Damming Induced Natural Attenuation of Hydrothermal Waters by Runoff Freshwater Dilution and Sediment Biogeochemical Transformations (Sochagota Lake, Colombia)

The volcanic area of the Paipa system (Boyacá, Colombia) contains a magmatic heat source and deep fractures that help the flow of hot and highly mineralized waters, which are further combined with cold superficial inputs. This mixed water recharges the Salitre River and downstream feeding Sochagota Lake. The incoming water can contribute to substantial increases in hydrothermal SO42−-Na water in the water of the Salitre River basin area, raising the salinity. An additional hydrogeochemical process occurs in the mix with cold Fe-rich water from alluvial and surficial aquifers. This salinized Fe-rich water feeds the Sochagota Lake, although the impact of freshwaters from rain on the hydrochemistry of the Sochagota Lake is significant. A series of hydrogeochemical, biogeochemical, and mineralogical processes occur inside the lake. The aim of this work was to study the influence of damming in the Sochagota Lake, which acts as a natural attenuation of contaminants such as high concentrations of metals and salty elements coming from the Salitre River. Damming in the Sochagota Lake is considered to be an effective strategy for attenuating highly mineralized waters. The concentrations of dissolved elements were attenuated significantly. Dilution by rainfall runoff and precipitation of iron sulfides mediated by sulfate-reducing bacteria in deposits rich in organic material were the main processes involved in the attenuation of concentrations of SO42−, Fe, As Cu, and Co in the lake water. Furthermore, the K-consuming illitization processes occurring in the sediments could favor the decrease in K and Al.

Genome-Resolved Meta-Analysis of the Microbiome in Oil Reservoirs Worldwide

Microorganisms inhabiting subsurface petroleum reservoirs are key players in biochemical transformations. The interactions of microbial communities in these environments are highly complex and still poorly understood. This work aimed to assess publicly available metagenomes from oil reservoirs and implement a robust pipeline of genome-resolved metagenomics to decipher metabolic and taxonomic profiles of petroleum reservoirs worldwide. Analysis of 301.2 Gb of metagenomic information derived from heavily flooded petroleum reservoirs in China and Alaska to non-flooded petroleum reservoirs in Brazil enabled us to reconstruct 148 metagenome-assembled genomes (MAGs) of high and medium quality. At the phylum level, 74% of MAGs belonged to bacteria and 26% to archaea. The profiles of these MAGs were related to the physicochemical parameters and recovery management applied. The analysis of the potential functional core in the reservoirs showed that the microbiota was specialized for each site, with 31.7% of the total KEGG orthologies annotated as functions (1690 genes) common to all oil fields, while 18% of the functions were site-specific, i.e., present only in one of the oil fields. The oil reservoirs with a lower level of intervention were the most similar to the potential functional core, while the oil fields with a long history of water injection had greater variation in functional profile. These results show how key microorganisms and their functions respond to the distinct physicochemical parameters and interventions of the oil field operations such as water injection and expand the knowledge of biogeochemical transformations in these ecosystems.

A State-Of-The-Art Perspective on the Characterization of Subterranean Estuaries at the Regional Scale

Subterranean estuaries the, subsurface mixing zones of terrestrial groundwater and seawater, substantially influence solute fluxes to the oceans. Solutes brought by groundwater from land and solutes brought from the sea can undergo biogeochemical reactions. These are often mediated by microbes and controlled by reactions with coastal sediments, and determine the composition of fluids discharging from STEs (i.e., submarine groundwater discharge), which may have consequences showing in coastal ecosystems. While at the local scale (meters), processes have been intensively studied, the impact of subterranean estuary processes on solute fluxes to the coastal ocean remains poorly constrained at the regional scale (kilometers). In the present communication, we review the processes that occur in STEs, focusing mainly on fluid flow and biogeochemical transformations of nitrogen, phosphorus, carbon, sulfur and trace metals. We highlight the spatio-temporal dynamics and measurable manifestations of those processes. The objective of this contribution is to provide a perspective on how tracer studies, geophysical methods, remote sensing and hydrogeological modeling could exploit such manifestations to estimate the regional-scale impact of processes in STEs on solute fluxes to the coastal ocean.

Identifying source and transformation of riverine nitrates in a karst watershed, North China: comprehensively using major ions, multiple isotopes and Bayesian model

<p>The identification of nitrate (NO<sub>3</sub><sup>-</sup>) sources and biogeochemical transformations is critical for understanding and controlling diffuse pollution in surface water in drainage basins. This study combines water chemistry, environmental isotopes (δ<sup>2</sup>H<sub>H2O</sub>, δ<sup>18</sup>O<sub>H2O</sub>, δ<sup>15</sup>N<sub>NO3</sub>, and δ<sup>18</sup>O<sub>NO3</sub>), with land use data and a Bayesian isotope mixing model (Simmr), for reducing the uncertainty in estimating the contributions of different pollution sources in a Karst drainage basin of Jinan, North China. 64 samples were collected from Yufu River (YFR) of Jinan city in September and December, 2019. The results revealed that the NO<sub>3</sub><sup>−</sup>-N (4.41mg/L) was the predominant form of inorganic nitrogen in YFR watershed, accounting for about 58% of total nitrogen (8.06 mg/L). There were significant temporal and spatial variations in nitrate concentrations in the area. The nitrate concentration in time was low in December and high in September, while the process of first rising and then attenuating from upstream to downstream in space. Moreover, according to the surface water flow path, different biogeochemical transformations were observed throughout the study area: microbial nitrification was dominant in the upstream with elevated NO<sub>3</sub><sup>−</sup>-N concentrations; in the middle stream a mixing of different transformations, such as nitrification, denitrification, and/or assimilation, were identified, associated to moderate NO<sub>3</sub><sup>−</sup>-N concentrations; whereas in the downstream the main process affecting NO<sub>3</sub><sup>−</sup>-N concentrations was assimilation, and/or denitrification, resulting in low NO<sub>3</sub><sup>−</sup>-N concentrations. Water chemical and dual isotope of δ<sup>15</sup>N<sub>NO3</sub> and δ<sup>18</sup>O<sub>NO3 </sub>indicated that the river water was significantly affected by soil organic nitrogen and ammonium fertilizer inputs. Simmr mixing model outputs revealed that soil organic nitrogen (SON 55.5%) and ammonium fertilizer inputs(AF 29.5%) were the primary contributors of N pollution, whereas nitrate fertilizer(NF 7.1%), sewage & manure (M&S 3.6%), and atmospheric deposition (AP3.4%) played a less important role. The chemical fertilizer (AF and NF) and SON collectively mean contributing > 50 % of nitrate both in September and December in the watershed. Therefore, reducing fertilizer application and adopting water-saving irrigationare key to control nitrate pollution in the area. The results provide scientific basis for the water quality protection and sustainable water management in the study area or similar areas.</p>

Global patterns of nitrate isotope composition in rivers and adjacent aquifers reveal reactive nitrogen cascading

Remediation of nitrate pollution of Earth’s rivers and aquifers is hampered by cumulative biogeochemical processes and nitrogen sources. Isotopes (δ15N, δ18O) help unravel spatiotemporal nitrogen(N)-cycling of aquatic nitrate (NO3−). We synthesized nitrate isotope data (n = ~5200) for global rivers and shallow aquifers for common patterns and processes. Rivers had lower median NO3− (0.3 ± 0.2 mg L−1, n = 2902) compared to aquifers (5.5 ± 5.1 mg L−1, n = 2291) and slightly lower δ15N values (+7.1 ± 3.8‰, n = 2902 vs +7.7 ± 4.5‰, n = 2291), but were indistinguishable in δ18O (+2.3 ± 6.2‰, n = 2790 vs +2.3 ± 5.4‰, n = 2235). The isotope composition of NO3− was correlated with water temperature revealing enhanced N-cascading in warmer climates. Seasonal analyses revealed higher δ15N and δ18O values in wintertime, suggesting waste-related N-source signals are better preserved in the cold seasons. Isotopic assays of nitrate biogeochemical transformations are key to understanding nitrate pollution and to inform beneficial agricultural and land management strategies.

Strong hydroclimatic controls on vulnerability to subsurface nitrate contamination across Europe

Subsurface contamination due to excessive nutrient surpluses is a persistent and widespread problem in agricultural areas across Europe. The vulnerability of a particular location to pollution from reactive solutes, such as nitrate, is determined by the interplay between hydrologic transport and biogeochemical transformations. Current studies on the controls of subsurface vulnerability do not consider the transient behaviour of transport dynamics in the root zone. Here, using state-of-the-art hydrologic simulations driven by observed hydroclimatic forcing, we demonstrate the strong spatiotemporal heterogeneity of hydrologic transport dynamics and reveal that these dynamics are primarily controlled by the hydroclimatic gradient of the aridity index across Europe. Contrasting the space-time dynamics of transport times with reactive timescales of denitrification in soil indicate that ~75% of the cultivated areas across Europe are potentially vulnerable to nitrate leaching for at least one-third of the year. We find that neglecting the transient nature of transport and reaction timescale results in a great underestimation of the extent of vulnerable regions by almost 50%. Therefore, future vulnerability and risk assessment studies must account for the transient behaviour of transport and biogeochemical transformation processes.

Meanders as a scaling motif for understanding of floodplain soil microbiome and biogeochemical potential at the watershed scale

Biogeochemical exports of C, N, S and H2 from watersheds are modulated by the activity of microorganisms that function over micron scales. This disparity of scales presents a substantial challenge for development of predictive models describing watershed function. Here, we tested the hypothesis that meander-bound regions exhibit patterns of microbial metabolic potential that are broadly predictive of biogeochemical processes in floodplain soils along a river corridor. We intensively sampled floodplain soils located in the upper, middle, and lower reaches of the East River in Colorado and reconstructed 248 draft quality genomes representative at a sub-species level. Approximately one third of the representative genomes were detected across all three locations with similar levels of abundance, and despite the very high microbial diversity and complexity of the soils, ~15% of species were detected in two consecutive years. A core floodplain microbiome was enriched in bacterial capacities for aerobic respiration, aerobic CO oxidation, and thiosulfate oxidation with the formation of elemental sulfur. We did not detect systematic patterns of gene abundance based on sampling position relative to the river. However, at the watershed scale meander-bound floodplains appear to serve as scaling motifs that predict aggregate capacities for biogeochemical transformations in floodplain soils. Given this, we conducted a transcriptomic analysis of the middle site. Overall, the most highly transcribed genes were amoCAB and nxrAB (for nitrification) followed by genes involved in methanol and formate oxidation, and nitrogen and CO2 fixation. Low soil organic carbon correlated with high activity of genes involved in methanol, formate, sulfide, hydrogen, and ammonia oxidation, nitrite oxidoreduction, and nitrate and nitrite reduction. Thus, widely represented genetic capacities did not predict in situ activity at one time point, but rather they define a reservoir of biogeochemical potential available as conditions change.

Evaluating travel time distributions of macroporous hillslope

<p>Residence and travel times of water in headwater catchments and hillslopes represent important descriptors of hydrological regime. In this study, travel time distributions were evaluated for a montane forest hillslope site using a two-dimensional dual-continuum model. The model was used to simulate the seasonal soil water regime and selected major rainfall–runoff events observed at the hillslope site. In particular, it was used to generate hillslope breakthrough curves of a fictitious conservative tracer applied at the hillslope surface in the form of the Dirac impulse. The simulated tracer breakthroughs allowed us to estimate the travel time distributions of soil water associated with the episodic subsurface stormflow, deep percolation and transpiration, yielding partial travel time distributions for the individual discharge processes. The travel time distributions determined for stormflow were dominated by the lateral component of preferential flow. The event-based stormflow median travel times ranged from 1 to 17 days. The estimated travel times were significantly affected by the temporal rainfall patterns and antecedent soil moisture distributions. The applied modeling methodology can be used for the evaluation of runoff dynamics at the hillslope and catchment scales as well as for the quantification of biogeochemical transformations of dissolved chemicals.</p>

Vegetated Ditch Habitats Provide Net Nitrogen Sink and Phosphorus Storage Capacity in Agricultural Drainage Networks Despite Senescent Plant Leaching

The utility of vegetated ditch environments as nutrient sinks in agricultural watersheds is dependent in part on biogeochemical transformations that control plant uptake and release during decomposition. We investigated nitrogen (N) and phosphorus (P) uptake and release across four P enrichment treatments in ditch mesocosms planted with rice cutgrass (Leersia oryzoides) during the summer growing and winter decomposition seasons. Measured N retention and modeled denitrification rates did not vary, but P retention significantly increased with P enrichment. At the end of the growing season, root biomass stored significantly more N and P than aboveground stem and leaf biomass. Decomposition rates were low (<10% organic matter loss) and not affected by P enrichment. Nitrogen and P export during winter did not vary across the P enrichment gradient. Export accounted for <10% of observed summer N uptake (1363 mg m−2), with denitrification potentially accounting for at least 40% of retained N. In contrast, net P retention was dependent on enrichment; in unenriched mesocosms, P uptake and release were balanced (only 25% net retention), whereas net retention increased from 77% to 88% with increasing P enrichment. Our results indicate that vegetated ditch environments have significant potential to serve as denitrification sinks, while also storing excess P in agricultural watersheds.