Development of new HPLC method for identification of metabolic degradation of N-pyrrolylhydrazide hydrazones with determined MAO- B activity in cellular cultures

In this research, a new rapid PR- HPLC method was developed for the determination of metabolites in isolated rat hapatocytes. The chromatographic parameters, including the stationary and mobile phases, outlet pressure, temperature and flow rate, were optimized. The method identified two initial from the synthesis molecules in higher concentration and one new unidentified structure as products of the hepatocytic processing of the evaluated analyte. The results identified as first step of metabolism the hydrolysis of the hydrazone group. Further investigations should be aimed into determining the next metabolic transformations, predicted by the in silico application of the web server SMARTCyp.

A Mathematical Model on Linkage Leakage in Sewage Pipes Laid in a Porous Ground Using Computation Fluid Dynamics.

This study describes the linkage leakage in sewage pipes through a porous media using computational fluid dynamics with the presence of one leak through fluid simulations using the Ansys fluent 17.2 commercial software based on standard k-ε model under steady-state condition. The pipe section is three-dimensional with a pipe length of 40 mm, a pipe diameter of 20 mm, and leak orifice diameter of 2 mm with a porous media of length 25 mm and width 30 mm. The interest of this study was to reduce the rate of sewage leakage in pipes laid underground by use computational fluid dynamics. The simulation results obtained shows that when the flow is subjected to an outlet pressure between 100000 Pa to 275000 Pa the sewage leaks at pressures of 99499 Pa to 278799.8 Pa indicating that increase of outlet pressures increases the pressure at the leak point and also an increase in the inlet velocity resulted into an increase of velocity at the leak point and no significant change in sewage flow rate with increased inlet velocities. Therefore, monitoring of the pressure and velocity fields along the pipeline is an extremely important tool to identify leaks since these fields are affected by perturbations both before the leak point and after the leak point.

Design and Experiment of Hydraulic Scouring System of Wide-Width Lotus Root Digging Machine

In response to the problems of small working width and low operating efficiency of existing hydraulic scouring lotus root harvesters, a wide-width hydraulic scouring system was designed based on a wide-width self-propelled lotus root harvester. The main parameters of the key components were determined through theoretical analysis of the water flow energy of the hydraulic scouring system pipelines. An experimental study was also carried out on the main factors affecting the working performance of this hydraulic scouring system. Through hydrodynamic simulation tests, the effect of nozzle type and constriction section structure on the turbulence intensity at the nozzle outlet and the pressure loss per unit mass of fluid between the nozzle inlet and outlet sections were compared and analysed. The test yielded conical-cylindrical nozzle geometry parameters for nozzle inlet diameter of 40 mm, shrinkage angle of 30°, nozzle outlet straight section length of 20 mm, nozzle outlet diameter of 16 mm, the nozzle had better flushing performance. Single-factor tests were carried out with nozzle outlet pressure, scouring angle and nozzle height from the mud surface as influencing factors. Based on the optimum effective scour depth, a three-factor, three-level Box–Behnken central combination design test was completed. The primary and secondary factors affecting the effective scouring depth were obtained in the following order: nozzle height from the mud surface, nozzle outlet pressure, and scouring angle. Finally, the performance test of the hydraulic scouring system was completed. Results showed that when the nozzle outlet pressure of 0.30 MPa, the scouring angle of 60° and the nozzle height from the mud surface of 0 mm, the effective scouring depth was 395 mm, the lotus root floating rate was 90% and the damage rate was 5%, which meet the requirements of lotus root harvesting operations.

Influences of Pressure ratio and Fluid Temperature on overall Cooling Performances of Ribbed Channel with Film Holes

The previous experiments of overall cooling performances were most conducted using simplified models and under the similar temperature ratio of mainstream to cooling air with real gas turbine operations, and ambient outlet pressure. To discuss the reliability of this type of experimental data, this paper exhibits two series of numerical simulations. Using a real E3 blade as model, which has two-pass rib-roughened channel with inclined film holes, numerical simulations are carried out at the same temperature ratio and pressure ratio, but different fluid temperatures including mainstream and cooling air, and different outlet pressure. The numerical results reveal two important conclusions: 1) At the same outlet pressure, the overall cooling effectiveness on PS is not sensitive to the fluid temperatures, but on SS in the region between two rows of film holes, a higher fluid temperature corresponds to a higher cooling effectiveness. 2) At the same pressure ratio of inlet to outlet, the overall cooling effectiveness on PS and SS is not sensitive to the outlet pressure and fluid temperature.

Investigation on Clocking Effect of Diffuser in a Multi-Stage Centrifugal Pump

The clocking effect is an important phenomenon in the multi-stage Rotating machinery. In order to master the rules and mechanism of diffuser clocking effect on the performance of multi-stage centrifugal pump, the orthogonal tests were applied to design the test scheme. The energy performance and outlet pressure pulsation of a multi-stage centrifugal pump with different diffuser clocking positions were synchronously measured. It was found that the diffuser clocking position had little influence on the energy performance, but had an obvious effect on the outlet pressure pulsation. When the diffuser clocking positions were 0°, 30°, 30° and 30° (CL4), the effective value of outlet pressure pulsation and its amplitude at the main frequency (Impeller Rotation Frequency) were decreased the most, approximately 27.2 % and 38.5 % ,respectively. The CFD method was used to simulate the unsteady flow in the pump with the optimal diffuser clocking position (CL4) and without diffuser clocking position (CL1) respectively to reveal the mechanism of diffuser clocking effect. The simulation results showed that the change of diffuser clocking position can improve the inlet and outlet velocity distribution and reduce the area of high turbulent kinetic energy and the number of cores in the outlet flow passage, which is beneficial to the operation stability of the pump. Compared with the CL1, the hydraulic loss in the four diffusers was reduced by 2.43 %, 12.15 %, 11.43 % and 13.19 % respectively under the optimal diffuser clocking scheme (CL4), and the total reduction of hydraulic loss is about 1.11 % of the pump head.

On a Casson Fluid Motion: Nonuniform Width Symmetric Channel and Peristaltic Flows

Widely used for modeling biological fluids flows—in particular, blood vessel flows—a Casson flow is studied in a symmetric channel for which the aspect ratio enables one to use the lubrication approximation. Two flow driving conditions are prescribed: inlet–outlet pressure difference and peristaltic oscillations of the vessel walls. In both cases, starting from mass and momentum balance and using lubrication approximation, we investigate the conditions to be imposed on the driving mechanisms so that the inner plug does not come in touch with the walls. The study of the peristaltic flow is of great importance in view of its applications in physiology (including microcirculation applications).

Rotordynamic Characteristics of the Straight-Through Labyrinth Seal Based On the Applicability Analysis of Leakage Models Using Bulk-Flow Method

The fluid excitation induced by the labyrinth seal would deteriorate the stability of turbomachinery shaft. Developing an accurate and rapid prediction approach is crucial for the analysis of the fluid excitation rotordynamics of the labyrinth seal. The objective of this study is to analyze the applicability of leakage models using Bulk-Flow method and investigate the factors affecting the rotordynamic characteristics of the labyrinth seal. An elliptical orbit for rotor whirling was assumed in the one-control-volume Bulk-Flow model considering an isentropic process to predict the frequency-dependent rotordynamic coefficients of the labyrinth seal. The optimal leakage model was determined by comprehensively analyzing the applicability of 72 leakage models. Employing the optimal leakage model in the Bulk-Flow method, the effects of sealing clearance, pressure ratio, preswirl ratio and rotational speed on the rotordynamic characteristics of the labyrinth seal were investigated. The conclusions show that the Bulk-Flow method has an average prediction error of around 10% for the leakage flow rate, cross-coupled stiffness and direct damping when equipped with the optimal leakage model. Increasing preswirl ratio has a significantly destabilizing effect on the rotor stability, while the influence of increasing rotational speed is strongly related to preswirl direction. The effective damping of the labyrinth seal is sensitive to the inlet pressure, but insensitive to the outlet pressure and sealing clearance. The crossover frequency is almost impervious to the inlet pressure, outlet pressure and sealing clearance.

A Semi-Empirical Fluid Dynamic Model of a Vacuum Microgripper Based on CFD Analysis

Vacuum microgrippers are devices used to handle and manipulate small objects. Despite their simple working principle and low cost, they show low efficiency in detaching performance, especially when the object to be grasped is very small. In this work, a particular design for vacuum microgrippers with an incorporated automatic release tool is considered. The final goal of this study was to present a numerical model that can supply reliable estimates of the aerodynamic force acting on the release tool and of the air flow rate inside the gripper as a function of geometric parameters and the outlet pressure value. A complete CFD analysis of a simplified model of the device is presented. Grid independence analysis was also performed to define a suitable grid and guarantee a good trade-off between accuracy and computing time. According to Design of Experiments (DOE) techniques, 81 simulations were performed, changing the values of the outlet pressure (p2), the body inner diameter (D), the lateral holes’ diameter (d) and the releasing mass length (L). Every design variable could assume three different values. Linear regression, based on the least square method, was employed to determine mass flow rate and lifting force empirical correlations.

Experimental Investigation of the Effects of Arterial Geometries in Different Severities of Symmetrical Stenosis on Pressure Drop and Pump Power

Biomedical studies is among the multidisciplinary studies attracting most interest in recent years. Blood and vessel interactions and consequent hemodynamic effects cause cardiovascular diseases. A testing setup constituted by a peristaltic pump (similar to the heart mechanism) system was installed. The purpose of the experimental study presented is to investigate the effect, pressure drop, peristaltic pump inlet and outlet pressure and most importantly, the amount of power consumed by the peristaltic pump regarding arterial stenosis severity with varying areal stenosis percentages. The tests were performed for the pulse values from 54 to 168 bpm by setting up models with 0%, 60%, 70% and 80% symmetrical stenosis severities. In the study, the pressure difference in the test area increased concomitantly with elevated pulse value and increased stenosis severity. This situation revealed that as the intensity of narrowing increases in vessels, the narrowing space differential pressure increases, and this amount increases even more with increased exertion. The pressure at the peristaltic pump outlet increased concomitantly with elevated pulse value and increased stenosis severity. The peristaltic pump overworked to overcome the increased differential pressure related to the increased pulse value and stenosis severity. This result of the experimental data reveals the necessity to avoid activities requiring high pulse in human arteries similarly with a high percentage of stenosis.