X-linked intellectual disability mutations alter the conformational state of a histone demethylase's sensing of chromatin

The H3K4me3 chromatin modification, a hallmark of promoters of actively transcribed genes, is dynamically removed by the KDM5 family of histone demethylases. The KDM5 demethylases have a number of accessory domains, two of which, ARID and PHD1, lie within the catalytic domain. KDM5C, which has a unique role in neural development, harbors a number of mutations adjacent to its accessory domains that cause X-linked intellectual disability (XLID). The roles of these accessory domains remain unknown, limiting an understanding of how XLID mutations affect KDM5C activity. We find that while the ARID and PHD1 domains are required for efficient nucleosome demethylation, the PHD1 domain alone has an inhibitory role in KDM5C catalysis. We further find that binding of the H3 tail to PHD1 is coupled to the recognition of linker DNA by KDM5C. Our data suggests a model in which the PHD1 domain regulates DNA recognition by the ARID domain based on available substrate cues. In this model, recognition of distinct chromatin features is coupled to a conformational rearrangement of the ARID and PHD1 domains, which in turn modulates the positioning of the catalytic domain for efficient nucleosome demethylation. Importantly, we find that XLID mutations adjacent to the ARID and PHD1 domains alter the conformational state of these domains to enhance DNA binding. This results in the loss of specificity in chromatin recognition by KDM5C and renders catalytic activity sensitive to inhibition by linker DNA. Our findings suggest a unifying model by which XLID mutations alter chromatin recognition and enable euchromatin-specific dysregulation of demethylation by KDM5C.

Improved Mask R-CNN for Rural Building Roof Type Recognition from UAV High-Resolution Images: A Case Study in Hunan Province, China

Accurate roof information of buildings can be obtained from UAV high-resolution images. The large-scale accurate recognition of roof types (such as gabled, flat, hipped, complex and mono-pitched roofs) of rural buildings is crucial for rural planning and construction. At present, most UAV high-resolution optical images only have red, green and blue (RGB) band information, which aggravates the problems of inter-class similarity and intra-class variability of image features. Furthermore, the different roof types of rural buildings are complex, spatially scattered, and easily covered by vegetation, which in turn leads to the low accuracy of roof type identification by existing methods. In response to the above problems, this paper proposes a method for identifying roof types of complex rural buildings based on visible high-resolution remote sensing images from UAVs. First, the fusion of deep learning networks with different visual features is investigated to analyze the effect of the different feature combinations of the visible difference vegetation index (VDVI) and Sobel edge detection features and UAV visible images on model recognition of rural building roof types. Secondly, an improved Mask R-CNN model is proposed to learn more complex features of different types of images of building roofs by using the ResNet152 feature extraction network with migration learning. After we obtained roof type recognition results in two test areas, we evaluated the accuracy of the results using the confusion matrix and obtained the following conclusions: (1) the model with RGB images incorporating Sobel edge detection features has the highest accuracy and enables the model to recognize more and more accurately the roof types of different morphological rural buildings, and the model recognition accuracy (Kappa coefficient (KC)) compared to that of RGB images is on average improved by 0.115; (2) compared with the original Mask R-CNN, U-Net, DeeplabV3 and PSPNet deep learning models, the improved Mask R-CNN model has the highest accuracy in recognizing the roof types of rural buildings, with F1-score, KC and OA averaging 0.777, 0.821 and 0.905, respectively. The method can obtain clear and accurate profiles and types of rural building roofs, and can be extended for green roof suitability evaluation, rooftop solar potential assessment, and other building roof surveys, management and planning.

Detection Based on Crack Key Point and Deep Convolutional Neural Network

Based on the features of cracks, this research proposes the concept of a crack key point as a method for crack characterization and establishes a model of image crack detection based on the reference anchor points method, named KP-CraNet. Based on ResNet, the last three feature layers are repurposed for the specific task of crack key point feature extraction, named a feature filtration network. The accuracy of the model recognition is controllable and can meet both the pixel-level requirements and the efficiency needs of engineering. In order to verify the rationality and applicability of the image crack detection model in this study, we propose a distribution map of distance. The results for factors of a classical evaluation such as accuracy, recall rate, F1 score, and the distribution map of distance show that the method established in this research can improve crack detection quality and has a strong generalization ability. Our model provides a new method of crack detection based on computer vision technology.

Aircraft Type Recognition in Remote Sensing Images: Bilinear Discriminative Extreme Learning Machine Framework

Small inter-class and massive intra-class changes are important challenges in aircraft model recognition in the field of remote sensing. Although the aircraft model recognition algorithm based on the convolutional neural network (CNN) has excellent recognition performance, it is limited by sample sets and computing resources. To solve the above problems, we propose the bilinear discriminative extreme learning machine (ELM) network (BD-ELMNet), which integrates the advantages of the CNN, autoencoder (AE), and ELM. Specifically, the BD-ELMNet first executes the convolution and pooling operations to form a convolutional ELM (ELMConvNet) to extract shallow features. Furthermore, the manifold regularized ELM-AE (MRELM-AE), which can simultaneously consider the geometrical structure and discriminative information of aircraft data, is developed to extract discriminative features. The bilinear pooling model uses the feature association information for feature fusion to enhance the substantial distinction of features. Compared with the backpropagation (BP) optimization method, BD-ELMNet adopts a layer-by-layer training method without repeated adjustments to effectively learn discriminant features. Experiments involving the application of several methods, including the proposed method, to the MTARSI benchmark demonstrate that the proposed aircraft type recognition method outperforms the state-of-the-art methods.

Hierarchical Scheme for Vehicle Make and Model Recognition

A vehicle make and model recognition (VMMR) system is a common requirement in the field of intelligent transportation systems (ITS). However, it is a challenging task because of the subtle differences between vehicle categories. In this paper, we propose a hierarchical scheme for VMMR. Specifically, the scheme consists of (1) a feature extraction framework called weighted mask hierarchical bilinear pooling (WMHBP) based on hierarchical bilinear pooling (HBP) which weakens the influence of invalid background regions by generating a weighted mask while extracting features from discriminative regions to form a more robust feature descriptor; (2) a hierarchical loss function that can learn the appearance differences between vehicle brands, and enhance vehicle recognition accuracy; (3) collection of vehicle images from the Internet and classification of images with hierarchical labels to augment data for solving the problem of insufficient data and low picture resolution and improving the model’s generalization ability and robustness. We evaluate the proposed framework for accuracy and real-time performance and the experiment results indicate a recognition accuracy of 95.1% and an FPS (frames per second) of 107 for the framework for the Stanford Cars public dataset, which demonstrates the superiority of the method and its availability for ITS.

Automatic reservoir model identification method based on convolutional neural network

Well test analysis is a crucial technique to monitor reservoir performance, which is based on the theory of seepage mechanics, through the study of well test data, to identify reservoir models and estimate reservoir parameters. Reservoir model recognition is the first and essential step of well test analysis. It is usually judged by professionals' experience, which results in low efficiency and accuracy. This paper is devoted to applying convolutional neural network (CNN) to well test analysis and proposes a new intelligent reservoir model identification method. Eight reservoir models studied in this paper include homogenous reservoirs with different outer boundaries such as infinite acting boundary, circular, single, angular, channel, U-shaped and rectangular sealing fault boundaries and a radial composite reservoir with infinite acting boundary. Well testing data used in this paper, including actual field data and theoretical data generated by analytical solutions. To improve the classification accuracy of actual field data, noise processing was carried out on the data before training. The CNN that is most suitable for model recognition has been obtained through trial-and-error procedures. The availability of proposed CNN is proved with actual field cases of Daqing oil field, China. The method realizes the automatic identification of reservoir model with the total classification accuracy (TCA) of test data set of 98.68% and 95.18% for original data and noisy data respectively.