonella sp. and Listeria spp. were not found in any of the carcasses. Most carcasses presented low counts for all microorganisms. Overall results may be explained by the small size of the industry where the study was taken. Results suggest that good microbiological quality lamb meat is possible to be obtained, but improvement in hygienic-sanitary conditions is still required.


1984 ◽  
Vol 47 (3) ◽  
pp. 177-181 ◽  
Author(s):  
T. G. REHBERGER ◽  
L. A. WILSON ◽  
B. A. GLATZ

A study was done to investigate the microbiological quality of commercial tofu available in local retail outlets. A sampling method was first developed to obtain accurate and representative microbial counts of individual pieces of tofu. Plate count determination of total aerobic organisms, psychrotrophs, coliforms, sporeformers, yeasts and molds, and staphylococci were made on 60 tofu samples (representing three lots each of four different brands) obtained within 24 h after delivery to the retail store. In addition, for two brands that provided manufacturer's pull dates, the same microbial counts were obtained for samples stored in the laboratory at 10°C until the pull date. Of the tofu sampled immediately after purchase, 83% of the lots tested had total counts greater than 106 colony-forming units (CFU)/g and psychrotrophic counts greater than 104 CFU/g. In addition, 67% of the lots tested had confirmed coliform counts greater than 103 CFU/g. Very low levels (less than 10 CFU/g) of all other microbial groups tested for were found in the majority of lots. Samples held until the manufacturer's pull date contained higher total and psychrotrophic counts but lower or stable counts of other organisms compared with samples tested immediately after purchase. To improve the microbiological quality of tofu, processors need to reduce initial loads by improving sanitation and processing techniques, and retailers should provide more consistent and colder refrigerated storage.


Author(s):  
Nesrin Kaval ◽  
Nilgün Öncül ◽  
Zeliha Yıldırım

In this study, the microbiological quality of traditionally produced Bez Sucuk in Tokat and its vicinity was investigated. For this purpose, 30 Bez Sucuk samples obtained from butchers, producers of Bez Sucuk, were analyzed for total count of mesophilic aerobic bacteria, lactic acid bacteria, yeasts-moulds, total coliform, fecal coliform, Staphylococcus aureus, Bacillus cereus, and Cl. perfringens. Also, the presence of Escherichia coli O157:H7, Listeria monocytogenes and Salmonella spp. were investigated in all samples. pH and water activity of the samples were determined. As the result of the analysis, the counts of the microbiological parameters investigated were found as follows: total mesophilic aerobic bacteria 3.5×106-4.23×109 CFU/g, lactic acid bacteria 5.55×105-2.45×109 CFU/g, yeasts and moulds 2.50×103-6.90×109 CFU/g, total coliform


2020 ◽  
Vol 8 (2) ◽  
pp. 185 ◽  
Author(s):  
Charlène Leneveu-Jenvrin ◽  
Baptiste Quentin ◽  
Sophie Assemat ◽  
Mathilde Hoarau ◽  
Jean-Christophe Meile ◽  
...  

Minimally-processed pineapple stored under refrigerated conditions is highly perishable. We aimed to characterize the evolution of physicochemical, sensory and microbiological quality during cold storage. Pineapple batches were sampled from several locations in Reunion Island and then minimally processed. In the processing step, the variability of firmness and counts of yeasts and molds were observed. Moreover, correlations between the sampling season and pH and b* color component, as well as between fungal population and b* parameter were observed. During storage, the visual aspect of pineapple cuts changed to brown and shiny, whereas olfactive descriptors shifted from fruity descriptors and fresh to fermented, alcoholic and milky. The values for pH, TA and TSS did not significantly vary according to storage time. A decrease in firmness and C* color parameter was observed. Yeast and mold counts were significantly higher after 7 days of storage. The diversity in yeasts and molds was mainly dependent on the considered batches observed from PCR-DGGE profiles. Fungal species were isolated from spoiled pineapple cuts. The implication of Penicilllium citrtrinum, Talaromyces amestolkiae, Rhodotorula mucilaginosa, Saccharomyces cerevisiae, and Meyerozyma caribbica in the spoilage of minimally-processed pineapple cuts was further demonstrated.


1996 ◽  
Vol 59 (9) ◽  
pp. 984-987 ◽  
Author(s):  
A. N. MUTUKUMIRA ◽  
S. B. FERESU ◽  
J. A. NARVHUS ◽  
R. K. ABRAHAMSEN

Chemical and microbiological analyses were carried out on 10 samples of raw milk collected over 6 months from the Nharira/Lancashire Milk Collection Center. The milk center is run by smallholder farmers. The purpose of the study was to evaluate the quality of the raw milk delivered to the milk collection center. The average chemical characteristics of the milk were (%): titratable acidity expressed as lactic acid, 0.21; total protein, 3.19; fat, 3.52; total solids, 11.76; and solids not fat, 8.25; the pH varied from 6.15 to 6.65. There were large variations in the microbiological composition of the raw milk with total aerobic counts ranging from 6.2 × 103 to 7.8 × 107 CFU/ml, coli forms from 3.2 × 102 to 2.3 × 105, and lactic acid bacteria from less than 1 × 103 to 2.9 × 106 CFU/ml. Yeasts and molds were less than 100 CFU/ml in 7 of the 10 samples analyzed.


1983 ◽  
Vol 46 (1) ◽  
pp. 34-40 ◽  
Author(s):  
E. C. D. TODD ◽  
G. A. JARVIS ◽  
K. F. WEISS ◽  
G. W. RIEDEL ◽  
S. CHARBONNEAU

Ten types of frozen cream-type pies, manufactured in Canada and imported from the United States, were analyzed for aerobic colony counts, yeasts and molds, coliforms, Escherichia coli, Staphylococcus aureus and Salmonella. The variations in counts depended more on the manufacturer than on the type of pie and the ingredients used. Five of the 465 examined pies had an excess of 105 aerobic colony counts/g, whereas the median value for all the pies examined was between 102 and 103 CFU/g. E. coli and S. aureus were present in few pies, mainly made by one manufacturer, but there was no correlation between high aerobic colony counts and these organisms. Salmonella was not found in any of the pies. Percentage distributions of the estimated ‘population’ of pies available nationally at the time of the survey were statistically determined. These were then compared with suggested national guidelines in the form of a three-class acceptance plan based on United States surveys and desirable manufacturing practices. These indicate that pies should contain aerobic colony counts of &lt;50,000/g, yeast and mold counts of &lt;500/g, S. aureus counts of &lt;100/g, coliform counts of &lt;50/g, E. coli counts of &lt;10/g, and no Salmonella. Three of the six manufacturers would have had an estimated 5.4 to 32.6% of lots in excess of the guidelines at the time of the survey.


2016 ◽  
Vol 79 (5) ◽  
pp. 781-788 ◽  
Author(s):  
JIM McLAUCHLIN ◽  
HEATHER AIRD ◽  
ANDRE CHARLETT ◽  
NICOLA ELVISS ◽  
ANDREW FOX ◽  
...  

ABSTRACT Outbreaks of foodborne illness caused by Bacillus cereus and Listeria monocytogenes in England associated with meat pie consumption were detected in 2012. To obtain baseline data for pies unrelated to outbreaks, 862 samples of ready-to-eat meat pies were collected at retail or from catering facilities in England in 2013 and examined to enumerate food-poisoning bacteria and indicator organisms using Organization for Standardization (ISO) methods for Listeria spp. including L. monocytogenes (ISO 11290), Clostridium perfringens (ISO 21528), coagulase-positive staphylococci including Staphylococcus aureus (ISO 6888), Bacillus spp. including B. cereus (ISO 1737), Escherichia coli (ISO 16649), Enterobacteriaceae (ISO 21528), and aerobic colony counts (ACCs; ISO 4833). Microbiological quality was satisfactory in 94% of samples, borderline in 5%, and unsatisfactory in 1%. The proportion of pies from markets that were borderline or unsatisfactory significantly increased, and the proportion of borderline or unsatisfactory pies from supermarkets significantly decreased. Among the refrigerated (0 to 15°C) pies, microbiological quality significantly decreased in pies stored at &gt;8°C and further significantly decreased at in pies stored at ambient temperature (&gt;15 to 25°C). Samples collected at 25 to 40°C had the highest proportion of borderline or unsatisfactory results, but results improved in pies stored at &gt;40°C. The most common cause for borderline or unsatisfactory results was elevated ACCs (5% of all samples). Within the individual microbiological parameters, borderline or unsatisfactory results resulted from elevated Enterobacteriaceae or Bacillus levels (10 samples for each), C. perfringens levels (2 samples), and S. aureus or E. coli levels (1 sample each). L. monocytogenes was recovered from one pie at &lt;10 CFU/g. A literature review revealed a range of microbiological hazards responsible for food poisoning and meat pie consumption, and surveillance data from 1992 to 2012 from England indicated that C. perfringens was the most commonly reported cause of outbreaks of foodborne illness.


2009 ◽  
Vol 72 (2) ◽  
pp. 421-424 ◽  
Author(s):  
P. L. MOREIRA ◽  
T. B. LOURENÇÃO ◽  
J. P. A. N. PINTO ◽  
V. L. M. RALL

The aim of this work was to assess the microbiological quality of spices sold in Botucatu, São Paulo, Brazil. A total of 233 samples were analyzed for mesophilic bacteria, thermotolerant coliforms, Bacillus cereus, Staphylococcus aureus, and Salmonella. Data showed that 21 and 5.6% of these samples were not in agreement with the standards of Brazilian law, due to an excess of coliforms and to the presence of Salmonella, respectively. Black pepper and cumin exhibited the lowest microbiological quality, whereas bay leaf showed the highest quality. It was concluded that the seasonings possessed poor microbiological quality, and new alternatives should be taken in the primary production in order to improve this quality. Irradiation may also be a tool to assure the safety of these products.


2018 ◽  
Vol 5 (1) ◽  
pp. 138-146
Author(s):  
Ni Luh Nova Dilisca Dwi Putri ◽  
Nyoman Sudarma ◽  
Diah Prihatiningsih

Pendahuluan : Air merupakan sumber daya alam yang dilukan untuk hajat hidup orang banyak (Efendi, 2003). Berdasarkan . Men. Kes. No. 492/MENKES//IV/2010, bahwa air minum adalah air yang melalui proses pengolahan atau tanpa pengolahan memenuhi syarat kesehatan dapat langsung diminum. Desa Kukuh, Kecamatan Marga, Kabupaten Tabanan terbagi menjadi 8 banjar dinas, salah satunya adalah banjar dinas Tatag yang menjadi pusat dalam pemanfaatan sumber mata air minum pada wilayah desa tersebut. Metode: Jenis penelitian yang digunakan adalah penelitian deskriptif tentang uji kelayakan kualitas air berdasarkan parameter kimia dan mikrobiologi dari sumber mata air di Desa Kukuh, Kecamatan Marga, Kabupaten Tabanan. Hasil: Hasil identifikasi kelayakan kualitas air minum yang telah dilakukan sebanyak tiga kali pengujian, yaitu pada bulan (Mei, Juli, September) pada tahun 2018 memberikan hasil: a). Pemeriksaan parameter kimia menunjukkan bahwa seluruh parameter yang diujikan berada pada standar yang dibolehkan, sedangkan b). Pemeriksaan parameter mikrobiologi menunjukkan hasil adanya kandungan bakteri Fecal coli pada sampel mata air dengan jumlah rata-rata 100 Fecal coli/100 mL. Diskusi : Berdasarkan hasil identifikasi pada parameter kimia menunjukkan bahwa hasil identifikasi parameter kimia telah sesuai dengan standar kualitas air minum, sedangkan parameter mikrobiologi belum memenuhi standar kualitas air minum disebabkan adanya kandungan bakteri Fecal coli pada sampel mata air dengan jumlah rata-rata  100 Fecal coli/100 mL. Kata Kunci : Kualitas mata air, Parameter Kimia, Parameter Mikrobiologi.   ABSTRACT Introduction: Water is a natural resource needed for the livelihood of many people. Drinking water is water with or without processing that meet health requirements and can be drunk directly, this is based on . Men Kes No. 492/MENKES//IV/2010. Kukuh Village, Marga District, Tabanan Regency is divided into 8 banjar dinas, one of which is the Banjar Tagtag, which is the center of the utilization of drinking water sources in the village area. Method: The type of research used is a descriptive study of the feasibility of water quality based on chemical and microbiological parameters on water from a spring source in Kukuh Village, Marga District, Tabanan Regency. Results: The results of the identification of the feasibility of drinking water quality that has been carried out three times tn the month of May, July, and September. The results of the identification of chemical and microbiological parameters were  : a). Chemical parameters, showed that all identified parameters are at the missible level. c). Microbiological parameters showed the results of Fecal coli contents in spring samples with and an average number of 100 Fecal coli/100 mL. Discussion: The results of the identification of chemical and microbiological parameters indicated that the identification of chemical parameters were in accordance with drinking water quality standards, while the microbiological parameters did not meet drinking water quality standards, because in the water sample there was the content of Fecal coli bacteria in the spring samples with and average number of 100 Fecal coli/100 mL. Keywords : Quality of Springs, Chemical Parameters, Bacteriological Parameters


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