Kinship effects in quasi-social parasitoids II: co-foundress relatedness and host dangerousness interactively affect host exploitation

Sclerodermus brevicornis is a parasitoid that exhibits cooperative multi-foundress brood production. Prior work showed that the time lag to paralysis of small-sized hosts is shorter when co-foundress relatedness is higher and predicted that the greater risks and greater benefits of attacking larger hosts would combine with co-foundress relatedness to determine the limits to the size of a host that a female is selected to attack as a public good. It was also predicted that the time to host attack would be affected by an interaction between host size and relatedness. Here, we show empirically that both host size and kinship affect S. brevicornis reproduction and that they interact to influence the timing of host attack. We also find effects of co-foundress relatedness after hosts have been suppressed successfully. A public goods model using parameters estimated for S. brevicornis again suggests that selection for individual foundresses to attack and, if successful, to share hosts will be dependent on both the size of the host and the relatedness of the foundresses to any co-foundresses present. Females will not be selected to bear the individual cost of a public good when hosts are large and dangerous or when their relatedness to the co-foundress is low. We conclude that although reproductive behaviours exhibited by Sclerodermus females can be cooperative, they are unlikely to be exhibited without reference to kinship or to the risks involved in attempting to suppress and share large and dangerous hosts.

Social Bacteriophages

Despite their simplicity, viruses can display social-like interactions such as cooperation, communication, and cheating. Focusing on bacteriophages, here we review features including viral product sharing, cooperative evasion of antiviral defenses, prudent host exploitation, superinfection exclusion, and inter-phage peptide-mediated signaling. We argue that, in order to achieve a better understanding of these processes, their mechanisms of action need to be considered in the context of social evolution theory, paying special attention to key population-level factors such as genetic relatedness and spatial structure.

Does the Latent Period of Leaf Fungal Pathogens Reflect Their Trophic Type? A Meta-Analysis of Biotrophs, Hemibiotrophs, and Necrotrophs

We performed a meta-analysis to search for a relation between the trophic type and latent period of fungal pathogens. The pathogen incubation period and the level of resistance of the hosts were also investigated. This ecological knowledge would help us to more efficiently regulate crop epidemics for different types of pathogens. We gathered latent period data from 103 studies dealing with 51 fungal pathogens of the three major trophic types (25 biotrophs, 15 hemibiotrophs, and 11 necrotrophs), representing 2,542 mean latent periods. We show that these three trophic types display significantly different latent periods. Necrotrophs exhibited the shortest latent periods (<100 degree-days [DD]), biotrophs had intermediate ones (between 100 and 200 DD), and hemibiotrophs had the longest latent periods (>200 DD). We argue that this relation between trophic type and latent period points to two opposing host exploitation strategies: necrotrophs mount a rapid destructive attack on the host tissue, whereas biotrophs and hemibiotrophs avoid or delay the damaging phase. We query the definition of hemibiotrophic pathogens and discuss whether the length of the latent period is determined by the physiological limits inherent to each trophic type or by the adaptation of pathogens of different trophic types to the contrasting conditions experienced in their interaction with the host.

Foraging egg parasitoids, Trissolcus vassilievi (Hymenoptera: Platygastridae), respond to host density and conspecific competitors in a patchy laboratory environment

The egg parasitoid Trissolcus vassilievi (Mayr) is a key natural enemy of sunn pest, Eurygaster integriceps Puton (Hemiptera: Scutelleridae). This laboratory study examined how the aggregation of parasitoids varied in response to host density and numbers of foraging conspecifics. Five host densities (1, 2, 4, 8, and 15 clutches of E. integriceps eggs), were offered simultaneously to 1, 2, 4, 8, or 16 female wasps in a standardized arena (30 cm diameter) and patch residence times were recorded. Patch residence time was strongly correlated with parasitism, and increased as the number of host clutches increased up to eight, but declined when more clutches were offered. Wasps displayed low mobility and tended to remain in the patch initially encountered, even when it contained few egg masses. At higher wasp densities, patches were occupied more quickly, host exploitation began sooner, and per capita handling time was reduced. However, total patch residence times were similar across all densities. Thus, females responded to conspecifics with increased movement, which increased dispersal and resulted in a more homogeneous distribution of wasps among patches. Pseudo-interference, resulting from wasps remaining in some patches and neglecting others, had greater impact on final levels of host exploitation than did actual interference (patch abandonment following conspecific encounters). These phenomena resulted in decreased parasitoid searching rates and a 2-fold increase in host survival at higher wasp densities.

Beyond host regulation: Changes in gut microbiome of permissive and non-permissive hosts following parasitization by the wasp Cotesia flavipes

ABSTRACT Koinobiont parasitoids regulate the physiology of their hosts, possibly interfering with the host gut microbiota and ultimately impacting parasitoid development. We used the parasitoid Cotesia flavipes to investigate if the regulation of the host would also affect the host gut microbiota. We also wondered if the effects of parasitization on the gut microbiota would depend on the host–parasitoid association by testing the permissive Diatraea saccharalis and the non-permissive Spodoptera frugiperda hosts. We determined the structure and potential functional contribution of the gut microbiota of the fore-midgut and hindgut of the hosts at different stages of development of the immature parasitoid. The abundance and diversity of operational taxonomic units of the anteromedial (fore-midgut) gut and posterior (hindgut) region from larvae of the analyzed hosts were affected by parasitization. Changes in the gut microbiota induced by parasitization altered the potential functional contribution of the gut microbiota associated with both hosts. Our data also indicated that the mechanism by which C. flavipes interferes with the gut microbiota of the host does not require a host-parasitoid coevolutionary history. Changes observed in the potential contribution of the gut microbiota of parasitized hosts impact the host's nutritional quality, and could favor host exploitation by C. flavipes.

Hooked on you: shape of attachment structures in cymothoid isopods reflects parasitic strategy

Background Parasite attachment structures are critical traits that influence effective host exploitation and survival. Morphology of attachment structures can reinforce host specificity and niche specialisation, or even enable host switching. Therefore, it is important to understand the determinants of variation in attachment structures. Cymothoid isopods are striking ectoparasites of fishes that include the infamous ‘tongue-biters.’ They are known to parasitise hosts in one of four qualitatively distinct anatomical regions. Here, we quantify variation in cymothoid attachment structures — hook-like appendages called dactyli — and test whether differences in dactylus shape are correlated with parasite mode (where they attach), allometry, or both, using multivariate ordinary least squares regression. We also assess the influence of shared ancestry on shape using a molecular phylogeny to weight our models using phylogenetic generalised least squares regression. Results We find clear differences in shape between externally-attaching and internally-attaching cymothoids but also between anterior and posterior dactyli across various species with the same attachment mode. Allometric effects are significant for anterior but not posterior dactyli. Mouth-attaching species show greater shape variability than gill- and mouth-attaching species. We find no evidence that there are clade-specific patterns of association between parasite mode and dactylus shape. Conclusions Parasite mode appears to be the main driver of attachment morphology. This likely reflects several components of parasite ecology including feeding and functional demands of attachment in different microhabitats. Geometric morphometric approaches to the quantification of shape variation of simple structures is an effective tool that provides new insights into the evolvability of parasite attachment.

The role of exploitation in the establishment of mutualistic microbial symbioses

ABSTRACTEvolutionary theory suggests that the conditions required for the establishment of mutualistic symbioses through mutualism alone are highly restrictive, often requiring the evolution of complex stabilising mechanisms. Exploitation, whereby initially the host benefits at the expense of its symbiotic partner and mutual benefits evolve subsequently through trade-offs, offers an arguably simpler route to the establishment of mutualistic symbiosis. In this review, we discuss the theoretical and experimental evidence supporting a role for host exploitation in the establishment and evolution of mutualistic microbial symbioses, including data from both extant and experimentally evolved symbioses. We conclude that exploitation rather than mutualism may often explain the origin of mutualistic microbial symbioses.

Revisiting Bacterial Ubiquitin Ligase Effectors: Weapons for Host Exploitation

Protein ubiquitylation plays a central role in eukaryotic cell physiology. It is involved in several regulatory processes, ranging from protein folding or degradation, subcellular localization of proteins, vesicular trafficking and endocytosis to DNA repair, cell cycle, innate immunity, autophagy, and apoptosis. As such, it is reasonable that pathogens have developed a way to exploit such a crucial system to enhance their virulence against the host. Hence, bacteria have evolved a wide range of effectors capable of mimicking the main players of the eukaryotic ubiquitin system, in particular ubiquitin ligases, by interfering with host physiology. Here, we give an overview of this topic and, in particular, we detail and discuss the mechanisms developed by pathogenic bacteria to hijack the host ubiquitination system for their own benefit.