Telomere Length in Pig Sperm Is Related to In Vitro Embryo Development Outcomes

Telomere length has attracted much interest as a topic of study in human reproduction; furthermore, the link between sperm telomere length and fertility outcomes has been investigated in other species. This biomarker, however, has not been much explored in other animals, such as pigs, and whether it is related to sperm quality and fertility outcomes remains unknown. The present work aimed to determine the absolute value of telomere length in pig sperm, as well as its relationship to sperm quality parameters and embryo development. Telomere length was determined through quantitative fluorescence in situ hybridization (qFISH) in 23 pig sperm samples and data were correlated to quality parameters (motility, morphology, and viability) and in vitro fertilization outcomes. We found that the mean telomere length in pig sperm was 22.1 ± 3.6 kb, which is longer than that previously described in humans. Whilst telomere length was not observed to be correlated to sperm quality variables (p > 0.05), a significant correlation between telomere length and the percentage of morulae 6 days after in vitro fertilization was observed (rs = 0.559; 95%C.I. = (−0.007 to 0.854); p = 0.047). Interestingly, this correlation was not found when percentages of early blastocysts/blastocysts (rs = 0.410; 95%C.I. = (−0.200 to 0.791); p = 0.164) and of hatching/hatched blastocysts (rs = 0.356; 95%C.I. = (− 0.260 to 0.766); p = 0.233) were considered. Through the separation of the samples into two groups by the median value, statistically significant differences between samples with shorter telomeres than the median and samples with longer telomeres than the median were found regarding development to morula (11.5 ± 3.6 vs. 21.8 ± 6.9, respectively) and to early blastocyst/blastocysts (7.6 ± 1.4 vs. 17.9 ± 12.2, respectively) (p < 0.05). In the light of these results, sperm telomere length may be a useful biomarker for embryo development in pigs, as sperm with longer telomeres lead to higher rates of morulae and blastocysts.

Indigenous Lands at Risk: Identifying Global Challenges and Opportunities in the Face of Industrial Development

Indigenous Peoples’ (IP) stewardship has helped conserve biodiversity and maintain healthy ecosystems worldwide. Among many challenges to this role are mounting pressures from industrial development. By assessing the current ecological condition of Indigenous lands with their potential for future industrial development, we show that the ecological integrity of 22% (8.6 million km2) of Indigenous lands is highly threatened across five continents and 37 countries. We further find that the risk to Indigenous lands is greatest across West and Central Africa because of their high ecological threat and greater obstacles for Indigenous Peoples to realize self-determined and culturally-responsive development outcomes. Using a novel national-level framework that examines the security of IP’ rights and authority over their lands, their capacity to engage in decision-making, and support for facilitating sustainable development, we highlight potential challenges and opportunities for strategic investments and interventions to help IP safeguard their futures and rights, as well as the ecological integrity of their lands.

Improving Early Childhood Development Outcomes in Times of COVID-19: Experimental Evidence on Parental Networks and SMS Messages

This paper presents novel evidence of an intervention to foster preschool students cognitive skills during COVID-19 pandemic. We conducted a policy experiment that provided preschool student parents with a SMS text message program to support student learning at home. Taking advantage of existing parent networks, we study the direct effect of being selected to receive the SMS text messages, and the spillovers of being part of a parent network. We show that after 15 weeks of intervention, SMS text messages increase student cognitive skills by 0.11 to 0.12 standard deviations. The effect is driven by an increase of parental involvement through the proposed activities. We find no evidence that information is transferred within parent networks.

Interface between Tourism and Rural Development in Panchmul, Nepal

This study explores interfaces between tourism and rural development outcomes (constructs) in Panchmul located in Aandhikhola rural municipality of Syangja District. A comprehensive researcher strategy has been applied in this study. The necessary information was collected from 21 participants selected purposively from tourism and rural development programs. This study found functional interfaces or two ways contributions between tourism and rural development outcomes. Similarly, tourism related activities are building social capital, developing rural infrastructures, maintaining community well-being, and conserving biodiversity which are learning platforms to the neighboring villagers and others people. Panchmul can be a model village for rural development if skillful local youths are mobilized in the activities of tourism development, promotional, and management. That’s why there must be transformative interfaces between constructs thus better to apply the strategic interface model developed from this study by the local development stakeholders.

Indigenous Lands at Risk: Identifying Global Challenges and Opportunities in the Face of Industrial Development

Indigenous Peoples’ (IP) stewardship has helped conserve biodiversity and maintain healthy ecosystems worldwide. Among many challenges to this role are mounting pressures from industrial development. By assessing the current ecological condition of Indigenous lands with their potential for future industrial development, we show that the ecological integrity of 22% (8.6 million km2) of Indigenous lands is highly threatened across five continents and 37 countries. We further find that the risk to Indigenous lands is greatest across West and Central Africa because of their high ecological threat and greater obstacles for Indigenous Peoples to realize self-determined and culturally-responsive development outcomes. Using a novel national-level framework that examines the security of IP’ rights and authority over their lands, their capacity to engage in decision-making, and support for facilitating sustainable development, we highlight potential challenges and opportunities for strategic investments and interventions to help IP safeguard their futures and rights, as well as the ecological integrity of their lands.

How Does Ocean Acidification Affect the Early Life History of Zostera marina? A Series of Experiments Find Parental Carryover Can Benefit Viability or Germination

Elevated partial pressure of carbon dioxide (pCO2) as a concomitant of global climate change may facilitate the establishment of future seagrass meadows and subsequently its benefit could be incorporated into techniques to increase restoration success. In five manipulative experiments, we determined how increased CO2 affects the maturation of flowers, and the development of seeds and seedlings for the foundation species Zostera marina. Experiments tested the development from both seeds collected from non-treated flowering shoots (direct) and seeds harvested from flowering shoots after CO2 exposure (parental carryover). Flowering shoots were collected along the western coast of Sweden near the island of Skafto. The seeds produced were used in experiments conducted at Kristineberg, Sweden and Dauphin Island, AL, United States. Experiments varied in temperature (16, 18°C) and salinity (19, 33 ppt), as well as duration and magnitude of elevated CO2 exposure. Environmental conditions among experiments, such as temperature (16, 18°C) and salinity (19, 33 ppt), as well as duration and magnitude of pCO2 exposure differed. Flowering maturation, spathe number, seed production, and indicators of seed quality did not appear to be affected by 39–69 days of exposure to CO2 conditions outside of natural variability (pCO2 = 1547.2 ± 267.60 μatm; pHT = 7.53 ± 0.07). Yet, seeds produced from these flowers showed twofold greater germination success. In another experiment, flowering shoots were exposed to an extreme CO2 condition (pCO2 = 5950.7 ± 1,849.82 μatm; pHT = 6.96 ± 0.15). In this case, flowers generated seeds that demonstrated a fivefold increase in an indicator for seed viability (sinking velocity). In the latter experiment, however, germination appeared unaffected. Direct CO2 effects on germination and seedling production were not observed. Our results provide evidence of a parental CO2 effect that can benefit germination or seed viability, but early benefits may not lead to bed establishment if other environmental conditions are not well suited for seedling development. Outcomes have implications for restoration; CO2 can be supplied to flowering shoot holding tanks to bolster success when the purpose is to redistribute seeds to locations where beds are extant and water quality is adequate.

Place-based approaches to improve health and development outcomes in young children: A scoping review

This scoping review examines the strength of evidence for the effectiveness of public policy-led place-based initiatives designed to improve outcomes for disadvantaged children, their families and the communities in which they live. Study designs and methods for evaluating such place-based initiatives were assessed, along with the contexts in which initiatives were implemented and evaluated. Thirty-two reports relating to 12 initiatives were included. Eleven initiatives used a quasi-experimental evaluation to assess impact, although there were considerable design variations within this. The remaining initiative used a pre- and post- evaluation design. Place-based initiatives by definition aim to improve multiple and interrelated outcomes. We examined initiatives to determine what outcomes were measured and coded them within the five domains of pregnancy and birth, child, parent, family and community. Across the 83 outcomes reported in the 11 studies with a comparison group, 30 (36.4%) demonstrated a positive outcome, and all but one initiative demonstrated a positive outcome in at least one outcome measure. Of the six studies that examined outcomes more than once post baseline, 10 from 38 outcomes (26.3%) demonstrated positive sustained results. Many initiatives were affected by external factors such as policy and funding changes, with unknown impact on their effectiveness. Despite the growth of place-based initiatives to improve outcomes for disadvantaged children, the evidence for their effectiveness remains inconclusive.

One Road to Riches?

Building effective state institutions before introducing democracy is widely presumed to improve different development outcomes. Conversely, proponents of this “stateness-first” argument anticipate that democratization before state building yields poor development outcomes. In this Element, we discuss several strong assumptions that (different versions of) this argument rests upon and critically evaluate the existing evidence base. In extension, we specify various observable implications. We then subject the stateness-first argument to multiple tests, focusing on economic growth as an outcome. First, we conduct historical case studies of two countries with different institutional sequencing histories, Denmark and Greece, and assess the stateness-first argument (e.g., by using a synthetic control approach). Thereafter, we draw on an extensive global sample of about 180 countries, measured across 1789–2019 and leverage panel regressions, preparametric matching, and sequence analysis to test a number of observable implications. Overall, we find little evidence to support the stateness-first argument.