Morphological And Molecular Identification of Double Flowered Stock (Matthiola Incana R. Br) Cultivars With High Fertility

Abstract Garden stock (Matthiola incana R. Br.) is a commercially important horticultural crop owing to its ornamental effect. There are different stock cultivars varied in color and shape, especially flowered phenotype is an essential index evaluating its commercial value, because double flowered cultivars have more brilliant flowers compared to single flowered one. The present work aimed: (1) to make superior cultivars with different colors, high fertility, being capable of early selecting only double flowered seedlings by leaf color and to investigate morphological characteristics and (2) to select RAPD and ISSR primers for the cultivar certification and identification to culture and produce good commercial stock cultivars. Here we obtained new double flowered stock cultivars with different colors including pink, pale pink and white, through outcrossing between “white” cultivar (high fertile but unable to select double flower phenotype) and “pink” cultivar (vice. versa). Among newly obtained stock cultivars, single and double flower seedlings are distinguishable from each other by leaf color, having about 70% of fertility. Moreover RAPD and ISSR markers selected in this study can be applied to identify different stock cultivars in seed production, culture and to establish cultivar certification system.

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Rapid Plant Regeneration and Molecular Assessment of Genetic Stability Using ISSR and RAPD Markers in Commercial Banana Cv. Grand Naine (G-9)

JOURNAL OF ADVANCES IN BIOTECHNOLOGY ◽

10.24297/jbt.v4i3.4890 ◽

2014 ◽

Vol 4 (3) ◽

pp. 393-403

Author(s):

J. S. DUHAN ◽

S. Kajla ◽

D. Choudhary ◽

A. K. Poonia

Keyword(s):

Tissue Culture ◽

Genetic Stability ◽

Random Amplified Polymorphic Dna ◽

Issr Markers ◽

Ms Medium ◽

Inter Simple Sequence Repeats ◽

Rapd Primer ◽

Molecular Assessment ◽

Rapd And Issr ◽

Issr Primers

The investigation was carried out to assess the genetic stability in Â Â tissue culture raised plants of banana cv. G-9 using random amplified polymorphic DNA (RAPD) and inter simple sequence repeats (ISSR) markers.Aims:Â Molecular assessment of genetic stability of tissue culture raised plants of banana cv. G-9 using molecular markers.Material and Results: Apical shoots were established on mediumÂ EM4Â (MS + BAP 4.0 mg L-1) with maximum of 3.8 buds/explant in 2.6 days. The maximum bud multiplicationÂ withÂ 16.5Â±0.06 shootsÂ was observed on mediumÂ Ma3Â (MS medium+ 5.0 mg L-1Â BAP + 0.25 mg L-1Â NAA ofÂ + 30 mg L-1Â AdSO4).Â The maximum rooting response (100%) wasÂ observed on 1/2 MS medium supplemented with 2.0 mg L-1Â NAA inÂ 12.2 days. After acclimatization the hardened plants were examined for genetic stability using RAPD and ISSR primers. Total forty six (twenty six RAPD and twenty ISSR) markers were used. RAPD primers produced 87 distinct and scorable bands, with an average of 3.34 bands per primer and the amplification products range was from 100-1200 bps. The number of scorable bands for RAPD primer varied from 2 to 5 with an average of 3.34 bands per primer. ISSR primers produced 71 distinct and scorable bands in the range of 100-1000 bps and the number of scorable bands for each primer varied from 2 to 6 with an average of 3.55 bands per primer.Conclusion: Similar profile with monomorphic bands was observed for all the tissue culture raised plants when compared to mother plant in both types of markers used. The results corroborate the fact that plant tissue culture technology has immense importance for production of true to type of planting material.Â

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Cytogenetic and molecular studies on two faba bean cultivars revealed their difference in their aluminum tolerance

Acta agriculturae Slovenica ◽

10.14720/aas.2020.116.2.1346 ◽

2020 ◽

Vol 116 (2) ◽

pp. 273

Author(s):

Ahmed M. HASSANEIN ◽

Ahmed H. MOHAMED ◽

Heba Ahmed ABD ALLAH ◽

Hoida ZAKI

Keyword(s):

Faba Bean ◽

Chromosomal Abnormalities ◽

Aluminum Tolerance ◽

Issr Markers ◽

Growth Data ◽

Al Tolerance ◽

Al Stress ◽

Molecular Studies ◽

Rapd And Issr ◽

Issr Primers

Two cultivars of faba bean (Vicia faba ‘Giza 843’ and ‘Nobaria 3’) that differ in aluminum (Al) tolerance were used to study cytogenetic and genomic alterations under the influence of Al Cl3 (5, 15, and 25 mmol AlCl3) for different periods (6, 12 and 24 h). Under Al treatments, mitotic index in both cultivars decreased and total chromosomal abnormalities increased. The frequencies of micronuclei and chromosomal abnormalities (C-anaphase, metaphase-star chromosomes, breaks, sticky and disturbed chromosomes during metaphase or anaphase) in ‘Giza 843’ were lower than in ‘Nabaria 3’. Increase of the registered cytogenetic events under the influence of Al stress led to increase the detected polymorphism using RAPD and ISSR markers. Application of RAPD primers gave the same value of polymorphism in both faba bean cultivars under Al stress. Polymorphism average of nine ISSR primers of ’Giza 843’ (65.36 %) was lower than that of ‘Nobaria 3’ (71.59 %). Molecular markers, cytogenetic characteristics and seedling growth data indicate that Al tolerance of ‘Giza 843’ was higher than of ‘Nobaria 3’. This work shows that cytogenetic and ISSR techniques could be used efficiently to distinguish between the ability of two faba bean cultivars to tolerate toxic effects of Al.

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Application of RAPD and ISSR markers to analyse molecular relationships in Grevillea (Proteaceae)

Australian Systematic Botany ◽

10.1071/sb03016 ◽

2004 ◽

Vol 17 (1) ◽

pp. 49 ◽

Cited By ~ 22

Author(s):

M. Pharmawati ◽

G. Yan ◽

I. J. McFarlane

Keyword(s):

Phylogenetic Trees ◽

Genetic Relationships ◽

Initial Point ◽

Morphological Characteristics ◽

Issr Markers ◽

Group Method ◽

Pair Group ◽

Molecular Relationships ◽

Group B ◽

Rapd And Issr

The potential of RAPD and ISSR markers to construct molecular relationships of Grevillea was evaluated with 23 RAPD and 12 ISSR primers. The 16 genotypes representing 12 species and 3 subspecies of Grevillea were sampled from the collection of the Mt Anann Botanic Garden, NSW. RAPD and ISSR assays generated a total of 401 RAPD and 280 ISSR fragments. High frequencies of polymorphisms, 99.39% for RAPD and 99.51% for ISSR, were detected by both markers. Three statistical approaches were employed to construct phylogenetic relationships from combined RAPD and ISSR data. Cluster analysis by the unweighted pair group method (UPGMA) of Jaccard's similarity and Neighbour-Joining analysis of total character difference generated dendograms with similar topology. Parsimony analysis also generated a tree that was in broad agreement with the two dendograms. The phylogenetic trees divided the Grevillea species studied into three groups. Group A consisted of G. buxifolia subsp. buxifolia, G. phylicoides and G. sphacelata. In group B, G. mucronulata was grouped together with G. montana, while G. diffusa, G. humilis, G. linearifolia, G. molyneuxii, G. oldei, G. sericea and G. speciosa formed group C. This molecular result was comparable to groupings suggested by a previous author (Makinson 2000) based on morphological characteristics. However, in contrast to the morphological taxonomy, molecular phylogeny suggests that G. oldei and G. speciosa belong to the same subgroup sensu Makinson (2000), whereas G. linearifolia and G. molyneuxii should not be placed in their originally suggested subgroups sensu Makinson (2000). The present study is the first published report on molecular relationships of Grevillea and can be considered as an initial point for further research on the genetic relationships and evolution of Grevillea.

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Genetic Relationship in Sainfoin (Onobrychis viciifolia) Landraces Cultivated East Anatolia by using RAPD and ISSR Markers

Legume Research - An International Journal ◽

10.18805/lr-614 ◽

2021 ◽

Author(s):

Mehmet Macit Ertuş

Keyword(s):

Genetic Diversity ◽

Diversity Index ◽

Calcareous Soils ◽

Similarity Index ◽

Issr Markers ◽

High Genetic Diversity ◽

Onobrychis Viciifolia ◽

Young Leaves ◽

Rapd And Issr ◽

Issr Primers

Background: Sainfoin (Onobrychis viciifolia) is a forage crop that yields high in arid and calcareous soils and is cultivated in large areas. There aren’t many genetic diversity studies on the varieties of cultured sainfoin. This study was conducted to determine the genetic diversity and the degree of relationship between 23 cultivated landraces and one registered variety. Methods: To take samples from the populations, seeds were sown in the field in 2014. Samples were taken from the young leaves of the plants and preserved at -80oC in same year. RAPD and ISSR primers were used in the study. The bands obtained as a result of PCR were recorded and the data of both methods were also evaluated by combining them. Result: In the study, 5 RAPD and 4 ISSR primers were used and a total of 49 bands were obtained. Of 29 bands obtained using RAPD primers, 20 were found to be polymorphic and of 20 bands obtained using ISSR primers, 15 were found to be polymorphic. It was found that there was a very low correlation between the two methods. Using RAPD and ISSR markers and RAPD + ISSR combination, the similarity index among populations was found to be between 0.25-0.95, 0.5-1.00 and 0.45-0.91, respectively. The Nei’s genetic diversity index was found to be between 0.3365, 0.2656 and 0.3018 with RAPD, ISSR primers and RAPD + ISSR combination, respectively. Based on the dendrograms obtained using RAPD, ISSR primers and RAPD + ISSR combination, the populations under analysis were classified into 3, 3 and 5 groups, respectively. With this study, the closest populations were identified and a significantly high genetic diversity was detected.

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Molecular Characterization and Analysis of the Greek Citrus Germplasm

Notulae Botanicae Horti Agrobotanici Cluj-Napoca ◽

10.15835/nbha4129302 ◽

2013 ◽

Vol 41 (2) ◽

pp. 463 ◽

Cited By ~ 3

Author(s):

Constantinos TRIPOLITSIOTIS ◽

Nikolaos NIKOLOUDAKIS ◽

Athanasios LINOS ◽

Marianna HAGIDIMITRIOU

Keyword(s):

Sweet Orange ◽

Issr Markers ◽

Molecular Techniques ◽

Discrimination Power ◽

Breeding Programs ◽

High Affinity ◽

Citrus Germplasm ◽

Highly Correlated ◽

Rapd And Issr ◽

Issr Primers

Molecular markers were used to evaluate genetic similarity among 36 citrus accessionsc (C. sinensis, C. reticulata, C. limon and cultivars) of the Greek national clonal germplasm repository located in Poros. In total, nine RAPD and five ISSR primers produced 262 loci, of which 236 were polymorphic. Both techniques were proven to be equally analytical with an average discrimination power above 0.9. The RAPD and ISSR markers were highly correlated and clustering among them had high correspondence. Citrus accessions formed separate clusters according to their species, even though sweet orange and mandarin cultivars revealed high affinity, while lemons were more divergent. C. sinensis genotypes had the highest intragenetic affinity suggesting that cultivated oranges have a narrow genetic base. On the contrary, the C. reticulata group was the most variable. In general, Greek varieties exhibited a distinctive genetic background that could be discriminated from foreign established cultivars using the mentioned molecular techniques. This information could facilitate their use in future Citrus spp. breeding programs.

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RAPD and ISSR Methods Used for Fingerprinting of Selected Accessions of Viburnum

Notulae Scientia Biologicae ◽

10.15835/nsb639422 ◽

2014 ◽

Vol 6 (3) ◽

pp. 292-299

Author(s):

Marcelina KRUPA-MAŁKIEWICZ ◽

Miłosz Smolik ◽

Anna BARNIAK ◽

Beata SMOLIK

Keyword(s):

Genetic Variability ◽

Inter Simple Sequence Repeat ◽

Issr Markers ◽

Mantel Test ◽

North West ◽

The North ◽

West Part ◽

Rapd And Issr ◽

Simple Sequence ◽

Issr Primers

Randomly amplified polymorphic DNA (RAPD) and inter simple sequence repeat (ISSR) markers were used to investigate genetic variability within thirteen Viburnum species (Viburnum × hillieri; V. dilatatum; Viburnum × carlcephalum; V. opulus; V. hupehense; Viburnum× bodnantense; Viburnum × burkwoodii; V. sieboldii; Viburnum × globosum ‘Jermyns Globe’; V. alnifolium (lantanoides); V. plicatum ‘Sterile’; V. plicatum f. tomentosum and V. plicatum ‘Watanabe’) of wide geographical distribution, collected in the Dendrological Garden in Przelewice (the north-west part of Poland). Twenty-three RAPD and fourteen ISSR primers generated a total of 690 and 418 reproducible bands, respectively, and 39% (RAPD) and 55.5% (ISSR) of them were polymorphic for the two marker systems, which suggest high genetic variability within Viburnum genus. However, high numbers of genotype-specific bands, i.e. 60.9% (RAPD) and 44.5% (ISSR), were seen in Viburnum. Genetic similarity assessed within Viburnum species with the RAPD and ISSR analyses ranged from 6 to 42% and from 6 to 31%, respectively. Both RAPD and ISSR-based dendrograms clustered in five main groups. The Mantel test between two Nei’s similarity matrices gave correlation coefficient r=0.305*, showing low correlation between RAPD- and ISSR- based matrices. Thus, both marker systems were equally important for the genetic diversity analysis in Viburnum genus.

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Genetic diversity of chickpea genotypes as revealed by ISSR and RAPD markers

Genetika ◽

10.2298/gensr1702415h ◽

2017 ◽

Vol 49 (2) ◽

pp. 415-423

Author(s):

Saida Hasanova ◽

Zeynal Akparov ◽

Alamdar Mammadov ◽

Litfer Amirov ◽

Sevda Babayeva ◽

...

Keyword(s):

Genetic Diversity ◽

Rapd Markers ◽

Diversity Index ◽

Issr Markers ◽

Similarity Coefficient ◽

Parental Lines ◽

Spss Software ◽

Rapd And Issr ◽

Combined Data ◽

Issr Primers

Genetic diversity of 62 chickpea accessions was studied using 8 ISSR and 11 RAPD primers. In the study RAPD primers detected more polymorphism (98%) than the ISSR primers (80%). Genetic diversity index was high (0.73 for ISSR and 0.85 for RAPD) for each of these marker systems. Cluster analysis performed from both separate and combined data of RAPD and ISSR markers using SPSS software package. Jaccard?s similarity coefficient for 62 chickpea genotypes was 0.65. Cluster analyses based on combined data generated a dendrogram that separated genotypes into 11 clusters. Four clusters contained only one genotype showing the genetic uniqueness of these accessions. The studied chickpea collection has been proved to constitute a rich source of biodiversity as revealed by RAPD and ISSR markers. Crossing between distantly related genotypes is expected to yield more vigorous plants constituting much of the different traits contained in the two parental lines.

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Molecular identification and genetic relationships among Alcea (Malvaceae) species by ISSR Markers: A high value medicinal plant

Caryologia ◽

10.36253/caryologia-1330 ◽

2021 ◽

Vol 74 (3) ◽

pp. 65-75

Author(s):

Jinxin Cheng ◽

Dingyu Hu ◽

Yaran Liu ◽

Zetian Zhang ◽

Majid Khayatnezhad

Keyword(s):

Genetic Relationships ◽

Morphological Characteristics ◽

Molecular Data ◽

Issr Markers ◽

Great Capacity ◽

Inter Simple Sequence Repeats ◽

Plant Recognition ◽

The Family ◽

Simple Sequence ◽

Issr Primers

Alcea L. is one of the largest genera of Malvaceae family with nearly 70 species worldwide mainly distributed in SW Asia. According to the latest revision of the family, it is represented by 34 species in the Flora of Iran, among them, 15 species are endemic. It is tough to accurate germplasm/ plant recognition by using morphological characteristics because of its propagation, growing and using. We conducted a molecular data analysis on these plant species due to their importance. We examined 156 plants from 14 species in 16 regions that were selected randomly for this investigation. It has been 119 polymorphic bands (94.33%) were resulted from 128 bands of 10 primers in amplification of genomic DNA. ISSR primers have a great capacity to detect polymorphic loci among Alcea species, as evidenced by the high average PIC and MI values found. The genetic similarity of 14 species was calculated and ranged between 0.635 to 0.990. Inter-Simple sequence repeats (ISSR) markers research revealed that Alcea tarica Pakravan & Ghahreman and Alcea kopetdaghensis lljin had the least similarity, while Alcea semnanica Pakravan and Alcea mazandaranica Pakravan & Ghahreman had the most. The current study attempts to answer three questions: 1) can ISSR markers identify Alcea species? 2) what is the genetic structure of these taxa in Iran? and 3) what is the inter-relationship between these taxa? The current study discovered that ISSR markers can be used to identify species.

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Validation of RAPD and ISSR Markers Used for Sex Determination in Date Palm Grown under Sudan Conditions

Advanced Research in Life Sciences ◽

10.2478/arls-2019-0005 ◽

2019 ◽

Vol 3 (1) ◽

pp. 17-22

Author(s):

Ismail A. Mohammed ◽

Israa A. Mohamed

Keyword(s):

Sex Determination ◽

Date Palm ◽

Early Stage ◽

Pcr Amplification ◽

Issr Markers ◽

Ctab Method ◽

Male Specific ◽

Rapd And Issr ◽

Issr Primers

Abstract Date palm is one of the most important economical crops in the world. Sex determination of date palm in early stage is a prerequisite for breeding and cultivation. The aim of this study is to validate RAPD and ISSR markers for sex identification of date palm genotypes grown under Sudan conditions. DNA was extracted from ten seedlings and five male and female plants using CTAB method. Eight primers, six RAPD and two ISSR primers were examined for their validation in sex determination of date palm genotypes. PCR amplification was performed using these primers. Four RAPD primers OPA02, OPJ-09, RD A02 and RD A21 were amplified male specific band with size of 1000, 1100, 1000 and 1400 pb respectively, while ISSR markers could not. The specific bands were observed clearly among all male genotypes and absent in female samples unknown samples irrespective of genotypes. Our results could be useful for sex determination of date palm sex in seedling stage and would promote date palm cultivation and production.

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