Response Calls Evoked by Playback of Natural 50-kHz Ultrasonic Vocalizations in Rats

Rats are highly social animals known to communicate with ultrasonic vocalizations (USV) of different frequencies. Calls around 50 kHz are thought to represent a positive affective state, whereas calls around 22 kHz are believed to serve as alarm or distress calls. During playback of natural 50-kHz USV, rats show a reliable and strong social approach response toward the sound source. While this response has been studied in great detail in numerous publications, little is known about the emission of USV in response to natural 50-kHz USV playback. To close this gap, we capitalized on three data sets previously obtained and analyzed USV evoked by natural 50-kHz USV playback in male juvenile rats. We compared different rat stocks, namely Wistar (WI) and Sprague-Dawley (SD) and investigated the pharmacological treatment with the dopaminergic D2 receptor antagonist haloperidol. These response calls were found to vary broadly inter-individually in numbers, mean peak frequencies, durations and frequency modulations. Despite the large variability, the results showed no major differences between experimental conditions regarding call likelihood or call parameters, representing a robust phenomenon. However, most response calls had clearly lower frequencies and were longer than typical 50-kHz calls, i.e., around 30 kHz and lasting generally around 0.3 s. These calls resemble aversive 22-kHz USV of adult rats but were of higher frequencies and shorter durations. Moreover, blockade of dopamine D2 receptors did not substantially affect the emission of response calls suggesting that they are not dependent on the D2 receptor function. Taken together, this study provides a detailed analysis of response calls toward playback of 50-kHz USV in juvenile WI and SD rats. This includes calls representing 50-kHz USV, but mostly calls with lower frequencies that are not clearly categorizable within the so far known two main groups of USV in adult rats. We discuss the possible functions of these response calls addressing their communicative functions like contact or appeasing calls, and whether they may reflect a state of frustration. In future studies, response calls might also serve as a new read-out in rat models for neuropsychiatric disorders, where acoustic communication is impaired, such as autism spectrum disorder.

Associations between Dairy Herds’ Qualitative Behavior and Aspects of Herd Health, Stockperson and Farm Factors—A Cross-Sectional Exploration

The affective state is an integrated aspect of farm animal welfare, which is understood as the animals’ perception of their living environment and of their internal biological functioning. The aim of this cross-sectional study was to explore animal-internal and external factors potentially influencing dairy cows’ affective state. For this purpose, qualitative behavior assessments (QBA) describing the animals’ body language were applied at herd level on 25 dairy farms. By means of principal component analysis (PCA), scores of PC1 (QBAscores) were determined for further analyses. From monthly milk recordings (MR) one year retrospectively, prevalences of udder and metabolic health impairments were calculated. Factors of housing, management, and human-animal contact were recorded via interviews and observations. A multivariable regression was calculated following a univariable preselection of factors. No associations were found between MR indicators and QBAscores. However, more positive QBAscores were associated with bedded cubicles or straw yards compared to raised cubicles, increased voluntary stockperson contact with the cows, and fixation of cows during main feeding times, the latter contributing to the explanatory model, but not being significant. These results underline the importance of lying comfort, positive human-animal relationship and reduction of competition during feeding for the well-being of dairy cows.

The use of ball pits and playpens in laboratory Lister Hooded male rats induces ultrasonic vocalisations indicating a more positive affective state and can reduce the welfare impacts of aversive procedures

The advancement and quality of science rely on research that is robust and unbiased in its experimental design, execution, analysis, and reproducibility. In preclinical research, a better understanding of animal emotions and refinement of their husbandry, housing, and handling are important goals in providing good animal welfare in a laboratory setting which underpins rigorous research quality. Induction of positive emotional state in animals is a key component of their well-being, and one approach is to increase their environmental complexity using, for example, ball pits or playpens in rats. In this study, we recorded 50 kHz ultrasonic vocalisations (USVs) during animals’ exposure to the ball pit and playpen. We have previously shown that 50 kHz USVs provide a graded and quantifiable measure of an animal’s emotional state, and here find that access to the ball pit and playpen increases 50 kHz USVs, indicative of a more positive affective state. Using our affective bias test (ABT) we next quantified the animals’ emotional response to an aversive intervention and whether this could be attenuated by access to a playpen. The playpen exposure completely mitigated the negative affective state induced by an anxiogenic drug when compared with animals who experienced the drug in the home cage. Together, these findings suggest ball pits and playpens provide a simple and effective method to improve the welfare of laboratory rats and reduce the cumulative suffering they experience from their housing conditions and minor, aversive procedures.

The Meaning of Home in Male Migration: Listening to Men’s Experiences

We assessed the sense of psychological home among adult men (n = 17; M age = 29.7 years old) who had experienced migration to Italy, focusing on the relationship between psychological home and the process of integration into the new country. Psychological home is a dynamic process in which people sense a safe and secure environment that ranges beyond the confines of a structured dwelling, a process which is reflective and which communicates one’s self-identity. Participants engaged in a semistructured interview with the aim of establishing a generic concept of psychological home and identifying the issues that arise at the intersection of psychological home and migration. The results highlighted certain themes about the meaning that psychological home assumes in the lives of migrants and about the way in which the migration experience acts to support or hinder the process of building this sense of home. Of special interest is the idea that individuals might develop multiple psychological homes related to the different places and relationships that they experience. In this sense, establishment of a psychological home might be considered the ideal affective state for psychological adaptation to a new country.

No evidence for trait- and state-level urgency moderating the daily association between negative affect and subsequent alcohol use in two college samples

It remains unclear whether the negative reinforcement pathway to problematic drinking exists, and if so, for whom. One idea that has received some support recently is that people who tend to act impulsively in response to negative emotions (i.e., people high in negative urgency) may specifically respond to negative affect with increased alcohol consumption. We tested this idea in a preregistered secondary data analysis of two ecological momentary assessment studies using college samples. Participants (N = 226) reported on their current affective state multiple times per day and the following morning reported alcohol use the previous night. We assessed urgency both at baseline and during the momentary affect assessments. Results from our Bayesian model comparison procedure, which penalizes increasing model complexity, indicate that no combination of the variables of interest (negative affect, urgency, and the respective interactions) outperformed a baseline model that included two known demographic predictors of alcohol use. A non- preregistered exploratory analysis provided some evidence for the effect of daily positive affect, positive urgency, as well as their interaction on subsequent alcohol use. Taken together, our results suggest that college students’ drinking may be better described by a positive rather than negative reinforcement cycle.

Beliefs in Misinformation and Conspiracy Theories About COVID- 19: a Triangular Relationship Between Analytic Thinking, Depressive States, and Misperception

Beliefs about misinformation and conspiracy theories are often associated with a state of mind. With the spread of the pandemic there has been an outbreak of depression cases among the population. In this paper, we attempt to test the relationship between affective states and beliefs in misinformation about COVID-19 during the early phase of the pandemic. We do this through a survey carried out on a random and representative sample of the Italian population that allows us to go and verify the co-evolution of many factors: i.e., beliefs in misinformation, symptoms of depression, perceptions and misperceptions about COVID-19, ways in which citizens got informed about the pandemic, and sociodemographic characteristics (e.g., age, gender, education). The results show that the relationship between affective state and beliefs in misinformation exists but is more complex than hypothesized.

THE EFFECTS OF CREATIVE MEDITATION ON PSYCHOLOGICAL DISTRESS AND AWARENESS CAPACITY DURING COVID 19 PANDEMIC

Creative meditation is an effective, innovative and safe psychotherapeutic intervention that may improve psychological health and well-being during the Covid 19 pandemic. Creative meditation creates a safe place for self-awareness and self-expression, allowing the discovery and development of personal resources, converting vulnerabilities into resources and facilitating personal development. The aim of the present study is to investigate the immediate effect of creative meditation on emotional distress and awareness capacity (acceptance and mindfulness). 22 students participated in a short experiment of a single online creative meditation session with pre and post surveys. An immediate significant variation in the participants’ emotional experience was observed. Creative meditation helped participants to feel a positive affective state which facilitated awareness and creative emotional and cognitive expression. Statistical results revealed a decrease of general affective distress, and a lower level of negative emotions (sadness, anxiety, frightened, concerned). The personal resources could be first actualized through this emotional experience and then it could be invested into meaningful actions.

The Operational Process of Nostalgia: The Trigger Determines the Nostalgic Experience

Nostalgia is a complex affective state with a strong cognitive component focussed on past autobiographical memories. This complex nature of nostalgia is discussed in the literature but there are scarce empirical observations of state nostalgic processes. In this study, the operational process of nostalgia is explored by the use of two types of stimuli—indirect (instructions) and direct (music)—with a sample of 285 participants (18–35 years old) in a mixed-method experimental design. Nostalgia was observed in terms of its operational process (reaction time, duration, intensity and variation), the characteristics of the recalled event (event type and contents), and the nature of the nostalgic experience (its phenomenology, affect and motivation). The studied parameters of all three nostalgic components were found to vary with the stimulus that triggered it. The study shows that nostalgia is not a constant phenomenon but depends on the conditions that give rise to it.

The dot-probe attention bias task as a method to assess psychological wellbeing after anesthesia: A study with adult female long-tailed macaques (Macaca fascicularis)

Understanding the impact routine research and laboratory procedures have on animals is crucial to improving their wellbeing and to the success and reproducibility of the research they are involved in. Cognitive measures of welfare offer insight into animals’ internal psychological state, but require validation. Attention bias - the tendency to attend to one type of information over another – is a cognitive phenomenon documented in humans and animals that is known to be modulated by affective state (i.e., emotions). Hence, changes in attention bias may offer researchers a deeper perspective of their animals’ psychological wellbeing. The dot-probe task is an established method for quantifying attention bias in humans (by measuring reaction time to a dot-probe replacing pairs of stimuli), but has yet to be validated in animals. We developed a dot-probe task for long-tailed macaques (Macaca fascicularis) to determine if the task can detect changes in attention bias following anesthesia, a context known to modulate attention and trigger physiological arousal in macaques. Our task included the following features: stimulus pairs of threatening and neutral facial expressions of conspecifics and their scrambled counterparts, two stimuli durations (100 and 1000 ms), and counterbalancing of the dot-probe’s position on the touchscreen (left, right) and location relative to the threatening stimulus. We tested eight group-housed adult females on different days relative to being anesthetized (baseline and one-, three-, seven-, and 14-days after). At baseline, monkeys were vigilant to threatening content when stimulus pairs were presented for 100 ms, but not 1000 ms. On the day immediately following anesthesia, we found evidence that attention bias changed to an avoidance of threatening content. Attention bias returned to threat vigilance by the third day post-anesthesia and remained so up to the last day of testing (14 days after anesthesia). We also found that attention bias was independent of the type of stimuli pair (i.e., whole face vs. scrambled counterparts), suggesting that the scrambled stimuli retained aspects of the original stimuli. Nevertheless, whole faces were more salient to the monkeys as responses to these trials were generally slower than to scrambled stimulus pairs. Overall, our study suggests it is feasible to detect changes in attention bias following anesthesia using the dot-probe task in non-human primates. Our results also reveal important aspects of stimulus preparation and experimental design.

Malaysian School Counselor’s Self-Efficacy: The Key Roles of Supervisor Support for Training, Mastery Experience, and Access to Training

The concept of self-efficacy has been widely studied and shown to contribute to individuals’ job satisfaction. For counselors, the concept measures their belief in their ability to conduct counseling sessions. However, it is an understudied area. As Bandura states, self-efficacy and its sources should be investigated and measured within its domain, which in this case is school counseling. This study examined the impact on school counselors’ self-efficacy and job satisfaction of the personal and environmental factors: (a) mastery experience, (b) social persuasion, (c) vicarious learning, (d) physiological and affective state, (e) the access to training, and (f) perceived supervisor support of training. The cross-sectional study involved 541 Malaysian secondary school counselors nationwide via a random sampling-distributed questionnaire. Results which were analyzed using PLS-SEM, with importance-performance functionality embedded in it, indicated that mastery experience, access to training, and perceived supervisor support of training explained 45.6% variance in counseling self-efficacy and together with counseling self-efficacy, contributed 13.2% variance in job satisfaction among the school counselors. The importance-performance map analysis revealed supervisor support of training as of greatest importance in shaping counseling self-efficacy. Counseling self-efficacy partially mediated the relationship between mastery experience, access to training, supervisor support toward training, and job satisfaction Arising from this finding is a proposed theoretical framework in which efficacy information (i.e., mastery experience), environmental determinants (i.e., access to training and supervisor support of training) and cognitive determinant (i.e., counseling self-efficacy) corresponded together congruently and lead to higher job satisfaction. Suggestions are also made for training providers, content developers, and policymakers to include these factors in professional development training and continuous education, to sustain the wellbeing of school counselors.