Association of the AGTR1 Gene A1166C (rs5186) Polymorphism with Essential Hypertension in the Indigenous Population of the Arctic

The research objective was to study the association of the AGTR1 rs5186 SNP (the A1166C variant) with essential hypertension among indigenous people of the Arctic territory of Yakutia. Methods and Results: A total of 351 participants (224 women and 127 men) were examined, including 56 Yakuts, 34 Chukchi, 77 Yukaghirs, and 184 Evens. The Case (n=168) and Control (n=183) groups were formed. Allelic variants of the AGTR1 rs5186 SNP were tested by real-time PCR. We did not find statistically significant differences in the frequency distribution of the alleles and genotypes of the AGTR1 rs5186 SNP between the Case group and the Control group. Conclusion: The obtained data show no association of the AGTR1 A1166C polymorphism with EHT in the representatives of indigenous people of the Arctic territory of Yakutia.

Risk factors and AGTR1 gene polymorphism (1166A>C) in patients with essential hypertension

Objective – to analyze the association of risk factors with the 1666 A>C polymorphism of the AGTR1 gene in patients with essential hypertension.Material and methods. 100 patients were screened, 72 of whom were genotyped. The control group consisted of 48 healthy individuals who did not differ in gender and age, and with the group of patients.Results. The obtained data confirmed that the level of blood pressure elevation is associated, to some extent, with modified (diabetes mellitus 2, smoking, body mass index) and unmodified factors (family history, gender) the risk of essential hypertension. The results of the analysis of blood pressure levels considering the A1166C polymorphism of the AGTR1 gene showed that the values of systolic and diastolic blood pressure in the group of patients with C-allele carriers were higher than in carriers of AA genotype: SBP – by 5.38% (p<0.05), DBP – by 5.15% (p<0.05). Conclusions. The level of blood pressure in patients with essential hypertension depends on body mass index and smoking. In carriers of the C-allele of the AGTR1 gene (A1166C), the level of systolic and diastolic blood pressure exceeds the ones of the carriers of the AA genotype. The presence of the C-allele of the AGTR1 gene (A1166C) almost doubles the risk of severe essential hypertension [OR = 2.75; p = 0.037].

Three polymorphisms of renin-angiotensin system and preeclampsia risk

Purpose Some data suggest an association between the single nucleotide polymorphisms AGT T704C, ACE I/D, and AT1R A1166C and preeclampsia, but overall, the data are conflicting; the aim of our study was to discover a more stable and reliable association between these polymorphisms and PE risk. Methods A comprehensive literature search for this meta-analysis was conducted. Odds ratios (OR) and 95% confidence intervals (CIs) were calculated to evaluate the strength, and heterogeneity test was conducted. Trial sequential analysis was also performed. Results A total of forty studies were finally included in our meta-analysis. The AGT T704C polymorphism was associated with PE risk in three genetic models (dominant OR = 1.33, 95%CI = 1.12–1.59; heterozygote OR = 1.26, 95%CI = 1.05–1.52; homozygote OR = 1.44, 95%CI = 1.14–1.83). No heterogeneity was observed in the three genetic models for the ACE I/D polymorphism. For subgroup analysis by geography, no significant association was detected. Significant associations were observed in mixed race, early-onset, late-onset, and more than 200 subgroups for the AT1R A1166C polymorphism; however, only one study was analyzed in these subgroups. Conclusions Our results indicated the AGT T704C and ACE I/D polymorphisms were associated with an increased risk of PE. Increased risks were also observed for the two polymorphisms in subgroups including Asians, Europeans, Caucasoid, and Mongoloid. Moreover, an increased PE risk with the ACE I/D polymorphism in the severe PE population was also detected. Regarding the AT1R A1166C polymorphism, weak associations were observed, but further studies are required.

Genetic polymorphism of renin-angiotensin-aldosterone system in type 2 diabetes and in combination with arterial hypertension among Dagestan inhabitants

BACKGROUND: Type 2 diabetes and arterial hypertension are frequent comorbidities under which activation the renin-angiotensin-aldosterone system is important pathogenetic link. The functional state of the RAAS is genetically determined. Genetic polymorphisms of the RAAS system associated with the development of both type 2 diabetes and arterial hypertension have been identified and mapped. Associations of polymorphic variants of the RAAS genes with type 2 diabetes and arterial hypertension among the inhabitants of Dagestan have not been studied. AIM: Studying the association of the most relevant polymorphic variants of the C521T and T704C AGT gene, as well as the A1166C AGTR1 gene with type 2 diabetes and when combining type 2 diabetes with arterial hypertension among Dagestan inhabitants. METHODS: We examined 16 patients with type 2 diabetes, 59 patients with type 2 diabetes combined with arterial hypertension and 51 patients with arterial hypertension, all residents of Dagestan. The control group included 47 healthy persons of the same age group. SNP polymorphisms were investigated by the method of allele-specific Real-Time PCR. The C521T and T704C polymorphisms of the AGT gene and the A1166C polymorphism of the AGTR1 gene were studied. RESULTS: In the group of patients with a combination type 2 diabetes with arterial hypertension, the genotype CT of the C521T polymorphism of the AGT gene is less common compared to the control (23% vs. 43%, 2 = 3,868, p = 0,049), OR score 0,4 (0,2-0,9 ). The situation is similar with the TC genotype of the T704C polymorphism of the AGT gene (39% versus 61%, 2 = 4,282, p = 0,039). OR was 0,4 (0,20,8).On the contrary, in the same patients, but the carriers of the homozygous CC genotype of the T704C polymorphism of the AGT gene, OR exceeded one and made 2.5 (1.02-5.9), the frequency of occurrence was 42% vs. 23%, 2 = 3,363, p = 0,05. The frequency of the mutant allele C of the A1166C polymorphism of the AGTR1 gene in patients with arterial hypertension alone was 31% vs. 14%, 2 = 5.496, p = 0,019, OR 2,5 (1,2-5,0). The frequency of the wild allele A in these same patients was 69% versus 84%, 2 = 5,496, p = 0,019, OR 0,4 (0,2-0,8). A similar situation is determined with the AA genotype (52% versus 73%, 2 = 3,609, p = 0,05), OR = 0,4 (0,1-0,9). CONCLUSIONS: The association of the C521T and T704C polymorphisms, as well as the A1166C candidate genes AGT and AGTR1 with type 2 diabetes and arterial hypertension, is an important component in assessing the susceptibility to the development of these diseases in Dagestan residents.

GENETIC ASPECTS OF PREGNANCY MISCARRIAGE

Pregnancy miscarriage is a consequence of many factors. The aim of the study was to analyze the effect of miscarriage gene on embryometric, ultrasound, hormonal, immunological parameters in pregnant women, and to evaluate its prognostic value. The main group includes 31 pregnant women who had clinical signs of miscarriage in current or previous pregnancy. The control group consists of 32 healthy pregnant women whose clinical-paraclinical parameters served as a control to compare the data of the pregnancy survey of the main surveillance group. A general clinical examination and a special obstetrical examination (complaints, anamnesis, general medical examination, obstetric examination), biochemical studies (determination of hormones of the fetoplacental complex in blood serum of pregnant women), ultrasound, immunological studies, histological studies of the placenta, molecular genetic study A1166C polymorphism of the AGTR1 gene were made. In the course of the research, the genetic determinism of miscarriage was discovered. The polymorphism of the A1166C of the AGTR1 gene was considered as a prognostic marker of miscarriage in early gestational term and preeclampsia in the second half of pregnancy. A reliable marker of abortion was the maternal genotype 1166AC for the genome AGTR1. The risk of occurrence of clinical manifestations of abortion increased five times. At simultaneous influence of all prognostic factors the risk of abortion increased 6,25 times. Detection of genetic markers of pregnancy miscarriage will allow early correction of this pathology and prevent perinatal loss.

Association of angiotensin ІІ type 1 receptor gene A1166C polymorphism with cancer risk: An updated meta-analysis

Objective: The association between angiotensin II type 1 receptor ( AGTR1) gene A1166C polymorphism and cancer risk has been investigated in many studies. However, the results have been inconclusive. A meta-analysis was performed to obtain a more precise estimation of the relationship. Methods: The PubMed and China National Knowledge Infrastructure databases were searched for published literature. Odds ratios (ORs) with 95% confidence intervals (CIs) were used to assess the strengths of association. Results: Ten studies, including 1553 patients and 1904 controls, were included in the meta-analysis. Overall, there were no significant associations between the AGTR1 gene A1166C polymorphism and cancer risk in the general population (CC vs AA: OR = 1.09, 95% CI = 0.50–2.37; AC vs AA: OR = 1.54, 95% CI = 0.81–2.91; dominant model: OR = 1.46, 95% CI = 0.77–2.79; recessive model: OR = 1.12, 95% CI = 0.84–1.49). In a subgroup analysis by nationality and cancer type, the results also showed no association between this polymorphism and cancer risk. Conclusions: This meta-analysis demonstrated that the AGTR1 gene A1166C polymorphism does not appear to be related to the risk of cancer.