A Bayesian approach to modeling antimicrobial multidrug resistance

Multidrug resistance (MDR) has been a significant threat to public health and effective treatment of bacterial infections. Current identification of MDR is primarily based upon the large proportions of isolates resistant to multiple antibiotics simultaneously, and therefore is a belated evaluation. For bacteria with MDR, we expect to see strong correlations in both the quantitative minimum inhibitory concentration (MIC) and the binary susceptibility as classified by the pre-determined breakpoints. Being able to detect correlations from these two perspectives allows us to find multidrug resistant bacteria proactively. In this paper, we provide a Bayesian framework that estimates the resistance level jointly for antibiotics belonging to different classes with a Gaussian mixture model, where the correlation in the latent MIC can be inferred from the Gaussian parameters and the correlation in binary susceptibility can be inferred from the mixing weights. By augmenting the laboratory measurement with the latent MIC variable to account for the censored data, and by adopting the latent class variable to represent the MIC components, our model was shown to be accurate and robust compared with the current assessment of correlations. Applying the model to Salmonella heidelberg samples isolated from human participants in National Antimicrobial Resistance Monitoring System (NARMS) provides us with signs of joint resistance to Amoxicillin-clavulanic acid & Cephalothin and joint resistance to Ampicillin & Cephalothin. Large correlations estimated from our model could serve as a timely tool for early detection of MDR, and hence a signal for clinical intervention.

Critical current improvement and resistance evaluation of superconducting joint between Bi2223 tapes

We improved the critical current (I c) of the superconducting joint between the Bi2223 tapes by introducing the two-step sintering process. The in-field transport I c of ~ 300 A at 4.2 K and 1 T under a 10−9 Ω criterion was successfully demonstrated. The I c improvement can probably be attributed to the enhancement of the intergrain critical current density for a Bi2223 intermediate layer. Ultra-low in-field joint resistance below 10−14 Ω at 4.2 K and 1 T was also demonstrated using current decay measurement. To our best knowledge, this study is the first to demonstrate a practical level of in-field transport I c and ultra-low in-field joint resistance for the superconducting joint between Bi2223 tapes. We believe that this superconducting joint technology will facilitate development of persistent current mode Bi2223 superconducting magnets.

Modeling and validation of nonlinear voltage-current characteristics of ITER PF joint sample tested in SULTAN facility

The ITER Poloidal Field (PF) coils are wound into double pancakes with NbTi cable-in-conduit conductors, which are connected by joints in shaking hands lap-type configuration. The coils are operating in pulsed mode with a maximum operating current of 55 kA and peak magnetic field of 6.4 T, utilizing electromagnetic load on the conductors and joints. A series of PF qualification joint samples modified in praying hands configuration is measured in the SULTAN facility. For some samples, a nonlinear voltage-current (VI) characteristic is observed during the assessment of joint resistance. The growth of joint resistance versus the B×I product is larger than what is expected from the magneto-resistant copper contribution. Two non-homogeneous contact resistance models are developed and combined to quantitatively evaluate the reason for the nonlinear VI behavior in combination with the relevant power dissipation and current redistribution in the joint. The simulations reveal that, for the particular pre-qualification PFJEU2 sample with resistance variation up to 3.5 nΩ, the most probable reason for the nonlinear VI characteristic is a widely spread defective connection between copper sole and shim. The electromagnetic force involves a separation effect on the mechanically and electrically weakly connected parts, resulting into a varying resistance depending on transport current and background field. The hypothesis and models are validated by an experiment on a similar sample PFJEU3 and a post-mortem examination of the PFJEU2 sample.

Experimental and numerical assessment of CHS-RHS T-joints with chords subjected to axial tensile forces

Hollow steel sections are widely used in the construction industry due to their mechanical properties. Joints used in these structures are the subject of research because of their singular and critical behavior. Joints containing chords with more slender cross-sections and axially loaded are still a challenge for design, especially in joints with circular hollow sections (CHS) in the braces and rectangular hollow sections (RHS) in the chords. In this context, this work aimed to study joints formed by a combination of CHS braces subjected to compression loads and RHS chords axially loaded with tension, welded as T-joints. Experimental tests, a numerical model using finite elements, and a parametric analysis were developed. A new equation for the chord stress function was proposed, including joints containing chords with semi-compact sections in tension. The joint resistance values obtained through the numerical models were compared with the equations from ISO 14346:2013 and with the proposed equation. It was observed that, for the numerical models with geometric properties inside the normative validity ranges of ISO 14346:2013, the mean rate of analytical by numerical joint resistance results was equal to 68%, using either the normative or the proposed equation. In the same way, for models outside the current validity ranges, either the proposed equation or the modified equation from ISO 14346:2013 could be used to design CHS-RHS T-joints with the geometric and material properties analyzed.

Laser Ablation of Titanium Alloy (Ti64): Effects of Process Parameters on Performance of Laser Welded Ti64 – Polyamide 6.6 Joints.

Laser welding of metals – polymers has gained strong scientific and industrial interest because of its ability to produce miniaturized joints in lightweight products with customized properties. Surface pretreatments before joining process have shown significant impact on enhancing properties of laser welded metal – polymer joints. This work adopts a Design of Experiments (DoE) approach to investigate the influence of titanium alloy (Ti64) laser ablation parameters on the performance of laser welded Ti64 – polyamide (PA6.6) assemblies. In this first study, significant laser ablation parameters were highlighted, process window outlined, and optimal parameters identified. Laser ablation pretreatment parameters demonstrated a strong influence on joint resistance to failure. Effects of laser ablation parameters on titanium surface morphology were analyzed using Scanning Electron Microscope (SEM). In a second study, the effects of ablation parameters on Ti64 surface properties and welding quality will be investigated.

Low-volume acute multi-joint resistance exercise elicits a circulating brain-derived neurotrophic factor response but not a cathepsin B response in well-trained men

This study examined if acute multi-joint resistance exercises (RE; back squat, bench press, and deadlift) to volitional failure elicited a postexercise increase in the circulating response of biomarkers associated with neuroprotection. Thirteen males (age: 24.5 ± 3.8 years, body mass: 84.01 ± 15.44 kg, height: 173.43 ± 8.57 cm, training age: 7.1 ± 4.2 years) performed 4 sets to failure at 80% of a 1-repetition maximum on the squat, bench press, and deadlift in successive weeks. The measured biomarkers were brain-derived neurotrophic factor (BDNF), insulin-like growth factor 1 (IGF-1), cathepsin B (CatB), and interleukin 6 (IL-6). Biomarkers were assessed immediately before and 10-min after exercise. There was a main time effect (pre-exercise: 24.00 ± 0.61 to postexercise: 27.38 ± 0.48 ng/mL; p < 0.01) for BDNF with increases in the deadlift (p = 0.01) and bench press (p = 0.01) conditions, but not in the squat condition (p = 0.21). There was a main time effect (pre-exercise: 0.87 ± 0.16 to postexercise: 2.03 ± 0.32 pg/mL; p < 0.01) for IL-6 with a significant increase in the squat (p < 0.01), but not the bench press (p = 0.88) and deadlift conditions (p = 0.24). No main time effect was observed for either CatB (p = 0.62) or IGF-1 (p = 0.56). In summary, acute multi-joint RE increases circulating BDNF. Further, this investigation is the first to report the lack of a transient change of CatB to an acute RE protocol. Novelty Low-volume RE to failure can increase BDNF. Resistance training does not confer an acute Cat B response.