Abstract
In the context of global warming, the bioelectrochemical method (microbial fuel cell MFC) was proposed for CH4 control from CWs. The main focus is to further explore the effect of plant roots location at the electrode, plant species on CH4 emissions, bioelectricity generation and the mechanism underlying competition between electrogenesis and methanogenesis at the anode. The results showed that the operation of MFC effectively reduced the CH4 emissions and promoted COD removal rates. CH4 emission was significantly higher in open circuit (6.2 mg m-2 h-1) than in closed circuit reactors (3.1 mg m-2 h-1). Plant roots at the cathode had the highest electricity generation and the lowest CH4 emissions. The highest power generation (0.49 V, 0.33 w m-3) and the lowest CH4 emissions (2.3 mg m-2 h-1) were observed in the reactors where Typha Orientalis was planted with plant roots at the cathode. The role of plants in strengthening electron acceptor was greater than that of plant rhizodeposits in strengthening electron donors. q-PCR and correlation analysis indicated that the mcrA genes and CH4 emissions were positively correlated (r=0.98, p<0.01), while no significant relationship between CH4 emissions and pmoA genes was observed. More nanowires, which are conductive to electron transfer, were found when plant roots were in cathode by scanning electron microscope (SEM). Illumina sequencing revealed that more abundant exoelectrogens and denitrifying bacteria (Geobacter, Desulfobulbu, Nitrospira and Anaerolinea) were observed when plant roots located in cathodes. Strictly acetotrophic archae (Methanosaetaceae) were likely main electron donor competitors with exoelectrogens. In addition, plant species played a more important role in CH4 emissions and electricity generation than the plant roots location at the electrode. Therefore, it is necessary to strengthen plant configuration to reduce CH4 emissions, so as to promote sustainable development of wastewater treatment.