Quantification of microbial robustness

Stable cell performance in a fluctuating environment is essential for sustainable bioproduction and synthetic cell functionality; however, microbial robustness is rarely quantified. Here, we describe a high-throughput strategy for quantifying robustness of multiple cellular functions and strains in a perturbation space. We evaluated quantifications theory on experimental data and concluded that the mean-normalized Fano factor allowed accurate, reliable, and standardized quantification. Our methodology applied to perturbations related to lignocellulosic bioethanol production showed that Saccharomyces cerevisiae Ethanol Red exhibited both higher and more robust growth rates than CEN.PK and PE-2, while a more robust product yield traded off for lower mean levels. The methodology validated that robustness is function-specific and characterized by positive and negative function-specific trade-offs. Systematic quantification of robustness to end-use perturbations will be important to analyze and construct robust strains with more predictable functions.

Introduction

What was sovereignty supposed to be, and what purpose might it serve for our common future? This study addresses these questions by examining the legal and political thought of Jean Bodin (1529/30–1596), widely regarded as the preeminent theorist of sovereignty in early modern political thought. This Introduction offers a preview of four principal themes and arguments to be explored in this book: (1) sovereignty as a permissive legal right; (2) Bodin’s concept of positive law as duty-creating command; (3) the negative function of absolute power; (4) the pluralistic structure of the Bodinian sovereign state. Special emphasis will be placed on Bodin’s outsized, though overlooked, influence on the intellectual origins of modern public international law, whose architecture is still anchored fundamentally in the notion of a state’s sovereign right.

Hilangnya Sifat Melawan Hukum Pidana Materil Dalam Tindak Pidana Korupsi Pasca Pengembalian Seluruh Kerugian Keuangan Negara

Criminal conduct can be held accountable when it meets two elements of such action as unlawful action and can be reproached. The nature of resisting the law itself is devided into two opposing qualities of formal law and the nature of resisting the law of materiel. The nature of the law regarding material itself has two fuction: itsnegative function and its positive work. Of these functions in application only to its negative (or exempted from the written law) function because of its positive function (an act not prohibited by the law but by society of the act is mistaken) and therefore is a violation of the law. This type of research is the normative study with the constitution approach (statue approach) and the conceptual approach. From this research it can be concluded that the repayment of all financial losses in the state can be excused/ fail d;excuse, so that the nature of resisting the laws of materiel’s negative function of the perpetrators of corruption is lost. The provision of article 4 of the constitution is judged as irrelevant because the application of a fixed sanction without seeing thr benefits of its ratification. This is based on the theory presented by Nigel Walker and Jeremmy Bentham, which says the application of criminal santions must be ideal and beneficial to the perpetrator. Keywords: Loss of Unlawful Character; Corruption; Recovery of State Losses.Perbuatan pidana dapat dimintai pertanggungjawaban apabila memenuhi dua unsur yakni perbuatan tersebut merupakan perbuatan melawan hukum dan perbuatan tersebut dapat dicela. Sifat melawan hukum itu sendiri terbagi dalam dua yakni sifat melawan hukum formal dan sifat melawan hukum materiel, Sifat melawan hukum materiel itu sendiri memiliki dua fungsi yaitu fungsinya yang negatif dan fungsinya yang positif. Dari kedua fungsi tersebut dalam penerapannya hanya diperbolehkan fungsinya yang negatif (atau perbuatan yang dapat dikecualikan dari hukum yang tidak tertulis) dikarenakan fungsinya yang positif (perbuatan yang tidak dilarang oleh undang-undang tetapi oleh masyarakat perbuatan tersebut dianggap keliru) sehingga fungsinya ini dianggap bertentangan dengan undang-undang. Jenis Penelitian ini ialah penelitian normatif dengan pendekatan undang-undang (Statute Approach) dan pendekatan konseptual (Conceptual Approach). Dari penelitian ini dapat disimpulkan bahwa dengan dikembalikannya seluruh kerugian keuangan negara maka perbuatan pelaku dapat dimaafkan/fail d;excuse, sehingga sifat melawan hukum materiel fungsinya yang negatif dari perbuatan pelaku tindak pidana korupsi hilang. Sehingga ketentuan dalam Pasal 4 UU dinilai sudah tidak relevan dikarenakan penerapan sanksi yang tetap dijatuhkan tanpa melihat manfaat dari penjatuhan sanksi tersebut. Hal tersebut berdasarkan pada teori yang dikemukakan oleh Nigel Walker dan Jeremmy Bentham, yang mengatakan bahwa penerapan sanksi pidana harus ideal dan bermanfaat bagi pelaku.Kata Kunci: Hilangnya Sifat Melawan Hukum; Korupsi; Pengembalian Kerugian Negara.

Multipliers and closures of Besov-type spaces in the Bloch space

Let p > 1 and let ? be a non-negative function defined in R+. A function f ? H(D) belongs to the space Bp(?) (see [4]) if ||f||p Bp(?) = |f(0)jp + ?D |(1-|z|2) f?(z)|p ?(1-|z|2)/(1-|z|2)2 dA(z) < ?. In this paper, motivated by the works of B?koll? and Bao and G???s, under some conditions on the weight function ?, we investigate the closures CB(B ? Bp(?)) of the spaces B ? Bp(?) in the Bloch space. Moreover we prove that interpolating Blaschke products in CB(B ? Bp(?)) are multipliers of Bp(?) ? BMOA.

On robust weakly $ \varepsilon $-efficient solutions for multi-objective fractional programming problems under data uncertainty

<abstract><p>In this study, we use the robust optimization techniques to consider a class of multi-objective fractional programming problems in the presence of uncertain data in both of the objective function and the constraint functions. The components of the objective function vector are reported as ratios involving a convex non-negative function and a concave positive function. In addition, on applying a parametric approach, we establish $ \varepsilon $-optimality conditions for robust weakly $ \varepsilon $-efficient solution. Furthermore, we present some theorems to obtain a robust $ \varepsilon $-saddle point for uncertain multi-objective fractional problem.</p></abstract>

Cognitive science: from paradigms to theoretical complexes

This article deals with the task of understanding main theoretical movements in cognitive science, including classical computational cognitivism, connectionism, moderate embodied cognition, and predictive processing. For this purpose, the article analyzes the well-known post-positivist conceptions of philosophy of science developed by T. Kuhn, I. Lakatos, and L. Laudan, which focus not on individual theories, but on groups of theories. Despite the fact that all these well-known post-positivist conceptions describe well certain features of theoretical movements in cognitive science, none of them as such can be taken as a basis for understanding those cognitivist groups of theories and models. Thus, the article develops an alternative approach based on the author’s idea of theoretical complexes. With the help of this idea, it becomes possible, firstly, to characterize the form of organization of main theoretical movements in cognitive science. From this point of view, complexes of individual theories, models, and conceptions in cognitive science can be formed both on the basis of one common property or a number of common properties, and on the basis of family resemblance. And, secondly, the idea of theoretical complexes has made it possible to clarify the basic functions of cognitivist theoretical movements. These functions include the constructive function of a landmark for the supporters of one particular complex (including the subordinate functions of creating and modifying individual theories, defining their basic concepts, etc.). and the negative function of a target for criticism for supporters of competing complexes.

Boundary value problems associated with singular strongly nonlinear equations with functional terms

We study boundary value problems associated with singular, strongly nonlinear differential equations with functional terms of type $$\big({\it \Phi}(k(t)\,x'(t))\big)' + f(t,{{\mathcal{G}}}_x(t))\,\rho(t, x'(t)) = 0,$$ on a compact interval [a, b]. These equations are quite general due to the presence of a strictly increasing homeomorphism Φ, the so-called Φ-Laplace operator, of a non-negative function k, which may vanish on a set of null measure, and moreover of a functional term Gx. We look for solutions, in a suitable weak sense, which belong to the Sobolev space W1,1([a, b]). Under the assumptions of the existence of a well-ordered pair of upper and lower solutions and of a suitable Nagumo-type growth condition, we prove an existence result by means of fixed point arguments.

Fluorescent tagging of Plasmodium circumsporozoite protein allows imaging of sporozoite formation but blocks egress from oocysts

The circumsporozoite protein, CSP is the major surface protein of Plasmodium sporozoites, the form of malaria parasites transmitted by mosquitoes. CSP is involved in sporozoite formation within and egress from oocysts, entry into mosquito salivary glands and mammalian liver as well as migration in the skin. Antibodies against CSP can stop infection prior to the first round of parasite replication in the liver. CSP consists of different domains and is proteolytically cleaved prior to hepatocyte invasion. Part of CSP has been developed into a licensed vaccine against malaria. Yet, how CSP facilitates sporozoite formation, oocyst egress and hepatocyte specific invasion is still not fully understood. Here, we generated a series of parasites expressing full-length versions of CSP as fusion proteins with the green fluorescent protein. This enabled the investigation of sporozoite formation in living oocysts and revealed a dominant negative function of some GFP-CSP fusions during sporozoite egress.

Seed size, number and strategies in annual plants: a comparative functional analysis and synthesis

Background and Aims Plants depend fundamentally on establishment from seed. However, protocols in trait-based ecology currently estimate seed size but not seed number. This can be rectified. For annuals, seed number should simply be a positive function of vegetative biomass and a negative function of seed size. Methods Using published values of comparative seed number as the ‘gold standard’ and a large functional database, comparative seed yield and number per plant and per m2 were predicted by multiple regression. Subsequently, ecological variation in each was explored for English and Spanish habitats, newly calculated C-S-R strategies and changed abundance in the British flora. Key Results As predicted, comparative seed mass yield per plant was consistently a positive function of plant size and competitive ability, and largely independent of seed size. Regressions estimating comparative seed number included, additionally, seed size as a negative function. Relationships differed numerically between regions, habitats and C-S-R strategies. Moreover, some species differed in life history over their geographical range. Comparative seed yield per m2 was positively correlated with FAO crop yield, and increasing British annuals produced numerous seeds. Nevertheless, predicted values must be viewed as comparative rather than absolute: they varied according to the ‘gold standard’ predictor used. Moreover, regressions estimating comparative seed yield per m2 achieved low precision. Conclusions For the first time, estimates of comparative seed yield and number for &gt;800 annuals and their predictor equations have been produced and the ecological importance of these regenerative traits has been illustrated. ‘Regenerative trait-based ecology’ remains in its infancy, with work needed on determinate vs. indeterminate flowering (‘bet-hedging’), C-S-R methodologies, phylogeny, comparative seed yield per m2 and changing life history. Nevertheless, this has been a positive start and readers are invited to use estimates for &gt;800 annuals, in the Supplementary data, to help advance ‘regenerative trait-based ecology’ to the next level.

On mutually inverse transforms of functions on a half-line

Two transforms of functions on a half-line are considered. It is proved that their composition gives a concave majorant for every non-negative function. In particular, this composition is an identical transform on the class of non-negative concave functions. Applications of this result in the operator theory of Hilbert space and in the theory of quantum systems are mentioned. Several open problems are formulated.