Bacterial marginolactones trigger formation of algal gloeocapsoids, protective aggregates on the verge of multicellularity

Photosynthetic microorganisms including the green alga Chlamydomonas reinhardtii are essential to terrestrial habitats as they start the carbon cycle by conversion of CO2 to energy-rich organic carbohydrates. Terrestrial habitats are densely populated, and hence, microbial interactions mediated by natural products are inevitable. We previously discovered such an interaction between Streptomyces iranensis releasing the marginolactone azalomycin F in the presence of C. reinhardtii. Whether the alga senses and reacts to azalomycin F remained unknown. Here, we report that sublethal concentrations of azalomycin F trigger the formation of a protective multicellular structure by C. reinhardtii, which we named gloeocapsoid. Gloeocapsoids contain several cells which share multiple cell membranes and cell walls and are surrounded by a spacious matrix consisting of acidic polysaccharides. After azalomycin F removal, gloeocapsoid aggregates readily disassemble, and single cells are released. The presence of marginolactone biosynthesis gene clusters in numerous streptomycetes, their ubiquity in soil, and our observation that other marginolactones such as desertomycin A and monazomycin also trigger the formation of gloeocapsoids suggests a cross-kingdom competition with ecological relevance. Furthermore, gloeocapsoids allow for the survival of C. reinhardtii at alkaline pH and otherwise lethal concentrations of azalomycin F. Their structure and polysaccharide matrix may be ancestral to the complex mucilage formed by multicellular members of the Chlamydomonadales such as Eudorina and Volvox. Our finding suggests that multicellularity may have evolved to endure the presence of harmful competing bacteria. Additionally, it underlines the importance of natural products as microbial cues, which initiate interesting ecological scenarios of attack and counter defense.

Single-cell profiling of tuberculosis lung granulomas reveals functional lymphocyte signatures of bacterial control

In humans and nonhuman primates, Mycobacterium tuberculosis lung infection yields a complex multicellular structure—the tuberculosis granuloma. All granulomas are not equivalent, however, even within the same host: in some, local immune activity promotes bacterial clearance, while in others, it allows persistence or outgrowth. Here, we used single-cell RNA-sequencing to define holistically cellular responses associated with control in cynomolgus macaques. Granulomas that facilitated bacterial killing contained significantly higher proportions of CD4+ and CD8+ T cells expressing hybrid Type1-Type17 immune responses or stem-like features and CD8-enriched T cells with specific cytotoxic functions; failure to control correlated with mast cell, plasma cell and fibroblast abundance. Co-registering these data with serial PET-CT imaging suggests that a degree of early immune control can be achieved through cytotoxic activity, but that more robust restriction only arises after the priming of specific adaptive immune responses, defining new targets for vaccination and treatment.

Life cycle of Bacillus subtilis population: biofilm life cycle generation and response to environmental pH

Microbial populations are ubiquitous and some form a robust, multicellular structure called a biofilm that protects the cells from environmental damage. The biofilm life cycle is critical for controlling the population of important target microbes (both pathogenic and beneficial). Here we show that the hysteresis of Bacillus subtilis cell-type regulation with respect to auto-inducing signal strength leads to the life cycle of the cell population. We investigate life cycle generation and its dependence on environmental conditions by quantitative analysis of cyclically expanding, concentric circular colonies. Next, we construct an input/output model that controls cell types in response to environmental conditions and signal density. On the basis of this model, we propose a life cycle generation model for cell populations. The proposed model will widely predict biofilm-related phenomena and provide the basis for the description of highly self-regulating multicellular systems.

Lateral Crushing and Energy Absorption Behavior of Multicellular Tube Structures

Thin-walled metallic tubular elements are extensively employed as an impact energy attenuator in modern vehicles owing to light weight, easy fabrication and average cost. Besides, the novel multi-cell tubular structures have superior energy absorption characteristics related to a conventional simple cell tube. In this research article, the finite element simulation of thin-walled aluminium alloy extruded multicellular structure under lateral impact loading is investigated. Nonlinear impact simulations were performed on multicellular tubes of various configurations using finite element ABAQUS/CAE explicit code. From the outcomes attained, the energy absorption capability of various multicellular tube structures were compared and it shows that multicellular tubes have more remarkable than that of traditional simple cell tubes. Moreover, square shaped multicellular structure tube were retained as most prominent for higher energy absorption. This type of multicellular tubes was found to be effective one to improve the lateral crashworthiness performance

Going with the Flo: The Role of Flo11-Dependent and Independent Interactions in Yeast Mat Formation

Strains of the bakers’ yeast Saccharomyces cerevisiae that are able to generate a multicellular structure called a mat on low percentage (0.3%) agar plates are given a selective advantage over strains that cannot exhibit this phenotype. This environment may exhibit some similarities to the rotting fruit on which S. cerevisiae often grows in nature. Mat formation occurs when the cells spread over the plate as they grow, and cells in the center of the biofilm aggregate to form multicellular structures that resemble a floral pattern. This multicellular behavior is dependent on the cell surface flocculin Flo11. This review covers recent information on the structure of Flo11 and how this likely impacts mat formation as well as how variegated expression of Flo11 influences mat formation. Finally, it also discusses several Flo11-independent genetic factors that control mat formation, such as vacuolar protein sorting (VPS) genes, cell wall signaling components, and heat shock proteins.

The pericyte–glia interface at the blood–brain barrier

The cerebrovasculature is a multicellular structure with varying rheological and permeability properties. The outer wall of the brain capillary endothelium is enclosed by pericytes and astrocyte end feet, anatomically assembled to guarantee barrier functions. We, here, focus on the pericyte modifications occurring in disease conditions, reviewing evidence supporting the interplay amongst pericytes, the endothelium, and glial cells in health and pathology. Deconstruction and reactivity of pericytes and glial cells around the capillary endothelium occur in response to traumatic brain injury, epilepsy, and neurodegenerative disorders, impacting vascular permeability and participating in neuroinflammation. As this represents a growing field of research, addressing the multicellular reorganization occurring at the outer wall of the blood-brain barrier (BBB) in response to an acute insult or a chronic disease could disclose novel disease mechanisms and therapeutic targets.

Evolution of Mesoscale Convective System Organizational Structure and Convective Line Propagation

This study examines organizational changes and periods of rapid forward propagation in an MCS on 6 July 2015 in South Dakota. The MCS case was the focus of a Plains Elevated Convection at Night (PECAN) IOP. Data from the Sioux Falls WSR-88D and a high-resolution WRF simulation are analyzed to examine two periods of rapid forward propagation (or surges) and organizational changes. During the first surge (surge A), the northern portion of the convective line propagates eastward faster than the southern portion, and the northern portion of the leading line transitions from a single convective core to a multicellular structure as it merges with convection initiation. Radar reflectivity factor Z and graupel concentrations decrease above the melting layer, while at lower altitudes Z increases. The MCS cold pool also intensifies and deepens beneath an expanded region of high rainwater content and subsaturated air. Throughout surge A, a mesoscale circulation with strong rear-to-front near-surface flow and front-to-rear midlevel flow is also evident. By the end of surge A, the leading edge of the MCS cold pool is beneath developing convection initiation ahead of the original convective line while the original convective updraft weakened and moved rearward. This MCS evolution is similar to discrete propagation events discussed in past studies, except with new convection developing along an intersecting convective band. During surge B, the MCS transitions from a multicellular structure to a single, intense updraft. Smaller microphysical and thermodynamic changes are observed within the MCS during surge B compared to surge A, and the mesoscale circulation continues to develop.