Analysis of Genetic Variability and Agronomic Performance of Indian Lettuce (Lactuca Indica Linn.)

Lactuca indica is a undomesticated medicinal crop in the Asteraceae family. Present study was carried out to identify elite genotype for cultivation and breeding improvement of lettuce. Data was recorded for 19 morphological and developmental traits across 38 accessions (Acc). In addition to this, the morphological cluster was compared with the simple sequence repeat (SSR) marker. The genotypic mean square variance was significant for all characters. The higher extent of genotypic and phenotypic coefficient of variation obtained for basal branch, leaf blade width, and node number. The broad-sense heritability (H2B) ranged from 45.85% (seed length) to 98.59% (node number), whereas genetic advance as a percentage of the mean (GAM%) ranged from 9.33 to 191. Vegetative characters such as node number, plant hight, basal branch were conjugated with high H2B and high GAM% indicating additive gene effect and selection of these traits based on phenotypic observation is effective for better gain. Reproductive traits, including bolting duration, flowering duration, and seed weight were linked with high H2B, and moderate GAM% trevealing that these traits are amenable to genetic improvement, these traits also showed a significant positive correlation. Acc 55 and 8 showed the best performance for the majority of the attributes could be good material for further research and breeding. In the Wards’ phylogenetic tree of morphological traits, accessions were clustered based on their phenotypic characters rather than the geographic origin. Morphological characteristics showed no or very week corelation with the SSR genotyped data.

The effect of water deficit on some physiological indices of Indian lettuce (Lactuca indica L.)

Indian Lettuce (Lactuca indica L.) is a valuable medicinal herb but there are still no many researches about this plant. In this work, the physiological responses of Indian lettuce plants under water deficit conditions (5, 8, and 11 days of water stress) were investigated. The Indian lettuce wilted after 5 days of water stress (66.66%), the wilting rate increased after 8 (93.33%) and 11 days (100%) of water stress. The longer duration of water deficit stress caused the slower recovery of plants after rewatering. The water deficit stress caused a decrease in chlorophyll fluorescence, non-associated water content as well as flower formation of Indian lettuce. But the water deficit stress increases the associated water content and the flowering time of this plant.

ANTIOXIDANT ACTIVITY TEST of SIJUKKOT (Lactuca indica L.) LEAVES EXTRACT LEAF

Has been investigated the antioxidant activity of sijukkot ( Lactuca indica L.) The antioxidant activity was tested by capturing 2,2’-diphenyl-picrylhydrazyl (DPPH) free radical method and Vitamin C as a standard of comparison, to absorption using UV-Vis spectrophotometry at a wavelength of 517nm. The data obtained were analyzed using the SPSS for windows, resulting in a value of y = 0.2036x + 30.35 for sijukkot extract and y = 29.625x - 73.664 for vitamin C. It was found the leaves have an antioxidant site with 96,51 ppm through IC50 test 4,17 ppm which used Vitamin C as a control.

PHYTOCHEMICAL SCREENING OF SIJUKKOT EKSTRACT (Lactuca Indica L.)

Have been carried out Phytochemical screening on Sijukkot which determited as Lactuca indica L, the plant from Gibeon hill forrest area in the village of Parsaora Sibisa Ajibata, Toba Samosir, North Sumatera. Test carried out to determine the composition of secondary metabolites contained in these plants. It was started by extracting plant leaves using 96% ethanol solvent which was carried out by maceration method and concentrated using a rotary evaporator. The concentrated extract obtained was sponsored by phytochemical tests. Phytochemical test results on ethanol extracts from the leaves of the Sijukkot plant were obtained compositions namely Flavonoids, tannins, saponins, steroids and triterpenoids.

Isolasi dan Identifikasi Senyawa Flavonoid dalam Daun Kumak (Lactuca indica L.)

Pendahuluan: Secara tradisional Lactuca indica L. digunakan untuk menambah nafsu makan, memperlancar pencernaan menambah stamina, mengobati penyakit gondok, mengobati sakit lambung, menurunkan kolesterol, kadar gula darah dan risiko kanker. Hasil pemeriksaan skrining fitokimia simplisia dan ekstrak dijumpai adanya senyawa flavonoid, tanin, saponin, glikosida dan triterpenoid/ steroid. Tujuan: Untuk mengisolasi senyawa flavonoida dari ekstrak Daun Kumak lalu mengidentifikasi golongan senyawa dengan spektrofotometri UV-Vis. Metode: Ekstraksi dilakukan dengan maserasi bertingkat menggunakan pelarut etanol 80%, selanjutnya ekstrak etanol difraksinasi dengan etil asetat dan terakhir dengan n-heksana. Flavonoida diisolasi menggunakan kromatografi kertas (Kkt) dan isolat dikarakterisasi dengan spektrofotometri UV-Vis. Hasil: Isolat berfluoresensi biru lemah dibawah lampu UV 366 nm, hasil spektrofotometri Uv-Vis terdapat dua puncak pada λ maksimum 336,2 nm (pita I) dan 271,2 nm (pita II). Kesimpulan: Diduga isolat merupakan golongan flavonol, yang dapat dilihat dari rentang panjang gelombangnya yaitu antara 350-385 nm (pita I) dan 250-280 nm (pita II).