Knowledge and Practices Assessment of Decontamination during Root Canal Treatment in Saudi Arabia

Introduction: Bacteria play a crucial role in the pathogenesis of pulpal and periradicular pathoses. For example: Apical periodontitis is an inflammation of dental periapical tissues developed as a response to colonization of microorganisms in root canal system. The elimination of microbial species from the infected root canal system necessitates a strict aseptic condition for Instrumentation, disinfection and inter appointment medication. Enterococcus faecalis and Candida albicans had been commonly associated organisms in treatment-resistant infections. Methods: This was an observational cross-sectional study based on web-survey questionnaires developed by the authors with high confidence. The sample size was 304 estimated using the Qualtrics calculator with a confidence level of 95% and a margin of error of 5%. The questionnaire is divided into sections, regarding endodontic practice include endodontic education , years of experience , used of rubber dam or no and how to used irrigant solution whoever Sodium hypochlorite (NaOCl), Chlorhexidine, chelating agents (EDTA) or (MTAD) and reasons of every irrigant that prevents during root canal treatment. Data were collected using Google forms and prepared for analysis using Microsoft Excel. Statistical analysis was performed using the SPSS software. Results: A total of 304 participants responded, 44.1% dental interns, 39.5% General Dentist, 9.9% Endodontist, 6.3% other dental specialty. the Probable reasons that prevent them using rubber dam during root canal treatment are: 5.9% due to difficulty to apply, 9.2% due to lack of materials, 5.3% because it is a time-consuming, 1.6% said that other isolation methods are enough (partial isolation), 3.9% because of multiple reason combined together. 48.7% they always use rubber dam, so they did not choose any of the previous reasons. Conclusion: In conclusion, we identify and understand, most of the participants “always using rubber dam” during root canal treatment and the most common reasons was time consumption that prevented dentist from using rubber. In this study majority of participants preferred NaOCl 81.9% and 7.1% don’t use it.

Topical Exposure to Nemopilema nomurai Venom Triggers Oedematogenic Effects: Enzymatic Contribution and Identification of Venom Metalloproteinase

Scyphozoan envenomation is featured as severe cutaneous damages due to the toxic effects of venom components released by the stinging nematocysts of a scyphozoan. However, the oedematogenic property and mechanism of scyphozoan venoms remain uninvestigated. Here, we present the oedematogenic properties of the nematocyst venom from Nemopilema nomurai (NnNV), a giant stinging scyphozoan in China, for the first time, using in vivo and in vitro models with class-specific inhibitors. NnNV was able to induce remarkable oedematogenic effects, including induction of significant oedema in the footpad and thigh of mouse, and increase in vascular permeability in the dorsal skin and kidney. Moreover, batimastat, a specific metalloproteinase inhibitor, could significantly reduce the Evan’s blue leakage in the damaged organs and attenuate paw oedema after 12 h, but exerted no influence on NnNV-induced thigh oedema. These observations suggested a considerable contribution of NnNV metalloproteinase-like components to the increased vasopermeability, and the participation was strongly suggested to be mediated by destroying the integrity of the vascular basement membrane. Moreover, partial isolation combined LC-MS/MS profiling led to identification of the protein species Nn65 with remarkable metalloproteinase activity. This study contributes to the understanding of the effector components underlying the cutaneous damages induced by scyphozoan stings.

Hybrid Incompatibilities and Transgressive Gene Expression Between Two Closely Related Subspecies of Drosophila

Genome-wide assays of expression between species and their hybrids have identified genes that become either over- or underexpressed relative to the parental species (i.e., transgressive). Transgressive expression in hybrids is of interest because it highlights possible changes in gene regulation linked to hybrid dysfunction. Previous studies in Drosophila that used long-diverged species pairs with complete or nearly complete isolation (i.e., full sterility and partial inviability of hybrids) and high-levels of genome misregulation have found correlations between expression and coding sequence divergence. The work highlighted the possible effects of directional selection driving sequence divergence and transgressive expression. Whether the same is true for taxa at early stages of divergence that have only achieved partial isolation remains untested. Here, we reanalyze previously published genome expression data and available genome sequence reads from a pair of partially isolated subspecies of Drosophila to compare expression and sequence divergence. We find a significant correlation in rates of expression and sequence evolution, but no support for directional selection driving transgressive expression in hybrids. We find that most transgressive genes in hybrids show no differential expression between parental subspecies and used SNP data to explore the role of stabilizing selection through compensatory mutations. We also examine possible misregulation through cascade effects that could be driven by interacting gene networks or co-option of off-target cis-regulatory elements.

Partial Isolation Type Buried Channel Array Transistor (Pi-BCAT) for a Sub-20 nm DRAM Cell Transistor

In this paper, we propose a new buried channel array transistor structure to solve the problem of current leakage occurring in the capacitors of dynamic random-access memory (DRAM) cells. This structure has a superior off current performance compared with three previous types of structures. In particular, the proposed buried channel array transistor has a 43% lower off current than the conventional asymmetric doping structure. Here, we show the range of the effective buried insulator parameter according to the depth of the buried gate, and we effectively show the range of improvement for the off current.

e EFFECT OF ?-Al2O3 NANOPARTICLES ADDITION ON SOME PROPERTIES OF CoCrMo ALLOY FABRICATED BY POWDER METALLURGY ROUTE

CoCrMo alloys are used as biomaterial due to their good corrosion and wear resistance, with reasonable biocompatibility. The present study aims to investigate the effect of (1, 3 and 5) wt% ?-Al2O3 nanoparticle additions on some properties of pre-alloyed CoCrMo when fabricated by powder metallurgy route. The density, porosity, microhardness and microstructure of the fabricated samples were examined. The results revealed that the addition of ?-Al2O3 nanoparticle has a distinguished effect on the density of sintered CoCrMo alloy samples, where continues increase in the porosity was happened and reached to its maximum value at the higher addition amount. While the density showed an inverse trend to that of the porosity with the increasing of the nanoparticle addition. The microhardness was decreased as the amount of the nanoparticle addition to CoCrMo alloy was increased. The microstructure observation by SEM and SEM-Mapping is revealed the partial isolation of CoCrMo particles by the nanoparticle additions and it was associated with the formation of passive Cr2O3 oxide. In turn the XRD analysis depicted the co-existence of ?-Co and ?-Co phases besides, the Cr2O3 oxide. Although the increased porosity with the nanoparticle addition but, this will perhaps, increase the tissue ingrowth when the fabricated nanocomposites were utilized as biomaterial

Inferring Effective Population Size and Divergence Time in the Lithuanian Population According to High-Density Genotyping Data

The prehistory of the Lithuanian population and genetic relationship to other populations are poorly studied. Thus, the Lithuanian population, as an object of study, is interesting due to its partial isolation with genetic distinctiveness within the European context and with preserved ancient genetic composition. The main objects of this study was to infer demographic parameters, effective population size (Ne), and divergence time using high-density single nucleotide polymorphism (SNP) genotyping data generated with the Illumina HumanOmmiExpress-12v1.1 array in 295 individuals from the Lithuanian population and to compare our data with other populations from the Human Genome Cell Line Diversity Panel (HGDP-CEPH). We also aimed to reconstruct past events between the main ethnolinguistic regions—Aukštaitija and Žemaitija of Lithuania. Historically, these regions probably developed as two independent Baltic tribes. Our results of Ne in the Lithuanian population through time demonstrated a substantial reduction of Ne over the 150,000–25,000 years before present (YBP). The estimated long-term Ne of the Lithuanian population is quite low—it equals 5404, which likely is a consequence of the bottlenecks associated with the last glacial period of 25,000–12,000 YBP in Europe. The obtained divergence time estimates between the study populations are in agreement with recent studies. The reconstructed past events in Aukštaitija and Žemaitija showed significant differences between these two regions of Lithuania.

Heritage Language and Cultural Preservation among the Nomadic Turkmen of Jordan: A Sociolinguistic Study

This study aimed to explore how the nomadic Turkmen of Jordan use Turkmen and Arabic in their daily life. It also describes the cultural aspects that they preserved and the factors that helped them in preserving their language and culture. Adopting the case study approach, a five-Turkmen-Family sample served as participants utilising the participants’ observation method and semi-structured interviews. Results revealed that the nomadic Turkmen of Jordan have maintained their ethnic language by using it exclusively at home with family members, grandparents and with relatives. They also preserved various aspects of their heritage culture such as clothing, life style, marriage and social traditions as well as their eating habits. Moreover, results have revealed some socio-cultural factors that enhanced their language and cultural preservation such as partial isolation from the larger host community, rejection of external marriages, lack of education and the media.