Livestock, pathogens, vectors, and their environment: a causal inference-based approach to estimating the pathway-specific effect of livestock on human African trypanosomiasis risk

Livestock are important reservoirs for many diseases, and investigation of such zoonoses has long been the focus of One Health research. However, the effects of livestock on human and environmental health extend well beyond direct disease transmission. In this retrospective ecological cohort study we use pre-existing data and methods derived from causal inference and spatial epidemiology to estimate three hypothesized mechanisms by which livestock can come to bear on human African trypanosomiasis (HAT) risk: the reservoir effect, by which infected cattle and pigs are a source of infection to humans; the zooprophylactic effect, by which preference for livestock hosts exhibited by the tsetse fly vector of HAT means that their presence protects humans from infection; and the environmental change effect, by which livestock keeping activities modify the environment in such a way that habitat suitability for tsetse flies, and in turn human infection risk, is reduced. We conducted this study in four high burden countries: at the point level in Uganda, Malawi, and Democratic Republic of Congo (DRC), and at the county-level in South Sudan. Our results indicate cattle and pigs play an important reservoir role for the rhodesiense form (rHAT) in Uganda, however zooprophylaxis outweighs this effect for rHAT in Malawi. For the gambiense form (gHAT) we found evidence that pigs may be a competent reservoir, however dominance of the reservoir versus zooprophylactic pathway for cattle varied across countries. We did not find compelling evidence of an environmental change effect.

Bioinspired, electroactive colorable and additive manufactured photonic artificial muscle

Structural coloration in biomimetic nanostructures has remarkable application potentials in vivid display devices, but its color change effect is still insufficiently competitive towards biology. Inspired by the feather color change...

Dimension investigation to pneumatic network bending soft actuators for soft robotic applications

Soft actuators have been investigated for robots that interact with people, such as industrial robots, entertainment robots, and medical robots. Although soft actuators have been utilised in several applications, design tools that can assist in the effective and systematic design of actuators are needed. This paper focused on the most common soft actuators for bending motion, the Pneumatic Networks bending actuators. Paper presented a survey on the effects of changing the dimensions on the soft actuator and its cross-section shape on the soft actuator flexibility and the forces generated at different applied pressures. This survey can be used to optimize the dimension ratio for the soft actuator and the cross-section shape. Furthermore, this paper analyzed the possible reasons for the dimension change effect. The performance of the bending soft actuator was evaluated using ABAQUS/CAE software simulation models to provide quantitative insights into the actuators' behaviours. Thus, this paper provided a lot of insights that can be used to guide and accelerate the soft actuator design process to create strong and flexible Pneumatic Networks bending actuators. Using the paper insights outputs, a soft gripper was designed that can grasp many complex objects without needing any modification in the gripper shape. To show the proposed actuators' capacity to do complicated movements and expand their applications, a completely soft hand was created that can mimic the mobility of the human hand as nearly as possible, and this ability was verified using hand sign language settings.

Study of climate change effect on water balance in upper Citarum Watershed, the Krueng Cunda Watershed, and the Woske Watershed, Indonesia

The community feels the changes in water availability due to climate change will directly impact water availability, especially water availability in rivers, reservoirs and other water reservoirs. The purpose of this analysis is to determine changes in the rainy season shift, changes in mainstay discharge, and the effect of climate change on water balance. The results show a shift in the rainy season and a significant decrease in rainfall during the last ten years. In the Upper Citarum watershed (UCW), there is a change in the maximum and minimum mainstay discharge in the three watersheds. Climate change significantly affects the water balance in a watershed. Meanwhile, in the Krueng Cunda watershed (KCW) and the Woske watersheds (WW), there was a decrease in the value of the water balance, which was the same as the UCW. However, still able to meet the water needs of the population. There was a decrease in the value of the water balance, which was the same as the UCW, but still able to meet the population’s water needs.

Random clonal expansion as a limiting factor in transplantable in vivo CRISPR/Cas9 screens

Transplantable in vivo CRISPR/Cas9 knockout screens, in which cells are transduced in vitro and inoculated into mice to form tumours in vivo, offer the opportunity to evaluate gene function in a cancer model that incorporates the multicellular interactions of the tumour microenvironment. In this study, we sought to develop a head and neck squamous cell carcinoma (HNSCC) tumour xenograft model for whole-genome screens that could maintain high gRNA representation during tumour initiation and progression. To achieve this, we sought early-passage HNSCC cell lines with a high frequency of tumour initiation-cells, and identified the pseudodiploid UT-SCC-54C line as a suitable model from 23 HNSCC lines tested based on a low tumourigenic dose for 50% takes (TD50) of 1100 cells in NSG mice. On transduction with the GeCKOv2 whole-genome gRNA library (119,461 unique gRNAs), high (80-95%) gRNA representation was maintained in early (up to 14 d) UT-SCC-54C tumours in NSG mice, but not in UT-SCC-74B tumours (TD50=9200). However, loss of gRNA representation was observed in UT-SCC-54C tumours following growth for 38-43 days, which correlated with a large increase in bias among gRNA read counts due to stochastic expansion of clones in the tumours. Applying binomial thinning simulations revealed that the UT-SCC-54C model would have 40-90% statistical power to detect drug sensitivity genes with log2 fold change effect sizes of 1-2 in early tumours with gRNA libraries of up to 10,000 gRNAs and modest group sizes of 5 tumours. In large tumours, this model would have had 45% power to detect log2 fold change effect sizes of 2-3 with libraries of 2,000 gRNAs and 14 tumours per group. Based on our findings, we conclude that gRNA library size, sample size and tumour size are all parameters that can be individually optimised to ensure transplantable in vivo CRISPR screens can successfully evaluate gene function.

Smart Village: A new dynamic to end rural urban gap and move towards sustainable development for all

Smart rural development is one of the best criteria to achieve Sustainable Development, there are 60,000 villages in India, where the condition of some of the villages are extremely miserable with no access to water, food and employment. With advancement in technology and bringing in green technology India has made few of its villages developed and constructed them as smart village with 100 % energy security, water access, pucca house, internet connectivity, empowering women, installation of RO, better jobs and government schools which have introduced computer learning. With this smart village development it has reduced the migration rate and brough back the people who went to the urban sector in such of better standard of living and employment. Smart village is a private public partnership model. Smart rural village will help to eradicate poverty, hunger, educated everyone, smart villages have seen an up gradation in the number of students. With sustainable development and smart techniques, urban overpopulation problem can be reduced and urbans slums region will also seen a decline as majority of the people migrating from rural to urban live in the urban slums, will help to reduce the climate change effect by green technology and smart living.

Quantitative Study of Photoelectric Laser Stimulation for Logic State Imaging in Embedded SRAM

The use of optical techniques for attacking integrated circuits (ICs) at the silicon level is increasingly being reported. Although these attacks can be complex to set and require skilled attacker that can access expensive equipment, they are nonetheless very powerful. Among the different applications described in literature, there has been a focus on extracting data directly from embedded SRAM. Such attacks can provide access to highly sensitive information such as encryption keys and bypass various security strategies. An attacker usually exploits one of the several interactions that exist between light and semiconductor to generate an image where content can be directly qualified by the data in memory (Logic State Image – LSI). Thermal laser stimulation (TLS) and laser probing (EOFM-Electro-Optical Frequency Mapping) have been reported in the literature recently but Photoelectric Laser Stimulation (PLS) did not get as much attention. Considering the potential advantages of PLS over other techniques (e.g. lower power requirements to generate current/voltage change, effect can be triggered at shorter wavelength which may lead to an improved spatial resolution), we investigate in this paper if logic state images can be generated with PLS on a variety of devices and do a comparative assessment with state-of-the-art technologies to assess potential benefits and limitations.

Change of Bandgap Energy in Quantum System of Nanolayer on Silicon

In the quantum system of nanolayer (NL) on silicon, the bandgap energy obviously increases with the decrease of NL thickness, where the quantum confinement (QC) effect plays the main role as the thickness of Si NL changes along with (100), (110) and (111) directions, respectively. And the simulation result demonstrated that the direct bandgap can be obtained as the NL with (001) direction is thinner than 10 nm on Si surface. However, it is discovered in the simulated calculation that the QC effect disappears as the NL thickness arrives at the size of the monoatomic layer, in which its bandgap sharply decreases, where the abrupt change effect in bandgap energy occurs near-ideal 2D-layer. In the experiment, we fabricated the Si NL structure by using electron beam irradiation and laser deposition methods, in which a novel way was used to control the NL thickness by modulating irradiation time of the electron beam. The new effect should have a good application on a photonic-electronic chip of silicon.

Climate Change Effect on Irrigation Water Requirement of Wheat and Maize in Northern Part of Bangladesh

Bangladesh is one of the most vulnerable countries for climate change in agricultural water management. A research had been done to assess climate change effects on irrigation water use of wheat and maize in the northern part of Bangladesh. The twenty nine years of data (1990-2018) were analyzed with Mann-Kendall test as well as Sen’s slope for climate change impact and the responsible weather parameters due to climate change were identified with correlation coefficients. The crop water requirement of wheat in Bogura and Rangpur was declining at the rate of 3.3mm and 2.3mm per decade respectively. Net irrigation water requirement of wheat at both Bogura and Rangpur was inclining at the rate of 1mm and 10mm per decade respectively because the effective rainfall of these regions was decreasing at 5mm and 11mm per decade respectively. The crop water requirement of maize for similar districts was increasing at the rate of 3.2mm and 2.5mm per decade respectively although net irrigation water requirement had statistically non-significance for climate change effect. The weather parameter, which was mainly responsible for climatic change in irrigation water requirement, was increasing temperature. Therefore, wheat cultivation might be coped with climate change in the northern part of Bangladesh rather than maize on the basis of irrigation and water management.