scholarly journals Aerobic Isolates from Gestational and Non-Gestational Lactating Bitches (Canis lupus familiaris)

Animals ◽  
2021 ◽  
Vol 11 (11) ◽  
pp. 3259
Author(s):  
Iosif Vasiu ◽  
Gabriele Meroni ◽  
Roman Dąbrowski ◽  
Piera Anna Martino ◽  
Asta Tvarijonaviciute ◽  
...  
Keyword(s):  
Mammary Gland ◽  
Canis Lupus ◽  
Bacterial Colonization ◽  
Bacterial Flora ◽  
Subclinical Mastitis ◽  
Clinical Mastitis ◽  
Canis Lupus Familiaris ◽  
Bacterial Isolation ◽  
Milk Samples ◽  
Bacteriological Profile

Mastitis is a complex and well-defined mammary gland pathology, and an emergency in bitches. In dogs, its prevalence is about 1% of all reported diseases and about 5.3% of all reproductive pathologies. Lactating bitches are naturally prone to developing mastitis since puppies can easily overstimulate the epidermal layer of nipples during feeding, facilitating bacterial colonization of the glands. This study aimed to describe the aerobic bacterial flora isolated from milk samples derived from a cohort of patients (n = 87) diagnosed with clinical mastitis (n = 29), subclinical mastitis (n = 17) and healthy mammary glands (n = 46). All of the patients underwent a gynecology consultation to diagnose mammary gland afflictions; physical examination results were coupled with traditional hematological findings. The milk samples were plated on specific microbiological media for bacterial isolation. Among the 162 milk samples analyzed, 93.2% (151/162) had a positive microbiological result, while 6.8% (11/162) were sterile. The bacteriological profile of the milk samples showed 47 different species. The most common bacterial families detected in healthy bitches and bitches with subclinical and clinical mastitis were the Staphylococcaceae, Enterobacteriaceae and Enterococcaceae families. The results indicated that half of the isolated bacteria are novel findings in dogs and that some of them are normal components of human milk.

Coagulase-negative staphylococci (CNS) as an aetiological factor of mastitis in cows

2013 ◽  
Vol 16 (3) ◽  
pp. 487-492 ◽  
Author(s):  
M. Bochniarz ◽  
W. Wawron ◽  
M. Szczubiał
Keyword(s):  
Mammary Gland ◽  
Subclinical Mastitis ◽  
Clinical Mastitis ◽  
Aetiological Factor ◽  
Coagulase Negative Staphylococcus ◽  
Coagulase Negative Staphylococci ◽  
Milk Samples ◽  
Clinical Forms

AbstractThe aim of the present study was to determine the proportions of individual coagulase-negative Staphylococcus species in clinical and subclinical mastitis. The material consisted of 100 CNS isolates obtained from 223 milk samples collected from cows with clinical and subclinical mastitis. Coagulase- negative staphylococci constituted 44.8% of all isolated microorganisms. CNS were isolated from the mammary gland secretions of 86 cows from farms in the Lublin region (Poland). Clinical mastitis was found in 20 whereas subclinical mastitis in 66 study cows (23.3% and 76.7%, respectively). The symptoms of clinical mastitis were mild. The clinical forms of mastitis concerned mainly the first or second lactation.Subclinical mastitis was most commonly observed during the second lactation. Four CNS species (S. xylosus, S. chromogenes, S. haemolyticus and S. sciuri ) were isolated from clinical and subclinical mastitis. S. xylosus was the commonest CNS species isolated from cows with clinical mastitis whereas S. chromogenes was the most prevalent one in subclinical mastitis cases. The three CNS species (S. warneri, S. hominis and S. saprophyticus) caused only subclinical mastitis.

scholarly journals Mycoplasmal mastitis in dairy cows in the Moghan region of Ardabil State, Iran : short communication

2006 ◽  
Vol 77 (4) ◽  
Author(s):  
C. Ghazaei
Keyword(s):  
Dairy Cows ◽  
Short Communication ◽  
Bovine Mastitis ◽  
Subclinical Mastitis ◽  
Clinical Mastitis ◽  
Mycoplasma Bovis ◽  
Bulk Tank Milk ◽  
Milk Samples ◽  
The World ◽  
Bulk Tank

Mycoplasmas are an important and economically significant cause of mastitis in dairy cows in various parts of the world. The organisms are highly contagious, with the main reservoir of infection originating from cows with subclinical mastitis. In 1998 the 1st cases of bovine mastitis due to Mycoplasma bovis were diagnosed in Ardabil State, Iran. An investigation was carried out with the aim of establishing the extent of mycoplasma infections in dairy cows in Ardabil State. Milk samples obtained from 80 cows with clinical mastitis were cultured in the laboratory for the presence of mycoplasmas. Similarly, 48 bulk-tank milk samples were examined for the presence of mycoplasmas. A modified Hayflick broth was used to isolate the mycoplasmas and an immunoperoxidase test used for the species identification of the isolates. Mycoplasma bovis was isolated from 39 (48.75 %) of the clinical mastitis samples and from 48 of the bulk-tank milk samples tested. This indicated that mycoplasma udder infections were more prevalent in dairy cows in Ardabil State than previously thought.

scholarly journals The presence of pathogens in milk of ewes in relation to the somatic cell count and subpopulations of leukocytes

2021 ◽  
Author(s):  
Tvarožková ◽  
Vašíček ◽  
Uhrinčať ◽  
Mačuhová ◽  
Hleba ◽  
...  
Keyword(s):  
Somatic Cell ◽  
Cell Count ◽  
Somatic Cell Count ◽  
Dairy Farm ◽  
Subclinical Mastitis ◽  
Clinical Mastitis ◽  
Polymorphonuclear Cells ◽  
Coagulase Negative Staphylococci ◽  
Major Health Problem ◽  
Milk Samples

Mastitis is a major health problem of the udder in dairy sheep breeds. For diagnosis of subclinical mastitis, somatic cell count (SCC) is commonly used. The presence of pathogens in the udder causes the increase of leukocytes and thus SCC in milk. Therefore, the aim of this study was to evaluate the presence of pathogens in the milk of ewes and the possible relationship with SCC. The changes of leukocytes subpopulation in milk samples with high SCC were evaluated as well. The experiment was carried out on a dairy farm with the Lacaune breed. This study was conducted on 45 ewes (98 milk samples) without signs of clinical mastitis. Based on somatic cell count, samples were divided to five SCC groups: SCC1 &lt; 200 000 cells/ml (45 milk samples); 200 000 ≤ SCC2 &lt; 400 000 cells/ml (10 milk samples); 400 000 ≤ SCC3 &lt; 600 000 cells/ml (six milk samples); 600 000 ≤ SCC4 &lt; 1 000 000 cells/ml (six milk samples); SCC5 ≥ 1 000 000 cells/ml (31 milk samples). No pathogens were observed in the majority of milk samples (60.20%). Coagulase-negative staphylococci (CNS) were the most commonly isolated pathogens from the milk of ewes (86.11%). Staphylococcus epidermidis had the highest incidence from CNS (35.48%). In the SCC5 group, up to 79.31% of bacteriological samples were positive. The percentage of leukocytes significantly increased (P &lt; 0.001) in the samples with higher SCC (≥ 200 × 10<sup>3</sup> cells/ml) in comparison to the group SCC1. Also, the percentage of polymorphonuclear cells (PMNs) was significantly higher with increasing SCC (P &lt; 0.001). In conclusion, the presented results showed that the high SCC was caused by the presence of the pathogen in milk. Thus SCC &lt; 200 000 cells/ml and leukocyte subpopulation, especially PMNs, could be considered as important tools in udder health programs applied in dairy ewes.

scholarly journals Prevalence of coagulase-negative staphylococci in bovine mastitis in Zimbabwe

2002 ◽  
Vol 73 (2) ◽  
Author(s):  
T. Kudinha ◽  
C. Simango
Keyword(s):  
Somatic Cell ◽  
Enteric Bacteria ◽  
Subclinical Mastitis ◽  
Clinical Mastitis ◽  
Bacillus Species ◽  
Small Scale ◽  
Coagulase Negative Staphylococci ◽  
Somatic Cell Counts ◽  
Milk Samples ◽  
Cell Counts

This study was carried out to determine the prevalence of coagulase-negative staphylococci in clinical and subclinical mastitis in commercial and small-scale farms in Zimbabwe. Thirty five quarter milk samples from clinical mastitis cases and 371 quarter milk samples from cows with subclinical mastitis were cultured for bacterial pathogens. The most frequent pathogens isolated in clinical mastitis were the enteric bacteria (31.4 %), followed by coagulase negative staphylococci (22.9 %) and then Staphylococcus aureus (17.1 %), whereas in subclinical mastitis S. aureus (34.2 %) and coagulase-negative staphylococci were (33.2 %) the most common. Bacillus species were only isolated in milk samples from subclinical mastitis. Coagulase-negative staphylococci were observed in mixed infections with other bacteria in only 2.2 % of the 406 milk samples from clinical and subclinical mastitis where they were isolated together with Bacillus species in 6 of the 9 mixed infection cases. About 95 % of the milk samples from which 131 coagulase-negative staphylococci were isolated had correspondingly high somatic cell counts. The coagulase-negative staphylococci isolated most frequently were S. chromogenes (7.9 %), S. epidermidis (7.4 %) and S. hominis (5.9 %). They were all associated with high somatic cell counts. All the coagulase-negative staphylococci isolates were susceptible to cloxacillin and erythromycin, and more than 90 %of the isolates were susceptible to neomycin, penicillin and streptomycin. The highest resistance was to tetracycline (17.6 %), followed by lincomycin (13.7 %). About 8 % of the isolates were resistant to both penicillin and streptomycin.

scholarly journals Prevalence of microorganisms associated with udder infections in dairy goats on small-scale farms in Kenya : short communication

2001 ◽  
Vol 72 (2) ◽  
Author(s):  
E.N. Ndegwa ◽  
C.M. Mulei ◽  
S.J.M. Munyua
Keyword(s):  
Subclinical Mastitis ◽  
Clinical Mastitis ◽  
Dairy Goat ◽  
Small Scale ◽  
Dairy Goats ◽  
Coagulase Negative Staphylococci ◽  
Milk Samples ◽  
Clinically Normal ◽  
Caprine Milk ◽  
Staphylococcus Spp

Six hundred and thirty clinically-normal milk samples from dairy goat flocks comprising a mixed population of German Alpine, Toggenburg, Saanen and Galla crosses were exam-ined over a 3-month period to determine the prevalence of bacterial organisms. Bacteria were isolated in 28.7 % of the milk samples (181/630) either singly (92.8 %) or in combination (7.2 %). The most prevalent bacterial organisms were Staphylococcus spp. (60.3 %), followed by Micrococcus spp. (17.7 %), Acinetobacter spp. (5 %), Actinomyces spp. (5%) and Streptococcus spp. (1.1 %). The Staphylococcus spp. were mainly coagulase negative (64.3 %). Coagulase-- negative staphylococci and coagulase-positive staphylococci accounted for 37.5 % and 22.7 % respectively of the total bacteria isolated. The isolation of bacteria, some of which are important in clinical and subclinical mastitis, in apparently normal caprine milk, indicates that particular attention should be given to the management of these dairy goat flocks in order to avoid the development of cases of clinical mastitis.

scholarly journals Metagenomic deep sequencing reveals association of microbiome signature with functional biases in bovine mastitis

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
M. Nazmul Hoque ◽  
Arif Istiaq ◽  
Rebecca A. Clement ◽  
Munawar Sultana ◽  
Keith A. Crandall ◽  
...  
Keyword(s):  
Bacterial Colonization ◽  
Bovine Mastitis ◽  
Heavy Metal Resistance ◽  
Clinical Mastitis ◽  
Metal Resistance ◽  
Bacterial Strains ◽  
Viral Genomes ◽  
Milk Samples ◽  
Microbiome Diversity ◽  
Metagenome Sequencing

Abstract Milk microbiomes significantly influence the pathophysiology of bovine mastitis. To assess the association between microbiome diversity and bovine mastitis, we compared the microbiome of clinical mastitis (CM, n = 14) and healthy (H, n = 7) milk samples through deep whole metagenome sequencing (WMS). A total of 483.38 million reads generated from both metagenomes were analyzed through PathoScope (PS) and MG-RAST (MR), and mapped to 380 bacterial, 56 archaeal, and 39 viral genomes. We observed distinct shifts and differences in abundance between the microbiome of CM and H milk in phyla Proteobacteria, Bacteroidetes, Firmicutes and Actinobacteria with an inclusion of 68.04% previously unreported and/or opportunistic strains in CM milk. PS identified 363 and 146 bacterial strains in CM and H milk samples respectively, and MR detected 356 and 251 bacterial genera respectively. Of the identified taxa, 29.51% of strains and 63.80% of genera were shared between both metagenomes. Additionally, 14 archaeal and 14 viral genera were found to be solely associated with CM. Functional annotation of metagenomic sequences identified several metabolic pathways related to bacterial colonization, proliferation, chemotaxis and invasion, immune-diseases, oxidative stress, regulation and cell signaling, phage and prophases, antibiotic and heavy metal resistance that might be associated with CM. Our WMS study provides conclusive data on milk microbiome diversity associated with bovine CM and its role in udder health.

scholarly journals Prevalence of clinical and subclinical mastitis and quality of milk on smallholder dairy farms in Tanzania

2009 ◽  
Vol 80 (3) ◽  
Author(s):  
R.H. Mdegela ◽  
R. Ryoba ◽  
E.D. Karimuribo ◽  
E.J. Phiri ◽  
T. Loken ◽  
...  
Keyword(s):  
Antimicrobial Agents ◽  
Dairy Farms ◽  
Subclinical Mastitis ◽  
Clinical Mastitis ◽  
Milk Quality ◽  
Herd Level ◽  
Milk Samples ◽  
Antimicrobial Residues ◽  
Smallholder Dairy ◽  
Smallholder Dairy Farms

A cross sectional study was conducted during October and November 2006 on 69 smallholder dairy farms with lactating cows in Mvomero and Njombe districts Tanzania, to determine the prevalence of mastitis and to assess the milk quality on the study farms. Clinical mastitis was investigated using clinical changes of udder and milk at animal level. Cow-side California Mastitis Test (CMT) and microbiological cultures were used to assess subclinical mastitis at quarter level. Milk quality was determined on bulk milk samples at herd level using alcohol and acidity tests, butter fat content, total solids, ash content as well as Delvotest® for antimicrobial residues. Overall prevalence of clinical mastitis at herd level in both districts was 21.7 % (n = 69). Based on CMT, prevalence of subclinical mastitis at animal level was 51.6 % (n = 91). Prevalence of bacterial isolates at animal level was 35.2 % (n = 91) while for fungal it was 16.7 % (n = 90). Based on CMT results, prevalence of subclinical mastitis at quarter level was 30 % (n = 353), while for bacteria and fungi it was 16 % and 6 % respectively. Contamination of milk with antimicrobial residues was 4.5 % (n =67). The milk quality parameters for most of the milk samples were within acceptable levels. Findings in this study have demonstrated high prevalence of subclinical mastitis that may contribute to low productivity of dairy cattle in both districts. About 20 % of CMT subclinical cases had no involvement of microbial pathogens that suggested the need for minimal interventions with antimicrobial agents. These findings call for use of udder disinfectants and improved milking hygiene as intervention strategies to control mastitis on the smallholder dairy farms in Tanzania.

scholarly journals The Impact of Mastitis on Reproductive Parameters of Dairy Cows

2019 ◽  
Vol 63 (3) ◽  
pp. 72-78
Author(s):  
F. Zigo ◽  
A. Adamová ◽  
M. Vasiľ ◽  
J. Elečko ◽  
S. Ondrašovičová ◽  
...  
Keyword(s):  
Dairy Cows ◽  
Subclinical Mastitis ◽  
Clinical Mastitis ◽  
Reproductive Parameters ◽  
Coagulase Negative Staphylococci ◽  
Milk Samples ◽  
California Mastitis Test ◽  
Service Period ◽  
High Incidence ◽  
The Impact

Abstract The aim of this study was to evaluate the occurrence of mastitis and its impact on the reproductive parameters in a herd of 180 dairy cows. Based on the herd records 127 cows of Slovak spotted cattle and their crosses with red Holstein were selected for study between 1—2 months after calving. The examination of the health status of the mammary glands consisted of: the clinical examination of the udder, the California mastitis test (CMT) supplemented by the collection of mixed milk samples, and the laboratory examination of bacterial pathogens causing the mastitis. In addition to the mam­mary investigation, reproduction indicators such as the length of the insemination interval, the service period, the intercalving period and the insemination index were also analyzed. The results of this study indicated: a high incidence of mastitis (41.6 %), especially latent (21.2 %), subclinical (15.7 %) and clinical (4.7 %) forms were most common in the herd. The most frequently isolated bacteria from the infected milk samples were: coagulase negative staphylococci (54.1 %), S. aureus (16.9 %), Streptococcus spp. (15.0 %), A. viridans (7.5 %) and Ent. faecalis (6.4 %). According to the available literature, the optimum values of the intercalving period were 365—400 days, the insemination interval 55—80 days, the insemination index 1.2—2 and the service period 60—110 days. In comparison, our results showed increased, unsatisfactory reproductive values in the group of dairy cows with clinical mastitis. While in healthy cows as well as in groups of cows with latent and subclinical mastitis, all of the reproductive indicators were within the optimal levels.

scholarly journals Sensitivity and Resistance of the Microbiota of Reproductive Organs and Mammary Gland of Cows to Anti-microbial Agents in Cases of Inflammation

2020 ◽  
Vol 14 ◽  
Keyword(s):  
Mammary Gland ◽  
Inflammatory Diseases ◽  
Culture Method ◽  
Subclinical Mastitis ◽  
Reproductive Organs ◽  
Clinical Mastitis ◽  
Candida Spp ◽  
E Coli ◽  
Opportunistic Bacteria ◽  
Bacillus Spp

This paper contains the results of complex microbiological studies (culture method, real-time PCR) of biological material from cows affected by inflammatory diseases of reproductive organs and mammary gland. Milk microbiota with underlying subclinical mastitis was represented by pathogenic (S. aureus, enteropathogenic E. coli), opportunistic bacteria (Staphylococcus spp., E. coli, E. faecium, Streptococcus spp., S. agalactiae, P. aeruginosa) and yeast-like fungi of Candida spp. in association. Combined infectious and inflammatory diseases of genital tract and mammary gland in cows resulted in the same types of microorganisms in microflora composition, which confirms information obtained by other researchers on associated microbiota during inflammatory processes. S. aureus, P. aeruginosa, E. coli, E. faecium, E. faecalis, S. epidermidis, S. saprophyticus, Bacillus spp., and yeast-like fungi of Candida spp. were obtained from milk samples from cows with acute clinical mastitis and in vaginal washes from cows with acute postpartum endometritis. In the course of defining the sensitivity to antibiotics and identifying genes of antimicrobial resistance, multidrug resistance was established in most part of obtained strains, which should be taken into account when planning treatment measures.

scholarly journals Yeasts as a causative agent of bovine mastitis in Greece

2018 ◽  
Vol 54 (2) ◽  
pp. 105 ◽  
Author(s):  
E. BOURTZI-HATZOPOULOU (Ε. ΜΠΟΥΡΤΖΗ-ΧΑΤΖΟΠΟΥΛΟΥ) ◽  
A. ZDRAGAS (Α. ΖΔΡΑΓΚΑΣ) ◽  
E. PETRIDOU (Ε. ΠΕΤΡΙΔΟΥ) ◽  
G. FILIOUSIS (Γ. ΦΙΛΙΟΥΣΗΣ)
Keyword(s):  
Mammary Gland ◽  
Dairy Cows ◽  
Bovine Mastitis ◽  
Economic Loss ◽  
Geotrichum Candidum ◽  
Clinical Mastitis ◽  
Antibacterial Treatment ◽  
Northern Greece ◽  
Milk Samples ◽  
Mastitic Milk

The aim of this study was to isolate fungi from mastitic milk of dairy cows and to identify fungal microorganisms involved in bovine mastitis. A total of 608 milk samples from clinical mastitis quarters from 580 animals in Northern Greece were collected, during the years 1997-2001. Antibacterial treatment was administrated to 50% of the sampled animals. Forty two (42) fungi were isolated and identified using mycological media and methods. From the 42 fungi isolates, 38 were yeasts and 4 moulds. The yeasts isolated were classified into the genera Candida, Geotrichum, Rhodotorula. From the thirty four (34) Candida species, 14 were identified as G tropicalis and 6 as G krusei. Furthermore, 4 isolates were classified as G pseudotropicalis, 4 as G albicans, 3 as G parapsilosis and 3 as G rugosa. Geotrichum candidum and Rhodotorula spp. were represented with 2 isolates. The isolated moulds were classified into the genus Aspergillus. Fungi were isolated in pure culture from 38 milk samples and in mixed culturewith bacteria from 4. In 510 samples only bacteria were cultured, while in 56 samples no growth of microorganism was observed. The results of the present study indicate that a level of 6.9% of mycotic mastitis is significant and yeasts are apparently implicated in mammary gland pathology causing economic loss.

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