Dynamic Protein Adsorption-Desorption Analysis of Contact Lenses in a Three-Dimensional-Printed Eye Model

Author(s):  
Wan-Hsin Chang ◽  
Pei-Yi Liu ◽  
Dai-En Lin ◽  
Yuan-Ting Jiang ◽  
Chien-Ju Lu ◽  
...  
2014 ◽  
Vol 69 (6) ◽  
pp. 1334-1343 ◽  
Author(s):  
Shasha Lu ◽  
Ruijie Li ◽  
Xiaoming Xia ◽  
Jun Zheng

Measuring pollutant concentrations in major tributaries is the standard method for establishing pollutant fluxes to the sea. However, this method is costly and difficult, and may be subject to a great deal of uncertainty due to the presence of unknown sources. This uncertainty presents challenges to managers and scientists in reducing contaminant discharges to water bodies. As one less costly method, a three-dimensional model was developed and used to predict pollutant fluxes to the sea. The sorptive contaminant model was incorporated into hydrodynamic and sediment models. Adsorption–desorption of copper by sediments in the Oujiang estuary were described using Henry's law. The model was validated using measured data for water surface elevations, flow velocity/direction, suspended sediment concentrations, and the proportion of copper sorbed to sediment. The validated model was then applied to predict fluxes of copper. Combined with the measured data, the copper concentration in the Oujiang River discharge was calculated as 13.0 μg/L and copper fluxes were calculated as 52 t in 2010. This copper flux prediction was verified using measured dissolved copper concentrations. Comparisons between the modeled and measured results showed good agreement at most stations, demonstrating that copper flux prediction in the Oujiang estuary was reasonably accurate.


2021 ◽  
Vol 26 (6) ◽  
pp. 715-726
Author(s):  
Francisco Romero-Gavilán ◽  
Andreia Cerqueira ◽  
Eduardo Anitua ◽  
Ricardo Tejero ◽  
Iñaki García-Arnáez ◽  
...  

AbstractCalcium ions are used in the development of biomaterials for the promotion of coagulation, bone regeneration, and implant osseointegration. Upon implantation, the time-dependent release of calcium ions from titanium implant surfaces modifies the physicochemical characteristics at the implant–tissue interface and thus, the biological responses. The aim of this study is to examine how the dynamics of protein adsorption on these surfaces change over time. Titanium discs with and without Ca were incubated with human serum for 2 min, 180 min, and 960 min. The layer of proteins attached to the surface was characterised using nLC-MS/MS. The adsorption kinetics was different between materials, revealing an increased adsorption of proteins associated with coagulation and immune responses prior to Ca release. Implant–blood contact experiments confirmed the strong coagulatory effect for Ca surfaces. We employed primary human alveolar osteoblasts and THP-1 monocytes to study the osteogenic and inflammatory responses. In agreement with the proteomic results, Ca-enriched surfaces showed a significant initial inflammation that disappeared once the calcium was released. The distinct protein adsorption/desorption dynamics found in this work demonstrated to be useful to explain the differential biological responses between the titanium and Ca-ion modified implant surfaces. Graphic abstract


2016 ◽  
Vol 93 (4) ◽  
pp. 387-394 ◽  
Author(s):  
Chau-Minh Phan ◽  
Magdalena Bajgrowicz ◽  
Huayi Gao ◽  
Lakshman N. Subbaraman ◽  
Lyndon W. Jones
Keyword(s):  

2011 ◽  
Vol 133 (3) ◽  
Author(s):  
Damena D. Agonafer ◽  
J. Yeom ◽  
M. A. Shannon

Microposts are utilized to enhance heat transfer, adsorption/desorption, and surface chemical reactions. In a previous study [Yeom et al., J. Micromech. Microeng., 19, p. 065025 (2009)], based in part on an experimental study, an analytical expression was developed to predict the pressure drop across a microchannel filled with arrays of posts with the goal of fabricating more efficient micro-total analysis systems (µTAS) devices for a given pumping power. In particular, a key figure of merit for the design of micropost-filled reactors, based on the flow resistance models was reported thus providing engineers with a design rule to develop efficient µTAS devices. The study did not include the effects of the walls bounding the microposts. In this paper, a three-dimensional computational fluid dynamics model is used to include the effects of three-dimensionality brought about by the walls of the µTAS devices that bound the microposted structures. In addition, posts of smaller size that could not be fabricated for the experiments were also included. It is found that the two- and three-dimensional effects depend on values of the aspect ratio and the blockage ratios. The Reynolds number considered in the experiment that ranged from 1 to 10 was extended to 300 to help determine the range of Re for which the FOM model is applicable.


2018 ◽  
Vol 32 (10) ◽  
pp. 1392-1405 ◽  
Author(s):  
Ali Deniz Dalgic ◽  
Ammar Z. Alshemary ◽  
Ayşen Tezcaner ◽  
Dilek Keskin ◽  
Zafer Evis

In this study, novel graphene oxide–incorporated silicate-doped nano-hydroxyapatite composites were prepared and their potential use for bone tissue engineering was investigated by developing an electrospun poly(ε-caprolactone) scaffold. Nanocomposite groups were synthesized to have two different ratios of graphene oxide (2 and 4 wt%) to evaluate the effect of graphene oxide incorporation and groups with different silicate-doped nano-hydroxyapatite content was prepared to investigate optimum concentrations of both silicate-doped nano-hydroxyapatite and graphene oxide. Three-dimensional poly(ε-caprolactone) scaffolds were prepared by wet electrospinning and reinforced with silicate-doped nano-hydroxyapatite/graphene oxide nanocomposite groups to improve bone regeneration potency. Microstructural and chemical characteristics of the scaffolds were investigated by X-ray diffraction, Fourier transform infrared spectroscope and scanning electron microscopy techniques. Protein adsorption and desorption on material surfaces were studied using fetal bovine serum. Presence of graphene oxide in the scaffold, dramatically increased the protein adsorption with decreased desorption. In vitro biocompatibility studies were conducted using human osteosarcoma cell line (Saos-2). Electrospun scaffold group that was prepared with effective concentrations of silicate-doped nano-hydroxyapatite and graphene oxide particles (poly(ε-caprolactone) – 10% silicate-doped nano-hydroxyapatite – 4% graphene oxide) showed improved adhesion, spreading, proliferation and alkaline phosphatase activity compared to other scaffold groups.


2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Victor Rodriguez-Lopez ◽  
Carlos Dorronsoro ◽  
Johannes Burge

Abstract Interocular differences in image blur can cause processing speed differences that lead to dramatic misperceptions of the distance and three-dimensional direction of moving objects. This recently discovered illusion—the reverse Pulfrich effect—is caused by optical conditions induced by monovision, a common correction for presbyopia. Fortunately, anti-Pulfrich monovision corrections, which darken the blurring lens, can eliminate the illusion for many viewing conditions. However, the reverse Pulfrich effect and the efficacy of anti-Pulfrich corrections have been demonstrated only with trial lenses. This situation should be addressed, for clinical and scientific reasons. First, it is important to replicate these effects with contact lenses, the most common method for delivering monovision. Second, trial lenses of different powers, unlike contacts, can cause large magnification differences between the eyes. To confidently attribute the reverse Pulfrich effect to interocular optical blur differences, and to ensure that previously reported effect sizes are reliable, one must control for magnification. Here, in a within-observer study with five separate experiments, we demonstrate that (1) contact lenses and trial lenses induce indistinguishable reverse Pulfrich effects, (2) anti-Pulfrich corrections are equally effective when induced by contact and trial lenses, and (3) magnification differences do not cause or impact the Pulfrich effect.


2007 ◽  
Vol 30 (3) ◽  
pp. 183-188 ◽  
Author(s):  
Lívia Santos ◽  
Diana Rodrigues ◽  
Madalena Lira ◽  
M. Elisabete C.D. Real Oliveira ◽  
Rosário Oliveira ◽  
...  

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