Nigella sativa Oil Preserved Anxiety-Like, Motor and Memory Related Behaviours and Neuronal Integrity in Dichlorvos Induced Acetyl Cholinesterase Inhibitions in Rats

Organophosphates are irreversible cholinesterase (ChE) inhibitors with neurological consequences, and there is not yet an effective antidote. Here, we investigated the effects of Nigella sativa oil (NSO) on the ChE inhibition, neurobehavioural and histopathological changes following dichlorvos (DDVP) ingestions in rats. Thirty-two male Wistar rats were randomised into four groups, receiving 1 ml/kg of normal saline, 8.8 mg/kg of DDVP, 8.8 mg/kg of DDVP and 1 ml/kg of NSO, and 1 ml/kg of NSO only respectively, for 14 consecutive days. Locomotor, anxiety-like behaviours and spatial working memory were assessed on the 14th day, using open field (OF), Y-maze and modified elevated plus maze paradigms. The rats were euthanized on the 15th day and the brains excised; three brains were fixed for histopathology, and the other five prepared for biochemical analysis of acetyl cholinesterase (AChE). DDVP exposure caused significant reductions in frontal, amygdala and cerebella AChE activity, spontaneous alternations, line crossing and rearing frequencies and time in centre square, and caused increase in freezing period, transfer latency and necrotic-like cells. NSO intervention was able to reverse DDVP effects on AChE activities, explorative, locomotor, anxiety and spatial memory behaviours in co-exposed rats. It also preserved the histological integrity of the investigated brain regions. It can be concluded that NSO, may be potent against organophosphates induced neurotoxicity and their neurobehavioural consequences through the modulation of AChE activities.

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Region-specific interneuron demyelination and heightened anxiety-like behavior induced by adolescent binge alcohol treatment

Acta Neuropathologica Communications ◽

10.1186/s40478-019-0829-9 ◽

2019 ◽

Vol 7 (1) ◽

Cited By ~ 3

Author(s):

James Rice ◽

Laurence Coutellier ◽

Jeffrey L. Weiner ◽

Chen Gu

Keyword(s):

Spatial Working Memory ◽

Early Adulthood ◽

Alcohol Treatment ◽

Brain Regions ◽

Behavioral Changes ◽

Y Maze ◽

Plus Maze ◽

Public Health Challenge ◽

Temporal And Spatial ◽

Binge Ethanol

Abstract Adolescent binge drinking represents a major public health challenge and can lead to persistent neurological and mental conditions, but the underlying pathogenic mechanisms remain poorly understood. Using a mouse model of adolescent binge ethanol treatment (ABET), we found that this treatment induced behavioral changes associated with demyelination in different brain regions. After ABET, adolescent mice exhibited anxiogenic behaviors with no change in locomotion on the elevated plus maze, and impaired spatial memory indicated by a significant reduction in spontaneous alternation in the Y maze test. Both effects persisted into adulthood. Anatomical studies further showed that ABET induced a significant reduction of parvalbumin-positive (PV+) GABAergic interneurons and myelin density in the hippocampus and medial prefrontal cortex (mPFC). While these deficits in PV+ interneurons and myelin persisted into early adulthood in the hippocampus, the myelin density recovered in the mPFC. Moreover, whereas ABET mainly damaged myelin of PV+ axons in the hippocampus, it primarily damaged myelin of PV-negative axons in the mPFC. Thus, our findings reveal that an adolescent binge alcohol treatment regimen disrupts spatial working memory, increases anxiety-like behaviors, and exerts unique temporal and spatial patterns of gray matter demyelination in the hippocampus and mPFC.

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Nigella sativa oil protected the hippocampus against Acetyl cholinesterase and oxidative dysfunctions-driven impaired working memory in rats

Bulletin of Faculty of Pharmacy, Cairo University ◽

10.21608/bfpc.2019.47261 ◽

2019 ◽

Vol 57 (1) ◽

pp. 25-34

Author(s):

Imam Aminu ◽

Alakoso Teslimat ◽

Williams Victoria ◽

Chengetanai Samson ◽

Oyewole Aboyeji ◽

...

Keyword(s):

Working Memory ◽

Nigella Sativa ◽

Acetyl Cholinesterase ◽

Nigella Sativa Oil

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The effect of black cumin oil (Nigella sativa) on the reproduction of male Wistar rats

BIOEDUPAT: Pattimura Journal of Biology and Learning ◽

10.30598/bioedupat.v1.i2.pp45-49 ◽

2021 ◽

Vol 1 (2) ◽

pp. 45-49

Author(s):

Syahran Wael ◽

Didik Wahyudi ◽

Tayeb Wael ◽

Zaid Mohamed Jaber

Keyword(s):

Wistar Rats ◽

Nigella Sativa ◽

Cellular Damage ◽

Control Group ◽

Control Group Design ◽

Black Cumin ◽

Group Design ◽

Nigella Sativa Oil ◽

Post Test ◽

Male Wistar Rats

Nigella sativa oil is antioxidant compound has the effect that serves to prevent cellular damage. The effect of Nigella sativa oil in the motility and total count of spermatozoa wistar rats. Experimental research with the design of post test only control group design. Wistar rats consists of 24 head and divided into 4 groups consist of control and treatment group. The control group was distilled. The first treatment was of Nigella sativa oil everydays as much with dose 150 mg/kgbw, 250 mg/kgbw, and 350 mg/kgbw, for 16 days, . Statistic test for motility and count of sperm wistar rats use the Kruskal-Wallis followed by Mann Whitney test. Avarage value of motility in the control (21.67Â±9.832) its lower than treatment. In dose 350 mg/kgbw its highest (52.33Â±13.292) compare in the treatment 250 mg/kgbw (40.67Â±17.512) and 150 mg/kgbw (30.67Â±8.165). avarage value of count sperm in the control (130.83Â±41.877) its lower than treatments. In dose 350 mg/kgbw its highest (199.67Â±23.480) compare in the treatment 250 mg/kgbw (187.50Â±74.538) and 150 mg/kgbw (140.83Â±32.568). Administration of Nigella sativa oil occur to enhancement motility and number of spermatozoa wistar rats.

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Chlorpyrifos- and Dichorfos-Induced Oxidative and Neurogenic Damage Elicits Neuro-Cognitive Deficits and Increases Anxiety-Like Behaviors in Wild-Type Rats

10.20944/preprints201810.0359.v1 ◽

2018 ◽

Author(s):

Aminu Imam ◽

Nafeesah Abdulkareem Sulaiman ◽

Aboyeji Lukuman Oyewole ◽

Samson Chengetanai ◽

Victoria Williams ◽

...

Keyword(s):

Spatial Working Memory ◽

Cell Loss ◽

The Body ◽

Fear Learning ◽

Cresyl Violet ◽

Organophosphate Poisoning ◽

Cognitive Behaviors ◽

Plus Maze ◽

Body Weights ◽

Male Wistar Rats

The mechanization of agricultural activities has led to indiscriminate deposition of toxic xenobiotics, including organophosphates in the biomes, and this has led to intoxication characterized with deleterious oxidative and neuronal changes. This study investigated the consequences of oxidative and neurogenic disruptions that follow exposure to two organophosphates, chlorpyrifos (CPF) and dichlorvos (DDVP) on neuro-cognitive performance and anxiety-like behaviors in rats Thirty-two adult male Wistar rats (150 – 170g) were randomly divided into 4 groups, orally exposed to normal saline (NS), DDVP (8.8mg/kg), CPF (14.9mg/kg) and DDVP+CPF for 14 consecutive days. On day 10 of exposures, anxiety-like behaviors and amygdala dependent fear learning were assessed using Open Field and Elevated Plus Maze paradigms respectively, while spatial working memory was assessed on day 14 in the Morris water maze paradigm, following 3 training trials each on days 11, 12 and 13. On day 15, the rats were euthanized, and their brains excised, hippocampus and amygdala removed, 5 of which were homogenized and centrifuged to analyze nitric  oxide (NO) metabolites, total reactive oxygen species (ROS), and acetylcholinesterase (AChE) activity, and the other three processed for histology (cresyl violet stain) and proliferative marker (Ki67 immunohistochemistry). Marked (p≤0.05) loss in body weight, AChE depletion, and overproduction of both NO and ROS were observed after repeated exposure to individual and combined doses of CPF and DDVP. Insults from DDVP exposure appeared more severe owing to the observed greater losses in the body weights of exposed rats. There was also a significant (p≤0.05) effect on the cognitive behaviors recorded from the exposed rats, and these deficits were related to the oxidative damage and neurogenic cell loss in the hippocampus and the amygdala of the exposed rats. Taken together, these results provided an insight that oxidative and neurogenic damages are central to the severity of neuro-cognitive dysfunction and increased anxiety-like behaviors that follow organophosphate poisoning.

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Conditioned place preference of kisspeptin-10

Reviews on Clinical Pharmacology and Drug Therapy ◽

10.17816/rcf19147-53 ◽

2021 ◽

Vol 19 (1) ◽

pp. 47-53

Author(s):

Ilia Yu. Tissen ◽

Polina A. Chepik ◽

Andrei A. Lebedev ◽

Leila A. Magarramova ◽

Eugenii R. Bychkov ◽

...

Keyword(s):

Conditioned Place Preference ◽

Open Field Test ◽

Physiological Saline ◽

Preference Test ◽

Brain Regions ◽

Place Preference ◽

Male Rats ◽

Plus Maze ◽

Male Wistar Rats ◽

Positive State

INTRODUCTION: Kisspeptins (KISS), a group of brain neuropeptides are involved in sexual behavior. KISS activate the hypothalamic neurons that synthesize gonadotropin releasing hormone. KISS was also detected in the limbic system. Earlier, we showed the activation of sexual motivation after the administration of kisspeptin-10 without increasing the level of testosterone in male rats, which suggests the extrahypothalamic effect of KISS. The aim of this work was to study the possibility of aquisition of conditioned place preference of kisspeptin-10, as well as to study the emotional and investigational behavior in rats after intranasal peptide administration. METHODS: Conditioned place preference test (CPP), open field test (OP) and elevated plus maze (EPM) were used in male Wistar rats. RESULTS: When studying CPP, animals spent 78.6 6.3% of the time in the chamber associated with the administration of KISS compared to control animals with administration of physiological saline (51.2% of the experiment time; p 0.05). After kisspeptin-10 administration locomotor activity was 2-fold increased (p 0.05), and the number of sniffings was 2-fold increased too (p 0.05). The parameters did not significantly differ in animals treated with kisspeptin or saline in PCL. CONCLUSION: Thus repeated intranasal administration of kisspeptin-10 induces the aquisition of CPP in rats. This suggests that kisspeptin-10 can cause activity in the reward system or the activation of brain regions associated with this system, which ultimately leads to the formation of an emotionally positive state.

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The Effect of Black Cumin (Nigella sativa) Oil Supplementation on Hematotoxicity Induced by Benzene in Gasoline Vapors from Gas Stations in Male Wistar Rats

Indian Journal of Forensic Medicine & Toxicology ◽

10.37506/ijfmt.v15i3.15810 ◽

2021 ◽

Keyword(s):

Wistar Rats ◽

Nigella Sativa ◽

Black Cumin ◽

Gas Stations ◽

Nigella Sativa Oil ◽

Male Wistar Rats

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Effects of dopaminergic drugs in the dorsal hippocampus of rats in the MK801-induced anxiolytic-like behavior

European Psychiatry ◽

10.1016/s0924-9338(11)72148-4 ◽

2011 ◽

Vol 26 (S2) ◽

pp. 441-441

Author(s):

M.R. Zarrindast ◽

M. Nasehi ◽

M. Pournaghshband

Keyword(s):

Dorsal Hippocampus ◽

Brain Regions ◽

Nmda Receptor Antagonist ◽

Main Mode ◽

Dopaminergic Drugs ◽

Excitatory Transmission ◽

Dorsal Hippocampal ◽

Test Parameters ◽

Plus Maze ◽

Male Wistar Rats

IntroductionExcitatory transmission through glutamate receptors constitutes the main mode of synaptic signaling in the brain regions that are critical for cognition such as learning and anxiety.ObjectivesThe possible involvement of dorsal hippocampal (intra-CA1) dopaminergic receptor mechanism on the anxiolytic-like response induced by NMDA receptor antagonist, MK801 has been investigated in the present study.MethodsThe male wistar rats were used and the elevated plus maze apparatus has been used to test parameters (%OAT, %OAE, locomotor activity, grooming, rereading and defection) of anxiety-like behaviors in the present study.ResultsThe data indicated that intra-CA1 administration of MK801 (2 μg/rat, intra-CA1) increased %OAT and %OAE but not other exploratory behaviors, indicating an anxiolytic-like response. Moreover, intra-CA1injection SCH23390 (0.25, 0.5 and 1 μg/rat) and sulpiride (0.25, 0.5 and 0.75 μg/rat) by itselves, 5 min before testing have no effect on exploratory behaviors. On the other hand, co-administration of ineffective dose of SCH23390 (0.5 μg/rat) with ineffective dose of MK801 (1 g/rat) increased %OAT but not other exploratory behaviors, suggestion anxiolytic-like behaviors. Furthermore, intra-CA1 administration of different doses of sulpiride (0.12, 0.5 and 0.75 μg/rat) 5 min before injection of effective dose of MK801 (2 μg/rat) decreased %OAT and %OAE but did not other exploratory behaviors induced by MK801.ConclusionThe results may indicate modulatory effect dopaminergic system of CA1 in the anxiolytic-like response induced by MK801.

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EFEK NIGELLA SATIVA OIL TERHADAP UKURAN DIAMETER ULKUS TRAUMATIKUS PADA MALE WISTAR RATS SECARA IN VIVO

ODONTO Dental Journal ◽

10.30659/odj.3.2.94-97 ◽

2016 ◽

Vol 3 (2) ◽

pp. 94

Author(s):

Linda Septiana ◽

Ratnawati Hendari ◽

Erwid Fatchur Rahman ◽

Diyah Fatmasari

Keyword(s):

Wistar Rats ◽

Nigella Sativa ◽

Pathological Condition ◽

Negative Control Group ◽

Negative Control ◽

Control Group ◽

Epithelial Tissue ◽

Nigella Sativa Oil ◽

Male Wistar Rats

Background: Ulcer is a pathological condition characterized by loss of epithelial tissue. Ulcer will experience healing within 2 weeks after trauma source is removed. Nigella sativa Oil has been known to heal wounds. The purpose of this study was to evaluate the effect of NSO on healing of ulcers in male wistar rats from the use of chemicals.Method: This study design was quasi-experimental methods. Ten male wistar rats were exposing the H2O2 on the mandibular anterior gingiva. divided into negatif control group and NSO group. NSO group treated twice daily for 10 days. Observaton wound size was measured on 0, 3,7, 10 days. The results were analayzed with Repeated Anova test and LSD test.Result: The observations difference diameter of traumatic ulcer negative control group and NSO on 0-10 days was 1.458 mm and 2.182 mm. The results of data analysis showed that there are significant differences (p<0,05) between negatif control group and NSO.Conclusion: NSO has an influence of the size reduction of the diameter of traumatic ulcers.

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