scholarly journals Untargeted Metabolomics Analysis of the Serum Metabolic Signature of Childhood Obesity

Nutrients ◽  
2022 ◽  
Vol 14 (1) ◽  
pp. 214
Author(s):  
Lukasz Szczerbinski ◽  
Gladys Wojciechowska ◽  
Adam Olichwier ◽  
Mark A. Taylor ◽  
Urszula Puchta ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Childhood Obesity ◽  
Adult Population ◽  
Normal Weight ◽  
Untargeted Metabolomics ◽  
Gas Chromatography Mass Spectrometry ◽  
Serum Samples ◽  
Metabolic Signature ◽  
Metabolomic Profiling ◽  
Altered Lipid

Obesity rates among children are growing rapidly worldwide, placing massive pressure on healthcare systems. Untargeted metabolomics can expand our understanding of the pathogenesis of obesity and elucidate mechanisms related to its symptoms. However, the metabolic signatures of obesity in children have not been thoroughly investigated. Herein, we explored metabolites associated with obesity development in childhood. Untargeted metabolomic profiling was performed on fasting serum samples from 27 obese Caucasian children and adolescents and 15 sex- and age-matched normal-weight children. Three metabolomic assays were combined and yielded 726 unique identified metabolites: gas chromatography–mass spectrometry (GC–MS), hydrophilic interaction liquid chromatography coupled to mass spectrometry (HILIC LC–MS/MS), and lipidomics. Univariate and multivariate analyses showed clear discrimination between the untargeted metabolomes of obese and normal-weight children, with 162 significantly differentially expressed metabolites between groups. Children with obesity had higher concentrations of branch-chained amino acids and various lipid metabolites, including phosphatidylcholines, cholesteryl esters, triglycerides. Thus, an early manifestation of obesity pathogenesis and its metabolic consequences in the serum metabolome are correlated with altered lipid metabolism. Obesity metabolite patterns in the adult population were very similar to the metabolic signature of childhood obesity. Identified metabolites could be potential biomarkers and used to study obesity pathomechanisms.

scholarly journals Muscle and Serum Metabolomics for Different Chicken Breeds under Commercial Conditions by GC–MS

Foods ◽  
2021 ◽  
Vol 10 (9) ◽  
pp. 2174
Author(s):  
Chengkeng Tan ◽  
Jinap Selamat ◽  
Nuzul Noorahya Jambari ◽  
Rashidah Sukor ◽  
Suganya Murugesu ◽  
...  
Keyword(s):  
Principal Component ◽  
Pectoralis Major ◽  
Untargeted Metabolomics ◽  
Gas Chromatography Mass Spectrometry ◽  
Serum Samples ◽  
Food Fraud ◽  
Village Chicken ◽  
Premium Price ◽  
Chicken Breeds ◽  
Serum Metabolomics

Globally, village chicken is popular and is known as a premium meat with a higher price. Food fraud can occur by selling other chicken breeds at a premium price in local markets. This study aimed to distinguish local village chicken from other chicken breeds available in the market, namely, colored broiler (Hubbard), broiler (Cobb), and spent laying hen (Dekalb) in pectoralis major and serum under commercial conditions using an untargeted metabolomics approach. Both pectoralis major and serum were analyzed using gas chromatography–mass spectrometry (GC–MS). The principal component analysis (PCA) results distinguished four different chicken breeds into three main groups for pectoralis major and serum. A total of 30 and 40 characteristic metabolites were identified for pectoralis major and serum, respectively. The four chicken breeds were characterized by the abundance of metabolites such as amino acids (L−glutamic acid, L−threonine, L−serine, L−leucine), organic acids (L−lactic acid, succinic acid, 3−hydroxybutyric acid), sugars (D−allose, D−glucose), sugar alcohols (myo−inositol), and fatty acids (linoleic acid). Our results suggest that an untargeted metabolomics approach using GC–MS and PCA could discriminate chicken breeds for pectoralis major and serum under commercial conditions. In this study, village chicken could only be distinguished from colored broiler (Hubbard) by serum samples.

scholarly journals Metabolite Markers for Characterizing Sasang Constitution Type through GC-MS and 1H NMR-Based Metabolomics Study

2019 ◽  
Vol 2019 ◽  
pp. 1-11 ◽  
Author(s):  
Eun-Ju Kim ◽  
Young-Shick Hong ◽  
Seung-Ho Seo ◽  
Seong-Eun Park ◽  
Chang-Su Na ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Serum Lactate ◽  
Normal Weight ◽  
Gas Chromatography Mass Spectrometry ◽  
Human Beings ◽  
Serum Samples ◽  
Sasang Constitution ◽  
Metabolomics Study ◽  
Constitution Type ◽  
Lactate Levels

Sasang constitutional medicine classifies human beings into four types based on their physical and psychological characteristics. Despite its potential value in achieving personalized medicine, the diagnosis of sasang constitution (SC) type is complex and subjective. In this study, gas chromatography–mass spectrometry and 1H nuclear magnetic resonance–based metabolic analyses were conducted to find maker metabolites in serum and urine according to different SC types. Although some samples were overlapped on orthogonal projection to latent structure discriminant analysis score plots, serum samples showed separation between different SC types. Levels of lactate, glutamate, triglyceride, and fatty acids in serum and glycolic acid in urine of Tae-Eum type were higher than those of So-Eum and So-Yang type. Fatty acids, triglyceride, and lactate levels were found to be metabolites related to body mass index, indicating that marker metabolites for the diagnosis of SC type could be associated with obese. However, Tae-Eum type showed higher lactate levels in serum than So-Yang type for both normal weight and overweight groups, suggesting that the contents of serum lactate might be dependent on the SC type regardless of body weight. These results suggest that metabolomics analysis could be used to determine SC type.

scholarly journals Determination of Mammalian Lignans in Biological Samples by Modified Gas Chromatography/Mass Spectrometry

2007 ◽  
Vol 90 (3) ◽  
pp. 641-646 ◽  
Author(s):  
Ajay Bommareddy ◽  
Bhanu L Arasada ◽  
Duane P Mathees ◽  
Chandradhar Dwivedi
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Gas Chromatography Mass Spectrometry ◽  
Serum Samples ◽  
Selected Ion Monitoring ◽  
Beneficial Effects ◽  
Chromatography Mass Spectrometry ◽  
Flaxseed Meal

Abstract Lignans in flaxseed have been part of the human diet for centuries. In 1955, the isolation and structure of the lignan derivative secoisolariciresinol diglucoside (SDG) was reported. The biological role of SDG and mammalian lignan metabolites enterodiol and enterolactone was initially reported 20 years later. Experimental evidences showed the beneficial effects of lignans on breast, colon, and thyroid cancer. A modified gas chromatography/mass spectrometry (GC/MS) assay was developed for lignans in serum and colon samples of rats fed flaxseed meal. The method developed for the analysis of metabolites involves extraction and derivatization of samples and quantitative analysis by selected ion monitoring using GC/MS. The levels of lignan metabolites enterodiol and enterolactone were determined to be 0.013 and 0.23 M in serum samples and 0.008 and 1.63 M in colon samples.

Monitoring drug concentrations in a case of combined overdosage with primidone and methsuximide.

1976 ◽  
Vol 22 (6) ◽  
pp. 915-921 ◽  
Author(s):  
G F Johnson ◽  
C J Least ◽  
J W Serum ◽  
E B Solow ◽  
H M Solomon
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Liquid Chromatography ◽  
Gas Chromatography Mass Spectrometry ◽  
Gas Liquid Chromatography ◽  
Therapeutic Decision ◽  
Serum Samples ◽  
Monitoring Drug ◽  
Drug Concentrations

Abstract We describe a case of fatal overdosage with primidone and methsuximide. During the early phase of the patient's hospital course we found concentrations of methsuximide, N-desmethylmethsuximide, and primidone in serum that far exceeded the usual therapeutic concentrations, as determined by gas-liquid chromatography. Determination of N-desmethylmethsuximide in peritoneal fluid demonstrated concentrations comparable to those in serum. This led to the therapeutic decision to manage the patient by dialysis. Subsequently, serum samples collected during the course of hospitalization were analyzed quantitatively by gas-liquid chromatography for methsuximide, N-desmethylmethsuximide, primidone, phenobarbital, and diphenylhydantoin. Selected serum specimens were also analyzed by gas chromatography-mass spectrometry, and N-methyl-2-hydroxymethyl-2-phenylsuccinimide, a metabolite of methsuximide not previously described in human serum, was identified by analysis of its mass spectrum.

scholarly journals Identification of Biomarkers of Stent Restenosis With Serum Metabolomic Profiling Using Gas Chromatography/Mass Spectrometry

2012 ◽  
Vol 76 (8) ◽  
pp. 1864-1873 ◽  
Author(s):  
Minoru Hasokawa ◽  
Masakazu Shinohara ◽  
Hiroshi Tsugawa ◽  
Takeshi Bamba ◽  
Eiichiro Fukusaki ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Gas Chromatography Mass Spectrometry ◽  
Stent Restenosis ◽  
Metabolomic Profiling ◽  
Chromatography Mass Spectrometry

scholarly journals Serum total testosterone: immunoassay compared with negative chemical ionization gas chromatography-mass spectrometry

1996 ◽  
Vol 42 (5) ◽  
pp. 749-755 ◽  
Author(s):  
R L Fitzgerald ◽  
D A Herold
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Chemical Ionization ◽  
Gas Chromatography Mass Spectrometry ◽  
Poor Correlation ◽  
Total Testosterone ◽  
Silyl Ether ◽  
Negative Chemical Ionization ◽  
Serum Samples ◽  
Chromatography Mass Spectrometry

Abstract We have developed an electron capture negative chemical ionization gas chromatography-mass spectrometry (GC-MS) procedure to quantify serum testosterone in the clinically relevant range 0.69-69.3 nmol/L and used this procedure to assess Ciba Corning Diagnostics ACS:180 testosterone immunoassay. The GC-MS method involves liquid-liquid extraction of serum samples and synthesis of a pentafluorobenzyloxime/silyl ether derivative of testosterone with excellent chromatographic and electron capturing properties. The ACS testosterone assay is the first fully automated nonradioactive testosterone immunoassay approved by the US Food and Drug Administration. Patients' specimens (101, 57 males, 44 females) were analyzed by both techniques. A plot of the GC-MS (x) vs ACS (y) testosterone concentrations for men was linear (y = 1.07x + 0.19 nmol/L), showing excellent correlation (r2 = 0.98) between the two assays. Agreement of the two assays for female specimens was poor (y = 0.72x + 1.2 nmol/L), with a poor correlation (r2 = 0.31).

scholarly journals Integrating comprehensive two-dimensional gas chromatography mass spectrometry and parallel two-dimensional liquid chromatography mass spectrometry for untargeted metabolomics

The Analyst ◽  
2019 ◽  
Vol 144 (14) ◽  
pp. 4331-4341 ◽  
Author(s):  
Md Aminul Islam Prodhan ◽  
Biyun Shi ◽  
Ming Song ◽  
Liqing He ◽  
Fang Yuan ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Liquid Chromatography ◽  
Untargeted Metabolomics ◽  
Gas Chromatography Mass Spectrometry ◽  
Two Dimensional ◽  
Liquid Chromatography Mass Spectrometry ◽  
Chromatography Mass Spectrometry

The diverse characteristics and large number of entities make metabolite separation challenging in metabolomics.

Untargeted Metabolomics Analysis of Different Grape Varieties and Different Parts of Wine Grape Using Gas Chromatography and Mass Spectrometry Technique

2021 ◽  
Vol 15 (4) ◽  
pp. 459-471
Author(s):  
Yali Wang ◽  
Lu Yang ◽  
Ke Zhang ◽  
Xiaoxiang Chen ◽  
Hangyu Wang ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Chemical Diversity ◽  
Untargeted Metabolomics ◽  
Gas Chromatography Mass Spectrometry ◽  
Wine Grape ◽  
Grape Seeds ◽  
Active Compounds ◽  
Development And Utilization ◽  
Different Parts

Natural active compounds are an important key to the development and utilization of functional foods and medicine. In this study, the chemical diversity and varietal differences of wine grape were studied by using the untargeted metabolomics method based on gas chromatography-mass spectrometry (GC-MS), and the secondary metabolites of different cultivars and different parts were analyzed. A GC-MS-based metabolomic approach and chemometrics tools was used to identify potential active compounds in Vitis vinifera. The results showed that the content of active compounds in red wine varieties was higher than that in white wine varieties, and the content of active ingredients in grape seeds was significantly higher than that in fruits. In conclusion, this study analyzed the metabolite composition and differentiation of different wine grape fruits in Turpan as a whole, and is expected to lay a foundation for the study of wine grape and to provide a theoretical basis for development and utilization of the Dietary supplement and medicine on them.

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