scholarly journals Antimicrobial Susceptibility Pattern and Molecular Identification of Acinetobacter baumannii in Alex Ekwueme-Federal University Teaching Hospital Abakaliki, Nigeria

2021 ◽  
pp. 409-419
Author(s):  
Ikechukwu Herbert Egwu ◽  
Ifeanyichukwu Romanus Iroha ◽  
Modesta Mmaduabuchi Egwu-Ikechukwu ◽  
Ikemesit Udeme Peter ◽  
Charity Chinyere Nnabugwu ◽  
...  
Keyword(s):  
Multidrug Resistance ◽  
16S Rrna ◽  
Acinetobacter Baumannii ◽  
Teaching Hospital ◽  
Antimicrobial Susceptibility ◽  
Opportunistic Pathogen ◽  
University Teaching ◽  
Clinical Samples ◽  
Acinetobacter Species ◽  
Catheter Urine

Background and Objectives: Acinetobacter baumannii, a notorious opportunistic pathogen known to seriously affect debilitated individuals especially intensive care unit (ICU) patients and others with underlying illness, have consistently jeopardized many antibiotics. This study was therefore aimed to ascertain the antimicrobial susceptibility profile and molecularly identify A. baumannii pathogens in Alex Ekwueme-Federal University Teaching Hospital, Abakaliki, Nigeria. Methodology: A total of 385 clinical samples were collected aseptically from debilitated patients and analyzed following standard microbiological procedures. Acinetobacter species was confirmed by Gram staining reaction and biochemical tests. All bacterial isolates were phenotypically screened for multidrug resistance using the Kirby–Bauer disc diffusion technique and results interpreted as per CLSI criteria. A. baumannii isolates were finally confirmed using 16S rRNA sequencing. Results: A total of 23(6%) A. baumannii isolates were recovered from 385 clinical samples collected from 87 patients comprising 48 males and 39 females admitted in various hospital wards of AE-FETHA. The age of the patients varied from 20–79 years. The commonest sites for isolation of A. baumannii pathogen were catheter urine (8/8%) and wound sores (7/8%). The highest percentage resistance was observed with cefuroxime (96%), tetracycline (96%), sulfamethoxazole/trimethoprim (96 %), and ofloxacin (91%) while meropenem (91%) and imipenem (78%) were the most effective antibiotics against A. baumannii. The isolated A. baumannii was re-confirmed genotypically by 16S rRNA gene amplification. Variations were observed in the gene sequence of all the isolated A. baumannii.  Conclusion: Catheter urine, wound sores, and respiratory fluids were the more easily colonized samples. Also, high frequency of multidrug resistance observed in this study further established A. baumannii as a notorious opportunistic pathogen.

scholarly journals Antimicrobial Susceptibility Pattern of Acinetobacter calcoaceticus-Acinetobacter baumannii Complex Isolated from Sputum in a Tertiary Care Hospital

2019 ◽  
Vol 41 (3) ◽  
pp. 59-62
Author(s):  
Jyotshna Sapkota ◽  
Manisha Sharma ◽  
Deepti Shrestha ◽  
Beena Jha
Keyword(s):  
Multidrug Resistance ◽  
Acinetobacter Baumannii ◽  
Tertiary Care ◽  
Acinetobacter Calcoaceticus ◽  
Clinical Microbiology Laboratory ◽  
Care Hospital ◽  
Sputum Specimen ◽  
Susceptibility Pattern ◽  
Acinetobacter Species ◽  
Acinetobacter Baumannii Complex

Introduction Acinetobacter calcoaceticus-Acinetobacter baumanni (ACB) complex is one of the commonest cause of hospital acquired and ventilator associated pneumonia. Multidrug resistant Acinetobacter species have become a matter of huge concern. This study was done to find out the antibiotic susceptibility pattern of Acinetobacter calcoaceticus-Acinetobacter baumanii complex from sputum samples. MethodsThis descriptive cross-sectional study was carried out in Clinical Microbiology laboratory from July 2018 to Jan 2019 after ethical approval. Acinetobacter calcoaceticus-Acinetobacter baumannii complex was identified on the basis of its microscopy and morphological characteristics followed by biochemical tests. Antibiotic sensitivity test of isolated pathogens was done using Muller Hinton Agar by Kirby-Bauer method. ResultsOf the 384 culture positive sputum specimen, 76 (19.80%) were Acinetobacter calcoaceticus-Acinetobacter baumannii complex. Most of the isolates were resistant to commonly used antibiotics, 72.36% of the isolates were multidrug resistance and 3.95% isolates were resistant to tigecycline. ConclusionThis study provides valuable information regarding prevalence of Acinetobacter calcoaceticus-Acinetobacter baumannii complex from sputum specimen. The alarming number of Multidrug resistance isolates is worrisome finding. Antibiotics like Tigecycline and Colistin which is still sensitive to isolates should be cautiously used only in MDR cases.

scholarly journals Urinary Tract Infection Due to Non-fermentative Gram-Negative Bacilli in Tribhuvan University Teaching Hospital, Kathmandu, Nepal

NUTA Journal ◽  
2020 ◽  
Vol 7 (1-2) ◽  
pp. 71-78
Author(s):  
Santosh Kumar Yadav ◽  
Uday Kant Jha ◽  
Jeevan Bahadur Sherchan
Keyword(s):  
Urinary Tract ◽  
Teaching Hospital ◽  
Antibiotic Susceptibility ◽  
Hospitalized Patients ◽  
The Body ◽  
University Teaching ◽  
Burkholderia Cepacia Complex ◽  
Acinetobacter Species ◽  
Gram Negative ◽  
Tract Infections

Urinary tract infections (UTIs) are the second most common type of bacterial infection of the body affecting humans throughout their lifetime. They are a frequent cause of nosocomial in fection in many hospitals. Therefore, this study was designed to isolate and identify the non-fermentative Gram-negative bacilli (NFGNB) causing UTI in hospitalized patients and determine their antibiotic susceptibility profile. This study was carried in Tribhuvan University Teaching Hospital, Nepal among hospitalized patients. The urine sample was cultured and the antibiotic susceptibility profile of isolated NFGNB was determined by standard microbiological procedures. Among the total of 49 NFGNB isolates, Acinetobacter species (n=21, 42.9%) and Pseudomonas aeruginosa (n=21, 42.9%) were the major isolates and the remaining was Burkholderia cepacia complex (n=7, 14.3%). These isolates were found resistant to commonly used antibiotics. From the present study, it is clear that NFGNB are an important bacterial pathogen capable of producing UTI in hospitalized patients.

Occurrence of Non-Fermenting Gram-Negative Bacilli and Their In Vitro Susceptibility Pattern by Vitek 2 at a Tertiary Care Teaching Hospital – An Observational Study

2021 ◽  
Vol 8 (8) ◽  
pp. 429-434
Author(s):  
Atit Dineshchandra Shah ◽  
Urvashi Natubhai Limbachia ◽  
Bhavin K. Prajapati ◽  
Lata Patel ◽  
Dharati Tusharbhai Shah ◽  
...  
Keyword(s):  
Drug Resistance ◽  
Multidrug Resistance ◽  
Antimicrobial Susceptibility ◽  
Susceptibility Testing ◽  
Tertiary Care ◽  
Carbapenem Resistance ◽  
Antimicrobial Susceptibility Testing ◽  
Clinical Samples ◽  
Gram Negative ◽  
Vitek 2

BACKGROUND Non fermenting gram-negative bacilli (NFGNB) are a group of heterogenous, aerobic and non-sporing saprophytic bacteria, found as commensals in humans and other animals primarily causing opportunistic healthcare-associated infections. They are innately resistant to many antibiotics and are known to acquire resistance by various mechanisms. They pose a particular difficulty for the healthcare community because multidrug resistance is common and increasing among them and a number of strains have now been identified that exhibit pan drug resistance. This study was conducted to isolate and identify various non-fermenter gram negative bacilli (NFGNB), to study their antibiotic sensitivity pattern and their clinical significance from various clinical samples. METHODS A study was undertaken from March 2019 to February 2020 to isolate NFGNB from various clinical samples received for culture and sensitivity in the department of microbiology in a tertiary care hospital, Ahmedabad. Non lactose fermenting colonies on MacConkey agar plates were further processed by Vitek 2 to identify them and to study their antimicrobial susceptibility testing (AST). RESULTS A total of 2010 NFGNB were isolated from various clinical samples and their AST was evaluated by Vitek 2. Pseudomonas aeruginosa (52.7 %) and Acinetobacter baumannii (36.5 %) were the most common NFGNB isolated. Carbapenem resistance was 93 % for Acinetobacter species and 61 % for Pseudomonas species. CONCLUSIONS Accurate and rapid identification and antimicrobial susceptibility testing of NFGNB help in early initiation of appropriate antimicrobial therapy and proper management of patients thereby help in reducing emergence of MDR strains of NFGNB, mortality and overall hospital stay. KEYWORDS NFGNB – Non-Fermenting Gram-Negative Bacilli, Multidrug Resistance, Pan Drug Resistance, Carbapenem Resistance

scholarly journals Identification, Speciation and Antibiogram along with Detection of Metallo Beta-lactamase Production in Acinetobacter Isolated from Clinical Samples in a Tertiary Care Hospital

2021 ◽  
Author(s):  
N Ashwin Chitrabanu ◽  
Shrikara Mallya
Keyword(s):  
Acinetobacter Baumannii ◽  
Tertiary Care ◽  
Antibiotic Sensitivity ◽  
Clinical Samples ◽  
Care Hospital ◽  
Acinetobacter Species ◽  
Carbapenem Resistant ◽  
Appropriate Treatment ◽  
Sensitivity Pattern ◽  
Synergy Test

Acinetobacter species are gram negative non fermenters, which are important nosocomial pathogens involved in various outbreaks in hospitals due to widespread resistance to majority antibiotics. The aim of this study is to speciate Acinetobacter isolated from clinical samples, to assess the antibiotic sensitivity pattern and to detect the production of metallo-β-lactamase by double disc synergy test. The study was conducted in the department of microbiology, A. J Institute of Medical Sciences. All clinical samples were subjected to gram stain & cultured; the Acinetobacter isolates obtained were subjected to antibiogram. Those isolates that showed Imipenem resistant were further tested for production of metallo-β-lactamase by double disc synergy test. Out of 6625 culture positive isolates, 414 (36.1%) were identified biochemically to belong to Acinetobacter species. Of the 414 cases, 393 (94.9%) were further identified to be Acinetobacter baumannii and the remaining 21 (5.1%) to be Acinetobacter lwoffi. Acinetobacter lwoffii showed 100% sensitivity to all the drugs. Of the 393 Acinetobacter baumannii isolates 109 (27.7%) showed resistant to Imipenem. Out of these 109 isolates, 65 (59.63%) were positive for metallo-β-lactamase production by double disk synergy test. The speciation is highly demanding and laborious but it’s important to be demonstrated due to difference in the antibiotic susceptibility pattern. Carbapenem resistant Acinetobacter nosocomial strains in ICUs are detected to be more resistant to antibiotics. As shown in this study the metallo-β-lactamase producing A.baumannii isolates were 59.63% and therapeutic options were limited. Therefore early identification of metallo-β-lactamase producers is of great importance to start appropriate treatment and to control the spread.

scholarly journals Circulation of imipenem-resistant Acinetobacter baumannii ST10, ST2 and ST3 in a university teaching hospital from Tehran, Iran

2019 ◽  
Vol 68 (6) ◽  
pp. 860-865
Author(s):  
Soha Seyyedi Abhari ◽  
Farzad Badmasti ◽  
Leila Modiri ◽  
Mohamad Mehdi Aslani ◽  
Mehdi Asmar
Keyword(s):  
Acinetobacter Baumannii ◽  
Teaching Hospital ◽  
University Teaching
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