scholarly journals Co(II) Complex of Quercetin–Spectral, Anti-/Pro-Oxidant and Cytotoxic Activity in HaCaT Cell Lines

2021 ◽  
Vol 11 (19) ◽  
pp. 9244
Author(s):  
Monika Kalinowska ◽  
Hanna Lewandowska ◽  
Marek Pruszyński ◽  
Grzegorz Świderski ◽  
Ewelina Gołębiewska ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Solid State ◽  
Cytotoxic Activity ◽  
General Formula ◽  
Cell Lines ◽  
Elemental Analysis ◽  
Hacat Cell ◽  
Water Solution ◽  
Ft Ir ◽  
Hacat Cell Lines

In this study a cobalt(II) complex of quercetin was synthetized in the solid state with the general formula Co(C15H9O7)2∙2H2O. The FT-IR, elemental analysis, and UV/Vis methods were used to study the composition of the complex in a solid state and in a water solution. The anti-/pro-oxidant activity of quercetin and the Co(II) complex was studied by means of spectrophotometric DPPH (2,2-diphenyl-1-picrylhydrazyl), FRAP (ferric reducing antioxidant activity) and Trolox oxidation assays. The cytotoxicity of quercetin and Co(II)-quercetin complex in HaCat cell lines was then established.

scholarly journals Effect of Thermal Processing on Antioxidant Activity and Cytotoxicity of Waste Potato Juice

2019 ◽  
Vol 14 (1) ◽  
pp. 150-157 ◽  
Author(s):  
Przemysław Łukasz Kowalczewski ◽  
Anna Olejnik ◽  
Wojciech Białas ◽  
Piotr Kubiak ◽  
Aleksander Siger ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Low Temperature ◽  
Cytotoxic Activity ◽  
Spray Drying ◽  
Cell Lines ◽  
Freeze Drying ◽  
Cytotoxic Activities ◽  
Normal Cells ◽  
Stabilization Methods ◽  
The Impact

AbstractPotato juice (PJ), commonly considered a burdensome waste, is rich in various compounds with bioactive properties. It has long been considered a remedy for gastric problems in traditional folk medicine. If valorization of PJ through implementation in the production of functional foods is to be considered, stabilization methods must be developed to allow long-term storage of this seasonal product. It is important that such methods are chosen with regard to their effect on the bioactive value of the obtained product. In this study, the impact of four stabilization methods on the antioxidant and cytotoxic activities of PJ was investigated. Elevated temperatures were used in thermal deproteinization used to obtain DPJW (deproteinated potato juice water) and spray-drying of FPJ (fresh potato juice) that resulted in SDPJ. Freeze drying and cryoconcentration were the low temperature processing methods that yielded PJL (potato juice lyophilisate) and CPJ (cryocorncentrated potato juice), respectively. All processed materials were characterized chemically and compared with raw materials in terms of phenolic compounds content, antioxidant activity as well as cytotoxicity to human tumor cells isolated from the gastric mucosa (Hs476T cell line), colon (Caco-2 and HT-29 cell lines), and normal cells isolated from the small intestine and colon epithelium (IEC-6 and NCM460 cell lines). It was stated that high-temperature processes – thermal deproteinization and spray-drying – yielded products with increased antioxidant potential (TEAC) that also showed increased cytotoxic activity towards intestinal cancer cells. At the same time the cytotoxicity towards normal cells remained on par with that of fresh PJ (IEC-6 cells) or decreased (NCM460 cells). Thermal deproteinization significantly decreased the content of glycoalcaloids in the juice, while spray drying did not have such an effect. The two low-temperature processes investigated – cryoconcentration and freeze drying – did not affect the PJ cytotoxic activity towards any of the cell lines used in the tests, whereas they did affect the antioxidant properties and glycoalcaloids content of PJ.

scholarly journals Bioactivity Determination of a Therapeutic Recombinant Human Keratinocyte Growth Factor by a Validated Cell-based Bioassay

Molecules ◽  
2019 ◽  
Vol 24 (4) ◽  
pp. 699 ◽  
Author(s):  
Wenrong Yao ◽  
Ying Guo ◽  
Xi Qin ◽  
Lei Yu ◽  
Xinchang Shi ◽  
...  
Keyword(s):  
Growth Factor ◽  
Cell Lines ◽  
Hacat Cell ◽  
Keratinocyte Growth Factor ◽  
Growth Factor Receptor ◽  
Luciferase Reporter ◽  
Hematopoietic Stem ◽  
Human Keratinocyte ◽  
Serum Response ◽  
Hacat Cell Lines

The therapeutic recombinant human keratinocyte growth factor 1 (rhKGF-1) was approved by the FDA for oral mucositis resulting from hematopoietic stem cell transplantation for hematological malignancies in 2004. However, no recommended bioassay for rhKGF-1 bioactivity has been recorded in the U.S. Pharmacopoeia. In this study, we developed an rhKGF-1-dependent bioassay for determining rhKGF-1 bioactivity based on HEK293 and HaCat cell lines that stably expressed the luciferase reporter driven by the serum response element (SRE) and human fibroblast growth factor receptor (FGFR2) IIIb. A good responsiveness to rhKGF-1 and rhKGF-2 shared by target HEK293/HaCat cell lines was demonstrated. Our stringent validation was completely focused on specificity, linearity, accuracy, precision, and robustness according to the International Council for Harmonization (ICH) Q2 (R1) guidelines, AAPS/FDA Bioanalytical Workshop and the Chinese Pharmacopoeia. We confirmed the reliability of the method in determining rhKGF bioactivity. The validated method is highly timesaving, sensitive, and simple, and is especially valuable for providing information for quality control during the manufacture, research, and development of therapeutic rhKGF.

scholarly journals Evaluation of Antimicrobial, Enzyme Inhibitory, Antioxidant and Cytotoxic Activities of Partially Purified Volatile Metabolites of Marine Streptomyces sp.S2A

2018 ◽  
Vol 6 (3) ◽  
pp. 72 ◽  
Author(s):  
Saket Siddharth ◽  
Ravishankar Vittal
Keyword(s):  
Antioxidant Activity ◽  
Cytotoxic Activity ◽  
Cell Lines ◽  
Fungal Pathogens ◽  
Antagonistic Activity ◽  
Gas Chromatography Mass Spectrometry ◽  
Bioactive Metabolites ◽  
Gulf Of Mannar ◽  
Cytotoxic Activities ◽  
16S Rrna Analysis

In the present study, marine actinobacteria Streptomyces sp.S2A was isolated from the Gulf of Mannar, India. Identification was carried out by 16S rRNA analysis. Bioactive metabolites were extracted by solvent extraction method. The metabolites were assayed for antagonistic activity against bacterial and fungal pathogens, inhibition of α-glucosidase and α-amylase enzymes, antioxidant activity and cytotoxic activity against various cell lines. The actinobacterial extract showed significant antagonistic activity against four gram-positive and two gram-negative pathogens. Excellent reduction in the growth of fungal pathogens was also observed. The minimum inhibitory concentration of the partially purified extract (PPE) was determined as 31.25 μg/mL against Klebsiella pneumoniae, 15.62 μg/mL against Staphylococcus epidermidis, Staphylococcus aureus and Bacillus cereus. The lowest MIC was observed against Micrococcus luteus as 7.8 μg/mL. MIC against fungal pathogens was determined as 62.5 μg/mL against Bipolaris maydis and 15.62 μg/mL against Fusarium moniliforme. The α-glucosidase and α-amylase inhibitory potential of the fractions were carried out by microtiter plate method. IC50 value of active fraction for α-glucosidase and α-amylase inhibition was found to be 21.17 μg/mL and 20.46 μg/mL respectively. The antioxidant activity of partially purified extract (PPE) (DPPH, ABTS, FRAP and Metal chelating activity) were observed and were also found to have significant cytotoxic activity against HT-29, MDA and U-87MG cell lines. The compound analysis was performed using gas chromatography-mass spectrometry (GC-MS) and resulted in three constituents; pyrrolo[1–a]pyrazine-1,4-dione,hexahydro-3-(2-methylpropyl)-, being the main component (80%). Overall, the strain possesses a wide spectrum of antimicrobial, enzyme inhibitory, antioxidant and cytotoxic activities which affords the production of significant bioactive metabolites as potential pharmacological agents.

scholarly journals Effect of the Sulfonation on the Swollen State Morphology of Styrenic Cross-Linked Polymers

Polymers ◽  
2020 ◽  
Vol 12 (3) ◽  
pp. 600 ◽  
Author(s):  
Chiara Dalla Valle ◽  
Marco Zecca ◽  
Federico Rastrelli ◽  
Cristina Tubaro ◽  
Paolo Centomo
Keyword(s):  
Solid State ◽  
Elemental Analysis ◽  
Solid State Nmr ◽  
Chlorosulfonic Acid ◽  
Magic Angle Spinning ◽  
Exchange Capacity ◽  
Size Exclusion ◽  
Magic Angle ◽  
Ft Ir ◽  
Polymer Fraction

The chemical structure and morphology of a set of sulfonic gel-type poly(styrene-divinylbenzene) resins (2 mol% DVB) prepared with different synthetic approaches were investigated by solid state NMR, Inverse Size Exclusion Chromatography (ISEC), FT-IR and elemental analysis to compare their swollen state structure. FT-IR and solid state NMR clearly show that the sulfonation mainly occurs in the para- position with respect the main polymer chain. Sensible proportions of sulfone bridges were found in the materials obtained with oleum and chlorosulfonic acid. With oleum, the presence of the sulfone bridges is clearly associated to a reduced ability to swell in the water medium relative to the proton exchange capacity. This highlights the cross-linking action of the sulfone bridges according to ISEC results, showing a high proportion of a dense polymer fraction in the swollen material. An even higher degree of sulfone-bridging, lower swelling ability, and a high proportion of a dense polymer fraction in the swollen material are found in the resin obtained with chlorosulfonic acid. As a matter of fact, Cross Polarization Magic Angle Spinning Nuclear Magnetic Resonance (CP-MAS 13C-NMR), elemental analysis, and ion exchange capacity, show that oleum and chlorosulfonic acid produced resins with remarkably smaller pores and lower swollen gel volume in polar solvents, with respect to concentrated sulfuric acid.

scholarly journals Weighted gene coexpression network and experimental analyses identify lncRNA SPRR2C as a regulator of the IL-22-stimulated HaCaT cell phenotype through the miR-330/STAT1/S100A7 axis

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Meijunzi Luo ◽  
Pan Huang ◽  
Yi Pan ◽  
Zhu Zhu ◽  
Rong Zhou ◽  
...  
Keyword(s):  
Cell Lines ◽  
Hacat Cell ◽  
Differential Expression Analysis ◽  
Coexpression Network ◽  
Cell Phenotype ◽  
Mrna Levels ◽  
Psoriatic Lesion ◽  
Gene Coexpression Network ◽  
Gene Coexpression ◽  
Hacat Cell Lines

AbstractPsoriasis is a chronic inflammatory disease of the skin with highly complex pathogenesis. In this study, we identified lncRNA SPRR2C (small proline-rich protein 2C) as a hub gene with a critical effect on the pathogenesis of psoriasis and response to treatment using both weighted gene coexpression network analysis (WGCNA) and differential expression analysis. SPRR2C expression was significantly upregulated in both psoriatic lesion samples and HaCaT cell lines in response to IL-22 treatment. After SPRR2C knockdown, IL-22-induced suppression of HaCaT proliferation, changes in the KRT5/14/1/10 protein levels, and suppression of the IL-1β, IL-6, and TNF-α mRNA levels were dramatically reversed. In the coexpression network with SPRR2C based on GSE114286, miR-330 was significantly negatively correlated with SPRR2C, while STAT1 and S100A7 were positively correlated with SPRR2C. By binding to miR-330, SPRR2C competed with STAT1 and S100A7 to counteract miR-330-mediated suppression of STAT1 and S100A7. MiR-330 overexpression also reversed the IL-22-induced changes in HaCaT cell lines; in response to IL-22 treatment, miR-330 inhibition significantly attenuated the effects of SPRR2C knockdown. STAT1 and S100A7 expression was significantly upregulated in psoriatic lesion samples. The expression of miR-330 had a negative correlation with the expression of SPRR2C, while the expression of SPRR2C had a positive correlation with the expression of STAT1 and S100A7. Thus, SPRR2C modulates the IL-22-stimulated HaCaT cell phenotype through the miR-330/STAT1/S100A7 axis. WGCNA might uncover additional biological pathways that are crucial in the pathogenesis and response to the treatment of psoriasis.

Regulation of Human Beta-Defensin 3(hBD-3) in Human Keratinocyte(HaCaT) Cell Lines

2003 ◽  
Vol 15 (1) ◽  
pp. 1 ◽  
Author(s):  
Yu-Jin Kim ◽  
Chang-Kwun Hong ◽  
Seong-Jun Seo
Keyword(s):  
Cell Lines ◽  
Hacat Cell ◽  
Human Keratinocyte ◽  
Hacat Cell Lines

TOPICAL ANTI-PSORIATIC NANOPARTICULATE DRUG DELIVERY SYSTEM

2020 ◽  
pp. 76-85
Author(s):  
KIRAN BHISE ◽  
SHADAB KHAN ◽  
GHAZALA MULLA
Keyword(s):  
Nitric Oxide ◽  
Drug Delivery ◽  
Lipid Peroxidation ◽  
Zeta Potential ◽  
Cell Lines ◽  
Hacat Cell ◽  
In Vitro Diffusion ◽  
Ultraviolet Ray ◽  
Hacat Cell Lines

Objective: Development of effective drug delivery in the treatment of psoriasis is the major challenge for its successful management. To develop and assess the potential of Nanostructured Lipid Carriers (NLCs) enriched with the powdered leaves extracts of Azadirachta indica (AE), Lawsonia inermis (LE) and fruit extract of Mallotus philippensis (ME) in the management of psoriasis. Methods: Drug loaded NLCs were prepared via hot homogenization technique by adopting 23 factorial design with factors X1 as the concentration of lipids, X2 concentration of surfactants and X3 being the number of homogenization cycle. The responses Y1 and Y2 were particle size and zeta potential. The optimized batch was obtained from Surface response plot and was evaluated for zeta potential, % entrapment efficiency, % drug loading, Scanning Electron Microscopy(SEM), % in vitro diffusion of drugs from the NLCs, anti-lipid peroxidation and nitric oxide scavenging activities, cytotoxicity on HaCat cell lines, Mouse Tail and Rat ultraviolet ray B photodermatitis models for Psoriasis. Results: The optimized batch of NLCs was found within the nanosized range with a relatively low polydispersity index and zeta potential of-20mV. The %EE for an optimized batch of NLCs was found to be 98.97±0.83%, 96.99±0.56% and 99.25±0.55% and the %DL of 21.84±0.15%, 8.55±045%, and 87.91±0.38% respectively for AE, LE and ME. The SEM images showed the spherical vesicular structures of drugs loaded NLCs. The in-vitro diffusion of drugs from the NLCs followed initial burst release thereafter sustained release for 24 h. The AE, LE and ME loaded NLCs proved to possess anti-lipid peroxidation and nitric oxide scavenging activities, cytotoxicity on HaCat cell lines, DNA fragmentation on HaCat cell lines which are biomarkers in the pathogenesis of psoriasis. The results of Mouse Tail and Rat ultraviolet ray B photodermatitis models for Psoriasis supported the anti-psoriatic potential of AE, LE and ME loaded NLCs. Conclusion: AE, LE and ME loaded NLCs can be used for prolonged topical delivery to the psoriatic skin for an effective treatment.

scholarly journals Synthesis, characterisation and reactions of cationic complexes of Arylantimony (III) chlorides ArnSbCl3-n with perchlorate and tetrafluoroborate anions

2013 ◽  
Vol 1 (2) ◽  
pp. 42-48
Author(s):  
Ram Nath Prasad Yadav
Keyword(s):  
Molecular Weight ◽  
Solid State ◽  
General Formula ◽  
1H Nmr ◽  
Elemental Analysis ◽  
Chemical Data ◽  
Physico Chemical ◽  
Hexamethyl Phosphoramide

Several hitherto unreported cationic complexes of the general formula [Ar2SbL][Y] and [ArSbL2][Y]2 [ Where, Ar  =  C6H5, L =  α-Picoline, Pyridine, Ph3AsO, hexamethyl phosphoramide (HMPA), thiourea (TU) and Y = ClO4− BF4− ] have been synthesized and characterized by solid state IR, 1H NMR, elemental analysis, conductance and molecular weight measurements. The physico-chemical data, cations [Ar2SbL]+1 and[ArSbL2]+2  are assigned a pyramidal structure.DOI: http://dx.doi.org/10.3126/ijasbt.v1i2.8204 Int J Appl Sci Biotechnol, Vol. 1(2): 42-49

Sign in / Sign up

Export Citation Format

Share Document