Effects of a symbiotic on coccidian infestation and zootechnical performances in broilers

Coccidiosis remains an obstacle for the poultry sector worldwide, including Algeria. In the field of food biosecurity, symbiotics are used with the aim of improving zootechnical performance of chickens, and combatting the negative effects of this parasitosis. This study compared two broiler farms with 12,000 chickens of the Arbor Acres strain, where chickens on one farm received supplementation with symbiotics. Weight of 144 animals, mortality, food and water consumption were measured, and consumption (CI) and conversion indices were calculated. For parasitology, droppings were collected and the intestinal contents of 10% of animals were collected. Data showed that at 43 days, the supplemented group had a weight of 2750 g as compared to the control that had a weight of 2630 g, with an average consumption of 4834 g vs. 5087g per animal, CI of 1.62–1.35–0.81 vs. 1.65–1.52–0.89, the conversion index of 1.9 vs. 2.07, and mortality rate of 9.29% vs. 10.37%, respectively. Oocyst excretion was zero at the farm receiving supplementation vs. 0, 100, and 550 OPG in the control group on days 15, 21 and 28, respectively, parasite infestation was 41,600 OPG, 32,800 OPG, and 30,500 OPG vs. 800 OPG, 1,100 OPG and 1,200 OPG, respectively. For prevalence, several species of Eimeria were identified in the control. The means found in this study support the use of symbiotics, and efforts to improve the formula of the symbiotics should yield even better results.

Bacteriome Diversity of Blackflies’ Gut and Association with Onchocerca volvulus, the Causative Agent of Onchocerciasis in Mbam Valley (Center Region, Cameroon)

Vector control using larvicides is the main alternative strategy to address limits of preventive chemotherapy using ivermectin for the control of onchocerciasis. However, it remains substantially limited by implementation difficulties, ecological concerns and the resistance of vector populations. Therefore, efficient and environmentally safe alternative control strategies are still needed. This study explores the composition of the blackfly bacteriome and its variability in the presence of Onchocerca volvulus infection, in order to determine their potential as a novel vector control-based approach to fight onchocerciasis. An entomological survey of a collection of samples was performed in the Bafia health district, a historical endemic focus for onchocerciasis in Cameroon. A total of 1270 blackflies were dissected and the infection rate was 10.1%, indicative of ongoing transmission of onchocerciasis in the surveyed communities. Sequencing process of blackflies’ gut DNA for bacteria screening revealed 14 phyla and 123 genera, highlighting the diversity of gut blackflies bacterial communities. Eight bacteria formed the core of blackfly bacteriome and Wolbachia was the predominant genus with 73.4% of relative abundance of blackflies’ gut bacterial communities. Acidomonas and Roseanomas genera were significantly abundant among infected blackflies (p = 0.01), whereas other genera such as Brevibacterium and Fructobacillus were associated with the absence of infection (p = 0.0009). Differences in gut bacterial distribution of blackflies according to their infection status by the parasite suggest a causal relationship between the bacteriome composition and the onset of blackflies’ infection by O. volvulus or vice versa. Blackfly native bacteria are then potentially involved in infection by O. volvulus, either by facilitating or preventing the parasite infestation of the vector. These bacteria represent an interesting potential as a biological tool/target for a novel approach of vector control to fight onchocerciasis.

Occurrence of Neoechinorhynchus curemai (Acanthocephala: Neoechinorhynchidae) in Prochilodus nigricans (Characiformes: Prochilodontidae), in southwestern Amazon

Prochilodus nigricans is extensively exploited in fishing and aquaculture activities in the Brazilian Amazon, it is the definitive host for Neoechinorhynchus curemai Noronha, 1973. Thus, the present study aimed to evaluate the occurrence of N. curemai in P. nigricans and the parasite-host relationship in three rivers (Juruá, Crôa and Môa) in the municipality of Cruzeiro do Sul, state of Acre, Brazil. Fish were caught, weighed, measured, and subjected to necropsy, and the gastrointestinal tract and viscera were analyzed. A total of 178 specimens of N. curemai were found in 61 infected fish, with the (p= 58.62%). The prevalence, mean intensity, and mean abundance were higher in hosts from the Môa River, and lower from the Juruá River. Regarding the length-weight relationship, the b-value did not differ statistically from three (b=3) for fish species in the three locations, nor in parasitized and non-parasitized species. In addition, growth was considered isometric, and in the case of the relative condition factor, there was no difference in fish hosts between the three rivers. The correlation between parasite intensity, condition factor, length, and weight of P. nigricans was not significant. Thus, this parasite infestation varied between the habitats. However, this did not influence the growth and development of the hosts.

Effect of Sanitation Interventions on Health Outcomes: A Systematic Review of Cluster-Randomized Controlled Trials in Rural Communities of Low- and Middle-Income Countries

A systematic review of published literature (2000–2019) evaluating the impact of sanitation interventions on the prevalence of disease, parasite infestation, and/or child growth using randomized controlled trials (RCTs) was done according to the PRISMA checklist. Earlier reviews indicated mixed evidence citing relatively poor quality evidence from mixed designs. Public health policy and practice appear to rely on evidence from RCTs. Records were searched in six electronic databases. The methodological quality of RCTs was assessed using the Cochrane collaboration risk of bias tool. Fifteen records (2.0%) were included for review. Impact trials were done in rural communities of African and Asian countries. The significant effect of sanitation-focus interventions was found in one trial for the prevalence of childhood diarrhea (14.3%), three trials for parasite infestation (37.5%), and two trials (25.0%) for child growth. Results indicate mixed quality evidence from RCT designs. Evidence is limited and suggestive of the impact of sanitation on parasite infestation and child growth. Further rigorous sanitation intervention trials under varying settings are needed to show what really works and under what settings. Future work may explore sanitation behavior change strategies and latrine options to address the challenges of poor latrine use under high sanitation coverage.

Pemeriksaan dan Pendidikan Pencegahan Pedikulosis Kapitis pada Santri Pesantren Jabal Nur Kecamatan Kandis, Kabupaten Siak, Provinsi Riau

Pediculosis capitis is an infectious parasitic disease which is still a healthproblem, especially for people who live together in groups. Pesantren is a placewhere a group of students study and live together. They carry out daily activitiestogether, from sleeping, eating, studying and playing. This student activity is one ofthe risk factor of contracting a parasite infestation. This activity aims to determinethe incidence of pediculosis capitis, conduct health education, namely counselingabout the eradication of the disease and providing treatment to students who areinfested with Pediculus humanus capitis. This activity was carried out at thePesantren/ Islamic boarding School in Kandis District, Siak Regency. The targetaudience was the sanawiah student were 70 students. There were 36(51,4%)students with pediculosis capitis. Students who had been diagnosed were givenpermethrin 1% and serit comb, after given treatment and education to prevent thedisease, it is hoped that the students will be ableto apply the knowledgegained toeradicate the disease.Keywords: boarding school, pediculosis capitis, Pediculus humanus capitis,studentsAbstrak. Pedikulosis kapitis adalah penyakit parasitik menular yang sampaisekarang merupakan masalah kesehatan terutama pada orang yang tinggal bersamasecara berkelompok. Pesantren merupakan tempat sekelompok santri belajar dantinggal bersama. Mereka melakukan kegiatan sehari-hari bersama, mulai dari tidur,makan, belajar, dan bermain. Kegiatan bersama santri ini merupakan salah saturisiko mudahnya tertular infestasi Pediculus humanus capitis. Kegiatan pengabdiankepada masyarakat ini bertujuan untuk mengetahui kejadian pedikulosis kapitis,melakukan pendidikan kesehatan, yaitu penyuluhan tentang pemberantasan penyakittersebut, dan memberikan pengobatan pada santri yang terinfestasi pedikulosiskapitis. Kegiatan ini dilaksanakan di Pondok Pesantren di Kecamatan Kandis,Kabupaten Siak. Khalayak sasaran adalah santri sanawiah yang berjumlah 70orang. Terdapat 36 (51,4%) santri terkena pedikulosis kapitis. Santri yang sudahdidiagnosis tersebut langsung diberikan permethrin 1% dan sisir serit. Setelahdiberikan pengobatan dan pendidikan pencegahan penyakit tersebut diharapkansantri dapat mempraktikkan pengetahuan yang didapat dalam rangka memberantasinfestasi Pediculus humanus capitis.Kata Kunci: santri, pedikulosis kapitis, Pediculus humanus capitis, pesantren

Analyzing 13 Years of Cetacean Strandings: Multiple Stressors to Cetaceans in Taiwanese Waters and Their Implications for Conservation and Future Research

This study summarizes the postmortem investigations of 73 cetaceans stranded on the coast of Taiwan between 2001 and 2013, including 51 Delphinidae, 17 Kogiidae, 3 Ziphiidae, 1 Physeteridae, and 1 Balaenopteridae. Of these, eight (11%) were categorized into direct human-related strandings, including fisheries interaction (bycatch), vessel collision and other anthropogenic-related pathology. Gastrointestinal foreign bodies were found in eight individuals (11%). Most of the bacteria isolated from stranded dolphins were zoonotic pathogens including extended-spectrum β-lactamases Escherichia coli, which indicates waste pollution from land. Severe parasite infestation was found in 36 of the cases (49%), which suggests that the immune function could be compromised. Thirty-eight cases (52%) were diagnosed with myocardial patchy fibrosis or dilated cardiomyopathy. The evidence shown here indicates that cetaceans around Taiwanese waters may suffer from multiple stressors. This study provides baseline data for the health assessment of cetacean populations in Taiwan, which may ultimately provide recommendations for future cetacean conservation and research throughout the western Pacific.

Epigenetics and Medicine

“Epigenetics is the study of how your behaviors and environment can cause changes that affect the way your genes work. Unlike genetic changes, epigenetic changes are reversible and do not change your DNA sequence, but they can change how your body reads a DNA sequence” (https://www.cdc.gov/genomics/disease/epigenetics.htm). Epigenetic interactions, along with the genetic expression in innate cells, change the structure and function of chromatin, and thus, turn the genes on and off. Epigenetic changes influence disease load and resistance and play an important role in health maintenance and almost all medical disorders, and differs significantly with sex and ethnicity. Epigenetic changes may have either positive or detrimental effects on the immune system. They are long-lasting, increase a host’s susceptibility to infections and medical pathologies, and affect the efficacy of vaccines. Recent studies have indicated that detrimental epigenetic changes can be mended. Safe and effective mechanisms to reverse detrimental epigenetic scars will have broad medical implications, decrease mortality after infections, and protect the elderly against infections, autoimmune diseases, and cancer. These therapies might be useful for the successful application of vaccines in countries where HIV, parasite infestation, malaria, and other chronic diseases are endemic, and also for a better effect of vaccines in geriatric patients.

Repeatable differences in exploratory behaviour predict tick infestation probability in wild great tits

Ecological factors and individual-specific traits affect parasite infestation in wild animals. Ixodid ticks are important ectoparasites of various vertebrate hosts, which include passerine bird species such as the great tit (Parus major). We studied various key ecological variables (breeding density, human disturbance) and phenotypic traits (exploratory behaviour, body condition) proposed to predict tick infestation probability and burden in great tits. Our study spanned 3 years and 12 nest box plots located in southern Germany. Breeding, adult great tits were assessed for exploration behaviour, body condition, and tick burden. Plots were open to human recreation; human disturbance was quantified in each plot as a recreation pressure index from biweekly nest box inspections. Infested individuals were repeatable in tick burden across years. These repeatable among-individual differences in tick burden were not attributable to exploration behaviour. However, faster explorers did have a higher infestation probability. Furthermore, body condition was negatively correlated to tick burden. Recreation pressure was correlated to increased infestation probability, although this relationship was just above the threshold of statistical significance. Our study implies that avian infestation probability and tick burden are each determined by distinct phenotypic traits and ecological factors. Our findings highlight the importance of animal behaviour and human disturbance in understanding variation in tick burden among avian hosts. Significance statement Various abiotic and biotic factors, including personality type, influence tick parasitism in birds, but exactly how all these factors interplay remains unclear. We studied a wild population of great tits over a 3-year period and assessed birds for their exploration behaviour and tick infestation. We found that more explorative great tits were more likely to be infested with ticks. By contrast, faster explorers did not have higher tick burdens. Tick burden was nevertheless moderately repeatable among individuals. Our results imply that animal personality influences the probability of parasite infestation, and that infestation likelihood versus intensity are determined by distinct mechanisms. Our work highlights the importance of animal behaviour to understand parasite infestation in wild populations.