Time course of recovery following CrossFit® Karen Benchmark Workout in trained men

The study describes the acute and delayed time course of recovery following the CrossFit® Benchmark Workout Karen. Eight trained men (28.4±6.4 years; 1RM back squat 139.1±26.0 kg) undertook the Karen protocol. The protocol consists of 150 Wall Balls, aiming to hit a target 3 meters high. Countermovement jump height (CMJ), creatine kinase (CK), and perceived recovery status scale (PRS) (general, lower and upper limbs) were assessed pre, post-0h, 24h, 48h and 72h after the session. The CK concentration 24h after was higher than pre-exercise (338.4 U/L vs. 143.3 U/L; effect size: 0.74; p≤0.05). At 48h and 72h following exercise, CK concentration had returned to baseline levels. The PRS general and of the lower limbs were lower in the 24-hours post-exercise compared to pre-exercise (PRS general: 4.7 ±1.5 and 7.9 ±1.7 mmol/L; and PRS of the lower limbs: 4.0 ±2.5 and 7.9 ±0.8, respectively). The PRS general, lower, and upper limbs were reduced at 48-post exercise compared to 72-hours post-exercise scores. Our findings provide insights into the fatigue profile and recovery in acute CrossFit® and can be useful to coaches effectively design the daily session.

Elimination of three doses of gentamicin over three consecutive days using a polyacrylonitrile-derived filter: An in vitro assessment

Introduction: Adsorption of gentamicin in a polyacrylonitrile filter was previously evidenced in a session lasting 6 h using the NeckEpur model. We extended the study over three consecutive days to mimic the 72-h life span of a filter. Methods: Prismaflex® monitor and ST150® filter were used in the continuous diafiltration (CDF) mode at a 2.5 L/h flowrate. The daily session started with a 6-h session of CDF. Thereafter, the 5-L central compartment was changed using a bag free of gentamicin to assess gentamicin release over the following 18 h. Experiments were repeated on Day 2 and stopped at the end of the 6-h session of CDF on Day 3. The experiment was performed in duplicate. Results: At a 2.5 L/h diafiltration flowrate, the mean daily clearances of gentamicin were 5.5, 4.0, and 3.3 L/h, respectively. The mean diafiltration and adsorption ratios in the daily elimination of gentamicin were 32/68%, 58/42%, and 88/12%, respectively. During days 1 and 2, the mean amount of gentamicin released from the ST150® filter were 14 and 34 mg, respectively. Conclusion: The pharmacokinetics of gentamicin over 3 days is strongly altered by adsorption in the same filter with a progressive decrease of elimination by adsorption, suggesting saturation of the filter. One limitation of our study results from the mode of administration using a bolus dose instead of an infusion over 30 min. Adsorption adds a clearance to those of diafiltration. The time-dependency of gentamicin clearance precludes using a constant dosage regimen over the filter’s life span.

Daily Session Schedule

This chapter presents how all the pieces of the Prolonged Exposure-Intensive Outpatient Program (PE-IOP) fit together to form a program. The authors discuss areas for variations and ways to improve efficiency. Each day of the PE-IOP program is described, with each component noted and the detailed specifics outlined. Suggested time markers for sessions are presented.

Desarrollo de las capacidades coordinativas en niños: efectos de entrenamiento en el patinaje (Development of coordination skills in children: effects of rollerblading training)

El objetivo del estudio fue determinar el efecto de un programa de aprendizaje de las capacidades coordinativas sobre el tiempo en la prueba de patinaje de 300 metros contra reloj individual y la coordinación general. Participaron 14 niños y niñas entre los seis y ocho años de edad que fueron asignados aleatoriamente al grupo experimental (n=7) o grupo control (n=7); la media de la edad del grupo experimental fue 8,1 años (DE=0.5) y la media del grupo control 7,6 años (DE=0.82); con una experiencia deportiva igual para ambos grupos (16 meses). El grupo experimental tuvo un entrenamiento adicional de coordinación antes de la sesión cotidiana durante 20 sesiones distibuidas en siete semanas. El programa constó de 15 ejercicios en escalerilla realizados en cada sesión asi: tres ejercicios con dos series de 10 repeticiones y recuperación de 30 segundos en la primera serie y dos minutos después de la segunda. El grupo control no recibió estímulo adicional y continuó con el entrenamiento cotidiano del patinaje. Se realizó pre y post-test donde se midió la velocidad con la prueba de 300 m contra reloj individual en patines y la coordinación de los niños con el test Korperkoordination Test fur Kinder. Los resultados mostraron que existen diferencias significativas en las comparaciones intra-grupo, sin embargo, al realizar la comparación inter-grupos, se constató que no existen diferencias significativas (p= 0.180) en velocidad, pero en la práctica del patinaje estas diferencias son relevantes porque determinan una disminución en el tiempo de una prueba contra reloj, que puede significar un cambió ecológico significativo.Abstract. The research objective was to determine the effect of a learning program for coordination skills on the time of the 300-meter rollerblading test against individual clock and on general coordination. A sample of 14 boys and girls between six and eight years old, who were randomly assigned to experimental group (n=7) or control group (n=7) participated in the study; average age of the experimental group was 8,1 years old (SD=0,5), whereas in the control group it was 7,6 years old (SD=0.82); participants from both groups had similar sports experience (16 months). During 20 sessions over seven weeks, the experimental group received additional coordination training before each daily session. The program consisted of 15 ladder exercises, where in each session three exercises were performed with two sets of 10 repetitions and recovery of 30 seconds in the first series and two minutes after the second. The control group did not receive additional stimulus and continued with daily rollerblading training. Pre- and post-test were performed, with speed being measured during the 300m test against individual clock, whilst children's coordination was assessed with the Korperkoordination Test fur Kinder Test. The results showed significant differences in intra-group comparisons; however, when performing the inter-group comparison, no significant differences (p = 0.180) were found in speed. Nonetheless, in rollerblading practice these differences are relevant, because they determine a decrease in the time of a test against the clock, which may mean a significant ecological change.

Exposure pattern influences the degree of drug-seeking behaviour after withdrawal

ObjectivesThe occurrence of a relapse during abstinence is an important issue that must be addressed during treatment for drug addiction. We investigated the influence of drug exposure pattern on morphine-seeking behaviour following withdrawal. We also studied the role of the hippocampus in this process to confirm its involvement in drug relapse.MethodsMale Sprague–Dawley rats that were trained to self-administer morphine (1.0 mg/kg) using 2, 4, 6, 8, or 10 h daily sessions underwent withdrawal in their home cages and were re-exposed to the operant chamber to evaluate morphine-seeking behaviour. During the relapse session, rats were intravenously injected with morphine (0.25 mg/kg) or saline before re-exposure to the chamber. In the second experiment, rats were administered a microinjection of saline or cobalt chloride (CoCl2, 1 mM), a synaptic blocker, into the CA1 of the hippocampus prior to the relapse test.ResultsIn the first experiment, more morphine-seeking behaviour was observed in the 2 h group (animals trained to self-administer morphine during a 2 h daily session spread over 21 days) during the relapse session, despite all groups being exposed to similar amounts of morphine during the training period before withdrawal. In the second experiment, pretreatment with CoCl2 markedly reduced morphine-seeking behaviour in the 2 h group.ConclusionsThe present findings suggest that the exposure pattern influences the degree of relapse and that control of memorisation is important for prevention of relapse.

Reward comparison in chronic decerebrate rats

The simultaneous contrast paradigm was used to evaluate responsiveness to a low (0.05 M) and a high (0.5 M) concentration of sucrose under two conditions in intact and chronic decerebrate rats. In one condition the low concentration was presented on one day and the high concentration on another. In the other condition presentation of the two concentration was alternated within the same daily session. In each case there was a total of 40 trials/day during which the stimulus was delivered intraorally for 2 s at a rate of 1.5 ml/min with a 30-s intertrial interval. The results showed that the intact rats always licked more for the high than for the low concentration of sucrose but that the magnitude of the effect was larger when given the opportunity to compare the two concentrations within the same daily session. The decerebrate rats produced a similar pattern, but the concentration effect was evident only when the stimuli were alternated within the same daily session. These data stand as the first evidence that the isolated caudal brain stem is adequate for the expression of a behavior that depends on comparison processes involving short-term memory.