Improved Efficacy and Stability of Silymarin Loaded Nanocochleates Over Liposomes for the Treatment of Skin Diseases

Aim: Silymarin, a complex polyphenolic component mixture with anti-oxidant, anti-inflammatory, and membrane-stabilizing property is being investigated in several dermatological conditions. Present research aims to evaluate potential of silymarin loaded nanocochleates and liposomal topical application for treating chronic skin diseases. Study Design: Silymarin loaded liposomes and nanocochleates were formulated and optimized using Design Expert software. Different invitro and exvivo tests were performed to compare their performance. Place and Duration of Study: The study was conducted in Smt. Kashibai Navale College of Pharmacy, Pune, India, between January 2019 till February 2020. Methodology: Liposomes were prepared using ethanol injection method and further treated with calcium chloride to form nanocochleates by trapping method. Design of experiments (32 Factorial Design) was used for optimization of nanocochleates. Cell line studies (HaCaT cell lines) and short term stability studies were performed to compare the efficacy and stability respectively. Results: Particle size, entrapment efficiency and drug deposition in Wistar Rat Skin was found to be statistically significant for nanocochleates over liposomes proving superiority of cochleates. Both the carriers sustained release of silymarin for 24h. Antimicrobial efficacy of nanocochleates against E.coli and S.aureus was significant. Inhibition of hyper proliferation of HaCaT cell lines (key mechanism by which most of the antipsoriatic drugs act) demonstrated the superiority of nanocochleates over liposomes.The nanocochleates also displayed better stability compared to liposomes due to decreased entrapment efficacy and leakage of drug. Conclusion: Silymarin loaded Nanocochleates could prove as a promising topical drug delivery system for the treatment of chronic skin diseases like psoriasis.

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The Constructive Thinking Inventory: Factorial Structure in Healthy Individuals and Patients with Chronic Skin Diseases

European Journal of Psychological Assessment ◽

10.1027/1015-5759.14.3.226 ◽

1998 ◽

Vol 14 (3) ◽

pp. 226-233 ◽

Cited By ~ 3

Author(s):

Jürgen Hoyer ◽

Mechthild Averbeck ◽

Thomas Heidenreich ◽

Ulrich Stangier ◽

Karin Pöhlmann ◽

...

Keyword(s):

Skin Diseases ◽

Principal Component ◽

Initial Study ◽

Statistical Independence ◽

Constructive Thinking ◽

Global Factor ◽

Emotional Coping ◽

Factor Congruence ◽

Chronic Skin ◽

Chronic Skin Diseases

Epstein's “Constructive Thinking Inventory” (CTI) was developed to measure the construct of experiential intelligence, which is based on his cognitive-experiential self-theory. Inventory items were generated by sampling naturally occurring automatic cognitions. Using principal component analysis, the findings showed a global factor of coping ability as well as six main factors: Emotional Coping, Behavioral Coping, Categorical Thinking, Personal Superstitious Thinking, Esoteric Thinking, and Naive Optimism. We tested the replicability of this factor structure and the amount of statistical independence (nonredundancy) between these factors in an initial study of German students (Study 1, N = 439) and in a second study of patients with chronic skin disorders (Study 2, N = 187). Factor congruence with the original (American) data was determined using a formula proposed by Schneewind and Cattell (1970) . Our findings show satisfactory factor congruence and statistical independence for Emotional Coping and Esoteric Thinking in both studies, while full replicability or independence could not be found in both for the other factors. Implications for the use and further development of the CTI are discussed.

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167 Influence of Chronic Skin Diseases on Male Sexual Function in Upper Egypt

Journal of Sexual Medicine ◽

10.1016/j.jsxm.2016.12.155 ◽

2017 ◽

Vol 14 (2) ◽

pp. e69

Author(s):

H. Abdel Hafez ◽

A.A. Abdel-Motaleb ◽

H.D. Gaber ◽

G.S.T. Al-Attar

Keyword(s):

Sexual Function ◽

Skin Diseases ◽

Upper Egypt ◽

Male Sexual Function ◽

Chronic Skin ◽

Chronic Skin Diseases

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Quality of Life and Psychosocial Needs of Children Suffering from Chronic Skin Diseases

Alcoholism and psychiatry research Journal on psychiatric research and addictions ◽

10.20471/apr.2016.52.02.04 ◽

2016 ◽

Vol 52 (2) ◽

pp. 133-148 ◽

Cited By ~ 4

Author(s):

Mia Roje ◽

Ines Rezo ◽

Gordana Buljan Flander

Keyword(s):

Quality Of Life ◽

Skin Diseases ◽

Psychosocial Needs ◽

Chronic Skin ◽

Chronic Skin Diseases

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Influência de fatores emocionais nas doenças crônicas de pele: O estresse como gatilho para o desenvolvimento, reincidência ou agravamento da psoríase / Influence of emotional factors on chronic skin diseases: Stress as a trigger for development, recurrence or worsening of psoriasis

ID on line REVISTA DE PSICOLOGIA ◽

10.14295/idonline.v13i46.1914 ◽

2019 ◽

Vol 13 (46) ◽

pp. 584-608

Author(s):

Manuela Sobral Bentes de Melo ◽

Nara Freire Leão Rocha ◽

Stéfany Silva Magalhães ◽

Lais Landim Sousa

Keyword(s):

Skin Diseases ◽

Emotional Factors ◽

Chronic Skin ◽

Chronic Skin Diseases

Diversos estudos têm revelado a existência de uma relação entre aspectos psicossociais e o desenvolvimento ou agravamento de dermatoses crônicas, dentre as quais se encontra a psoríase. Fatores psicológicos, stress e outros eventos da vida são capazes de agravar de 40 e 80% dos casos. O estudo objetivou analisar a forma como esses aspectos podem ensejar o surgimento, a reincidência ou a acentuação do quadro clínico. Metodologia: Estudo de casos concretos com pacientes portadores da psoríase, além de uma vasta literatura bibliográfica acerca da temática. Conclusão: Ficou demonstrada a importância do tratamento psíquico e fisiológico, bem como a necessidade do desenvolvimento de estratégias de coping para enfretamento das situações sociais envolvidas.

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Co(II) Complex of Quercetin–Spectral, Anti-/Pro-Oxidant and Cytotoxic Activity in HaCaT Cell Lines

Applied Sciences ◽

10.3390/app11199244 ◽

2021 ◽

Vol 11 (19) ◽

pp. 9244

Author(s):

Monika Kalinowska ◽

Hanna Lewandowska ◽

Marek Pruszyński ◽

Grzegorz Świderski ◽

Ewelina Gołębiewska ◽

...

Keyword(s):

Antioxidant Activity ◽

Solid State ◽

Cytotoxic Activity ◽

General Formula ◽

Cell Lines ◽

Elemental Analysis ◽

Hacat Cell ◽

Water Solution ◽

Ft Ir ◽

Hacat Cell Lines

In this study a cobalt(II) complex of quercetin was synthetized in the solid state with the general formula Co(C15H9O7)2∙2H2O. The FT-IR, elemental analysis, and UV/Vis methods were used to study the composition of the complex in a solid state and in a water solution. The anti-/pro-oxidant activity of quercetin and the Co(II) complex was studied by means of spectrophotometric DPPH (2,2-diphenyl-1-picrylhydrazyl), FRAP (ferric reducing antioxidant activity) and Trolox oxidation assays. The cytotoxicity of quercetin and Co(II)-quercetin complex in HaCat cell lines was then established.

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Bioactivity Determination of a Therapeutic Recombinant Human Keratinocyte Growth Factor by a Validated Cell-based Bioassay

Molecules ◽

10.3390/molecules24040699 ◽

2019 ◽

Vol 24 (4) ◽

pp. 699 ◽

Cited By ~ 2

Author(s):

Wenrong Yao ◽

Ying Guo ◽

Xi Qin ◽

Lei Yu ◽

Xinchang Shi ◽

...

Keyword(s):

Growth Factor ◽

Cell Lines ◽

Hacat Cell ◽

Keratinocyte Growth Factor ◽

Growth Factor Receptor ◽

Luciferase Reporter ◽

Hematopoietic Stem ◽

Human Keratinocyte ◽

Serum Response ◽

Hacat Cell Lines

The therapeutic recombinant human keratinocyte growth factor 1 (rhKGF-1) was approved by the FDA for oral mucositis resulting from hematopoietic stem cell transplantation for hematological malignancies in 2004. However, no recommended bioassay for rhKGF-1 bioactivity has been recorded in the U.S. Pharmacopoeia. In this study, we developed an rhKGF-1-dependent bioassay for determining rhKGF-1 bioactivity based on HEK293 and HaCat cell lines that stably expressed the luciferase reporter driven by the serum response element (SRE) and human fibroblast growth factor receptor (FGFR2) IIIb. A good responsiveness to rhKGF-1 and rhKGF-2 shared by target HEK293/HaCat cell lines was demonstrated. Our stringent validation was completely focused on specificity, linearity, accuracy, precision, and robustness according to the International Council for Harmonization (ICH) Q2 (R1) guidelines, AAPS/FDA Bioanalytical Workshop and the Chinese Pharmacopoeia. We confirmed the reliability of the method in determining rhKGF bioactivity. The validated method is highly timesaving, sensitive, and simple, and is especially valuable for providing information for quality control during the manufacture, research, and development of therapeutic rhKGF.

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Comparison of Personality Traits among Patients with Psoriasis, Atopic Dermatitis, and Stress: A Pilot Study

Dermatology ◽

10.1159/000505543 ◽

2020 ◽

Vol 236 (4) ◽

pp. 324-328 ◽

Cited By ~ 2

Author(s):

Lynda Grine ◽

Giulia Tochtermann ◽

Hilde Lapeere ◽

Nele Maes ◽

Günther F.L. Hofbauer ◽

...

Keyword(s):

Atopic Dermatitis ◽

Pilot Study ◽

Personality Traits ◽

Skin Diseases ◽

Work Related Stress ◽

Work Related ◽

Chronic Skin ◽

Related Stress ◽

Chronic Skin Diseases ◽

The Impact

Background: Psoriasis and atopic dermatitis are chronic skin diseases that greatly affect the quality of life. Both diseases can be triggered or exacerbated by stress. Objective: We aimed to differentiate personality traits between patients with chronic skin conditions and people treated for stress in a pilot study. Methods: Patients participating voluntarily in educational programs in Belgium and Switzerland were recruited to complete personality trait questionnaires, including the Temperament and Character Inventory (TCI) and the Tridimensional Personality Questionnaire (TPQ). A comparison was made with patients treated for work-related stress. Results: A total of 48 and 91 patients suffering from skin diseases and work-related stress, respectively, were included in the study. Based on the questionnaires, we found that dermatology patients were less persistent and impulsive than those with work-related stress. Dermatology patients also exhibited more rigidness and less focus on performance. Finally, patients with work-related stress seem more likely to change in response to health-promoting programs than patients with chronic dermatoses. Conclusion: Patients with chronic skin diseases may perceive and cope with stress differently in comparison to patients with work-related stress due to inherent personality traits. Therefore, stress coping mechanisms may differ among different diseases. More research is needed into the design of educational interventions and the impact of personality traits in disease-specific groups.

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