scholarly journals Species diversity and phenetic relationship among accessions of api-api (Avicennia spp.) in Java based on morphological characters and ISSR markers

2020 ◽  
Vol 22 (1) ◽  
Author(s):  
Fennalia putri Sabdanawaty ◽  
Purnomo PURNOMO ◽  
Budi Setyadi Daryono
Keyword(s):  
Species Diversity ◽  
Leaf Shape ◽  
Pcr Amplification ◽  
Morphological Characters ◽  
Issr Markers ◽  
Ecological Benefits ◽  
Phenetic Relationships ◽  
Phenetic Relationship ◽  
Marine Areas ◽  
High Diversity

Abstract. Sabdanawaty FP, Purnomo, Daryono BS. 2021. Species diversity and phenetic relationship among accessions of api-api (Avicennia spp.) in Java based on morphological characters and ISSR markers. Biodiversitas 22: 193-198. Api-api (Avicennia spp.) from mangrove groups is rich in economic and ecological benefits. Research on the potential of api-api has been extensively done, but no research is specifically focused on the identification of api-api. This study aims to identify species diversity and phenetic relationships of api-api in Java based on morphological and molecular characters. The morphological analysis was based on 35 characters and referred to the descriptor. The PCA analysis on the morphological characters of Avicennia spp. showed a high diversity. Morphological characters have a high effect on grouping patterns such as habitat, leaf shape, leaf tip, leaf base, and petal color. Molecular observations were done on the polymorphism of DNA bands. The ISSR primers used were ISSR02, ISSR04, and ISSR10. PCR amplification of DNA was separated and visualized using a doc gel electrophoresis. The results showed a high diversity based on the percentage of DNA polymorphism. The research resulted in three species of Avicennia spp. in Java, namely Avicennia officinalis, A. alba, and A. marina. Phenetic relationships between Avicennia spp. form was based on similarity in character, not based on the area of origin. The environmental conditions in this study slightly opposed the character of Avicennia spp., which presumably due to the similarity in various marine areas of Java such as temperature, pH, and the required salinity.

scholarly journals The Diversity And Classification Of Intraspecies Of Gembolo (Dioscorea Bulbifera L.) Based On Morphological Character

2018 ◽  
Vol 73 ◽  
pp. 04022
Author(s):  
Andri Prasetia ◽  
Purnomo ◽  
Budi Setiadi
Keyword(s):  
Morphological Character ◽  
Leaf Shape ◽  
Similarity Index ◽  
Morphological Characters ◽  
Multivariate Statistical ◽  
Pair Group ◽  
Group I ◽  
Dioscorea Bulbifera ◽  
Phenetic Relationship ◽  
Special Region

Gembolo (Dioscorea bulbifera L.) is a dioecious, annual, herbaceous, climbing plants and has heart-shaped leaves. Gembolo has aerial tubers (bulbil) and main tuber that has irregular shapes, as well as many rough roots at the base of the stem. The purpose of this research is to know the phenetic relationship of the germplasm of Gembolo in Special Region of Yogyakarta based on morphological character. The results of this study are expected to provide information on intraspecies diversity, phenetic relationship and Gembolo distribution. Gembolo plant samples were taken from D.I Yogyakarta. The sample was observed based on the difference of morphological character, so the number of accessions obtained from D.I Yogyakarta could be determined. Morphological characters would be described and characterized to determine the Operational Taxonomic Units (OTU’s). Based on morphological character data, then the grouping analysis was done by grouping analysis method and the dendrogram was formed by the method of Unweighted Pair Group Methods using Arithmetic averages (UPGMA) using Multivariate Statistical Program (MVSP) software version 3.1pc. The results showed that gembolo had morphological variation in tuber shape, stem color, leaf shape, leaf base shape and leaf tip shape. Based on the morphological characters, 2 main groups with the value of similarity index of 62,8% was formed. Group I consisted of four accessions with a similarity value of 85%. Group II consisted of eight accessions with a similarity value of 75%. A high similarity based on the morphological character on gembolo accession caused the gembolo plants in Special Region of Yogyakarta did not vary.

scholarly journals Identification of Species-Diagnostic ISSR Markers for Six Eucalyptus Species

2006 ◽  
Vol 55 (1-6) ◽  
pp. 119-122 ◽  
Author(s):  
T. Balasaravanan ◽  
P. Chezhian ◽  
R. Kamalakannan ◽  
R. Yasodha ◽  
M. Varghese ◽  
...  
Keyword(s):  
Wood Quality ◽  
Natural Populations ◽  
Pcr Amplification ◽  
Morphological Characters ◽  
Issr Markers ◽  
Diagnostic Markers ◽  
Raw Material ◽  
Eucalyptus Species ◽  
Breeding Programs ◽  
Identification Of Species

Abstract Eucalyptus is planted worldwide for raw material in paper and rayon industry. It is a potential out-crosser and the natural populations are highly heterogeneous displaying strong inbreeding depression. Eucalyptus hybrids have been intensively utilized for their vigor, higher wood quality and resistance to diseases. Identification of species for hybridization is predominantly based on morphological characters and is not always reliable. Hence, DNA marker based species identification and hybrid validation is an important and efficient tool in breeding programs. In the present study, attempts were made to identify species - diagnostic markers for six eucalypt species (E. camaldulensis Dehnh, E. citriodora Hook, E. grandis W. Hill ex Maiden, E. pellita F. Muell, E. tereticornis Sm and E. urophylla S.T. Blake) using ISSR-PCR fingerprints. PCR amplification using seven ISSR primers resulted in significant polymorphism among the population from different species. E. citriodora and E. tereticornis showed monomorphic frequency of maximum 37.5% and minimum 14.3% respectively. Twenty species-diagnostic markers were identified for E. camaldulensis, E. citriodora, E. grandis and E. urophylla while no marker was detected for E. pellita and E. tereticornis. A maximum of eleven and a minimum of one species-diagnostic marker were recorded for E. citriodora and E. camaldulensis respectively. Among the twenty markers, nine were present in all the individuals of a particular species.

scholarly journals Short Communication: New record of wild banana (Musa balbisiana Colla) in West Kalimantan, Indonesia

2017 ◽  
Vol 18 (4) ◽  
pp. 1324-1330
Author(s):  
ARI SUNANDAR
Keyword(s):  
New Record ◽  
Morphological Character ◽  
Short Communication ◽  
Morphological Characters ◽  
Musa Balbisiana ◽  
Center Of Origin ◽  
West Kalimantan ◽  
Phenetic Relationships ◽  
Wild Banana ◽  
Phenetic Relationship

Sunandar A. 2017. Short Communication: New record of wild banana (Musa balbisiana Colla) in West Kalimantan, Indonesia. Biodiversitas 18: 1324-1330. Kalimantan Island (Indonesia) is part of the center of origin and diversity of wild bananas in Indo-Malesia region. West Kalimantan is one of province in Kalimantan Island. However, only a little information is available about diversity of wild bananas in West Kalimantan. The aims of this study were to study a possible new record of wild banana species in Nipah Panjang and Teluk Nibung Villages, Kubu Raya District and to describe its phenetic relationships among wild banana species based on morphological characters. By examination based on the morphological character, one species of wild banana was identified, i.e. Musa balbisiana found in Teluk Nibung Village (Local name: Pisang Klotok). However, we didn’t found any wild bananas in Nipah Panjang Village. M. balbisiana was a new record in West Kalimantan. Previously, M. balbisiana is widely distributed only in Java and Sulawesi. M. balbisiana was found in roadsides or backyards of residents in Teluk Nibung Village. Dendrogram of phenetic relationship showed that M. balbisiana from West Kalimantan had similarity with M. balbisiana from Sulawesi with coefficient of similarity 80%.

Diversity and community structure of Ephemeroptera in freshwater stream of Megamalai hills, Tamil Nadu, India

ENTOMON ◽  
2021 ◽  
Vol 46 (4) ◽  
pp. 279-284
Author(s):  
S. Barathy ◽  
T. Sivaruban ◽  
Srinivasan Pandiarajan ◽  
Isack Rajasekaran ◽  
M. Bernath Rosi
Keyword(s):  
Community Structure ◽  
Species Diversity ◽  
Air Temperature ◽  
Tamil Nadu ◽  
Dominant Species ◽  
Diversity Indices ◽  
Low Diversity ◽  
Freshwater Stream ◽  
Species Diversity Indices ◽  
High Diversity

In the study on the diversity and community structure of Ephemeroptera in the freshwater stream of Chinnasuruli falls on Megamalai hills, a total of 523 specimens belonging to thirteen genera and five families were collected in six month periods. Of the five families, Teloganodidae and Leptophlebiidae exhibited high diversity and Caenidae showed low diversity. Choroterpes alagarensis (Leptophlebiidae) is the most dominant species. Diversity indices such as Shannon and Simpson indices showed that diversity was maximum in November and December and it was minimum in August and January. Canonical Correspondence Analysis revealed that rainfall, water flow, turbidity, and air temperature were the major stressors in affecting the Ephemeropteran community structure.

Ostracods as Tsunami Tracers in Holocene Sequences

2010 ◽  
Vol 73 (1) ◽  
pp. 130-135 ◽  
Author(s):  
Francisco Ruiz ◽  
Manuel Abad ◽  
Luís Miguel Cáceres ◽  
Joaquín Rodríguez Vidal ◽  
María Isabel Carretero ◽  
...  
Keyword(s):  
Population Structure ◽  
Species Diversity ◽  
Indian Ocean ◽  
High Energy ◽  
Indian Ocean Tsunami ◽  
Tsunami Deposits ◽  
Shallow Marine ◽  
Juvenile Stages ◽  
Marine Areas ◽  
Age Population Structure

This review analyses the ostracod record in Holocene tsunami deposits, using an overview of the 2004 Indian Ocean tsunami impact on its recent populations and the associated tsunamigenic deposits, together with results from numerous investigations of other Holocene sequences. Different features such as the variability of the local assemblages, population density, species diversity, age population structure (e.g., percentages of adults and juvenile stages) or taphonomical signatures suggest that these microorganisms may be included amongst the most promising tracers of these high-energy events in marshes, lakes, lagoons or shallow marine areas.

scholarly journals MORPHOLOGICAL AND CHROMOSOMAL CHARACTERIZATION OF ORCHID Peristylus goodyeroides Lindl. FROM CURUG SETAWING, KULONPROGO

2021 ◽  
Vol 12 (2) ◽  
pp. 110
Author(s):  
Febri Yuda Kurniawan ◽  
Anindita Della Rosa Riyadi
Keyword(s):  
Leaf Shape ◽  
Ecological Factors ◽  
Morphological Characters ◽  
Morphological Characterization ◽  
Observation Data ◽  
Chromosome Configuration ◽  
Telocentric Chromosomes ◽  
Leaf Arrangement ◽  
Karyotype Formula

Peristylus goodyeroides is a terestrial orchid that scattered around Southeast Asia. Morphological characters of P. goodyeroides can vary, depending on the ecological factors and habitat. Cytological characters in the form of chromosome configurations can be used as a taxonomic tool for the process of identifying and understanding variations in taxa. The purpose of this study was to determine the morphological characters and chromosome configuration of the P. goodyeroides from Curug Setawing, Kulonprogo. The method used was morphological characterization and plant chromosome preparation by squash method with the main steps of fixation, maceration, staining and observation. Data were analyzed with the help of Image raster 3, Corel Draw X7, and Microsoft Excel 2013. P. goodyeroides from Curug Setawing has the morphological characters of root tubers, cylindrical stems, ovate leaf shape, convolutive leaf arrangement and creamy white flowers. The orchid has a number of chromosomes 2n = 10 with a karyotype formula of 2n = 2x = 8m + 2t. Metacentric chromosomes are found on chromosomes 1-8 and telocentric chromosomes 9-10. The absolute arm length of the chromosomes has a range of 2.03-3.44 μm, the relative arm length of the chromosomes is 2.21-3.32 μm, the length of the p arm is 1.13-1.58 μm and the q arm is 1.23-2.12 μm.

scholarly journals Baseline Assessments of Wildlife Biodiversity Within Selected Areas of Central Visayas, Philippines

2021 ◽  
Vol 6 (2) ◽  
pp. 27-34
Author(s):  
RP Jose ◽  
WA Aureo ◽  
CI Narido ◽  
TD Reyes Jr ◽  
MB Sarnowski
Keyword(s):  
Species Richness ◽  
Species Diversity ◽  
Diversity Index ◽  
Population Trends ◽  
Species Richness And Diversity ◽  
Natural Park ◽  
Biodiversity Inventories ◽  
Baseline Information ◽  
High Diversity ◽  
Areas Of Protection

A comprehensive inventory of faunal biodiversity was conducted within three Key Biodiversity Areas of Central Visayas. Mt. Bandilaan Natural Park (MBNP), Balinsasayao Twin Lakes Natural Park (BTLNP), and Rajah Sikatuna Protected Landscape (RSPL). Three primary wildlife groups were surveyed: bats, birds and amphibians. Species diversity and population trends were then analyzed to assess areas of biodiversity importance. RSPL generally had the highest species richness and diversity index while MBNP consistently had the lowest. When pooled. The surveyed areas resulted in a high diversity index and rate of endemicity showing the importance of multiple areas of protection. These findings can be utilized to prioritize habitat protection as well as to serve as a baseline information for future biodiversity inventories. J. Biodivers. Conserv. Bioresour. Manag. 2020, 6(2): 27-34

scholarly journals Screening of homokaryotic protoclones of Agaricus bisporus (J. Lge) Imbach by colony characters and ISSR markers

2010 ◽  
Vol 39 (1) ◽  
pp. 119-122 ◽  
Author(s):  
Mahmudul Islam Nazrul ◽  
Fan Xiao Lin ◽  
Bian Yin-Bing
Keyword(s):  
Simple Sequence Repeat ◽  
Agaricus Bisporus ◽  
Morphological Characters ◽  
Inter Simple Sequence Repeat ◽  
Issr Markers ◽  
Fruiting Bodies ◽  
Sequence Repeat ◽  
Slow Growing ◽  
Simple Sequence ◽  
Issr Primers

Among ten slow-growing protoclones of Agaricus bisporus (J. Lge) Imbach, all appressed colonies showed slower growth rate and spawn run, and inability to produce fruiting bodies in substrate. Seven of 40 inter-simple sequence repeat (ISSR) primers amplified 78 reproducible fragments, 48.93% were polymorphic, each producing 7 to 16 bands ranging from 0.10 to 2.10 kbp, sufficient to differentiate the protoclones from each other. Appressed protoclones were homoallelic at a number of loci that were heteroallelic in the parent, suggesting that they represented rare homokaryons. Thus, using morphological characters along with ISSR, polymorphisms could be useful for quick, easy, and accurate in distinguishing homo- and heterokaryotic isolates. Key words: Agaricus bisporus (J. Lge) Imbach; Homokaryon; ISSR; Protoclone DOI: 10.3329/bjb.v39i1.5537Bangladesh J. Bot. 39(1): 119-122, 2010 (June)

Genetic diversity of Echinacea species based upon amplified fragment length polymorphism markers

Genome ◽  
2004 ◽  
Vol 47 (1) ◽  
pp. 102-111 ◽  
Author(s):  
D -H Kim ◽  
D Heber ◽  
D W Still
Keyword(s):  
Genetic Diversity ◽  
Genetic Distance ◽  
Fragment Length ◽  
Echinacea Purpurea ◽  
Morphological Characters ◽  
Pairwise Distance ◽  
Neighbor Joining ◽  
Aflp Data ◽  
Phenetic Relationships ◽  
Length Polymorphisms

The taxonomy of Echinacea is based on morphological characters and has varied depending on the monographer. The genus consists of either nine species and four varieties or four species and eight varieties. We have used amplified fragment length polymorphisms (AFLP) to assess genetic diversity and phenetic relationships among nine species and three varieties of Echinacea (sensu McGregor). A total of 1086 fragments, of which approximately 90% were polymorphic among Echinacea taxa, were generated from six primer combinations. Nei and Li's genetic distance coefficient and the neighbor-joining algorithm were employed to construct a phenetic tree. Genetic distance results indicate that all Echinacea species are closely related, and the average pairwise distance between populations was approximately three times the intrapopulation distances. The topology of the neighbor-joining tree strongly supports two major clades, one containing Echinacea purpurea, Echinacea sanguinea, and Echinacea simulata and the other containing the remainder of the Echinacea taxa (sensu McGregor). The species composition within the clades differs between our AFLP data and the morphometric treatment offered by Binns and colleagues. We also discuss the suitability of AFLP in determining phylogenetic relationships.Key words: Echinacea, AFLP, genetic distance, phylogeny.

Variation in leaf characteristics among provenances of Eucalyptus camaldulensis Dehn. grown in Zambia

1971 ◽  
Vol 19 (2) ◽  
pp. 237
Author(s):  
J Burley ◽  
PJ Wood ◽  
AS Hans
Keyword(s):  
Principal Component Analysis ◽  
Leaf Shape ◽  
Natural Populations ◽  
Eucalyptus Camaldulensis ◽  
Principal Component ◽  
Morphological Characters ◽  
Leaf Length ◽  
Maximum Temperature ◽  
Ecotypic Variation ◽  
Old Trees

Eight morphological characters of leaves were examined in 2-year-old trees of 25 provenances of Eucalyptus camaldulensis Dehn, grown in a replicated trial in Zambia. Provenances differed significantly in leaf length and width, base angle, and oil gland density but significant effects were attributed to trees within provenances. Leaf curvature also varied between provenances, trees, and branches. The tip angle and the number of veins did not vary significantly. Leaf length and oil gland density were related to maximum temperature at seed source rather than to latitude but the reverse occurred for leaf width. Significant amounts of variation were explained by a linear combination of temperature and latitude but not by longitude, altitude, or rainfall. Information on seven traits was combined in principal component analysis in which the first component (a measure of leaf shape) accounted for 36% of total observed variation while the second (weighted largely on leaf length) accounted for 26%. Mapping of both univariate and multivariate results indicated that there are two major ecotypes and gave some evidence of clinal and ecotypic variation within them. The conclusions support those of other workers both in natural populations in Australia and in exotic populations elsewhere.

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