scholarly journals Determination of the quantitative content of some groups of phenolic compounds in tinctures from the raw material of plant families Polygonaceae, Rosaceae, Asteraceae

2021 ◽  
Vol 19 (4(76)) ◽  
pp. 54-59
Author(s):  
Tetiana V. Oproshanska ◽  
Olga P. Khvorost
Keyword(s):  
Hydroxycinnamic Acids ◽  
Raw Material ◽  
Total Polyphenols ◽  
Rosa Canina ◽  
Arctium Lappa ◽  
Quantitative Content ◽  
Bidens Tripartita ◽  
Plant Raw Material ◽  
Sanguisorba Officinalis ◽  
Arctium Minus

Aim. To determine the quantitative content of total polyphenols and the amount of hydroxycinnamic acids in the series of tinctures from rhizomes with roots of Rumex confertus Willd., Sanguisorba officinalis L., roots of Rosa majalis Herrm., Rosa canina L., Arctium lappa L., Arctium minus (Hill) Bernh., Arctium tomentosum Mill., and the herb of Bidens tripartita L.Materials and methods. As study objects the tinctures from rhizomes with roots of Rumex confertus, Sanguisorba officinalis, roots of Rosa majalis, Rosa canina, Arctium lappa, Arctium minus, Arctium tomentosum and the herb of Bidens tripartita were used. These tinctures were obtained by the method of maceration at room temperature and the ratio of 1 : 5 of the plant raw material/finished products; the extractant was 50 % ethyl alcohol. The quantitative content of total polyphenols and the amount of hydroxycinnamic acids was determined by spectrophotometry according to the methods of the State Pharmacopoeia of Ukraine.Results and discussion. The limits of the quantitative content of total polyphenols and the amount of hydroxycinnamic acids in the tinctures were determined. They were not less than 0.070 mg mL-1 and 0.002 mg mL-1 for the tincture of rhizomes with roots of Rumex confertus, 0.100 mg mL-1 and 0.005 mg mL-1 for the tincture of rhizomes with roots of Sanguisorba officinalis, 0.070 mg mL-1 and 0.002 mg mL-1 for the tincture of Rosa majalis roots, 0.080 mg mL-1 and 0.001 mg mL-1 for the tincture of Rosa canina roots, 0.01 mg mL-1 and 0.001 mg mL-1 for the tincture of Arctium lappa roots, 0.010 mg mL-1 and 0.002 mg mL-1 for the tincture of Arctium minus roots, 0.001 mg mL-1 and 0.002 mg mL-1 for the tincture of Arctium tomentosum roots, 0.070 mg mL-1 and 0.001 mg mL-1 for the tincture of Bidens tripartita herb, respectively.Conclusions. The quantitative content of total polyphenols and the amount of hydroxycinnamic acids in tinctures from rhizomes with roots of Rumex confertus, Sanguisorba officinalis, roots of Rosa majalis, Rosa canina, Arctium lappa, Arctium minus, Arctium tomentosum and the herb of Bidens tripartita have been determined. The data obtained will be used in further work on the study of tinctures of these types of the plant raw material.

scholarly journals Potentiometric determination of organic acids in the medicinal plant raw material

2021 ◽  
pp. 11-17
Author(s):  
T. Oproshanska ◽  
O. Khvorost
Keyword(s):  
Organic Acids ◽  
Herbal Medicines ◽  
Raw Material ◽  
Potentiometric Method ◽  
Rosa Canina ◽  
Quantitative Content ◽  
Sanguisorba Officinalis ◽  
First Time ◽  
Arctium Minus

Aim. To analyze the results of the quantitative content of the amount of organic acids in rhizomes with roots of Rumex confertus, rhizomes with roots of Sanguisorba officinalis, roots of Rosa majalis and roots of Rosa canina, roots of Arctium minus and roots of Arctium tomentosum, herb of Bidens tripartite by the potentiometric method, which is available in the monograph “Hibiscus” in the SPhU 2.0. Materials and methods. The study subjects were batches of rhizomes with roots of Rumex confertus, rhizomes with roots of Sanguisorba officinalis, roots of Rosa majalis and roots of Rosa canina, roots of Arctium minus and roots of Arctium tomentosum, herb of Bidens tripartite. The quantitative content of the amount of organic acids was determined by the potentiometric method from the monograph “Hibiscus” SPhU 2.0 using a HI 2550 potentiometer of the “HANNA instruments” company (Germany). Results and discussion. The highest content of the amount of organic acids, among the study subjects was in roots of Arctium minus (from 1.02 ± 0.04 % to 1.05 ± 0.04 %). At the same time, in roots of Arctium tomentosum this amount was almost 3 times lower (from 0.36 ± 0.01 % to 0.37 ± 0.01 %). In the batches of roots of Rosa majalis the content of this group of compounds was almost 2 times higher than in roots of Rosa canina. In rhizomes with roots of Rumex confertus and rhizomes with roots of Sanguisorba officinalis the content of the amount of organic acids differed insignificantly. In herb of Bidens tripartite the content of this group of compounds was 0.78 ± 0.03  – 0.79 ± 0.03 %. In different batches of one type of the raw material the quantitative content of the amount of organic acids varied slightly. Conclusions. For the first time, the quantitative content of the amount of organic acids has been determined in the batches of rhizomes with roots of Rumex confertus, rhizomes with roots of Sanguisorba officinalis, roots of Rosa majalis and roots of Rosa canina, roots of Arctium minus and roots of Arctium tomentosum, herb of Bidens tripartite using the potentiometric method which is available in the monograph “Hibiscus” in the SPhU 2.0. It has been found that the highest content of the amount of organic acids is observed in roots of Arctium minus (not less than 1.02 %) and herb of Bidens tripartite (not less than 0.78 %). The results obtained will be used in further research when creating new herbal medicines with the anti-inflammatory and antimicrobial activity.

scholarly journals Comparative pharmakognostical study of roots of Rosa Majalis Herrm. and Rosa Canina L.

2021 ◽  
pp. 71-78
Author(s):  
Tetiana Oproshanska ◽  
Olga Khvorost ◽  
Kateryna Skrebtsova ◽  
Konradas Vitkevicius
Keyword(s):  
Cell Walls ◽  
Raw Materials ◽  
Total Polyphenols ◽  
Diagnostic Features ◽  
Rosa Canina ◽  
Plant Raw Materials ◽  
Quantitative Content ◽  
Microscopic Features ◽  
Boundary Limits

The aim is to conduct a comparative pharmacognostical study of the series of roots of Rosa (R.) majalis Herrm. and Rosa (R.) canina L. with the establishment of diagnostic features of morphological and anatomical structure and boundary limits of numerical indicators of raw materials. Materials and methods. The fresh and dry raw materials were used to study the macroscopic microscopic features by microscope Delta optical BioLight 300 (Poland). Determination of total polyphenols was performed by spectrophotometry (on a spectrophotometer Optizen POP (Korea)) and HPLC (chromatograph an Agilent 1200 3 D LC System Technologies (USA)). Results. The morphological (nature of the surface (periderm) and fracture) and anatomical (color of cell walls and their cavities; location of the sclerenchyma; the presence of a crystalline coating of the sclerenchyma at the root of R. canina; of various elements of the remains of the tetraarchic conducting bundle in the center of the root) diagnostical features of roots of R. majalis and R. Canina were established. Comparing the numerical values of loss on drying (not more than 10 %), total ash (not more than 5 %), extractable matter (not less than 9 %) and the quantitative content of total polyphenols (not less than 4 %) it was determined that both types of raw materials according to these indicators are almost indistinguishable. Conclusions. Loss on drying, total ash, extractable matter and content of total polyphenols of the root of R. majalis and R. canina do not have significant differences, that is why the root of both plant species can be used as medicinal plant raw materials such as “Rose root”. The obtained data will be used in further research when creating methods of quality control of plant raw materials and phytomedicines

scholarly journals Pharmacognostic analysis of Salvia hispanica L. seeds

2021 ◽  
pp. 49-54
Author(s):  
Anastasiia Sytryn ◽  
Iryna Cholak ◽  
Oksana Yemelianova ◽  
Uliana Karpiuk
Keyword(s):  
Fatty Acids ◽  
Chemical Reactions ◽  
Hydroxycinnamic Acids ◽  
Water Soluble ◽  
Raw Material ◽  
Salvia Hispanica ◽  
Phytochemical Study ◽  
Quantitative Content ◽  
Salvia Hispanica L ◽  
Material Swelling

The aim. The aim of this work was to conduct a microscopic and phytochemical study of the seeds of chia (Salvia hispanica L.). Materials and methods. Chia seeds were examined macroscopically and microscopically. To study the qualitative composition of the main groups of biologically active substances, histochemical, microchemical and chemical reactions were used. Hydroxycinnamic acids were identified by paper chromatography. To obtain a lipophilic extract, a Soxhlet apparatus and an exhaustive chloroform extraction method were used. The study of the quantitative content of fatty acids was carried out by gas chromatography. The content of polysaccharides in the raw material was determined by the gravimetric method. According to the SPhU method, the raw material swelling index was determined. Results. The main macro- and microscopic features of chia seeds have been established. Histochemical reactions, microchemical reactions made it possible to establish the presence of mucus and fatty oils in chia seeds. With the help of chemical reactions, the presence of flavonoids in the raw material was established. The quantitative content of fatty oils is 24.0±1.2 %. The content of water-soluble polysaccharides in the whole raw material was 4.01±0.07 %, in the crushed raw material - 5.04 ± 0.05 %. As a result of determining the swelling index, it was found that this indicator for the whole chia seeds was 20, and for the crushed ones – 17. The content of hydroxycinnamic acids in the chia seeds was 1.07±0.03 %. 9 fatty acids have been identified, among which linoleic acid predominates in terms of content. Conclusions. The presence and quantitative content of mucus, fatty oils, water-soluble polysaccharides, flavonoids, hydroxycinnamic acids, fatty acids was confirmed in the seeds of chia (Salvia hispanica L.). The obtained data can be used to develop regulatory documentation for chia seeds in order to use this raw material in pharmacy and medicine

scholarly journals Parameters of obtaining tincture from underground organs of Sanguisorba officinalisand study its biological activity

2021 ◽  
Vol 14 (3) ◽  
pp. 299-305
Author(s):  
T. V. Oproshanska ◽  
O. P. Khvorost
Keyword(s):  
Antimicrobial Activity ◽  
Antioxidant Activity ◽  
Growth Retardation ◽  
Raw Materials ◽  
Hydroxycinnamic Acids ◽  
Raw Material ◽  
Optimal Parameters ◽  
Antimicrobial And Antioxidant Activity ◽  
Sanguisorba Officinalis

The aim of the work is to experimentally determine the optimal parameters for obtaining a tincture of rhizomes with roots of Sanguisorba officinalis and to investigate its antimicrobial and antioxidant activity. Materials and methods. Rhizomes with roots of Sanguisorba officinalis were harvested in autumn 2019. When establishing the optimal parameters of the technology of obtaining tincture, the evaluation criterion was the number of the sum of polyphenols and the amount of hydroxycinnamic acids, which were determined by spectrophotometric method according to the methods of the State Pharmacopoeia of Ukraine 2.0. Antimicrobial activity was studied by agar diffusion and serial dilutions, antioxidant – in vitro using a stable radical 2,2-diphenyl-1-picrylhydrazyl (2,2-diphenyl-1-picrylhydrazyl – DPPH). Results. The optimal parameters for obtaining a tincture of rhizomes with roots of Sanguisorba officinalis was extraction method maceration at room temperature, raw material-finished product ratio 1:5, time 48 hours, extractant 50 % ethyl alcohol, the number of polyphenols, and the number of hydroxycinnamic acids in terms of dry raw materials not less than 4.0 % and 1.5 % respectively. The resulting tincture exhibits antimicrobial activity against microorganisms Bacillus subtilis ATCC 6633 (growth retardation 26.40 ± 1.04 mm), Staphylococcus aureus ATCC 25923 (growth retardation 24.60 ± 0.68 mm) and Pseudomonas aeruginosa ATCС 27853 (growth retardation 23.60 ± 0.68 mm) and at a dose of 0.02 ml showed antioxidant activity at the level of 70 %. Conclusions. The parameters of obtaining a tincture of rhizomes with roots of Sanguisorba officinalis were determined and its antimicrobial and antioxidant activity was studied.

scholarly journals Development of the method for standardization of the medicinal plant raw material of Cichorium intybus L. herb by the total amount of hydroxycinnamic acid derivatives

Pharmacia ◽  
2021 ◽  
Vol 68 (1) ◽  
pp. 167-173
Author(s):  
Kseniya Proskurina ◽  
Olga Yevtifieieva ◽  
Olga Mala ◽  
Viktoriya Mashtaler
Keyword(s):  
Medicinal Plant ◽  
Quantitative Determination ◽  
Chlorogenic Acid ◽  
Hydroxycinnamic Acids ◽  
Raw Material ◽  
Hydroxycinnamic Acid Derivatives ◽  
Plant Raw Material ◽  
Ethanol Extracts ◽  
Direct Spectrophotometry

The purpose of the research is to work out a routine method for the quantitative determination of the total amount of hydroxycinnamic acids in chicory herb growing in Ukraine. The presence of chlorogenic and caffeic acids was experimentally proven by comparing the TLC profiles of solutions of the chicory extract and standard substances of hydroxycinnamic acids. According to the chromatogram, the chemical composition of the ethanol extracts was qualitatively constant and did not depend on the series of plants collected in different places (7 regions of Ukraine) and in different years (2017 and 2018). The water-alcohol solutions of 8 chicory herb samples had the absorption maximum at the wavelength range from 326 to 330 nm, which was typical for phenolic acids. Chlorogenic acid was chosen as a marker substance for the quantitative determination by direct spectrophotometry. The value of the specific absorbance (556.21(λ=327 nm)) of chlorogenic acid in the concentration of 10.08 µg/ml in ethanol 50% was determined. The total amount of hydroxycinnamic acids was found to be 1.87% for the sample of 2017, for the samples of 2018 in the range of 4.00–6.61%. The method proposed is acceptable for standardization of the medicinal plant raw material.

FLY ASH FROM THERMAL POWER PLANT, RAW MATERIAL FOR GLASS-CERAMIC

2013 ◽  
Vol 12 (2) ◽  
pp. 337-342 ◽  
Author(s):  
Firuta Goga ◽  
Roxana Dudric ◽  
Calin Cormos ◽  
Florica Imre ◽  
Liliana Bizo ◽  
...  
Keyword(s):  
Fly Ash ◽  
Power Plant ◽  
Glass Ceramic ◽  
Thermal Power Plant ◽  
Thermal Power ◽  
Raw Material ◽  
Plant Raw Material

Organic fertilizer from restored plant raw material

2019 ◽  
Vol 97 (8) ◽  
pp. 5-10
Author(s):  
Je. Skryl'nyk ◽  
A. Kutova ◽  
V. Getmanenko ◽  
G. Tsygichko
Keyword(s):  
Organic Fertilizer ◽  
Raw Material ◽  
Plant Raw Material

scholarly journals The Effect of Ripening Stages on the Accumulation of Carotenoids, Polyphenols and Vitamin C in Rosehip Species/Cultivars

2021 ◽  
Vol 11 (15) ◽  
pp. 6761
Author(s):  
Brigita Medveckienė ◽  
Jurgita Kulaitienė ◽  
Dovilė Levickienė ◽  
Ewelina Hallmann
Keyword(s):  
High Performance Liquid Chromatography ◽  
Vitamin C ◽  
High Performance ◽  
Hplc Method ◽  
Total Carotenoid ◽  
Ripening Stage ◽  
Rosa Rugosa ◽  
Total Polyphenols ◽  
Rosa Canina ◽  
Ripening Stages

Our research was aimed at assessing the effect of accumulation of carotenoids, polyphenols, vitamin C and ripening stage in the rosehip fruits of two species—Rosa canina, Rosa rugosa and two cultivar—Rosa rugosa ‘Rubra’ and Rosa rugosa ‘Alba’. The amounts of carotenoids, polyphenols and vitamin C were determined using the high-performance liquid chromatography (HPLC) method. The obtained results showed that the significantly highest amount (107.15 mg 100 g−1) of total carotenoid was determined in the fruits of Rosa canina at ripening Stage V. While results indicated that significant amount of total polyphenols were established at Stages I and II in the Rosa Rugosa ‘Alba’ and Rosa rugosa ‘Rubra’ cultivars (110.34 mg 100 g−1, 107.88 mg 100 g−1 and 103.20 mg 100 g−1 103.39 mg 100 g−1). At ripening Stage I, in the fruits of Rosa rugosa the greatest increases were established in the contents of vitamin C (3036.08 mg 100 g−1).

TECHNOLOGY FOR OBTAINING PECTIN CONCENTRATE FROM ROSEHIP FRUITS

2021 ◽  
pp. 87-90
Author(s):  
М.Ж. Кизатова ◽  
Д.А. Абдуллаева
Keyword(s):  
Medicinal Plant ◽  
Raw Materials ◽  
Raw Material ◽  
Plant Raw Materials ◽  
Plant Raw Material ◽  
Pectin Substances

Статья посвящена важности пектиновых веществ, а также расширению сферы использования лекарственного растительного сырья. В качестве лекарственного растительного сырья представлены технологии получения пектинового экстракта из плодов шиповника, которые встречаются на территории Казахстана 25 видов. Применение пектина в медицине. The article is devoted to the importance of pectin substances, as well as to the expansion of the use of medicinal plant raw materials. As a medicinal plant raw material, technologies for obtaining pectin extract from rosehip fruits, which are found in 25 species on the territory of Kazakhstan, are presented. The use of pectin in medicine

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