scholarly journals Biosynthesis of Fe nanoparticles using Alhagi sparsifolia extract for the treatment of colorectal carcinoma in the in vitro condition: A pre-clinical trial study

2021 ◽  
Author(s):  
Yadong Zhou ◽  
Fafu Dou ◽  
Tahani Awad Alahmadi ◽  
Sulaiman Ali Alharbi ◽  
Milton Wainwright
Keyword(s):  
Colorectal Carcinoma ◽  
Cell Lines ◽  
Aqueous Extract ◽  
Mtt Assay ◽  
Iron Nanoparticles ◽  
Alhagi Sparsifolia ◽  
Leaf Aqueous Extract ◽  
Hct 116 ◽  
Human Colorectal Carcinoma ◽  
Ht 29

IntroductionThe preparation and formulation of new chemotherapeutic supplements and drugs with remarkable effects for the treatment of cancer are in the priority of both developing and developed countries. Recently, iron nanoparticles have been used as modern chemotherapeutic drugs for the treatment of several cancers such as leukemia, lung cancer, breast cancer, prostate cancer, etc. In the present study, iron nanoparticles were green-synthesized using the aqueous extract of Alhagi sparsifolia leaf aqueous extract.Material and methodsThe synthesized FeNPs were characterized by analytical techniques including SEM, TEM, UV-Vis., and FT-IR. The anti-human colorectal carcinoma activity of FeNPs was evaluated using MTT assay. In the cellular and molecular part of the recent study, the treated cells with FeNPs were assessed by MTT assay for 48h about the cytotoxicity and anti-human colorectal carcinoma properties on normal (HUVEC) and colorectal carcinoma cell lines i.e. HT-29, HCT 116, HCT-8, and Ramos.2G6.4C10.ResultsThe nanoparticles were formed in a spherical shape in the average size of 47.24 nm. In the antioxidant test, the IC50 of FeNPs and BHT against DPPH free radicals were 161 and 134 µg/mL, respectively. The viability of malignant colorectal cell line reduced dose-dependently in the presence of FeNPs. The IC50 of FeNPs were 250, 293, 276, and 344 µg/mL against HT-29, HCT 116, HCT-8, and Ramos.2G6.4C10 cell lines, respectively.ConclusionsAfter clinical study, iron nanoparticles containing Alhagi sparsifolia leaf aqueous extract may be used to formulate a new chemotherapeutic drug or supplement to treat the several types of human colorectal carcinoma.

scholarly journals Calendula officinalis green-mediated silver nanoparticles: Formulation, characterization and assessment of colorectal cancer activities green-mediated silver nanoparticles: Formulation, characterization and assessment of colorectal cancer activities

2021 ◽  
Author(s):  
Yongchao Xu ◽  
Behnam Mahdavi ◽  
Mohammad Zangeneh ◽  
Akram Zangeneh ◽  
Maryam Qorbani ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Silver Nanoparticles ◽  
Colorectal Carcinoma ◽  
Cell Lines ◽  
Aqueous Extract ◽  
Mtt Assay ◽  
Analytical Techniques ◽  
Spherical Shape ◽  
Calendula Officinalis ◽  
Human Colorectal Carcinoma

IntroductionThe biosynthesis of metal nanoparticles using medicinal plants is not only economical but also environmentally friendly as well as having miscellaneous biomedical applications. In the present study, silver nanoparticles were green-synthesized using the aqueous extract of Calendula officinalis.Material and methodsThe synthesized AgNPs@Calendula officinalis were characterized by analytical techniques including EDX, FE-SEM, XRD, UV-Vis., and FT-IR. The anti-human colorectal cancer activities of AgNPs@Calendula officinalis were evaluated using MTT assay.ResultsThe nanoparticles were formed in a spherical shape in the range of 38.05 to 75.41 nm for the particle size. On the other hand, the MTT assay was run to evaluate anti colorectal cancer activity of AgNPs@Calendula officinalis. In the cellular and molecular part of the recent study, the treated cells with AgNPs@Calendula officinalis were assessed by MTT assay for 48 h about the cytotoxicity and anti-human colorectal carcinoma properties on normal (HUVEC) and colorectal carcinoma cell lines i.e. WiDr, SW1417 [SW-1417], and DLD-1. In the antioxidant test, the IC50 of AgNPs@Calendula officinalis and BHT against DPPH free radicals were 222 and 124 µg/mL, respectively. The viability of malignant colorectal cell line reduced dose-dependently in the presence of AgNPs@Calendula officinalis. The IC50 of AgNPs@Calendula officinalis were 430, 326, and 392 µg/mL against WiDr, SW1417 [SW-1417], and DLD-1 cell lines, respectively.ConclusionsAfter the clinical study, silver nanoparticles containing Calendula officinalis leaf aqueous extract may be used to formulate a new chemotherapeutic drug or supplement to treat the several types of human colorectal carcinoma.

scholarly journals Equisetum arvense L aqueous extract a novel chemotherapeutic supplement for the treatment of human colon carcinoma

2021 ◽  
Author(s):  
Lei Wang ◽  
Luojun Zhang ◽  
Guangtao Zheng ◽  
Haiping Luo ◽  
Attalla F. El-kott ◽  
...  
Keyword(s):  
Colorectal Carcinoma ◽  
Cell Line ◽  
Cell Lines ◽  
Mtt Assay ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Total Phenolic ◽  
Equisetum Arvense ◽  
Human Colorectal Carcinoma ◽  
Ht 29

IntroductionOne of the plants that has long been considered by humans is Equisetum arvense L Equisetum arvense L is now recommended for external use to heal wounds and for internal use to relieve urinary tract and prostate disorders.Material and methodsIn the current study, the antioxidant, cytotoxicity, and anti-human ling cancer properties of Equisetum arvense were investigated in the in vitro condition. Total phenolic content, total flavonoid content, radical scavenging activity, and ferrous ion chelating were run to evaluate the antioxidant activity. MTT assay was chosen to investigate anticancer activity of the plant extract.ResultsThe plant extract scavenged DPPH as a free radical with an IC50 of 12.3±0.7 µg/mL better than positive controls. The plant also was rich in phenolic compounds with an amount of 396.2±3.2 mg GAE/g for total phenolic content. In the MTT assay, human colorectal carcinoma (HCT-8 [HRT-18], Ramos.2G6.4C10, HT-29, and HCT 116) and normal cell lines (HUVEC) were used to study the cytotoxicity and anticancer potential of human colorectal over the Equisetum arvense L. The cell viability of Equisetum arvense L was very low against human colorectal carcinoma cell lines without any cytotoxicity on the normal (HUVEC) cell line.ConclusionsThe best anti-human colorectal carcinoma properties of Equisetum arvense L against the above cell lines was in the case of HT 29 cell line.

scholarly journals Di-2-pyridylketone 4, 4-dimethyl-3-thiosemicarbazone effectively induces human colorectal carcinoma cell apoptosis via mTOR pathway

2021 ◽  
Vol 3 (3) ◽  
pp. 56-62
Author(s):  
Qianqian Fu ◽  
Keyword(s):  
Colorectal Carcinoma ◽  
Tumor Cell ◽  
Cell Lines ◽  
Colorectal Tumor ◽  
Iron Chelators ◽  
Tumor Cell Lines ◽  
Human Colorectal Carcinoma ◽  
Colorectal Tumor Cell ◽  
Ht 29 ◽  
Additional Iron

Background: To investigate the anticancer mechanisms of di-2-pyridylketone 4,4-dimethyl-3-thiosemicarbazone (Dp44mT) in human colon cancer cells. Human colorectal carcinoma (HCC) is one of the most commonly diagnosed cancers in both males and females. Current studies have found that iron chelators can be used as novel anticancer drugs; however, the anticancer activity of iron chelators and their target genes in HCC has been rarely reported. Methods: Dp44mT was used to treat two colorectal tumor cell lines, SW480 and HT-29. The proapoptotic effects of different concentrations of Dp44mt were measured using flow cytometry and Hoechst 33258 staining. Ferric ammonium citrate (FAC) was used as an additional iron donor to inhibit the effects of Dp44mT. Apoptosis and DNA damage-related proteins were examined by Western blot analysis. Results: In this study, we found that the iron chelators Dp44mT could induce the apoptosis in two colorectal tumor cell lines SW480 and HT-29, upregulate the expression level of p-histone H2A.X, and inhibit the phosphorylation level of mTOR in a dose-dependent way. Those effects could be reversed by the additional iron donor FAC. Conclusion: These data indicate that iron depletion and/or the presence of iron can modulate the HCC apoptosis progression in vitro, which may be a potential target for future HCC therapy.

scholarly journals Aqueous Extract ofSolanum nigrumLeaves Induces Autophagy and Enhances Cytotoxicity of Cisplatin, Doxorubicin, Docetaxel, and 5-Fluorouracil in Human Colorectal Carcinoma Cells

2013 ◽  
Vol 2013 ◽  
pp. 1-12 ◽  
Author(s):  
Chen-Jei Tai ◽  
Chien-Kai Wang ◽  
Cheng-Jeng Tai ◽  
Yi-Feng Lin ◽  
Chi-Shian Lin ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Colorectal Carcinoma ◽  
Aqueous Extract ◽  
Tumor Suppression ◽  
Drug Effect ◽  
Carcinoma Cells ◽  
Common Cancer ◽  
Human Colorectal Carcinoma ◽  
And Control ◽  
Ht 29

Colorectal cancer is a common cancer worldwide, and chemotherapy is a mainstream approach for advanced and recurrent cases. Development of effective complementary drugs could help improve tumor suppression efficiency and control adverse effects from chemotherapy. The aqueous extract ofSolanum nigrumleaves (AE-SN) is an essential component in many traditional Chinese medicine formulas for treating cancer, but there is a lack of evidence verifying its tumor suppression efficacy in colorectal cancer. The purpose of this study is to evaluate the tumor suppression efficacy of AE-SN using DLD-1 and HT-29 human colorectal carcinoma cells and examine the combined drug effect when combined with the chemotherapeutic drugs cisplatin, doxorubicin, docetaxel, and 5-fluorouracil. The results indicated that AE-SN induced autophagy via microtubule-associated protein 1 light chain 3 A/B II accumulation but not caspase-3-dependent apoptosis in both cell lines. The IC50s after 48 hours of treatment were 0.541 and 0.948 mg/ml AE-SN in DLD-1 and HT-29, respectively. AE-SN also demonstrated a combined drug effect with all tested drugs by enhancing cytotoxicity in tumor cells. Our results suggest that AE-SN has potential in the development of complementary chemotherapy for colorectal cancer.

Soluble Epoxide Hydrolase as an Important Player in Intestinal Cell Differentiation

2020 ◽  
Vol 209 (4-6) ◽  
pp. 177-188
Author(s):  
Katerina Cizkova ◽  
Katerina Koubova ◽  
Tereza Foltynkova ◽  
Jana Jiravova ◽  
Zdenek Tauber
Keyword(s):  
Cell Differentiation ◽  
Colorectal Carcinoma ◽  
Cell Lines ◽  
Epoxide Hydrolase ◽  
Soluble Epoxide Hydrolase ◽  
Intestinal Cell ◽  
Caco2 Cells ◽  
Important Player ◽  
Ht 29

There is growing evidence that soluble epoxide hydrolase (sEH) may play a role in cell differentiation. sEH metabolizes biologically highly active and generally cytoprotective epoxyeicosatrienoic acids (EETs), generated from arachidonic acid metabolism by CYP epoxygenases (CYP2C and CYP2J subfamilies), to less active corresponding diols. We investigated the effect of sEH inhibitor (TPPU) on the expression of villin, CYP2C8, CYP2C9, CYP2J2, and sEH in undifferentiated and in vitro differentiated HT-29 and Caco2 cell lines. The administration of 10 μM TPPU on differentiated HT-29 and Caco2 cells resulted in a significant decrease in expression of villin, a marker for intestinal cell differentiation. It was accompanied by a disruption of the brush border when microvilli appeared sparse and short in atomic force microscope scans of HT-29 cells. Although inhibition of sEH in differentiated HT-29 and Caco2 cells led to an increase in sEH expression in both cell lines, this treatment had an opposite effect on CYP2J2 expression in HT-29 and Caco2 cells. In addition, tissue samples of colorectal carcinoma and adjacent normal tissues from 45 patients were immunostained for sEH and villin. We detected a significant decrease in the expression of both proteins in colorectal carcinoma in comparison to adjacent normal tissue, and the decrease in both sEH and villin expression revealed a moderate positive association. Taken together, our results showed that sEH is an important player in intestinal cell differentiation.

scholarly journals Cytotoxic Constituents of Pachyrhizus Tuberosus from Peruvian Amazon

2013 ◽  
Vol 8 (10) ◽  
pp. 1934578X1300801 ◽  
Author(s):  
Olga Leuner ◽  
Jaroslav Havlik ◽  
Milos Budesinsky ◽  
Vladimir Vrkoslav ◽  
Jessica Chu ◽  
...  
Keyword(s):  
Cell Lines ◽  
Cancer Cell ◽  
Mtt Assay ◽  
Human Cancer ◽  
Chemical Constituents ◽  
Human Fibroblasts ◽  
Cancer Cell Lines ◽  
Cytotoxic Effects ◽  
Hct 116 ◽  
Mcf 7

Investigations into the chemical constituents of the seeds of the neglected tuber crop Pachyrhizus tuberosus (Leguminosae) resulted in the isolation of seven components: five rotenoids [12a-hydroxyerosone (1), 12a-hydroxydolineone (2), erosone (3), 12a-hydroxyrotenone (4) and rotenone (6)], a phenylfuranocoumarin [pachyrrhizine (5)] and an isoflavanone [neotenone (7)]. The compounds were isolated using several chromatography techniques and characterized and verified by NMR and HPLC/MS. The MTT assay was used to examine the selective cytotoxic effects of the methanolic P. tuberosus extract and isolated compounds in two human cancer cell lines [breast (MCF-7) and colorectal (HCT-116)] and in non-transformed human fibroblasts (MRC-5); IC50 values were calculated. The methanolic P. tuberosus extract displayed respectable cytotoxic effects against HCT-116 and MCF-7 cells with IC50 values of 7.3 and 6.3 μg/mL, respectively. Of the compounds, 6 exacted greatest cytotoxicity and selectivity towards the cancer cell lines tested, yielding IC50 values of 0.3 μg/mL against both MCF-7 and HCT-116 cells, and a 6-fold reduced activity against MRC-5 fibroblasts. Compound 4 also demonstrated cytotoxicity against MCF-7 and HCT-116 (1.1 and 1.8 μg/mL, respectively), and reduced cytotoxicity towards MRC-5 cells (7.5 μg/mL). The results revealed from the in vitro cytotoxic MTT assay are worthy of further antitumor investigation.

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