Effect of Remineralisation with Nano-hydroxyapatite (nHAp) and Non-collagenous Protein Analogues (PAA and STPP) on the Micro Tensile Bond Strength of the Resin Composites to Dentin – An In vitro Study

Background: Resin-dentin bonds are less durable than enamel as they rely on organic part for mineralisation. Bond denaturation mainly takes place because of hydrolysis of polymerized resin or degradation of water-rich, resin-sparse collagen by collagenolytic endogenous matrix metalloproteinase and cysteine cathepsins. Hence, this limited stability of dentin bonding tenaciously reduces the longevity of tooth-coloured restorations. One of the strategies adopted to encounter this shortcoming is to induce biomimetic remineralisation. Aim: To assess the microtensile bond strength of resin composites to dentin following remineralisation using an experimental paste containing nano-hydroxyapatite (nHAp) and biomimetic analogues, Poly Acrylic Acid (PAA) and Sodium Tri Polyphosphate (STPP). Methodology: Nine intact freshly extracted third molar were used for this study to evaluate the microtensile bond strength after treating with three different groups; Group I with paste containing nano hydroxyapatite nHAp Group II paste containing nHAp and non-collagenous protein analogues such as (Polyacrcrylic acid and Sodium tripolyphosphate) and the control groups containing adhesive and resin composite with no additional treatment. The samples were observed over a period of 24 hours and 1 week the inter group data analysis was done using ANOVA and paired sample t test and the intra-group data analysis over period of 24 hours the post HOC Bonferroni test was used for the statistical analysis. Results: After 24 hours the group with nHAp group showed highest value for microtensile bond strength among other groups, however after one week the group with nHAp +NCP analogues(PAA+STPP) showed highest value for microtensile bond. Conclusion: The treatment with the experimental paste [nHAp+NCP] analogues (PAA+STPP)] can bring about remineralisation to improve bonding to dentin.

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The Effect of Energy Application Sequence on the Microtensile Bond Strength of Different C-factor Cavity Preparations

Operative Dentistry ◽

10.2341/06-30 ◽

2007 ◽

Vol 32 (2) ◽

pp. 124-132 ◽

Cited By ~ 6

Author(s):

G. Maghaireh ◽

M. R. Bouschlicher ◽

F. Qian ◽

S. R. Armstrong

Keyword(s):

Bond Strength ◽

Clinical Relevance ◽

High Irradiance ◽

Resin Composites ◽

Microtensile Bond Strength ◽

Light Curing ◽

Bond Strengths ◽

Energy Application

Clinical Relevance Light curing of resin composites in high C-factor cavity preparations using a high irradiance energy application sequence may lead to decreased bond strength. However, the high irradiance energy application sequence did not result in lower bond strengths in lower C-factor cavity preparations.

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An in vitro Evaluation of Microtensile Bond Strength of Resin-based Sealer with Dentin Treated with Diode and Nd:YAG Laser

The Journal of Contemporary Dental Practice ◽

10.5005/jp-journals-10024-1297 ◽

2013 ◽

Vol 14 (2) ◽

pp. 183-187 ◽

Cited By ~ 4

Author(s):

G Anil Kumar ◽

Maneesha Das ◽

Sindhu Ramesh ◽

Surendranath Garapati

Keyword(s):

Bond Strength ◽

Nd:Yag Laser ◽

Root Canal ◽

Smear Layer ◽

Control Group ◽

Microtensile Bond Strength ◽

Group Iii ◽

Group I ◽

Ah Plus

ABSTRACT Background Smear layer is a negative factor which prevents adhesion of the filling material to the dentinal walls. Recent advances in dental research have incorporated lasers as a potential adjunct in root canal treatment by removing the smear layer before filling the root canal system, enhancing the adhesion of sealers to dentin and improving the sealing ability. Aim To evaluate the microtensile bond strength of AH-Plus resin-based sealer to dentin after treatment with 980 nm diode and 1,064 nm neodymium-doped:yttrium aluminum garnet (Nd:YAG) laser in vitro. Materials and methods Thirty specimens prepared for three groups namely group I (control), group II (980 nm diode–lased specimens) and group III (Nd:YAG–lased specimens). One tooth from each group was observed under scanning electron microscope for evaluation of intracanal root dentin morphology. Remaining specimens were used for making microsections by hard tissue microtome. Specimens for groups II and III were lased with 980 nm diode and 1,064 nm Nd:YAG laser. AH Plus sealer was applied onto specimens and mounted onto Instron universal testing machine for microtensile bond strength testing. Results were subjected to statistical analysis using one-way analysis of variance (ANOVA) and Tukey's test. Results Group III Nd:YAG had maximum mean microtensile bond strength values (11.558 ± 0.869), followed by group II diode (9.073 ± 0.468) and group I control (6.05 ± 0.036). Statistically significant differences were seen among all the groups. SEM analysis shows removal of smear layer in both groups II and III. Conclusion Both Nd:YAG and diode laser were more effective than control group in improving the microtensile bond strength of AH Plus sealer to dentin. Clinical significance Lasers have the potential to increase the adhesiveness of root canal sealer to dentin surface, thereby improving the quality of root canal obturation. How to cite this article Maneesha D, Anil KG, Sindhu R, Surendranath G, Deepak S. An in vitro Evaluation of Microtensile Bond Strength of Resin-based Sealer with Dentin Treated with Diode and Nd:YAG Laser. J Contemp Dent Pract 2013;14(2): 183-187.

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Low- and high-viscosity bulk-fill resin composites: a comparison of microhardness, microtensile bond strength, and fracture strength in restored molars

Acta Odontológica Latinoamericana ◽

10.54589/aol.34/2/173 ◽

2021 ◽

Vol 34 (2) ◽

pp. 173-182

Author(s):

Fabiana França ◽

Jonathan Tenuti ◽

Isabela Broglio ◽

Lara Paiva ◽

Roberta Basting ◽

...

Keyword(s):

Bond Strength ◽

Fracture Strength ◽

High Viscosity ◽

Fracture Pattern ◽

Resin Composites ◽

Microtensile Bond Strength ◽

Thermomechanical Cycling ◽

Low Viscosity ◽

Significant Difference ◽

Knoop Microhardness

The aim of this study was to compare low- and high-viscosity bulk-fill composites for Knoop microhardness (KHN), microtensile bond strength (MTBS) to dentin in occlusal cavities, and fracture strength (FS) in molars with mesialocclusal-distal restoration. Disk-shaped samples with different thicknesses (2 or 4 mm) of low-viscosity (SDR Flow, Dentsply) and high-viscosity bulk-fill composites (Filtek BulkFill, 3M ESPE; and Tetric-N Ceram Bulk Fill, Ivoclar Vivadent) were prepared for top and bottom KHN analysis (n=10). MTBS to dentin and fracture pattern was evaluated in human molars with occlusal cavities restored with (n=10): conventional nanocomposite (Z350XT, 3M ESPE), low-viscosity (Filtek Bulk-fill Flow, 3M ESPE) or high-viscosity bulk-fill composites (Filtek BulkFill). The FS and fracture pattern of human molar with mesial-occlusal-distal restorations submitted or not to thermomechanical cycling were investigated (n=10) using: intact tooth (control), and restoration based on conventional microhybrid composite (Z250, 3M ESPE), low-viscosity (SDR Flow) or high-viscosity bulk-fill composites (Filtek BulkFill). The data were submitted to split-plot ANOVA (KHN), one-way ANOVA (MTBS), two-way ANOVA (FS) followed by Tukey’s test (α=0.05). For KHN, there was no significant difference for the resin composites between the top and bottom. For MTBS, no significant differences among the materials were detected; however, the low-viscosity composite presented lower frequency of adhesive failures. For FS, there was no significant difference between composites and intact tooth regardless of thermomechanical cycling. Low- and high-viscosity bulk-fill composites have comparable microhardness and microtensile bond strength when used in occlusal restorations. Likewise, the bulk-fill composites present similar fracture strength in molars with mesio-occlusal-distal restorations.

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Comparative Evaluation of Different Antioxidants on Reversal of Microtensile Bond Strength of Composite Resin in Endodontically Treated Tooth Surface

JOURNAL FOR CLINICAL AND DIAGNOSTIC RESEARCH ◽

10.7860/jcdr/2021/46654.14936 ◽

2021 ◽

Author(s):

Ramesh Bharti ◽

Anil Chandra

Keyword(s):

Bond Strength ◽

Composite Resin ◽

Testing Machine ◽

Alpha Tocopherol ◽

Tooth Surface ◽

Control Group ◽

Pulp Chamber ◽

Microtensile Bond Strength ◽

Group V ◽

Group I

Introduction: The use of Sodium Hypochlorite (NaOCl) as an endodontic irrigant changes the properties of dentine and lowers the bond strength of resin cements to dentine. But it can be reversed by the use of antioxidants. Aim: To evaluate the effect of different antioxidants for restoring adhesion potential/reversal of microtensile bond strength of dentin of the pulp chamber treated with NaOCl and Ethylenediaminetetracetic Acid (EDTA). Materials and Methods: It was a laboratory based experimentalin-vitro study in which 40 freshly extracted human incisors were selected and cut to expose the pulp chamber dentin. The specimens were distributed into five groups: Group I samples were irrigated with 5.25% NaOCl for 30 minutes followed by 17% EDTA for 3 minutes and final rinse was done with 5.25% NaOCl (Positive control). Group II samples were treated with distilled water only (Negative control). In Group III, samples were treated same as in group I followed by 10% Sodium Ascorbate (SA) for 10 minute. Samples of group IV samples were also treated as in group I followed by application of 5% solution of Alpha tocopherol with for 10 minutes. Group V samples were treated same as in group I followed by rinse with 5% solution of Sodium Thiosulphate (Na2S2O3) for 10 minutes. Composite resin build up was performed and interface was tested by using Universal testing machine for Microtensile bond strength. The resulting data was analysed by one-way Analysis of Variance (ANOVA) and Mann-Whitney U test (p<0.05). Results: The results demonstrated that irrigation with 5.25% NaOCl and 17% EDTA produce significant (p<0.05) reduction in resin dentin bond strength but this can be reversed by application of 5% Na2S2O3. Highest bond strength was observed in group V (22.38±0.84) and lowest for group I (15.38±1.22). Conclusion: Dentin bond strength was significantly reduced when bonding was performed immediately after use of NaOCl. Use of antioxidants after final irrigation with NaOCl can be recommended for better bonding of composite resin.

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Comparison of bond strength between self-adhered and conventional composites

Padjadjaran Journal of Dentistry ◽

10.24198/pjd.vol22no3.15805 ◽

2018 ◽

Vol 22 (3) ◽

Author(s):

Adria Permana Putra ◽

Elin Karlina ◽

Renny Febrida

Keyword(s):

Bond Strength ◽

Testing Machine ◽

Third Molar ◽

Critical Factor ◽

Test Method ◽

The Self ◽

Laboratory Research ◽

Cylindrical Shape ◽

Group I ◽

Strength Difference

Bond strength towards dentine is a critical factor for composite restoration success. The purpose of this research was to obtain data on self-adhered composite bond strength to tooth dentine. This research was an experimental laboratory research that used 10 third molar teeth as samples. The teeth were prepared by thinning its mesial part until it reaches dentine layer. The sample was divided into two groups, each consisting of 5 teeth. In group I was applied self-adhered composite with Fusio® Liquid Dentine brand, whereas group II was applied conventional composite with Ivoclar Te-Econom Flow®. The applied composite had a cylindrical shape with 6 mm diameter. The sample was then placed on the test arm. The shear bond strength test method then conducted on Universal Testing Machine (UTM). Obtained data was tested using Two-tails Independent t-Test (α = 0,05). The results showed the average of self-adhered composite bond strength to dentine is 1,8878 ± 0,2859 MPa and conventional composite for 4,43552 ± 0,49472 MPa. The data showed that the self-adhered composite bond strength to dentine is smaller than conventional composite. From this research can be concluded that there was bond strength difference between the self-adhered composite with conventional composites towards the tooth dentine.

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Comparison of the Microtensile Bond Strength Of Four Different Bulk-Fill Resin Composites Of Class I Cavities With Dentin

Cumhuriyet Dental Journal ◽

10.7126/cumudj.598724 ◽

2019 ◽

pp. 336-344

Author(s):

SERRA KUTLU ◽

İHSAN HUBBEZOĞLU

Keyword(s):

Bond Strength ◽

Class I ◽

Resin Composites ◽

Microtensile Bond Strength

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Effect of Casein Phosphopeptide Amorphous Calcium Phosphate Conditioning on Microtensile Bond Strength of Three Adhesive Systems to Deep Dentin

Frontiers in Dentistry ◽

10.18502/fid.v17i34.5198 ◽

2021 ◽

Author(s):

Mohammad Ali Mohammadi Torkani ◽

Sepideh Mesbahi ◽

Amir Ardalan Abdollahi

Keyword(s):

Calcium Phosphate ◽

Bond Strength ◽

Amorphous Calcium Phosphate ◽

In Vitro Study ◽

Adhesive Systems ◽

Microtensile Bond Strength ◽

Significant Difference ◽

Bonferroni Test ◽

Casein Phosphopeptide

Objectives: This study aimed to assess the effect of casein phosphopeptide amorphous calcium phosphate (CPP-ACP) on microtensile bond strength of three adhesive systems to deep dentin. Materials and Methods: In this in vitro study, the occlusal surface of 30 sound human third molars was sectioned at 2 mm below and above the cementoenamel junction. The samples were randomly divided into three groups of OptiBond Solo Plus, G-Bond, and Clearfil SE Bond. Before composite resin bonding, each group was divided into two subgroups with and without dentin preparation with CPP-ACP. The teeth were then sectioned into 1 mm rods (n=15), and their microtensile bond strength was measured at a crosshead speed of 0.5 mm/min. Data were analyzed using two-way ANOVA and post-hoc Bonferroni test. Results: There was no significant difference in bond strength of OptiBond Solo Plus (P=0.44) and Clearfil SE Bond (P=0.67) with/without CPP-ACP. A significant difference was found between the two subgroups of G-Bond in this respect (P<0.001). The differences in microtensile bond strength among all subgroups were significant (P<0.05). Conclusion: The microtensile bond strength in use of G-Bond is significantly higher following dentin treatment with CPP-ACP compared with no use of CPP-ACP.

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Bond strength of one-step self-etch adhesives and their predecessors to ground versus unground enamel

European Journal of Dentistry ◽

10.1055/s-0039-1698962 ◽

2012 ◽

Vol 06 (03) ◽

pp. 280-286 ◽

Cited By ~ 5

Author(s):

A. Ruya Yazici ◽

Zeren Yildirim ◽

Atila Ertan ◽

Gül Ozgunaltay ◽

Berrin Dayangac ◽

...

Keyword(s):

Bond Strength ◽

Shear Bond Strength ◽

Testing Machine ◽

Third Molar ◽

Bonding Performance ◽

Etch And Rinse ◽

One Step ◽

Bonferroni Test ◽

Bond Strengths ◽

Self Etch

ABSTRACTObjectives: The aim of this study was to compare the shear bond strength of several self-etch adhesives to their two-step predecessors to ground and unground enamel.Methods: Seventy-five extracted, non-carious human third molar teeth were selected for this study. The buccal surfaces of each tooth were mechanically ground to obtain flat enamel surfaces (ground enamel), while the lingual surfaces were left intact (unground enamel). The teeth were randomly divided into five groups according to the adhesive systems (n=15): one-step self-etch adhesive - Clearfil S3 Bond, its two-step predecessor - Clearfil SE Bond, one-step self-etch adhesive - AdheSE One, and its two-step predecessor - AdheSE, and a two-step etch-and-rinse adhesive - Adper Single Bond 2(control). After application of the adhesives to the buccal and lingual enamel surfaces of each tooth, a cylindrical capsule filled with a hybrid composite resin (TPH) was seated against the surfaces. The specimens were stored in distilled water at 37°C for 24 hours, followed by thermocycling (5°C-55°C/500 cycles). They were subjected to shear bond strength test in a universal testing machine at a crosshead speed of 1.0 mm/minute. The data were compared using a two-way ANOVA, followed by Bonferroni test at P<.05Results: All adhesives exhibited statistically similar bond strengths to ground and unground enamel except for the etch-and-rinse adhesive that showed significantly higher bond strengths than the self-etch adhesives (P<.05). No significant differences in bond strength values were observed between ground and unground enamel for any of the adhesives tested (P=.17).Conclusions:Similar bond strengths to ground and unground enamel were achieved with one-step self-etch adhesives and their predecessors. Enamel preparation did not influence the bonding performance of the adhesives tested. (Eur J Dent 2012;6:280-286)

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Evaluating Resin-Dentin Bond by Microtensile Bond Strength Test: Effects of Various Resin Composites and Placement Techniques

The Open Dentistry Journal ◽

10.2174/1874210601509010409 ◽

2015 ◽

Vol 9 (1) ◽

pp. 409-413 ◽

Cited By ~ 1

Author(s):

Horieh Moosavi ◽

Fatemeh Maleknejad ◽

Maryam Forghani ◽

Elham Afshari

Keyword(s):

Bond Strength ◽

Resin Composite ◽

Strength Test ◽

Class I ◽

Resin Composites ◽

Microtensile Bond Strength ◽

Significant Difference ◽

Filling Method ◽

Bulk Insertion

Objectives : This in vitro study evaluated the microtensile bond strength (MTBS) of a methacrylate- based compared to a silorane-based resin composite in Class I cavity using different placement techniques.Materials and Methods: Class I cavities with dimension of (4 mm long, 4 mm wide, 3 mm deep) were prepared in extracted sound human molars. The teeth were randomly divided into six groups. The first three groups were filled with Filtek P90 using three methods of insertion; bulk, incremental and snow-plow, and the remaining three groups were filled with Clearfil AP-X using the same three placement techniques. After 24 hours of storage in water at 37°C, the specimens were thermocycled to 1000 cycles. Specimens were prepared for MTBS testing by creating bonded beams obtained from the pulpal floor. Statistical analysis used: Statistical analyses of data were performed by two-way ANOVA/Tukey (α=.05). Results: The experiment showed significant differences between the two resin composites with regard to filling techniques (P<0.05). The MTBS was significantly higher in each of Filtek P90 subgroup compared to Clearfil AP-X ones (P<0.05). With respect to filling technique in both resin composites, bulk insertion showed the significantly lowest MTBS (P<0.05), while no significant difference was found between the outcome of incremental and snow-plow techniques (P>0.05). Conclusion: Silorane-based resin composite as opposed to methacrylate based resin composite and layering placements in contrast to bulk filling method had higher microtensile bond strength.

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