Reinstatement of Indian Ocean Porolithon coarctatum and P. gardineri based on sequencing type specimens, and P. epiphyticum sp. nov. (Corallinales, Rhodophyta), with comments on subfamilies Hydrolithoideae and Metagoniolithoideae

Partial rbcL sequences were obtained from the type specimens of Lithophyllum coarctatum and L. gardineri. Both species were variably assigned to either Hydrolithon or Porolithon during the last two centuries, with L. coarctatum, from the tropical eastern Indian Ocean currently considered a synonym of L. gardineri from the tropical western Indian Ocean. Phylogenetic analyses show that both belong in Porolithon and that they are distinct species despite their morpho-anatomical similarities, including a columnar morphology. Porolithon epiphyticum sp. nov., from the same locality as P. coarctatum, Cocos-Keeling Islands, is the first reported epiphytic species in the genus. Eight of the currently recognized 15 species of Porolithon have had their type specimens sequenced to correctly apply names, including the generitype specimen. DNA sequencing provides independent confirmation that the morpho-anatomical character of the presence of horizontal fields of trichocytes without intervening vegetative cells is diagnostic for Porolithon. The generitypes of the four genera classified in the subfamily Metagoniolithoideae, Dawsoniolithon, Floiophycus, Harveylithon, and Metagoniolithon as well as Hydrolithon in the subfamily Hydrolithoideae, have not been sequenced. The taxonomic and nomenclatural uncertainties in each of these genera are discussed.

Taxonomic Revision of the Genus Podagrostis (Poaceae, Pooideae, Poeae)

A taxonomic revision of the genus Podagrostis (Griseb.) Scribn. & Merr. is presented, including two new combinations: P. meridensis (Luces) A. M. Molina & Rúgolo and P. novogaliciana (McVaugh) A. M. Molina & Rúgolo. Podagrostis bacillata (Hack.) Sylvester & Soreng constitutes a new report for Colombia. Information regarding synonymy, geographical distribution, habitat, iconography, and vernacular names is provided along with complete illustrations and a list of additional specimens examined. A key for the identification of the species is included as well as a comparative table based on macro- and micromorphological data. The anatomical character of Trichodium net on lemma epidermis, as well as its diagnostic value, is discussed in species of Podagrostis and most related genera (e.g., Agrostis L., Chaetotropis Kunth, and Polypogon Desf., among others).

Most important carpological anatomical characters in the taxonomy of Apiaceae

Carpological anatomical characters are important in the taxonomy of Apiaceae. At present, it is necessary to include them in any critical revision of taxa of every rank, to characterize species and genera and to identify groups of related taxa. To create standardized descriptions of these taxa, we have compiled a list of taxonomically important fruit anatomical characters. This list includes 15 characters and 79 character states. Fruit anatomical characters are often constant in genera and can characterize genera or groups of closely related genera. However, the same anatomical character can occur in distant taxa. All anatomical characters are illustrated on schematic transverse sections of mericarps or in photographs.

Variasi Karakter Anatomis Talus Padina australis Hauck 1887 (Dictyotales, Phaeophycota) di Pantai Karang Tengah Kabupaten Cilacap

ABSTRAK: Karakter anatomi dapat digunakan untuk menentukan produktivitas kandungan alginat rumput laut P. australis Hauck. Penelitian ini bertujuan mengetahui variasi karakter anatomis talus Padina australis Hauck. Pengambilan sampel dengan purposive random sampling di pantai Karang Tengah, Kabupaten Cilacap. Persiapan mikroskopis dengan metode parafin dengan safranin 1% dalam alkohol 70%. Parameter yang diamati meliputi ketebalan epidermis, ukuran sel medula, ketebalan talus, dan ukuran tetra sporangia. Pengamatan struktur anatomi secara deskriptif dengan membandingkan karakter anatomi P. australis Hauck pada garis konsentris. Data karakter anatomi dianalisis dengan Uji-t dengan tingkat kepercayaan 95%. Hasil penelitian menunjukkan bahwa variasi karakter anatomi talus dengan garis konsentris 5 memiliki ketebalan epidermis adaxial dan abaxial masing-masing adalah 20,05 μm dan 12,55 μm, sedangkan pada garis konsentris 7 memiliki ketebalan 28,33 μm dan 18 μm. Ketebalan talus dengan garis konsentris 5 dan 7 masing-masing adalah 99,44 μm, 114,77 μm. Diameter meduler dengan garis konsentris 5 dan 7 masing-masing adalah 37,88 μm, 45,5 μm. Diameter rata-rata tetra sporangia masing-masing dengan garis konsentris 5 dan 7 yaitu 25,66 μm, 35,66 μm. Talus P. australis Hauck berdasarkan garis konsentris 5 dan 7 memiliki perbedaan ukuran pada ketebalan epidermis adaxial, ketebalan epidermis abaxial, ketebalan talus, diameter meduler, dan diameter tetra sporangia. Implikasi penelitian ini sebagai database pada data dasar karakter anatomi P. australis yang dikaitkan denggan produktsi alginat dan data dasar taksonomi. ABSTRACT: Anatomical character data can be used to determine the productivity of P. australis Hauck's seaweed alginate content. Each thallus has a thallus size and the number of varied concentric lines. The aim of the research is to determine the anatomical characteristics of the Padina australis Hauck thallus. Seaweed sampling by purposive random sampling technique in Karang Tengah beach, Cilacap regency. Preparation of microscopic by paraffin method with safranin 1% in 70% alcohol. Parameters observed included thickness of the epidermis, size of a medullary cell, the thickness of thallus, and the size of tetra sporangia. The anatomical character data was analyzed by t-Test with a 95% confidence level. The result of the research showed that the anatomical character of the thallus with concentric line 5 had a thickness of adaxial and abaxial epidermis respectively is 20.05 μm and 12.55 μm, whereas in concentric line 7 had a thickness of 28.33 μm and 18 μm. The thickness of the thallus with concentric lines 5 and 7 respectively is 99.44 μm, 114.77 μm. Medullary diameter with concentric lines 5 and 7 respectively is 37,88 μm, 45,5 μm. The average diameter of tetra sporangia with concentric lines 5 and 7 respectively that is 25,66 μm, 35,66 μm. Thallus of P. australis Hauck based on concentric lines 5 and 7 has differences of sizes on the adaxial epidermal thickness, thickness of the abaxial epidermis, the thickness of the thallus, medullary diameter, and diameter of tetra sporangia. The implications anatomical characters associated with alginate productivity and taxonomic baseline data.

Clinical Survey on Preputial Prolapse in Gir Bulls

A clinical survey was carried out to assess the etiopathology of preputial prolapse in Gir bulls at 41 Gaushalas of the Saurashtra region in Gujarat. A total of 202 Gir bulls under the breeding program were surveyed. The mean age of Gir bulls was recorded as 5.71 years, which ranged from 2.5 to 12 years. Preputial prolapse was diagnosed in all the surveyed Gir bulls. The first degree preputial prolapse was common in all the bulls because of the breed anatomical character. Out of the 202 breeding bulls, 30 (14.85%) bulls had pathological preputial prolapse, which varied between 2nd and 4th degrees. Among the 30 affected bulls, 12 (40 %) bulls had preputial prolapse of 2nd degree, 6 (20 %) bulls had 3rd degree of preputial prolapse and 12 (40 %) bulls had 4th degree of preputial prolapse. Traumatic injury to prepuce, followed by its contamination, was the main etiology for the preputial prolapse and its aggravations in all the animals. All Gir bulls had common anatomical / structural deformities of the preputial sheath.

The Contribution of Calcium to Changes in Leaf Anatomical Character of Oil Palm Seedlings (Elaeis guineensis Jacq.) under Drought Stress

The research was purposed (1) to know the effects of drought stress on changes in leaf anatomical character of oil palm seedlings (2) to know the contribution of calcium in cell compactness and increase the structural strength of leaf tissue so that oil palm seedlings were more tolerant to drought stress. This experiment was laid out following a split plot design with three blocks as replication. Main plot consists of drought stress levels, that are field capacity (FTSW 1.00), moderate drought stress (FTSW 0.35) and severe drought stress (FTSW 0.15). The fraction of transpirable soil water (FTSW) is a method for evaluating gradually increasing drought stress based on the amount of water loss due to transpiration. Meanwhile, subplot consists of four doses of calcium (Ca), that are of 0.0 g/seedlings, 0.04 g/seedlings, 0.08 g/seedlings, and 0.12 g/seedlings. Calcium fertilizer used is calcium sulfate (CaSO4) pure analysis. Leaf anatomical character was observed including the epidermal length and epidermal width; hypodermal length and hypodermal width; palisade cell length and palisade cell width; sponge cell length and sponge cell width; mesophyll tissue thickness; xylem and phloem diameter. The results showed that moderate and severe drought stress reduced epidermal cell length, upper hypodermal cell width, mesophyll thickness, palisade width and phloem diameter of leaf vessels. The applications of calcium to the leaf of oil palm seedlings under drought stresses were able to increased in the sponge cell length at a Ca dosage of 0.04 g/seedlings; increased lower hypodermal width and diameter phloem at a Ca dosage of 0.04 g/seedlings; and increased diameter xylem of leaves vessel at a Ca dosage of 0.12 g/seedlings.

Morphology and Anatomy Characteristic of Pisang Awak (Musa paradisiaca cv. Awak) in West Kalimantan

<p class="IsiAbstrakIndo"><span lang="EN-GB">Indonesia is the origin and center of diversity of banana. One of an edible banana in Indonesia is Pisang Awak (</span><em><span lang="EN-GB">Musa paradisiaca</span></em><span lang="EN-GB"> cv. Awak ). In West Kalimantan, the ripe Pisang Awak has been processed into </span><em><span lang="EN-GB">sale</span></em><span lang="EN-GB"> (dried banana). The aims of this research were to describe the morphological and anatomical character of Pisang Awak in West Kalimantan, Indonesia. In this study, Pisang Awak were collected from Padang Tikar I village, Batu Ampar Sub-district, Kubu Raya district, West Kalimantan. Morphological characterizations were conducted by following the instruction on Descriptors for Banana (</span><em><span lang="EN-GB">Musa</span></em><span lang="EN-GB"> spp.) from IPGRI. The root, leaf blade, and petiole were fixed in FAA solution. Root, leaf, and petiole anatomy preparats were made by paraffin method. The lamina of Pisang Awak consisted of adaxial epidermis, two hypodermis layers, two palisade layers, spongy layer, bundle sheath cell, abaxial epidermis, laticifer. The petiole of Pisang Awak composed of three tissue systems, i.e., epidermis layer, parenchyma tissue and vascular tissue. The root of Pisang Awak consists of two epidermis layers, parenchyma and vascular cylinder. In the future, morphological and anatomical character in Pisang Awak could be applied as the basis of information for breeding programs of banana cultivars and classification.</span></p>

Karakter Antomi Daun Tumbuhan Mangrove Akibat Pencemaran di Hutan Mangrove Kabupaten Cilacap

This research was aimed to observe the anatomical character of mangrove plants leave and examine the differences from leaves anatomical character of mangroves in Donan river and Tritih forest in Cilacap regency as an self-adaptation indicator. The method which is used was survey method by collecting leaves sample of each mangrove plants. The sample was preparated using embedding method, and stained with safranin 1% disolved in alcohol 70%. The parameter of variable which is observed in leaves anatomical character are thickness of leaves, leaves mesophyll thickness, cuticle thickness, stomata length size, and amount of stomata and trichome per leaves unit area. The data were analyzed with t-test for examining anatomical character difference. The results showed that four usual types of mangrove plants such as Achantus ilicifolius, Rhizospora mucronata, Aegiceros corniculatum, and Bruguiera gymnorrhiza have similar leaves anatomical structure. The anatomical character from polluted habitat were different from unpolluted habitat. This differences was showed in leaves mesophyll thickness, cuticle thickness, stomata length size, and amount of stomata and trichome per mm2 leaves unit area.