Wetting of the tarsal adhesive fluid determines underwater adhesion in ladybug beetles

Many insects can climb smooth surfaces using hairy adhesive pads on their legs mediated by tarsal fluid secretions. It was previously shown that a terrestrial beetle can even adhere and walk underwater. The naturally hydrophobic hairs trap an air bubble around the pads, allowing the hairs to make contact to the substrate like in air. However, it remained unclear to what extent such an air bubble is necessary for underwater adhesion. To investigate the role of the bubble, we measured the adhesive forces inindividual legs of live but constrained ladybug beetles underwater in the presence and absence of a trapped bubble and compared it with its adhesion in air. Our experiments revealed that on a hydrophobic substrate, even without a bubble, the pads show adhesion comparable to that in air. On a hydrophilic substrate, underwater adhesion is significantly reduced, with or without a trapped bubble. We modelled the adhesion of a hairy pad using capillary forces. Coherent with our experiments, the model demonstrates that the wetting properties of the tarsal fluid alone can determine the ladybugs’ adhesion to smooth surfaces in both air and underwater conditions and that an air bubble is not a prerequisite for their underwater adhesion. The study highlights how such a mediating fluid can serve as a potential strategy to achieve underwater adhesion via capillary forces, which could inspire artificial adhesives for underwater applications.

Multi-Technique Investigation of a Biomimetic Insect Tarsal Adhesive Fluid

There is substantial motivation to develop novel adhesives which take advantage of the superior adhesive strength and adaptability of many natural animal adhesives; however, the tools typically used to study these mechanisms are incapable of determining the precise interactions of molecules at an adhesive interface. In this study, a surface specific, order sensitive vibrational spectroscopy called sum frequency generation (SFG) is, for the first time, combined with multiple bulk characterization techniques to examine a novel, simple biomimetic adhesive fluid inspired by tarsal fluid of insects. Insects perform complex adhesive demands, including sticking, climbing vertically and running upside-down with little difficulty. Thus, we hypothesize that both bulk and surface specific properties of the fluid contribute to the success of this wet adhesive mechanism. SFG spectra of biomimetic emulsion exhibited similar hydrocarbon organization on hydrophobic and hydrophilic substrates to natural beetle fluid previously studied with the same method. Bulk characterization techniques indicated that the emulsion had a shear-thinning profile with the ability to enhance traction forces during climbing and low surface tension ideal for surface wetting on the majority of natural surfaces. Multi-technique comparisons between emulsion and pure squalane revealed that a hydrocarbon only based fluid could not replicate the traction promoting properties of the emulsion. We conclude that the insect tarsal fluid adhesive mechanism relies upon contributions from both surface-specific properties optimizing traction force and bulk properties promoting rapid surface wetting and maintaining pull-off force for fast detachment.

Attachment devices and the tarsal gland of the bug Coreus marginatus (Hemiptera: Coreidae)

The present ultrastructural investigation using scanning and transmission electron microscopy as well as light and fluorescence microscopy describes in detail the attachment devices and tarsal gland of the bug Coreus marginatus (L.) (Hemiptera: Coreidae). In particular, the fine structure of pulvilli reveals a ventral surface rich with pore channels, consistent with fluid emission, and a folded dorsal surface, which could be useful to enhance the pulvillus contact area during attachment to the substrate. The detailed description of the tarsal gland cells, whose structure is coherent with an active secretory function, allows us to consider the tarsal gland as the plausible candidate for the adhesive fluid production. Scolopidia strictly adhering to the gland cells are also described. On the basis of the fine structure of the tarsal gland, we hypothesise a fluid emission mechanism based on changes of the hydraulic pressure inside the gland, due to the unguitractor tendon movements. This mechanism could provide the fluid release based on compression of the pad and capillary suction, as demonstrated in other insects. The data here reported can contribute to understanding of insect adhesive fluid production, emission and control of its transport.

Mathematical fractional modeling of transpot phenomena of viscous fluid-flow between two plates

This work is about the mass and heat transfer flow for adhesive fluid between two upright plates pulled apart by a distance, d. Fractional model of the considered problem is developed after making governing equations dimensionless. Laplace transform technique is utilized to acquire analytical solutions and some graphics are presented to see the physical behavior of embedded parameters.

Influence of ambient humidity on the attachment ability of ladybird beetles (Coccinella septempunctata)

Many insects possess adhesive foot pads, which enable them to scale smooth vertical surfaces. The function of these organs may be highly affected by environmental conditions. Ladybird beetles (Coccinellidae) possess dense tarsal soles of tenent setae, supplemented with an adhesive fluid. We studied the attachment ability of the seven-spotted ladybird beetle (Coccinella septempunctata) at different humidities by horizontal traction experiments. We found that both low (15%) and high (99%) relative humidities lead to a decrease of attachment ability. The significantly highest attachment forces were revealed at 60% humidity. This relationship was found both in female and male beetles, despite of a deviating structure of adhesive setae and a significant difference in forces between sexes. These findings demonstrate that not only dry adhesive setae are affected by ambient humidity, but also setae that stick due to the capillarity of an oily secretion.

Suitability of carbon fiber–reinforced polyetheretherketone cages for use as anterior struts following corpectomy

OBJECTIVE Fibular allograft remains a widely used strut for corpectomy surgeries. The amount of graft material that can be packed into an allograft strut has not been quantified. Cages are an alternative to fibular allograft for fusion surgeries. The authors of this study assessed the suitability of carbon fiber–reinforced polyetheretherketone (CFRP) cages for anterior corpectomy surgeries. They further explored the parameters known to affect fusion rates in clinical practice. METHODS Six fibular allografts were tested at standard lengths. Three sets of carbon fiber cages (Bengal, DePuy Spine), each with a different footprint size but the same lengths, were tested. The allografts and cages were wrapped in adhesive, fluid-tight transparent barriers and filled with oil. The volume and weight of the oil instilled as well as the implant footprints were measured. The fibular allografts and cages were tested at 20-, 40-, and 50-mm lengths. Two investigators independently performed all measurements 5 times. Five CFRP cubes (1 × 1 × 1 cm) were tested under pure compression, and load versus displacement curves were plotted to determine the modulus of elasticity. RESULTS Significantly more oil fit in the CFRP cages than in the fibular allografts (p < 0.0001). The weight and volume of oil was 4–6 times greater in the cages. Interobserver (r = 0.991) and intraobserver (r = 0.993) reliability was excellent. The modulus of elasticity for CFRP was 16.44 ± 2.07 GPa. CONCLUSIONS Carbon fiber–reinforced polyetheretherketone cages can accommodate much more graft material than can fibular allografts. In clinical practice, the ability to deliver greater amounts of graft material following a corpectomy may improve fusion rates.

Physical principles of fluid-mediated insect attachment - Shouldn’t insects slip?

Insects use either hairy or smooth adhesive pads to safely adhere to various kinds of surfaces. Although the two types of adhesive pads are morphologically different, they both form contact with the substrate via a thin layer of adhesive fluid. To model adhesion and friction forces generated by insect footpads often a simple “wet adhesion” model is used, in which two flat undeformable substrates are separated by a continuous layer of fluid. This review summarizes the key physical and tribological principles that determine the adhesion and friction in such a model. Interestingly, such a simple wet-adhesion model falls short in explaining several features of insect adhesion. For example, it cannot predict the observed high static friction forces of the insects, which enable them to cling to vertical smooth substrates without sliding. When taking a closer look at the “classic” attachment model, one can see that it is based on several simplifications, such as rigid surfaces or continuous layers of Newtonian fluids. Recent experiments show that these assumptions are not valid in many cases of insect adhesion. Future tribological models for insect adhesion thus need to incorporate deformable adhesive pads, non-Newtonian properties of the adhesive fluid and/or partially “dry” or solid-like contact between the pad and the substrate.

The Design and Modeling of Jetting Dispenser Actuated by Dual Piezostack Actuator

This article presents performance results of a novel jetting dispenser system actuated dual piezostack actuators. The proposed piezo jetting dispenser system consists of a couple of piezostack actuators, lever mechanism, and needle part. The proposed dispenser can provide a very small dispensing dot size of high viscous adhesive, 10,000cp at 100°C, at a high dispensing flow rate in semi-conductor packaging processes. After describing the mechanism and operating principle of the proposed dispenser, a mathematical model of the system is obtained by considering behaviors of the piezostack, the return spring, the dispensing needle, and the adhesive fluid dynamics. For the computer simulation, the specific geometric dimensions of the proposed jetting dispenser are chosen in order to achieve operation requirements: needle motion amplitude: up to 0.15 mm; operating frequency: up to 500 Hz. With the high viscosity conditions, the dispensed amount of the adhesive and the maximum displacement of the piezo and the needle at 500Hz are evaluated in time domain.

Some Observations on the Pollination of Round-Leaf Orchid, Galearis (Amerorchis) rotundifolia, Near Jasper, Alberta

On June 16 and 17, 2010, data were collected on pollination in a population of Galearis rotundifolia at the confluence of the Maligne and Athabasca Rivers north of Jasper, Alberta. The primary pollinator was the bee Osmia proxima and this species was also the most frequent visitor. While most flies were visitors, four species, Eriozona (Megasyrphus) laxus, Eristalis (Eoseristalis) hirta, Eristalis (Eoseristalis) rupium, and Eupeodes (Lapposyrphus) lapponicus also served as pollinators. It is estimated that 25-44% or more of flowers were pollinated in the previous year, a relatively high percentage that supports the "advertisement model" for evolution of food deception in orchids. The pollinating bee or fly lands on the lip and probes the spur which is approximately the same length or longer than the tongue. In the process of pushing into the flower and backing out, the sticky contents of a bursicle are either discharged by the backward movement of a flap or by forward pressure. Either of these actions may release adhesive fluid which fixes the viscidia onto the front of the insect's head. The gradual bending forward of the caudicles reduces likelihood of pollination of consecutively visited flowers with pollen from those recently visited flowers on the same plant (geitonogamous pollination) and thus promotes outcrossing. Erratum included.