Composition, Community Structure and Vertical Distribution of Epiphytic Ferns on Bamboo Species In Bogor Botanic Gardens, Indonesia

Ecological study of epiphytic ferns growing on bamboo species in Bogor Botanic Gardens, a man-made ecosystem located in a wet lowland area was carried out. The 350 phorophytes of 35 bamboo clumps belonging to 9 species and 3 genera were observed. Each culm was divided into intervals of 1 m from ground level to a height of 3 m. A total of 1984 individuals belonging to 12 species, nine genera, and six families of epiphytic ferns were recorded, with the highest species richness in Polypodiaceae (7 spp.). Dendocalamus giganteus Munro was the best host for epiphytic ferns (9 spp.). Two dominant species, Asplenium nidus L. and Davallia denticulata (Burm.f.) Mett. ex Kuhn with the same frequency value (97.14%), and relative species abundance of 31.49 individuals per clump and 14.94 individuals per clump, showed the highest Importance Value Index (IVI) of 83.19 and 54.00%, respectively. The first one-meter level of bamboo culms hosted all the species. Pyrrosia piloselloides (L.) Price was the only species that grew until the highest intervals of height with a decreasing frequency from the bottom. Bangladesh J. Bot. 50(4): 1095-1107, 2021 (December)

The Phenetic Relationship of Ferns (Polypodiaceae) at the Ascent of Cemoro Kandang, Mount Lawu

The fern is one of the highly diverse vascular plant species, which has a unique characteristic. The closely related at the family level because the genera within each family have some uniformity so that the relationship and groupings cannot be distinguished. One example of a diverse fern family is Polypodiaceae. Consequently, it is necessary to simplify the classification; one of them is through phenetic relationship analysis. This study aimed to analyze the phenetic relationship of the Polypodiaceae ferns in Climbing Cemoro, Mouth Lawu enclosure, Karangayar Regency, based on the similarity index on the dendogram. The data were collected using the exploration method and the sporophyte morphological characters were observed. The observations obtained 13 variations of characters. The relationship analysis was carried out with the hierarchical cluster program using SPSS 23. There were six species found from the Polypodiaceae family, there were including Belvisia mucronata, Goniophlebium sp, Lepisorus sp, Pyrrosia piloselloides, Crypsinus taeniatus and Drynaria sparsisora. The finding suggests that six species are divided into two clusters, the Group I consisting of Goniophlebium sp, Lepisorus sp, Lepisorus sp, and Pyrrosia pilloselloides with a similarity coefficient index of 53% and Group II consisting of Drynaria sparsisora, Goniophlebium sp, and Pyrrosia piloselloides with a similarity index of 46%.

IDENTIFIKASI TUMBUHAN PAKU (PTERIDOPHYTA) DI KAWASAN IJEN BANYUWANGI

Kawasan ijen merupakan daerah pegunungan yang memiliki luas wilayah sekitar 2.560 Ha, termasuk hutan wisata seluas 92 Ha dan memiliki ketinggian hingga mencapai 2.799 mdpl, Kawasan Ijen sendiri merupakan gunung api yang masih aktif, dengan karakteristik lingkungan yang masih alami dan cenderung lembab, sehingga memungkinkan berbagai tumbuhan paku (Pteridophyta) dapat hidup baik di kawasan tersebut. Penelitian ini bertujuan untuk mengetahui jenis spesies tumbuhan paku (Pteridophyta) yang ada di Kawasan Ijen Banyuwangi dan mengetahui hasil deskripsi spesies tumbuhan paku (Pteridophyta) yang ada di Kawasan Ijen Banyuwangi. Data hasil penelitian yang ditemukan untuk di identifikasi. Metode yang digunakan adalah belt transek dengan menggunakan data primer dan data sekunder. Analisis data penelitian dilakukan secara deskriptif yang ditampilkan dalam bentuk gambar dan tabel. Hasil penelitian menunjukkan bahwa terdapat 10 jenis spesies yang termasuk dalam 6 famili, antara lain Pteris biaurita L.; Pteris vitata L.; Nephrolepis cordifolia (L.) Presl; Nephrolepis hirsutula (forst); Pyrrosia longifolia (Burm.F.) C.V. Morton; Pyrrosia piloselloides (L.) M. Price; Goniophlebium korthalsi (Medd) Bedd; Hypolepis punctata (Thunb) Mett ex Kuhn; Cyclosorus heterocarpus (Blume) Ching; Davalia denticulata (Burm.F.) Mett ex Khun.

EXPLORATION OF ENDOPHYTIC FUNGI OF DRAGON SCALE’S FERN (Pyrrosia piloselloides (L.) M.G. Price) AS AN ANTIBACTERIAL SOURCES

Endophytic fungi are fungi which live inside the host plant tissue and have been undergone a horizontal gene transfer process. Endophytic fungi are able to synthesize the same bioactive compounds which synthesized by their host plants. The host plant used in this research was dragon’s scales fern (Pyrrosia piloselloides (L.) M.G. Price). Dragon’s scales fern produces various of bioactive compounds which used as antibacterial agents such as polyphenols. This research was aimed to obtain endophytic fungi isolates from trophophyll fronds and sporophyll fronds of dragon’s scales fern, to determine the antibacterial activity of the secondary metabolite extracts of endophytic fungi, to determine the Minimum Inhibitory Concentration (MIC), to determine the characteristics of the endophytic fungi isolates which potentially as antibacterial source. Based on the research, 13 endophytic fungi isolates were obtained from dragon’s scales fern fronds consist of 5 isolates from trophophyll fronds and 8 isolates from sporophyll fronds. The antibacterial activity test showed that the extract of secondary metabolites of the isolate DTP2 had the highest inhibition zone diameter against E.coli 14.82 ± 4.05 mm, DTP4 against S.aureus 8.80 ± 0.03 mm and DSP4 against S.dysentriae 10.15 ± 0.36 mm. MIC of ethyl acetate extracts of secondary metabolites of isolate DTP2 against E.coli was 125 µg/mL, DTP4 against S.aureus was 125 µg/mL and DSP4 against S.dysentriae was 31.25 µg/mL. The endophytic fungi isolate DTP2 identified as Aureobasidium melanogenum, DTP4 identified as Penicillium alliisativi and DSP4 identified as Aspergillus flocculosus.

Diversity and host preferences of ferns and lycopods epiphytes on palm trees

Praptosuwiryo TN, Sumanto, Cahyaningsih. 2019. Diversity and host preferences of ferns and lycopods epiphytes on palm trees. Biodiversitas 20: 3731-3740. Epiphytic ferns contribute importantly to the global biodiversity of tropical rain forests. However, our knowledge of the ecology of epiphytic ferns is phenomenally still limited. Most studies on the ecology, floristic and diversity of epiphytic ferns and lycopods were reported outside of Malesian region. This study aimed: (i) to figure out the diversity of ferns and lycopods on palm trees of the lowland areas by analyzing its species richness and floristic composition, and (ii) to determine the host preferences of epiphytic ferns on palm trees. Twenty-eight phorophyte species belonging to 17 genera of palm trees, growing at Bogor Botanic Gardens, were observed. Trunk of palm trees was divided into three zones (basal, middle and upper). Individual numbers for each species of epiphytic fern growing on each zone were recorded. Epiphytic fern species which were most commonly found were analyzed statistically to determine their preference for the host trees. A total of 16 species of epiphytic ferns and lycopods were recorded. The greatest richness of species occurred in Polypodiaceae (50%). Habitual true epiphyte was the predominant ecological category, representing 75 % of the species. Caryota no Becc. hosted the greatest number of ferns and lycopods 8 species) while Attalea insignis (Mart.) Drude hosted the fewest (2 species). Individual numbers of six epiphytic fern species were significantly affected by palm trees species, namely Belvisia callifolia (Christ) Copel., Pyrrosia lanceolata (L.) Farwell, Pyrrosia piloselloides (L.) M.G. Price, Davallia denticulata (Burm.) Mett., Nephrolepis biserrata (Sw.) Schott, Vittaria ensiformis Sw. Individual numbers of five epiphytic fern species were significantly affected by zone of the host plant. Nephrolepis biserrata tends to grow well in the upper zone. Pyrrosia lanceolata tends to be abundant in the middle zone. B. callifolia, D. denticulata, and V. ensiformis grow well and make dense populations in the basal zone.

DIVERSITY OF EPIPHYTIC FERN ON THE OIL PALM PLANTS (Elaeis Guineensis Jacq.) IN CAMPUS OF SRIWIJAYA UNIVERSITY INDRALAYA

Campus of Sriwijaya University in Indralaya , has a high diversity of flora including fern, especially the epiphytic ferns, whose existence is so widely found in oil palm plants. Survey about diversity of this epiphytic has been carried out from July to November 2017, with aim to identifying the diversity of species of epiphytic ferns in oil palm plants on the campus of UNSRI Indralaya. The research method used is exploration method with direct collection technique from the field and then made herbarium. A total of 27 species of pteridophytes were collected from the study area. Floristic analysis of collected specimens was carried out to find out the families, genus and species. In this survey was collected 27 species belonged to13 genera, 7 families such Aspleniaceae, Blechnaceae, Davalliaceae, Lygodiaceae, Nephrolepidaceae, Polypodiaceae, and Vittariaceae. The most common types are Polypodiaceae is Drynaria quercifolia, Drynaria sparsisora, Microsorum pustulatum, Microsorum punctatum, Phymatosorus scolopendria, Polypodium verrucosum, Polypodium polysthicum, Pyrrosia piloselloides, and Goniophlebium verrucossum. The most widely distributed species in oil palm is Davallia denticulata , Goniophlebium verrucosum, and Nephrolepis biserrata.

UJI AKTIVITAS ANTIPIRETIK EKSTRAK DAUN SISIK NAGA(Pyrrosia piloselloides (L.) M.G. Price) TERHADAP TIKUS PUTIH (Rattus norvegicus) JANTAN GALUR WISTAR YANG DIINDUKSI PEPTON 5%

INTISARI Demam adalah keadaan ketika suhu tubuh meningkat melebihi suhu tubuh normal. Demam terjadi karena pelepasan pirogen dari dalam leukosit yang sebelumnya telah terangsang oleh pirogen eksogen yang dapat berasal dari mikroorganisme atau merupakan suatu hasil reaksi imunologik yang tidak berdasarkan suatu infeksi. Sementara itu, banyak negara telah mengembangkan pengobatan menggunakan herbal. Salah satu usaha yang dilakukan adalah dengan penggunaan obat tradisional. Penelitian ini bertujuan untuk membuktikan bahwa daun Sisik Naga (Pyrrosia piloselloides (L.) M.G Price) dengan konsentrasi 0,062%, 0,125% dan 0,25% memiliki aktivitas antipiretik dengan menggunakan penginduksi demam Pepton 5%. Penelitian ini merupakan penelitian eksperimental dengan Rancang Acak Lengkap (RAL). Pengukuran suhu rektal tikus menggunakan termometer infra red. Hewan uji dibagi menjadi 5 kelompok yaitu kelompok kontrol negatif (suspensi Na-CMC 1%), kelompok kontrol positif (suspensi parasetamol) dan kelompok uji yaitu pemberian ekstrak daun sisik naga 0,062%, 0,125% dan 0,25%. Data yang diperoleh dianalisis dengan menggunakan uji anova dan uji LSD (Least Significant Different). Hasil dari penelitian ini bahwa ekstrak daun sisik naga (Pyrrosia piloselloides (L.) M.G Price) konsentrasi 0,25% mempunyai aktivitas antipiretik pada tikus putih (Rattus norvegicus) jantan galur wistar. Kata kunci: Antipiretik, Daun Sisik Naga (Pyrrosia piloselloides (L.) M.G Price), Pepton 5% ABSTRACT A fever is a condition when the body temperature rises beyond the normal body temperature. Fever occurs due to the release of pyrogytes from within leukocytes that have previously been stimulated by exogenous pyrogens that may originate from microorganisms or are a result of immunologic reactions that are not based on an infection. Meanwhile, many countries have developed treatments using herbs. One of the efforts done is with the use of traditional medicine. This study aims to prove that the leaves of the Dragon Scales (Pyrrosia piloselloides (L.) M.G Price) with concentrations of 0.062%, 0.125% and 0.25% have antipyretic activity using Pepton fever induction 5%. This research is an experimental research with Completely Random Design (RAL). Rectal temperature measurements of mice using infra red thermometer. Test animals were divided into 5 groups: negative control group (1% Na-CMC suspension), positive control group (paracetamol suspension) and the test group were 0.062%, 0.125% and 0.25%. The data obtained were analyzed using anova test and LSD (Least Significant Different) test. The results of this study showed that the extract of leaf scales (Pyrrosia piloselloides (L.) M.G Price) concentrations of 0.25% had antipyretic activity in white rats (Rattus norvegicus) male wistar strain. Keywords: Antipyretics, Leaf Scales Dragon (Pyrrosia piloselloides (L.) M.G Price), Pepton 5%