Giant clam growth in the Gulf of Aqaba is accelerated compared to fossil populations

The health of reef-building corals has declined due to climate change and pollution. However, less is known about whether giant clams, reef-dwelling bivalves with a photosymbiotic partnership similar to that found in reef-building corals, are also threatened by environmental degradation. To compare giant clam health against a prehistoric baseline, we collected fossil and modern Tridacna shells from the Gulf of Aqaba, Northern Red Sea. After calibrating daily/twice-daily growth lines from the outer shell layer, we determined that modern individuals of all three species ( Tridacna maxima , T. squamosa and T. squamosina ) grew faster than Holocene and Pleistocene specimens. Modern specimens also show median shell organic δ 15 N values 4.2‰ lower than fossil specimens, which we propose is most likely due to increased deposition of isotopically light nitrate aerosols in the modern era. Nitrate fertilization accelerates growth in cultured Tridacna , so nitrate aerosol deposition may contribute to faster growth in modern wild populations. Furthermore, colder winter temperatures and past summer monsoons may have depressed fossil giant clam growth. Giant clams can serve as sentinels of reef environmental change, both to determine their individual health and the health of the reefs they inhabit.

Heavy Metal, Rare Earth Element and Pb Isotope Dynamics in Mussels During a Depuration Experiment in the Gulf of Aqaba, Northern Red Sea

Mussels are considered highly efficient marine biomonitors, tracing anthropogenic and natural variations in heavy metals and various organic compounds. While heavy metals depuration processes in biomonitors are of growing interest, less knowledge is available regarding their Pb isotopes and rare earth elements (REEs) accumulation-release dynamics, and their response to short-term anthropogenic and terrigenous perturbations. Here, we report the results of a relocation experiment where a group of mussels (Brachidontes pharaonis) were extracted from a contaminated lagoon in the Gulf of Aqaba, northern Red Sea, and placed in water tanks that were flushed continuously with fresh, uncontaminated seawater. Specimens were removed periodically from the water table over a period of 13 weeks and trace and REEs and Pb isotopic compositions were determined separately for mussel’s shells and soft tissues. The results display a clear decrease over time in the concentrations of various heavy metals and REEs in the soft tissue, in concert with a similar shift in the Pb isotopic compositions toward seawater values. By contrast, the elemental and Pb isotopic composition of the shell presents little change over time. Coupling between the Pb isotopic composition of corresponding soft tissue and shell samples allows back-calculation of the timing and magnitude of abrupt pollution events and presents a novel approach for monitoring short-term pollution events. Nevertheless, given the coastal setting of the studied samples, it is important to consider the effects of terrigenous material on the results. Accordingly, Al-normalized element concentrations, Pb isotopes and calculated Ce anomalies, are used to identify two distinct terrigenous end members controlling the contaminated lagoon and the pristine site. The study demonstrates the potential of using mussels as robust biomonitors of natural and anthropogenic environmental perturbations through the combination between elemental concentrations and the isotopic composition of Pb.

Unravelling the Importance of Diazotrophy in Corals – Combined Assessment of Nitrogen Assimilation, Diazotrophic Community and Natural Stable Isotope Signatures

There is an increasing interest in understanding the structure and function of the microbiota associated with marine and terrestrial organisms, because it can play a major role in host nutrition and resistance to environmental stress. Reef-building corals live in association with diazotrophs, which are microbes able to fix dinitrogen. Corals are known to assimilate diazotrophically-derived nitrogen (DDN), but it is still not clear whether this nitrogen source is derived from coral-associated diazotrophs and whether it substantially contributes to the coral’s nitrogen budget. In this study, we aimed to provide a better understanding of the importance of DDN for corals using a holistic approach by simultaneously assessing DDN assimilation rates (using 15N2 tracer technique), the diazotrophic bacterial community (using nifH gene amplicon sequencing) and the natural δ15N signature in Stylophora pistillata corals from the Northern Red Sea along a depth gradient in winter and summer. Overall, our results show a discrepancy between the three parameters. DDN was assimilated by the coral holobiont during winter only, with an increased assimilation with depth. Assimilation rates were, however, not linked to the presence of coral-associated diazotrophs, suggesting that the presence of nifH genes does not necessarily imply functionality. It also suggests that DDN assimilation was independent from coral-associated diazotrophs and may instead result from nitrogen derived from planktonic diazotrophs. In addition, the δ15N signature presented negative values in almost all coral samples in both seasons, suggesting that nitrogen sources other than DDN contribute to the nitrogen budget of corals from this region. This study yields novel insight into the origin and importance of diazotrophy for scleractinian corals from the Northern Red Sea using multiple proxies.

Community Knowledge, Practice and Prevalence of Brucellosis in Livestock owners in Northern Red Sea Region, Eritrea

Background: Brucellosis is an infectious zoonotic disease. In the Sub-Saharan Africa, where the prevalence of brucellosis is not clear and the disease has been reported in most parts of Africa including Eritrea. To this date, the level of knowledge and brucellosis status in the livestock owners’ of Northern Red Sea region, Eritrea, was unknown. Therefore, the objective of this study was to evaluate the level of knowledge and practice, and determine the prevalence of brucellosis in livestock owners in the Northern Red Sea region, Eritrea.Methods: It was a descriptive community-based cross-sectional study where villages and households were selected by simple random sampling and systematic random sampling respectively. A total of twenty-nine villages and 22 livestock owners from each village were selected randomly. Blood samples were screened using Rose Bengal plate test and positives further confirmed using competitive enzyme linked immunosorbent assay techniques. Predesigned structured questionnaire was used as data collection tool. A written informed consent was obtained from each study participants. Data were entered into CSPro and analyzed by SPSS software. Results were presented in percent and frequencies.Results: A total of 637 respondents were enrolled in the study with a mean age of 42.96. Respondents were dominated by males (74.9%) and Tigre (73%) in ethnicity. Majority of them were illiterate (55.5%) and 70.5% were farmers. The overall sero-prevalence of brucellosis in the selected community was found to be 1.8% with the highest prevalence in Gindae (3.9%) followed by Shieb (3.6%) subregion. The comprehensive good knowledge and practice was rated as 58.4% and 10.5% respectively. A significant association was also shown with age, ethnicity, religion, level of education, occupation and subregions of the respondents (p <0.001). Conclusion: The overall sero-prevalence of brucellosis in the livestock owners in the study region was generally low. However, they had lower level of knowledge and very poor practice. Their level of knowledge and practice showed significant association with their age, ethnicity, level of education and sub-regions. Increasing community awareness, introducing Brucella vaccination to animals and strict controlling of selling of unpasteurized milk to the consumers are highly recommended.

Fast and pervasive transcriptomic resilience and acclimation of extremely heat-tolerant coral holobionts from the northern Red Sea

Corals from the northern Red Sea and Gulf of Aqaba exhibit extreme thermal tolerance. To examine the underlying gene expression dynamics, we exposed Stylophora pistillata from the Gulf of Aqaba to short-term (hours) and long-term (weeks) heat stress with peak seawater temperatures ranging from their maximum monthly mean of 27 °C (baseline) to 29.5 °C, 32 °C, and 34.5 °C. Corals were sampled at the end of the heat stress as well as after a recovery period at baseline temperature. Changes in coral host and symbiotic algal gene expression were determined via RNA-sequencing (RNA-Seq). Shifts in coral microbiome composition were detected by complementary DNA (cDNA)-based 16S ribosomal RNA (rRNA) gene sequencing. In all experiments up to 32 °C, RNA-Seq revealed fast and pervasive changes in gene expression, primarily in the coral host, followed by a return to baseline gene expression for the majority of coral (>94%) and algal (>71%) genes during recovery. At 34.5 °C, large differences in gene expression were observed with minimal recovery, high coral mortality, and a microbiome dominated by opportunistic bacteria (including Vibrio species), indicating that a lethal temperature threshold had been crossed. Our results show that the S. pistillata holobiont can mount a rapid and pervasive gene expression response contingent on the amplitude and duration of the thermal stress. We propose that the transcriptomic resilience and transcriptomic acclimation observed are key to the extraordinary thermal tolerance of this holobiont and, by inference, of other northern Red Sea coral holobionts, up to seawater temperatures of at least 32 °C, that is, 5 °C above their current maximum monthly mean.