Corrigendum to: Computational Modeling of Environmental Co-exposure on Oil-Derived Hydrocarbon Overload by Using Substrate-Specific Transport Protein (TodX) with Graphene Nanostructures

Due to an oversight of the publisher, Page no 2310 was missing in the published paper and page no 2311 repeated twice in the article entitled “Computational Modeling of Environmental Co-exposure on Oil-Derived Hydrocarbon Overload by Using Substrate-Specific Transport Protein (TodX) with Graphene Nanostructures, 2020, 20(25), 2308-2325 [1]. The page no 2310 is added in the article and the repetition of page no 2311 is corrected. <p> The original article can be found online at https://doi.org/10.2174/1568026620666200820145412

Computational Modeling of Environmental Co-exposure on Oil-Derived Hydrocarbon Overload by Using Substrate-Specific Transport Protein (TodX) with Graphene Nanostructures

Background: Bioremediation is a biotechnology field that uses living organisms to remove contaminants from soil and water; therefore, they could be used to treat oil spills from the environment. Methods: Herein, we present a new mechanistic approach combining Molecular Docking Simulation and Density Functional Theory to modeling the bioremediation-based nanointeractions of a heterogeneous mixture of oil-derived hydrocarbons by using pristine and oxidized graphene nanostructures and the substrate-specific transport protein (TodX) from Pseudomonas putida. Results: The theoretical evidences pointing that the binding interactions are mainly based on noncovalent bonds characteristic of physical adsorption mechanism mimicking the “Trojan-horse effect”. Conclusion: These results open new horizons to improve bioremediation strategies in over-saturation conditions against oil-spills and expanding the use of nanotechnologies in the context of environmental modeling health and safety.

Kinesin-1 motility traced by an activity-based precipitating dye

Kinesin-1 is a processive motor protein that uses ATP-derived energy to transport a variety of intracellular cargoes toward the cell periphery. As tracks for cargo delivery, kinesin-1 uses a subset of microtubules within the dense microtubule network. It is still debated what defines the specific binding of kinesin-1 to a subset of microtubules. Therefore, the ability to visualize and monitor kinesin transport in live cells is critical to study the myriad of functions associated with cargo trafficking. Herein we report the discovery of a fluorogenic small molecule substrate for kinesin-1 that yields a precipitating dye. The activity of kinesin-1 thus leaves a fluorescent trail along its walking path and can be visualized without loss of signal due to diffusion. Kinesin-1 specific transport of cargo from the Golgi appears as trails of fluorescence over time.

A Modern Mechanisam and Functioning of Bus Transit using Rfid and Gps Based System

Right now present transport a framework which kept stationary at the transport stand and framework which kept at transport that can adequately assist general society with participating in transport transportation offices to its fullest. A transport that is coming toward the transport – stop is recognized by this traveller through one of a kind traveller card the subtleties of that specific transport is given to the traveller in plain view at transport stand. Transport area recognized utilizing GPS and same will be declare in Bus This data send to next bus station for travellers. The transport distinguishing proof procedure includes use of Radio Frequency innovation and transport subtleties are reported by Voice and showed in screen unit. Subtleties incorporate the specific situation of the transport, seat accessibility and absolute number of travellers. This data can be shown on screen or can be given as sound yield. Here both transport and transport stop is interconnected through IOT, here we use MQTT convention for correspondence.

Coatopathies: Genetic Disorders of Protein Coats

Protein coats are supramolecular complexes that assemble on the cytosolic face of membranes to promote cargo sorting and transport carrier formation in the endomembrane system of eukaryotic cells. Several types of protein coats have been described, including COPI, COPII, AP-1, AP-2, AP-3, AP-4, AP-5, and retromer, which operate at different stages of the endomembrane system. Defects in these coats impair specific transport pathways, compromising the function and viability of the cells. In humans, mutations in subunits of these coats cause various congenital diseases that are collectively referred to as coatopathies. In this article, we review the fundamental properties of protein coats and the diseases that result from mutation of their constituent subunits.

The allosteric mechanism of substrate-specific transport in SLC6 is mediated by a volumetric sensor

Neurotransmitter:sodium symporters (NSSs) in the SLC6 family terminate neurotransmission by coupling the thermodynamically favorable transport of ions to the thermodynamically unfavorable transport of neurotransmitter back into presynaptic neurons. Results from many structural, functional, and computational studies on LeuT, a bacterial NSS homolog, have provided critical insight into the mechanism of sodium-coupled transport, but the mechanism underlying substrate-specific transport rates is still not understood. We present a combination of molecular dynamics simulations, single-molecule fluorescence resonance energy transfer (smFRET) imaging, and measurements of Na+ binding and substrate transport that reveals an allosteric substrate specificity mechanism. In this mechanism, residues F259 and I359 in the substrate binding pocket couple the binding of substrate to Na+ release from the Na2 site by allosterically modulating the stability of a partially open, inward-facing state. We propose a model for transport selectivity in which residues F259 and I359 act as a volumetric sensor that inhibits the transport of bulky amino acids.

The allosteric mechanism of substrate-specific transport in SLC6 is mediated by a volumetric sensor

Neurotransmitter:sodium symporters (NSS) in the SLC6 family terminate neurotransmission by coupling the thermodynamically favorable transport of ions to the thermodynamically unfavorable transport of neurotransmitter back into presynaptic neurons. While a combination of structural, functional, and computational studies on LeuT, a bacterial NSS homolog, has provided critical insight into the mechanism of sodium-coupled transport, the mechanism underlying substrate-specific transport rates is still not understood. We present a combination of MD simulations, single-molecule FRET imaging, and measurements of Na+ binding and substrate transport that reveal an allosteric mechanism in which residues F259 and I359 in the substrate binding pocket couple substrate binding to Na+ release from the Na2 site through allosteric modulation of the stability of a partially-open, inward-facing state. We propose a new model for transport selectivity in which the two residues act as a volumetric sensor that inhibits the transport of bulky amino acids.

BIAYA OPERASIONAL KENDARAAN PADA DINAS PERUMAHAN RAKYAT KAWASAN PERMUKIMAN DAN DINAS PEKERJAAN UMUM PENATAAN RUANG PROVINSI ACEH

The employee transportation service is an application of traffic management using vanpooling. Vanpooling is an activity of using a vehicle together or sharing a car on the way so that more than one person is in the van in one travel route. Vanpooling is able to reduce the use of private vehicles to the center of the activity with a fleet of special transport employees. The potential for employee specific transport is enormous and 83% agree with the implementation of the traffic management. The employee specific operating freight operational systems are determinations with residential area corridors and passing major road routes at the address of each employee. In the operation of a transport both personal, general and special, not apart from the vehicles operating costs (VOC). Therefore, an analysis of the need for the development of special transportation of employees in the Dinas Perumahan Rakyat Kawasan Permukiman dan Dinas Pekerjaan Umum Penataan Ruang Province of Aceh. The method used in this study is descriptive - quantitative, by distributing questionnaires to obtain quantitative data in determining VOC. The results of the VOC of private and special vehicles are summarized in a descriptive conclusion. The result of the research shows that employee characteristics of the data are processed to determine vehicle operating cost of private vehicles as well as the special transport vehicles of employees. The overall VOC of private vehicles is Rp. 2.486.377.640, while the value VOC of employee specific transport vehicles is Rp. 685.043.840.