scholarly journals Antimicrobial Activity of Cymbopogon (Lemongrass) on Enterococci

2021 ◽  
pp. 684-690
Author(s):  
A. Sai Sanjith ◽  
N. P. Muralidharan
Keyword(s):  
Antimicrobial Activity ◽  
Diffusion Method ◽  
Dilution Method ◽  
Disc Diffusion ◽  
Zone Of Inhibition ◽  
Disc Diffusion Method ◽  
Antibiotic Susceptibility Test ◽  
Lemongrass Oil ◽  
Test Organisms ◽  
Broth Dilution

Aim: The aim of this study was to investigate antimicrobial activity against Enterococcus bacteria. Materials and Methods: Minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were determined by the Broth Dilution Method. The antibiotic susceptibility test against the test organisms was performed by the Disc Diffusion Method. Results and Discussion: Antimicrobial activity of Cymbopogon oil was examined against common Enterococcus bacteria using broth microdilution assay and the disc diffusion method. Zone of inhibition was seen when Cymbopogon oil was kept with enterococcus bacteria, this proves to be bacteriostatic. Conclusion: Lemongrass oil proved to be bacteriostatic against Enterococcus, further research to be done to implement Lemongrass oil as an antibacterial agent.

scholarly journals The Composition and Antimicrobial Activity of the Essential Oil of Teucrium Stocksianum Subsp. Stocksianum Leaf from Oman

2006 ◽  
Vol 1 (3) ◽  
pp. 1934578X0600100 ◽  
Author(s):  
Abdulkhader Hisham ◽  
Nirmal Pathare ◽  
Salim Al-Saidi
Keyword(s):  
Antimicrobial Activity ◽  
Essential Oil ◽  
Diffusion Method ◽  
Disc Diffusion ◽  
Disc Diffusion Method ◽  
Minimal Inhibitory Concentrations ◽  
Streptococcus Species ◽  
Teucrium Stocksianum ◽  
Test Organisms ◽  
Total Oil

The essential oil of Teucrium stocksianum Boiss. subsp. stocksianum leaf grown in Oman was analyzed by GC-MS. Forty seven components amounting to 76.1% of the total oil were identified. The major constituents were α-cadinol (7.6%), β-selinene (6.4%), trans-verbenol (5.9%), caryophyllene oxide (5.7%), α-phellandren-8-ol (5.0%), verbenone (5.0%) and δ-cadinene (5.1%). The antimicrobial activity of the oil was tested against a panel of seventeen bacterial and six fungal strains by the disc diffusion method. The oil inhibited the growth of all test organisms at various levels; however, it showed significant inhibitory activity against Staphylococcus and Streptococcus species, as well as yeasts. The minimal inhibitory concentrations (MIC) were also determined.

scholarly journals Gayatri Mantra Chanting Helps Generate Higher Antimicrobial Activity of Yagya’s Smoke

2018 ◽  
Vol 1 (1) ◽  
pp. 09-14 ◽  
Author(s):  
Ruchi Singh ◽  
Sunil Kumar Singh
Keyword(s):  
Escherichia Coli ◽  
Antimicrobial Activity ◽  
Inhalation Therapy ◽  
Diffusion Method ◽  
Disc Diffusion ◽  
Human Pathogens ◽  
Zone Of Inhibition ◽  
Disc Diffusion Method ◽  
Plant Materials

Yagya (known as a fire-ritual) is an ancient Vedic Indian method of herbal inhalation therapy. It is also described as an excellent mode of environmental purification. Here, a comparative study of antimicrobial activity of smoke extract of Yagya and non-Yagya has been performed. ‘Yagya’ in this text, refers to the burning of wood, specific types of dried medicinal plant materials (hawan samagri) along with ghee with the citation of mantras, whereas the term Non-Yagya implies burning of wood, hawan samagri, along with ghee. In non-Yagya the mantra citation has not been done. An apparatus was designed to simulate the burning process, and the smoke fraction was captured for antimicrobial activity on human pathogens i.e Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa, Bacillus subtilis, and Salmonella typh. Antimicrobial activity was performed using disc diffusion method. Antimicrobial data revealed that the ‘smoke-extract’ obtained from Yagya experiments with mantra chanting had higher zone of inhibition values compared to that of same experiment conditions except presence of mantra chanting suggesting an important role of the mantra chanting in Yagya for medicinal applications

scholarly journals ANTIMICROBIAL EFFICACY OF CARABAO GRASS LEAVES ON STAPHYLOCOCCUS AUREUS

2019 ◽  
Vol 7 (1) ◽  
pp. 384-397
Author(s):  
Dani Aiko Perral Garduque ◽  
Kyrie Radiance Ganal Mateo ◽  
Shalom Moyinoluwa Adejumoke Oyinloye ◽  
Jo Anne kristine Lalas Lucero
Keyword(s):  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
The Philippines ◽  
Antimicrobial Efficacy ◽  
Diffusion Method ◽  
Disc Diffusion ◽  
Zone Of Inhibition ◽  
Disc Diffusion Method ◽  
Treatment And Prevention ◽  
Testing Center

Antimicrobial Efficacy of Carabao Grass (Paspalum conjugatum) leaves on Staphylococcus aureus Dani Aiko Garduque1, Kyrie Radiance Mateo1, Shalom Moyinoluwa Oyinloye1, and Jo Anne Kristine Lalas-Lucero2   1Student, Adventist University of the Philippines 2Faculty, Adventist University of the Philippines   [email protected]   Abstract   Introduction: In the Philippines, the Paspalum conjugatum or known by its common name as “carabao grass” is usually consumed for livestock as well as for landscaping. Paspalum conjugatum has been known to have phytochemicals that kill microorganisms. This experimental research determined the antimicrobial efficacy of Paspalum conjugatum against Staphylococcus aureus.   Methods: Samples of the carabao grass was purchased, washed and then dried. An extract was obtained from the sample. The extract was then sent to an accredited testing center for phytochemical test to identify the different components of the plant extract. The antimicrobial activity of the paspalum conjugatum extract was then determined through the use of the disc diffusion method. The zone of inhibition of Paspalum Conjugatum was also compared to a control standard such as oxacillin.   Results: The results of the study revealed to have abundant constituents of sterols, flavonoids and tannins; moderate levels of triterpenes, saponins and glycosides; and traces of alkaloids. The antimicrobial testing showed results that Paspalum Conjugatum had 10mm zone of inhibition which means that it has a complete inhibitory activity with severe reactivity against the tested organism, staphylococcus aureus.   Discussion: The study concludes that Paspalum conjugatum can be used to eliminate staphylococcus aureus. It is recommended that further studies be conducted for the use of Paspalum conjugatum in the treatment and prevention of diseases caused by staphylococcus aureus. Keywords:  paspalum conjugatum, carabao grass, staphylococcus aureus  

scholarly journals Nasal Carriage of Methicillin Resistant Staphylococcus aureus Among Health Care Workers At Tertiary Care Hospital In Dhaka.

2017 ◽  
Vol 11 (2) ◽  
pp. 17-19
Author(s):  
Rahima Akter ◽  
Shikha Paul ◽  
Akhtarun Naher ◽  
Moshiur Rahman ◽  
Sharmin Sultana ◽  
...  
Keyword(s):  
Tertiary Care ◽  
Nasal Carriage ◽  
Healthcare Personnel ◽  
Diffusion Method ◽  
Resistance Pattern ◽  
Dilution Method ◽  
Care Hospital ◽  
Disc Diffusion ◽  
Disc Diffusion Method ◽  
Mannitol Salt Agar

Nasal carriage of MRSA among hospital stuff act as a source of endogenous infection and becomes a source for hospital and community acquired infection. The study was conducted to determine the rate antibiotic resistance pattern of nasal carriage of MRSA among the hospital stuff of Sir Salimullah Medical College and Mitford Hospital, Dhaka. Pre moistened nasal swabs from hospital stuff (doctor, nurses, lab technicians and other helping stuff were obtained. These swabs were inoculated into Blood agar and Mannitol salt agar media. Antibiogram was done by modified Kirby Bauer disc diffusion method. MRSA were detected by oxacillin and cefoxitin disc diffusion method. The resistance was confirmed by MIC of oxacillin agar dilution method. Out of 142 samples 34 strains of Staphylococcus were isolated among them 07 (4.93%) were MRSA and 27 (19.01%) were MSSA. The carriage rate of MRSA was higher among nurse than other healthcare provider. Nasal carriage of MRSA is responsible for spreading infection from healthcare personnel to normal individual. So, regular screening of carrier is required from prevention of hospital acquired infection. Bangladesh J Med Microbiol 2017; 11 (2): 17-19

scholarly journals EVALUATION OF ANTIBACTERIAL ACTIVITY OF A CYANOBACTERIUM SCYTONEMA SCHMIDTII GOM

2021 ◽  
pp. 77-79
Author(s):  
Janardhan Namdeo Nehul
Keyword(s):  
Escherichia Coli ◽  
Antibacterial Activity ◽  
Methanol Extract ◽  
Axenic Culture ◽  
Soil Samples ◽  
Diffusion Method ◽  
Disc Diffusion ◽  
Zone Of Inhibition ◽  
Disc Diffusion Method ◽  
Air Blower

Scytonema schmidtii,a cyanobacterium was isolated from the collected soil samples from different locations of Ahmednagar district of Maharashtra state (India). Identification was carried out using morphological variation and taxonomical approaches according to Desikachary (1959) and Prescott (1962) .The axenic culture of Scytonema schmidtii was obtained by using the method recommended by Bolch and Blackburn (1996). The isolated Scytonema schmidtii was grown autotropically in BG-11 medium as described by Rippka et al.,(1979) and incubated at 30±2°c.After 25 days, biomass was harvested by filtration through double layered muslin cloth and dried using air blower. The biomass of this Scytonema schmidtii species was used for the assessment of antibacterial activity against Escherichia coli, Bacillus subtilis, Staphylococcus aureus, Pseudomonas aeruginosa, Micrococcus flavus and Proteus mirabilis. The antibacterial activity was studied by disc diffusion method (Anonymous,1996).Methanol extract of Scytonema schmidtii showed the activity against all the tested bacterial strains.Maximum zone of inhibition (23±1.9 mm) was recorded with methanol extract of Scytonema schmidtii.

scholarly journals Synthesis and Antimicrobial Activity of Piperine Analogues Containing 1,2,4-Triazole Ring

2019 ◽  
Vol 31 (5) ◽  
pp. 1077-1080
Author(s):  
Kottakki Naveen Kumar ◽  
Karteek Rao Amperayani ◽  
V. Ravi Sankar Ummdi ◽  
Uma Devi Parimi
Keyword(s):  
Antimicrobial Activity ◽  
Triazole Ring ◽  
Diffusion Method ◽  
Moderate Activity ◽  
Disc Diffusion ◽  
Disc Diffusion Method ◽  
Antibacterial Study ◽  
Gram Negative ◽  
E Coli ◽  
Micro Organisms

A series 1,2,4-triazole piperine analogues (TP1-TP6) were designed and synthesized. The structures were confirmed using 1H NMR and 13C NMR. Antibacterial study was done using Gram-positive (Staphylococcus aureus and Bacillus cereus) and Gram-negative micro-organisms (E. coli and Pseudomonas aeruginosa) by disc diffusion method. Compound containing chloro substitution (TP6) showed the highest effect, while compound TP1, TP3, TP4, TP5 showed the moderate activity.

scholarly journals Chemical Composition and Antibacterial Activity of Essential Oil of Anvillea radiata Coss. & Dur

2007 ◽  
Vol 2 (5) ◽  
pp. 1934578X0700200
Author(s):  
Fadwa El Hanbali ◽  
Ahmed El Hakmaoui ◽  
Fouad Mellouki ◽  
Lahoussine El Rhaffari ◽  
Mohamed Akssira
Keyword(s):  
Antimicrobial Activity ◽  
Antibacterial Activity ◽  
Chemical Composition ◽  
Essential Oil ◽  
Diffusion Method ◽  
Disc Diffusion ◽  
Disc Diffusion Method ◽  
Aerial Parts

The essential oil of the aerial parts of Anvillea radiata Coss. & Dur. was examined by GC-MS. Twenty-nine constituents were identified, representing 88.8% of the total. 6-Oxocyclonerolidol (66.6%) and 6-hydroxycyclonerolidol (11.4%) were found to be the major components. The disc diffusion method, used for antimicrobial activity testing, showed that the essential oil had a significant antibacterial activity against all tested microorganisms.

scholarly journals Synthesis and Antimicrobial Assessment of Fe3+ Inclusion Complex of p-tert-Butylcalix[4]arene Diamide Derivative

2019 ◽  
Vol 2019 ◽  
pp. 1-8 ◽  
Author(s):  
Anwar Ali Chandio ◽  
Ayaz Ali Memon ◽  
Shahabuddin Memon ◽  
Fakhar N. Memon ◽  
Qadeer Khan Panhwar ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Diffusion Method ◽  
Gram Negative Bacteria ◽  
Disc Diffusion ◽  
Disc Diffusion Method ◽  
Gram Negative ◽  
Mueller Hinton ◽  
Mueller Hinton Agar ◽  
Ft Ir ◽  
Bacteria And Fungi

Present study deals with the synthesis of the p-tert-butylcalix[4]arene diamide derivative as ligand (L) and its Fe3+ complex, followed by its characterization using TLC and FT-IR, while UV-Vis and Job’s plot study were performed for complex formation. Antimicrobial activity of the derivative (L) and its metal complex was carried out by the disc diffusion method against bacteria (Escherichia coli and Staphylococcus albus) and fungi (R. stolonifer). Different concentrations of the derivative (L) (6, 3, 1.5, 0.75, and 0.37 μg/mL) and its Fe3+ complex were prepared, and Mueller–Hinton agar was used as the medium for the growth of microorganisms. Six successive dilutions of the derivative (L) and Fe3+ complex were used against microorganisms. Two successive dilutions (6 and 3 μg/mL) of the derivative (L) showed antibacterial action against both Gram-positive and Gram-negative bacteria. In addition, three successive dilutions (6, 3, and 1.5 μg/mL) of the derivative (L) showed antifungal activity. However, all of six dilutions of the Fe3+ complex showed antimicrobial activity. Derivative (L) showed 3 and 1.5 μg/mL minimum inhibitory concentrations (MIC) against bacteria and fungi, respectively. On the contrary, its Fe3+ complex showed 0.37 μg/mL value of MIC against bacteria and fungi. Hence, Fe3+ complex of the derivative (L) was found to be a more effective antimicrobial agent against selected bacteria and fungi than the diamide derivative (L).

scholarly journals Synthesis and Antimicrobial Activity ofSome New 2-Substituted Benzothiazole Derivatives

2009 ◽  
Vol 6 (3) ◽  
pp. 775-779 ◽  
Author(s):  
B. Rajeeva ◽  
N. Srinivasulu ◽  
S. M. Shantakumar
Keyword(s):  
Antimicrobial Activity ◽  
Spectral Data ◽  
Diffusion Method ◽  
Disc Diffusion ◽  
Phosphoryl Chloride ◽  
Mass Spectral Data ◽  
Disc Diffusion Method ◽  
Mass Spectral ◽  
Benzothiazole Derivatives ◽  
H Nmr

Some new 2-(5-substituted-1,3,4-oxadiazole-2-yl)-1,3- benzothiazole(3a-j)were synthesized by refluxing benzothiazolyl carboxyhydrazide with different aryl acids in phosphoryl chloride. Structures of the synthesized compounds were established on the basis of1H NMR and Mass spectral data. The anti microbial activity of the synthesized compounds was evaluated by disc diffusion method.

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