Histopathological of the hepatopancreas of Pacific white shrimp (Litopenaeus vannamei) infected by white feces disease

The high production of Pacific white shrimp (Litopenaeus vannamei) in Pidie Jaya impacts water quality decline around the ponds due to aquaculture waste. This condition causes shrimp to be susceptible to diseases, one of which is white feces disease (WFD). The objective of the study is to determine the level of hepatopancreatic damage caused by WFD through histopathological performance. Four shrimp samples were taken randomly from two semi-intensive ponds in Meurah Dua District, Pidie Jaya Regency, Aceh, Indonesia. The results showed that the histopathology in the hepatopancreas was severely damaged. The damages include vacuolization, fat degeneration, and necrosis in ponds with higher ammonia concentrations. According to pH, temperature, salinity, and ammonia concentrations, ponds near residential areas were more polluted than ponds far from residential areas. This condition is projected by the more damaged histopathological obstruction of the shrimp’s hepatopancreas.

Agricultural and Aquaculture Wastes as Concrete Components: A Review

The application of agricultural and aquaculture waste in concrete greatly reduces the pressure on the ecological environment brought by traditional concrete production. The use of agricultural and aquaculture wastes as cement replacement, aggregate replacement and fiber reinforcement has showed great potential. Making full use of these wastes can help the development of sustainable concrete. This paper provides an objective evaluation and summary of agricultural waste and aquaculture waste in green concrete. Agricultural waste is divided into natural plant fiber, agricultural waste ash and multi-application waste according to useful function and alternative methods, such as sisal fiber, olive waste ash, and bamboo. Aquaculture waste mainly refers to some shells such as oyster shell. This paper analyzes the advantages and disadvantages of agricultural and aquaculture waste concrete applications that have been reported and shows how different agricultural and aquaculture wastes are made in concrete. The selection of appropriate treatment methods and usage scenarios is extremely important for agricultural and aquaculture waste concrete, which can determine whether the concrete has reliable performance. This paper will lay a foundation for the progress of waste concrete and provide reliable help for the development of environmental protection concrete.

Comparative Assessment of the Bioremediation Potential of Polychaetes (Lug worm-Arenicola marina sp and Syllidae Worm-Syllis prolifera sp) in Aquaculture Pond Sediment

Polychaetes play an important role in nutrient cycling and remediation of coastal ecosystems. Large quantities of organic matter that could lead to pollution of pond and coastal waters are generated by aquaculture waste. To assess the remediation prospects of Arenicola marina and Syllis prolifera species, laboratory sediment microcosm experiments were conducted where large size Arenicola marina and Syllis prolifera were introduced to sediment in microcosm A, large size Arenicola marina to sediment in microcosm B, large sized Syllis prolifera to sediment in microcosm C and no polychaetes to sediment in microcosm D. Microcosm A′, B′ and C′ as replicates for small size polychaetes were also set up, respectively. After 30 days, microcosm A, B and C had significant decrease in organic carbon levels with microcosm B being the highest (Total organic carbon (TOC); 27.87%; p< 0.05). Both large and small polychaetes promoted significant decrease in sulphur (S) content (mean=62.76±0.21; 62.81±0.21%) and iron(Fe) (mean=49.43±1.47; 36.28±5.90%) respectively. Increase in pH by 31.15±0.13% was found in the presence of large size polychaetes, most likely associated with the burrowing process involving oxidation of Fe to Fe2O3. Large size polychaetes had better survival (mean=92±0.82%) than their small size counterpart (mean=55±4.08%). The extent of biodegradation B>A>C>D observed revealed that large size Arenicola marina was a better bioremediator of organic matter (OM), Fe and S enriched aquaculture pond sediment, probably due to its biological characteristics, well suited for the aquaculture than other species of sea worms that produce free swimming larvae. Therefore, large size Arenicola marina significantly improved sediment quality as well as increased its pH without compromising their survival. As the search for a better bioremediator of organically enriched sediment continues, our result revealed large size Arenicola marina as a more promising candidate compared to other species documented elsewhere in the world. Hence, rearing of large size Arenicola marina sp is recommended as their feeding habits are well suited for aquaculture.

A novel application of an aquaculture waste-derived nanocomposite as a sealant

The use of renewable resources has been gaining interest due to their high economic benefits. They are attractive as a sustainable alternative to conventional resources for producing useful and valuable materials. The paper focuses on using a composite of graphene-oxide and chitosan, an aquaculture waste-derived material, as a renewable feedstock for producing a sealant for healing micro-cracks in concrete. The sealant named Eco-Nanoseal is well characterised, and its interaction with concrete is studied. A possible binding mechanism of Eco-Nanoseal with concrete is also proposed. The nanofibrous film-forming composite can solidify in the concrete environment quickly without any external polymerising agent. The quick ability to form a solid plug and strong bonding with concrete surface makes Eco-Nano seal a potential candidate for healing micro-cracks in concrete. The novel application is demonstrated well in M25 grade concrete cube specimens. With low environmental factor (E-factor) and process mass intensity (PMI) values of 0.05 and nearly 1, respectively, the Eco-Nanoseal complies the critical parameters of materials’ sustainability indices. The Eco-Nanoseal is a promising and environmentally-friendly alternative to synthetic polymer-based adhesives.

Growth Performance and Nutrient Composition of Mustard Green (Brassica juncea) cultured in Aquaponics Systems and Hydroponic System

Aquaculture waste is rich in various nutrient contents from uneaten feed, faeces or urine including nitrogen in terms of total ammonium nitrogen (TAN) and nitrite. With the help of nitrifying bacteria, the nitrogen wastes can be converted into nitrate which is one of the main components of commercial fertilizer in agriculture. This study aimed at comparing the growth and nutrient contents (crude protein, crude fat, energy, and antioxidant) of mustard Green (Brassica juncea) cultured in different culture media (aquaculture waste which is generally known as the aquaponics system vs hydroponic system which used commercial inorganic fertilizer). The aquaponics system was prepared by previously growing Nile tilapia fingerling, Oreochromis niloticus, for ~2 weeks to reach nitrate concentration on the effluent water ~30mg/L. Mustard Green was cultured in the system for 30 days. Nutrient availability in both systems was also monitored by measuring nitrate content and total dissolved solids. The results showed that the growth, nutrient content including crude protein, crude fat, total energy, and antioxidant content of the vegetable were not significantly different between the system, p>0.05. Nutrient availability especially in terms of nitrate was also not significantly different between the culture system. These results suggest that the aquaponics system could be used to produce vegetables with the same growth and nutrient content as a vegetable grown in the aquaponics system. Furthermore, aquaculture waste can be used to replace inorganic fertilizer to grow vegetables which later contribute to the reduction of total production cost.

APPLICATION OF DEPOSITION BACK IN THE AQUAPONIC SYSTEM IN REDUCING NH3 IN CULTIVATION Tilapia (Oreochromis niloticus)

The success of a culture depends on the quantity and quality of water used in fish culture without water exchange, the concentration of aquaculture waste such as ammonia (NH3) will increase very rapidly and is toxic to the cultivated organism. One of the innovations applied in fish farming is integrating plants through an aquaponics system. This study aims to determine the effect of using a settling tub in reducing ammonia waste (NH3) and growth in tilapia aquaculture using an aquaponic tidal system. The size of the initial tilapia seeds used is 3-4 cm. There are two treatments used, without settling tub (A) and addition of settling tub (B) with three replications. However, the results showed no significant differences (P>0.05) between treatments for all parameters observed. It is necessary to carry out further research on different fish and plants to see the optimization in reducing ammonia waste (NH3).